(Circular) (Six-base) MAPSORT of: pFN21AB9546.seq Check: 7025 from: 1 to: 6172 pFN21AB9546 6172 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2993 2993 Size: 6172 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 1937 Size: 1578 4594 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 3471 4299 4672 1563 Size: 1908 828 373 3063 Fragments arranged by size: 3063 1908 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4235 5619 5887 1632 Size: 2603 1384 268 1917 Fragments arranged by size: 2603 1917 1384 268 AfeI AGC'GCT Cuts at: 2730 2730 Size: 6172 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 5071 6048 1203 Size: 3868 977 1327 Fragments arranged by size: 3868 1327 977 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 2144 2507 1166 Size: 978 363 4831 Fragments arranged by size: 4831 978 363 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3212 1184 Size: 2028 4144 AlwNI CAG_nnn'CTG Cuts at: 2150 3175 3217 3915 5487 2150 Size: 1025 42 698 1572 2835 Fragments arranged by size: 2835 1572 1025 698 42 ApaI G_GGCC'C Cuts at: 3331 3411 3331 Size: 80 6092 ApaLI G'TGCA_C Cuts at: 3125 4229 5385 3125 Size: 1104 1156 3912 Fragments arranged by size: 3912 1156 1104 ApoI r'AATT_y Cuts at: 1493 3481 3707 3859 5968 1493 Size: 1988 226 152 2109 1697 Fragments arranged by size: 2109 1988 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2260 2603 2705 2989 3497 1870 Size: 73 81 236 343 102 284 508 4545 Fragments arranged by size: 4545 508 343 284 236 102 81 73 BamHI G'GATC_C Cuts at: 3502 3869 3502 Size: 367 5805 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2981 3034 3063 3299 3493 Size: 325 217 627 1194 53 29 236 194 Cuts at: 3493 4948 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1194 627 325 236 217 194 53 29 BanII G_rGCy'C Cuts at: 1391 2412 2656 3331 3411 3491 1391 Size: 1021 244 675 80 80 4072 Fragments arranged by size: 4072 1021 675 244 80 80 BbeI G_GCGC'C Cuts at: 3038 3038 Size: 6172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2685 3278 928 Size: 971 786 593 3822 Fragments arranged by size: 3822 971 786 593 BbvCI CC'TCA_GC Cuts at: 3005 3005 Size: 6172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 1903 Size: 34 2426 34 3678 Fragments arranged by size: 3678 2426 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2492 3297 4783 Size: 107 122 71 631 605 820 805 1486 Cuts at: 4783 136 Size: 1525 Fragments arranged by size: 1525 1486 820 805 631 605 122 107 71 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 2111 2432 3589 2111 Size: 321 1157 4694 Fragments arranged by size: 4694 1157 321 Bme1580I G_kGCm'C Cuts at: 1790 3059 3102 3129 3302 3331 3411 4233 5389 Size: 1269 43 27 173 29 80 822 1156 Cuts at: 5389 1790 Size: 2573 Fragments arranged by size: 2573 1269 1156 822 173 80 43 29 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3117 3298 3903 Size: 416 53 83 399 24 1700 181 605 Cuts at: 3903 4861 442 Size: 958 1753 Fragments arranged by size: 1753 1700 958 605 416 399 181 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2849 3035 3239 3284 3347 4832 1239 Size: 1610 186 204 45 63 1485 2579 Fragments arranged by size: 2579 1610 1485 204 186 63 45 Bpu10I CC'TnA_GC Cuts at: 2655 3005 2655 Size: 350 5822 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 2571 3011 4297 5162 5460 2054 Size: 517 440 1286 865 298 2766 Fragments arranged by size: 2766 1286 865 517 440 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2825 4835 882 Size: 617 1326 2010 2219 Fragments arranged by size: 2219 2010 1326 617 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 6166 6166 Size: 6172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3035 3242 3979 4361 Size: 53 83 186 936 1502 207 737 382 Cuts at: 4361 4996 275 Size: 635 1451 Fragments arranged by size: 1502 1451 936 737 635 382 207 186 83 53 BsaWI w'CCGG_w Cuts at: 4605 5003 5277 5424 5835 4605 Size: 398 274 147 411 4942 Fragments arranged by size: 4942 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2190 2220 1178 Size: 30 165 30 787 30 5130 Fragments arranged by size: 5130 787 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2537 2651 3176 3236 1186 Size: 1351 114 525 60 4122 Fragments arranged by size: 4122 1351 525 114 60 BseYI C'CCAG_C Cuts at: 2330 2335 2474 2498 5375 5871 2330 Size: 5 139 24 2877 496 2631 Fragments arranged by size: 2877 2631 496 139 24 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 3272 1163 Size: 573 876 660 4063 Fragments arranged by size: 4063 876 660 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2627 3622 4383 5411 1540 Size: 275 186 626 995 761 1028 2301 Fragments arranged by size: 2301 1028 995 761 626 275 186 BsiHKAI G_wGCw'C Cuts at: 2612 2656 2940 3129 3210 4233 4318 5389 2612 Size: 44 284 189 81 1023 85 1071 3395 Fragments arranged by size: 3395 1071 1023 284 189 85 81 44 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2412 2612 2656 2940 3059 3102 3129 Size: 399 622 200 44 284 119 43 27 Cuts at: 3129 3210 3302 3331 3411 3491 4233 4318 5389 Size: 81 92 29 80 80 742 85 1071 Cuts at: 5389 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 622 399 284 200 119 92 85 81 80 80 44 43 29 27 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 4061 5791 4061 Size: 1730 4442 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2976 2993 3527 844 Size: 2132 17 534 3489 Fragments arranged by size: 3489 2132 534 17 BsrBI CCG'CTC Cuts at: 1394 1823 2201 2785 3226 4059 1394 Size: 429 378 584 441 833 3507 Fragments arranged by size: 3507 833 584 441 429 378 BsrDI GCAATG_nn' Cuts at: 65 1248 2485 4667 4841 65 Size: 1183 1237 2182 174 1396 Fragments arranged by size: 2182 1396 1237 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 2271 2682 3231 4816 5003 1700 Size: 110 209 252 411 549 1585 187 2869 Fragments arranged by size: 2869 1585 549 411 252 209 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 2048 1925 Size: 123 6049 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 3175 794 Size: 2381 3791 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 2740 3367 1184 Size: 1556 627 3989 Fragments arranged by size: 3989 1556 627 BstYI r'GATC_y Cuts at: 1972 3502 3869 4254 4271 5723 5809 5821 6167 Size: 1530 367 385 17 1452 86 12 346 Cuts at: 6167 1972 Size: 1977 Fragments arranged by size: 1977 1530 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2163 2214 2163 Size: 51 6121 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2096 2310 2490 2745 513 Size: 552 939 33 59 214 180 255 3940 Fragments arranged by size: 3940 939 552 255 214 180 59 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5433 Fragments arranged by size: 5433 683 56 BtsI GCAGTG_nn' Cuts at: 2139 2151 2211 2443 3196 3255 3395 3688 4482 Size: 12 60 232 753 59 140 293 794 Cuts at: 4482 4502 2139 Size: 20 3809 Fragments arranged by size: 3809 794 753 293 232 140 60 59 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2567 5179 809 Size: 507 1251 2612 1802 Fragments arranged by size: 2612 1802 1251 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2273 2624 Size: 54 1425 50 275 18 357 86 351 Cuts at: 2624 3117 3402 3619 4508 6137 8 Size: 493 285 217 889 1629 43 Fragments arranged by size: 1629 1425 889 493 357 351 285 275 217 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2624 3619 1537 Size: 275 812 995 4090 Fragments arranged by size: 4090 995 812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2783 3153 4102 1353 Size: 1430 370 949 3423 Fragments arranged by size: 3423 1430 949 370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2654 2654 Size: 6172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2849 3035 3239 3284 3347 4235 4832 Size: 393 1217 186 204 45 63 888 597 Cuts at: 4832 5619 5887 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1217 888 787 597 393 268 204 186 63 45 EcoNI CCTnn'n_nnAGG Cuts at: 2179 2179 Size: 6172 EcoO109I rG'GnC_Cy Cuts at: 2159 2398 2902 2994 3328 3408 2159 Size: 239 504 92 334 80 4923 Fragments arranged by size: 4923 504 334 239 92 80 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2033 2381 2732 3038 5319 1678 Size: 355 348 351 306 2281 2531 Fragments arranged by size: 2531 2281 355 351 348 306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 4813 4845 4887 Size: 32 135 32 1055 32 2154 32 42 Cuts at: 4887 4919 1373 Size: 32 2626 Fragments arranged by size: 2626 2154 1055 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3516 3762 669 Size: 988 281 1578 246 3079 Fragments arranged by size: 3079 1578 988 281 246 HindIII A'AGCT_T Cuts at: 748 2124 748 Size: 1376 4796 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KasI G'GCGC_C Cuts at: 3034 3034 Size: 6172 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2880 5286 5470 1305 Size: 1575 2406 184 2007 Fragments arranged by size: 2406 2007 1575 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3119 10 Size: 54 1425 343 1287 3063 Fragments arranged by size: 3063 1425 1287 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2507 3132 4130 4489 4648 518 Size: 648 978 363 625 998 359 159 2042 Fragments arranged by size: 2042 998 978 648 625 363 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2431 2820 3009 3217 3358 3588 4265 Size: 492 333 389 189 208 141 230 677 Cuts at: 4265 5413 5658 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 492 389 333 245 230 208 189 141 NaeI GCC'GGC Cuts at: 2021 2021 Size: 6172 NarI GG'CG_CC Cuts at: 3035 3035 Size: 6172 NcoI C'CATG_G Cuts at: 513 1065 2004 2490 513 Size: 552 939 486 4195 Fragments arranged by size: 4195 939 552 486 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NgoMIV G'CCGG_C Cuts at: 2019 2019 Size: 6172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 2624 3619 2624 Size: 995 5177 NspI r_CATG'y Cuts at: 1132 1229 3530 5075 1132 Size: 97 2301 1545 2229 Fragments arranged by size: 2301 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3460 1348 Size: 2112 4060 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 3217 1108 Size: 2109 4063 PfoI T'CCnGG_A Cuts at: 2826 2826 Size: 6172 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2159 2902 2159 Size: 743 5429 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PspOMI G'GGCC_C Cuts at: 3327 3407 3327 Size: 80 6092 PstI C_TGCA'G Cuts at: 830 2016 2146 2973 3201 3524 830 Size: 1186 130 827 228 323 3478 Fragments arranged by size: 3478 1186 827 323 228 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 3009 3217 3358 3009 Size: 208 141 5823 Fragments arranged by size: 5823 208 141 SacI G_AGCT'C Cuts at: 2656 2656 Size: 6172 SacII CC_GC'GG Cuts at: 2099 2099 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 2012 2142 2815 2969 3197 3520 4655 Size: 219 967 130 673 154 228 323 1135 Cuts at: 4655 5336 5527 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 967 681 673 323 228 219 191 154 130 SfoI GGC'GCC Cuts at: 3036 3036 Size: 6172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 2262 2991 3499 1872 Size: 390 729 508 4545 Fragments arranged by size: 4545 729 508 390 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2586 3026 4276 5177 5439 Size: 197 926 126 517 440 1250 901 262 Cuts at: 5439 820 Size: 1553 Fragments arranged by size: 1553 1250 926 901 517 440 262 197 126 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 2736 3077 1517 Size: 1219 341 4612 Fragments arranged by size: 4612 1219 341 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 3039 3611 513 Size: 552 939 486 549 572 3074 Fragments arranged by size: 3074 939 572 552 549 486 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2293 2849 2942 3040 3155 3317 4199 Size: 1255 713 556 93 98 115 162 882 Cuts at: 4199 4216 4369 4554 4999 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 882 713 556 445 185 162 153 115 98 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4418 6130 96 Size: 274 80 33 51 1443 2441 1712 138 Fragments arranged by size: 2441 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2190 2222 6132 6164 2190 Size: 32 3910 32 2198 Fragments arranged by size: 3910 2198 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2752 3424 2752 Size: 672 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 2260 2989 3497 1870 Size: 390 729 508 4545 Fragments arranged by size: 4545 729 508 390 XmnI GAAnn'nnTTC Cuts at: 4301 4301 Size: 6172 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI