################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI BlnI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmFI BsmI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI StuI SwaI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### AccI 802 GTAGAC GT^[AC][GT]AC AflII 414 CTTAAG C^TTAAG AlwNI 488 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1054 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 127 CTCGAG C^[CT]CG[GA]G AvaII 240 GGTCC G^G[AT]CC BanII 455 GAGCCC G[GA]GC[CT]^C BbrPI 73 CACGTG CAC^GTG BbvI 92 GCTGC GCAGC(8/12) BcnI 167 CCGGG CC^[GC]GG BfrI 414 CTTAAG C^TTAAG BglII 1065 AGATCT A^GATCT BlpI 440 GCTTAGC GC^T[ACGT]AGC BmyI 455 GAGCCC G[AGT]GC[ACT]^C BpmI 97 CTCCAG CTGGAG(16/14) Bpu1102I 440 GCTTAGC GC^T[ACGT]AGC BsaAI 73 CACGTG [CT]AC^GT[GA] BsaBI 1066 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 485 GTGCAG GTGCAG(16/14) BsmBI 407 GAGACG CGTCTC(1/5) BsoBI 127 CTCGAG C^[CT]CG[GA]G Bsp1286I 455 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 1116 TGTACA T^GTACA BsrGI 1116 TGTACA T^GTACA BstAPI 151 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 440 GCTTAGC GC^T[ACGT]AGC Cfr13I 240 GGTCC G^G[ACGT]CC DraII 239 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 291 CTCTTC CTCTTC(1/4) EcoO109I 239 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 1088 CCAAGG C^C[AT][AT]GG EcoT22I 962 ATGCAT ATGCA^T FauI 445 GCGGG CCCGC(4/6) HincII 141 GTTGAC GT[CT]^[GA]AC HindII 141 GTTGAC GT[CT]^[GA]AC Ksp632I 291 CTCTTC CTCTTC(1/4) MaeII 74 ACGT A^CGT MaeIII 987 GTCAC ^GT[ACGT]AC MamI 1066 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 146 CAATTG C^AATTG MseI 415 TTAA T^TAA MspA1I 463 CAGCTG C[AC]G^C[GT]G MunI 146 CAATTG C^AATTG NciI 167 CCGGG CC^[GC]GG NheI 24 GCTAGC G^CTAGC NlaIV 327 GGCTCC GG[ACGT]^[ACGT]CC NsiI 962 ATGCAT ATGCA^T PaeR7I 127 CTCGAG C^TCGAG PleI 1093 GACTC GAGTC(4/5) PmaCI 73 CACGTG CAC^GTG PmlI 73 CACGTG CAC^GTG PpuMI 239 AGGTCCT [GA]G^G[AT]CC[CT] PstI 235 CTGCAG CTGCA^G PvuII 463 CAGCTG CAG^CTG SapI 290 GCTCTTC GCTCTTC(1/4) Sau96I 240 GGTCC G^G[ACGT]CC SbfI 234 CCTGCAGG CCTGCA^GG SfaNI 952 GATGC GCATC(5/9) SphI 960 GCATGC GCATG^C Sse8387I 234 CCTGCAGG CCTGCA^GG SspBI 1116 TGTACA T^GTACA StyI 1088 CCAAGG C^C[AT][AT]GG TaqI 128 TCGA T^CGA Tru9I 415 TTAA T^TAA TseI 92 GCTGC G^C[AT]GC Tsp45I 987 GTCAC ^GT[GC]AC TthHB8I 128 TCGA T^CGA XbaI 1062 TCTAGA T^CTAGA XhoI 127 CTCGAG C^TCGAG XmnI 1054 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 851 AAATTT [GA]^AATT[CT] 1007 GAATTT Aor51HI 89 AGCGCT AGC^GCT 113 AGCGCT ApoI 851 AAATTT [GA]^AATT[CT] 1007 GAATTT BalI 357 TGGCCA TGG^CCA 934 TGGCCA BbsI 253 GAAGAC GAAGAC(2/6) 785 GAAGAC BpuAI 253 GAAGAC GAAGAC(2/6) 785 GAAGAC BsiYI 459 CCTTCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 912 CCTTGTAGGGG BslI 459 CCTTCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 912 CCTTGTAGGGG BsmAI 130 GAGAC GTCTC(1/5) 407 GAGAC BspMI 137 GCAGGT ACCTGC(4/8) 237 GCAGGT BsrBI 33 GAGCGG CCGCTC(-3/-3) 46 GAGCGG BstYI 974 GGATCT [GA]^GATC[CT] 1065 AGATCT CfoI 90 GCGC GCG^C 114 GCGC Eco47III 89 AGCGCT AGC^GCT 113 AGCGCT HaeII 89 AGCGCT [GA]GCGC^[CT] 113 AGCGCT HapII 109 CCGG C^CGG 167 CCGG HhaI 90 GCGC GCG^C 114 GCGC HinP1I 90 GCGC G^CGC 114 GCGC HindIII 434 AAGCTT A^AGCTT 841 AAGCTT HpaII 109 CCGG C^CGG 167 CCGG MflI 974 GGATCT [GA]^GATC[CT] 1065 AGATCT MluNI 357 TGGCCA TGG^CCA 934 TGGCCA MscI 357 TGGCCA TGG^CCA 934 TGGCCA MspI 109 CCGG C^CGG 167 CCGG NspI 512 ACATGC [GA]CATG^[CT] 960 GCATGC SfcI 235 CTGCAG C^T[GA][CT]AG 800 CTGTAG TfiI 184 GATTC G^A[AT]TC 649 GAATC XhoII 974 GGATCT [GA]^GATC[CT] 1065 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AfaI AluI AlwI BfaI BsaJI BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SmlI Tsp509I TspRI