Restriction map for
pFN21AE1495
(Linear) (Six-base) MAPSORT of: KIEE1495.seq Check: 6673 from: 1 to: 723
KIEE1495.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AfeI AGC'GCT
Cuts at: 0 385 723
Size: 385 338
AleI CACnn'nnGTG
Cuts at: 0 304 433 723
Size: 304 129 290
Fragments arranged by size:
304 290 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 234 266 723
Size: 234 32 457
Fragments arranged by size:
457 234 32
ApoI r'AATT_y
Cuts at: 0 673 723
Size: 673 50
AvaI C'yCGr_G
Cuts at: 0 76 576 617 723
Size: 76 500 41 106
Fragments arranged by size:
500 106 76 41
BanI G'GyrC_C
Cuts at: 0 323 723
Size: 323 400
BbeI G_GCGC'C
Cuts at: 0 327 723
Size: 327 396
BbsI GAAGACnn'nnnn_
Cuts at: 0 524 723
Size: 524 199
BglII A'GATC_T
Cuts at: 0 314 723
Size: 314 409
BlpI GC'TnA_GC
Cuts at: 0 266 723
Size: 266 457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 245 723
Size: 245 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 238 723
Size: 227 11 485
Fragments arranged by size:
485 227 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 143 703 723
Size: 143 560 20
Fragments arranged by size:
560 143 20
BsaHI Gr'CG_yC
Cuts at: 0 324 723
Size: 324 399
BsaWI w'CCGG_w
Cuts at: 0 668 723
Size: 668 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 234 264 723
Size: 234 30 459
Fragments arranged by size:
459 234 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 723
Size: 94 629
BseYI C'CCAG_C
Cuts at: 0 11 723
Size: 11 712
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 400 436 457 539 723
Size: 127 273 36 21 82 184
Fragments arranged by size:
273 184 127 82 36 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 723
Size: 50 673
BsrBI CCG'CTC
Cuts at: 0 116 197 723
Size: 116 81 526
Fragments arranged by size:
526 116 81
BsrDI GCAATG_nn'
Cuts at: 0 260 723
Size: 260 463
BsrFI r'CCGG_y
Cuts at: 0 270 647 723
Size: 270 377 76
Fragments arranged by size:
377 270 76
BssSI C'ACGA_G
Cuts at: 0 230 723
Size: 230 493
BstEII G'GTnAC_C
Cuts at: 0 621 723
Size: 621 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 723
Size: 354 369
BstYI r'GATC_y
Cuts at: 0 314 723
Size: 314 409
BtgI C'CryG_G
Cuts at: 0 503 723
Size: 503 220
BtsI GCAGTG_nn'
Cuts at: 0 424 723
Size: 424 299
EaeI y'GGCC_r
Cuts at: 0 272 361 723
Size: 272 89 362
Fragments arranged by size:
362 272 89
EarI CTCTTCn'nnn_
Cuts at: 0 25 723
Size: 25 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 634 723
Size: 634 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 238 723
Size: 227 11 485
Fragments arranged by size:
485 227 11
EcoO109I rG'GnC_Cy
Cuts at: 0 66 723
Size: 66 657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 723
Size: 507 216
HaeII r_GCGC'y
Cuts at: 0 19 59 327 387 723
Size: 19 40 268 60 336
Fragments arranged by size:
336 268 60 40 19
KasI G'GCGC_C
Cuts at: 0 323 723
Size: 323 400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 158 723
Size: 156 2 565
Fragments arranged by size:
565 156 2
MslI CAynn'nnrTG
Cuts at: 0 304 433 534 686 723
Size: 304 129 101 152 37
Fragments arranged by size:
304 152 129 101 37
MspA1I CmG'CkG
Cuts at: 0 441 553 723
Size: 441 112 170
Fragments arranged by size:
441 170 112
NaeI GCC'GGC
Cuts at: 0 272 649 723
Size: 272 377 74
Fragments arranged by size:
377 272 74
NarI GG'CG_CC
Cuts at: 0 324 723
Size: 324 399
NgoMIV G'CCGG_C
Cuts at: 0 270 647 723
Size: 270 377 76
Fragments arranged by size:
377 270 76
NspI r_CATG'y
Cuts at: 0 533 723
Size: 533 190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 723
Size: 234 32 457
Fragments arranged by size:
457 234 32
PpuMI rG'GwC_Cy
Cuts at: 0 66 723
Size: 66 657
PvuII CAG'CTG
Cuts at: 0 441 553 723
Size: 441 112 170
Fragments arranged by size:
441 170 112
SanDI GG'GwC_CC
Cuts at: 0 66 723
Size: 66 657
SapI GCTCTTCn'nnn_
Cuts at: 0 25 723
Size: 25 698
SfoI GGC'GCC
Cuts at: 0 325 723
Size: 325 398
SmaI CCC'GGG
Cuts at: 0 578 723
Size: 578 145
SphI G_CATG'C
Cuts at: 0 533 723
Size: 533 190
StyI C'CwwG_G
Cuts at: 0 656 714 723
Size: 656 58 9
Fragments arranged by size:
656 58 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 723
Size: 445 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 510 723
Size: 510 213
XmaI C'CCGG_G
Cuts at: 0 576 723
Size: 576 147
Enzymes that do cut:
AfeI AleI AloI ApoI AvaI BanI BbeI BbsI
BglII BlpI BplI BpmI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BspMI BsrBI BsrDI BsrFI BssSI
BstEII BstXI BstYI BtgI BtsI EaeI EarI EciI
Eco57MI EcoO109I FalI HaeII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PpiI PpuMI PvuII SanDI
SapI SfoI SmaI SphI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI Bpu10I
BpuEI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI