Restriction map for pFN21AE1495
 (Linear) (Six-base) MAPSORT of: KIEE1495.seq  Check: 6673  from: 1  to: 723



KIEE1495.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AfeI AGC'GCT

Cuts at:      0     385     723 
   Size:        385     338
 
AleI CACnn'nnGTG

Cuts at:      0     304     433     723 
   Size:        304     129     290

  Fragments arranged by size: 

                 304     290     129
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     234     266     723 
   Size:        234      32     457

  Fragments arranged by size: 

                 457     234      32
 
ApoI r'AATT_y

Cuts at:      0     673     723 
   Size:        673      50
 
AvaI C'yCGr_G

Cuts at:      0      76     576     617     723 
   Size:         76     500      41     106

  Fragments arranged by size: 

                 500     106      76      41
 
BanI G'GyrC_C

Cuts at:      0     323     723 
   Size:        323     400
 
BbeI G_GCGC'C

Cuts at:      0     327     723 
   Size:        327     396
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     524     723 
   Size:        524     199
 
BglII A'GATC_T

Cuts at:      0     314     723 
   Size:        314     409
 
BlpI GC'TnA_GC

Cuts at:      0     266     723 
   Size:        266     457
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     245     723 
   Size:        245     478
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     238     723 
   Size:        227      11     485

  Fragments arranged by size: 

                 485     227      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     143     703     723 
   Size:        143     560      20

  Fragments arranged by size: 

                 560     143      20
 
BsaHI Gr'CG_yC

Cuts at:      0     324     723 
   Size:        324     399
 
BsaWI w'CCGG_w

Cuts at:      0     668     723 
   Size:        668      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     234     264     723 
   Size:        234      30     459

  Fragments arranged by size: 

                 459     234      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     723 
   Size:         94     629
 
BseYI C'CCAG_C

Cuts at:      0      11     723 
   Size:         11     712
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     400     436     457     539     723 
   Size:        127     273      36      21      82     184

  Fragments arranged by size: 

                 273     184     127      82      36      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      50     723 
   Size:         50     673
 
BsrBI CCG'CTC

Cuts at:      0     116     197     723 
   Size:        116      81     526

  Fragments arranged by size: 

                 526     116      81
 
BsrDI GCAATG_nn'

Cuts at:      0     260     723 
   Size:        260     463
 
BsrFI r'CCGG_y

Cuts at:      0     270     647     723 
   Size:        270     377      76

  Fragments arranged by size: 

                 377     270      76
 
BssSI C'ACGA_G

Cuts at:      0     230     723 
   Size:        230     493
 
BstEII G'GTnAC_C

Cuts at:      0     621     723 
   Size:        621     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     354     723 
   Size:        354     369
 
BstYI r'GATC_y

Cuts at:      0     314     723 
   Size:        314     409
 
BtgI C'CryG_G

Cuts at:      0     503     723 
   Size:        503     220
 
BtsI GCAGTG_nn'

Cuts at:      0     424     723 
   Size:        424     299
 
EaeI y'GGCC_r

Cuts at:      0     272     361     723 
   Size:        272      89     362

  Fragments arranged by size: 

                 362     272      89
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     723 
   Size:         25     698
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     634     723 
   Size:        634      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     238     723 
   Size:        227      11     485

  Fragments arranged by size: 

                 485     227      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     723 
   Size:         66     657
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     507     723 
   Size:        507     216
 
HaeII r_GCGC'y

Cuts at:      0      19      59     327     387     723 
   Size:         19      40     268      60     336

  Fragments arranged by size: 

                 336     268      60      40      19
 
KasI G'GCGC_C

Cuts at:      0     323     723 
   Size:        323     400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     158     723 
   Size:        156       2     565

  Fragments arranged by size: 

                 565     156       2
 
MslI CAynn'nnrTG

Cuts at:      0     304     433     534     686     723 
   Size:        304     129     101     152      37

  Fragments arranged by size: 

                 304     152     129     101      37
 
MspA1I CmG'CkG

Cuts at:      0     441     553     723 
   Size:        441     112     170

  Fragments arranged by size: 

                 441     170     112
 
NaeI GCC'GGC

Cuts at:      0     272     649     723 
   Size:        272     377      74

  Fragments arranged by size: 

                 377     272      74
 
NarI GG'CG_CC

Cuts at:      0     324     723 
   Size:        324     399
 
NgoMIV G'CCGG_C

Cuts at:      0     270     647     723 
   Size:        270     377      76

  Fragments arranged by size: 

                 377     270      76
 
NspI r_CATG'y

Cuts at:      0     533     723 
   Size:        533     190
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     234     266     723 
   Size:        234      32     457

  Fragments arranged by size: 

                 457     234      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      66     723 
   Size:         66     657
 
PvuII CAG'CTG

Cuts at:      0     441     553     723 
   Size:        441     112     170

  Fragments arranged by size: 

                 441     170     112
 
SanDI GG'GwC_CC

Cuts at:      0      66     723 
   Size:         66     657
 
SapI GCTCTTCn'nnn_

Cuts at:      0      25     723 
   Size:         25     698
 
SfoI GGC'GCC

Cuts at:      0     325     723 
   Size:        325     398
 
SmaI CCC'GGG

Cuts at:      0     578     723 
   Size:        578     145
 
SphI G_CATG'C

Cuts at:      0     533     723 
   Size:        533     190
 
StyI C'CwwG_G

Cuts at:      0     656     714     723 
   Size:        656      58       9

  Fragments arranged by size: 

                 656      58       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     445     723 
   Size:        445     278
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     510     723 
   Size:        510     213
 
XmaI C'CCGG_G

Cuts at:      0     576     723 
   Size:        576     147
 

 Enzymes that do cut:

     AfeI     AleI     AloI     ApoI     AvaI     BanI     BbeI     BbsI
    BglII     BlpI     BplI     BpmI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstEII    BstXI    BstYI     BtgI     BtsI     EaeI     EarI     EciI
  Eco57MI EcoO109I     FalI    HaeII     KasI     MmeI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PpiI    PpuMI    PvuII    SanDI
     SapI     SfoI     SmaI     SphI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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