(Linear) (Six-base) MAPSORT of: KIBB8414.seq Check: 6782 from: 1 to: 2739 KIBB8414.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AatII G_ACGT'C Cuts at: 0 1156 2739 Size: 1156 1583 AccI GT'mk_AC Cuts at: 0 101 367 2739 Size: 101 266 2372 Fragments arranged by size: 2372 266 101 Acc65I G'GTAC_C Cuts at: 0 1037 2739 Size: 1037 1702 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 336 1457 2465 2739 Size: 223 113 1121 1008 274 Fragments arranged by size: 1121 1008 274 223 113 AfeI AGC'GCT Cuts at: 0 2053 2739 Size: 2053 686 AflIII A'CryG_T Cuts at: 0 1397 2236 2722 2739 Size: 1397 839 486 17 Fragments arranged by size: 1397 839 486 17 AleI CACnn'nnGTG Cuts at: 0 416 1400 1795 2739 Size: 416 984 395 944 Fragments arranged by size: 984 944 416 395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1813 1920 2739 Size: 1813 107 819 Fragments arranged by size: 1813 819 107 AlwNI CAG_nnn'CTG Cuts at: 0 667 2006 2389 2739 Size: 667 1339 383 350 Fragments arranged by size: 1339 667 383 350 ApaI G_GGCC'C Cuts at: 0 201 820 2739 Size: 201 619 1919 Fragments arranged by size: 1919 619 201 ApaLI G'TGCA_C Cuts at: 0 793 2739 Size: 793 1946 ApoI r'AATT_y Cuts at: 0 1822 2245 2739 Size: 1822 423 494 Fragments arranged by size: 1822 494 423 AvaI C'yCGr_G Cuts at: 0 27 64 255 2739 Size: 27 37 191 2484 Fragments arranged by size: 2484 191 37 27 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1021 1054 2739 Size: 1021 33 1685 Fragments arranged by size: 1685 1021 33 BamHI G'GATC_C Cuts at: 0 305 2290 2739 Size: 305 1985 449 Fragments arranged by size: 1985 449 305 BanI G'GyrC_C Cuts at: 0 1037 1789 2739 Size: 1037 752 950 Fragments arranged by size: 1037 950 752 BanII G_rGCy'C Cuts at: 0 201 820 2739 Size: 201 619 1919 Fragments arranged by size: 1919 619 201 BbsI GAAGACnn'nnnn_ Cuts at: 0 1868 2739 Size: 1868 871 BbvCI CC'TCA_GC Cuts at: 0 1161 2739 Size: 1161 1578 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 622 656 659 693 2739 Size: 622 34 3 34 2046 Fragments arranged by size: 2046 622 34 34 3 BciVI GTATCCnnnnn_n' Cuts at: 0 2122 2184 2485 2739 Size: 2122 62 301 254 Fragments arranged by size: 2122 301 254 62 BclI T'GATC_A Cuts at: 0 1626 2739 Size: 1626 1113 BfrBI ATG'CAT Cuts at: 0 2111 2739 Size: 2111 628 BglI GCCn_nnn'nGGC Cuts at: 0 1171 2027 2739 Size: 1171 856 712 Fragments arranged by size: 1171 856 712 BglII A'GATC_T Cuts at: 0 537 1116 2739 Size: 537 579 1623 Fragments arranged by size: 1623 579 537 Bme1580I G_kGCm'C Cuts at: 0 201 363 797 820 2070 2739 Size: 201 162 434 23 1250 669 Fragments arranged by size: 1250 669 434 201 162 23 BmgBI CAC'GTC Cuts at: 0 576 1521 2531 2739 Size: 576 945 1010 208 Fragments arranged by size: 1010 945 576 208 BmrI ACTGGGnnnn_n' Cuts at: 0 123 357 1157 1662 1710 2739 Size: 123 234 800 505 48 1029 Fragments arranged by size: 1029 800 505 234 123 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 671 1242 1534 1736 1998 2739 Size: 671 571 292 202 262 741 Fragments arranged by size: 741 671 571 292 262 202 Bpu10I CC'TnA_GC Cuts at: 0 1161 1735 2739 Size: 1161 574 1004 Fragments arranged by size: 1161 1004 574 BsaI GGTCTCn'nnnn_ Cuts at: 0 1553 1604 2739 Size: 1553 51 1135 Fragments arranged by size: 1553 1135 51 BsaAI yAC'GTr Cuts at: 0 792 1398 2739 Size: 792 606 1341 Fragments arranged by size: 1341 792 606 BsaBI GATnn'nnATC Cuts at: 0 2496 2739 Size: 2496 243 BsaHI Gr'CG_yC Cuts at: 0 1153 2739 Size: 1153 1586 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 946 976 982 1012 1777 1807 2085 2115 Size: 946 30 6 30 765 30 278 30 Cuts at: 2115 2739 Size: 624 Fragments arranged by size: 946 765 624 278 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 734 1571 2739 Size: 734 837 1168 Fragments arranged by size: 1168 837 734 BseYI C'CCAG_C Cuts at: 0 5 394 785 812 1676 2377 2739 Size: 5 389 391 27 864 701 362 Fragments arranged by size: 864 701 391 389 362 27 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 669 1469 1819 1938 2391 2464 2739 Size: 669 800 350 119 453 73 275 Fragments arranged by size: 800 669 453 350 275 119 73 BsiHKAI G_wGCw'C Cuts at: 0 797 1414 1520 2302 2739 Size: 797 617 106 782 437 Fragments arranged by size: 797 782 617 437 106 BsmI GAATG_Cn' Cuts at: 0 734 2739 Size: 734 2005 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1220 2739 Size: 1220 1519 Bsp1286I G_dGCh'C Cuts at: 0 201 363 797 820 1414 1520 2070 2302 Size: 201 162 434 23 594 106 550 232 Cuts at: 2302 2739 Size: 437 Fragments arranged by size: 594 550 437 434 232 201 162 106 23 BspHI T'CATG_A Cuts at: 0 1661 2739 Size: 1661 1078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 50 1888 2739 Size: 50 1838 851 Fragments arranged by size: 1838 851 50 BsrDI GCAATG_nn' Cuts at: 0 1184 1671 1965 2541 2739 Size: 1184 487 294 576 198 Fragments arranged by size: 1184 576 487 294 198 BsrGI T'GTAC_A Cuts at: 0 2257 2557 2725 2739 Size: 2257 300 168 14 Fragments arranged by size: 2257 300 168 14 BstAPI GCAn_nnn'nTGC Cuts at: 0 667 2117 2405 2739 Size: 667 1450 288 334 Fragments arranged by size: 1450 667 334 288 BstEII G'GTnAC_C Cuts at: 0 2101 2739 Size: 2101 638 BstXI CCAn_nnnn'nTGG Cuts at: 0 1324 1359 2739 Size: 1324 35 1380 Fragments arranged by size: 1380 1324 35 BstYI r'GATC_y Cuts at: 0 305 537 1116 2003 2290 2739 Size: 305 232 579 887 287 449 Fragments arranged by size: 887 579 449 305 287 232 BtgI C'CryG_G Cuts at: 0 1169 1352 1634 2097 2739 Size: 1169 183 282 463 642 Fragments arranged by size: 1169 642 463 282 183 BtsI GCAGTG_nn' Cuts at: 0 411 423 681 957 1010 1457 1926 1953 Size: 411 12 258 276 53 447 469 27 Cuts at: 1953 2166 2226 2739 Size: 213 60 513 Fragments arranged by size: 513 469 447 411 276 258 213 60 53 27 12 DrdI GACnn_nn'nnGTC Cuts at: 0 742 2739 Size: 742 1997 EaeI y'GGCC_r Cuts at: 0 1064 1565 1610 2028 2334 2739 Size: 1064 501 45 418 306 405 Fragments arranged by size: 1064 501 418 405 306 45 EarI CTCTTCn'nnn_ Cuts at: 0 463 1040 2484 2627 2739 Size: 463 577 1444 143 112 Fragments arranged by size: 1444 577 463 143 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 912 1426 2659 2739 Size: 912 514 1233 80 Fragments arranged by size: 1233 912 514 80 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 336 671 1242 1457 1534 1736 1998 Size: 223 113 335 571 215 77 202 262 Cuts at: 1998 2465 2739 Size: 467 274 Fragments arranged by size: 571 467 335 274 262 223 215 202 113 77 EcoO109I rG'GnC_Cy Cuts at: 0 197 198 266 816 1704 1900 2739 Size: 197 1 68 550 888 196 839 Fragments arranged by size: 888 839 550 197 196 68 1 EcoRI G'AATT_C Cuts at: 0 2245 2739 Size: 2245 494 EcoRV GAT'ATC Cuts at: 0 2517 2739 Size: 2517 222 FspI TGC'GCA Cuts at: 0 1531 2739 Size: 1531 1208 FspAI rTGC'GCAy Cuts at: 0 1531 2739 Size: 1531 1208 HaeII r_GCGC'y Cuts at: 0 61 809 2055 2739 Size: 61 748 1246 684 Fragments arranged by size: 1246 748 684 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 516 548 946 978 1039 1071 1711 1743 Size: 516 32 398 32 61 32 640 32 Cuts at: 1743 2739 Size: 996 Fragments arranged by size: 996 640 516 398 61 32 32 32 32 HincII GTy'rAC Cuts at: 0 1506 2739 Size: 1506 1233 HindIII A'AGCT_T Cuts at: 0 1469 2739 Size: 1469 1270 KpnI G_GTAC'C Cuts at: 0 1041 2739 Size: 1041 1698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 70 333 672 2739 Size: 70 263 339 2067 Fragments arranged by size: 2067 339 263 70 MscI TGG'CCA Cuts at: 0 1066 1567 1612 2030 2739 Size: 1066 501 45 418 709 Fragments arranged by size: 1066 709 501 418 45 MslI CAynn'nnrTG Cuts at: 0 283 416 751 1322 1357 1400 1795 1958 Size: 283 133 335 571 35 43 395 163 Cuts at: 1958 2739 Size: 781 Fragments arranged by size: 781 571 395 335 283 163 133 43 35 MspA1I CmG'CkG Cuts at: 0 17 667 812 1082 2018 2156 2739 Size: 17 650 145 270 936 138 583 Fragments arranged by size: 936 650 583 270 145 138 17 NcoI C'CATG_G Cuts at: 0 1169 1352 1634 2097 2739 Size: 1169 183 282 463 642 Fragments arranged by size: 1169 642 463 282 183 NsiI A_TGCA'T Cuts at: 0 2113 2739 Size: 2113 626 NspI r_CATG'y Cuts at: 0 402 1240 2115 2240 2726 2739 Size: 402 838 875 125 486 13 Fragments arranged by size: 875 838 486 402 125 13 PciI A'CATG_T Cuts at: 0 2236 2722 2739 Size: 2236 486 17 Fragments arranged by size: 2236 486 17 PflMI CCAn_nnn'nTGG Cuts at: 0 113 401 2739 Size: 113 288 2338 Fragments arranged by size: 2338 288 113 PfoI T'CCnGG_A Cuts at: 0 140 506 2739 Size: 140 366 2233 Fragments arranged by size: 2233 366 140 PmlI CAC'GTG Cuts at: 0 792 1398 2739 Size: 792 606 1341 Fragments arranged by size: 1341 792 606 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1584 1616 2739 Size: 1584 32 1123 Fragments arranged by size: 1584 1123 32 PpuMI rG'GwC_Cy Cuts at: 0 266 1704 1900 2739 Size: 266 1438 196 839 Fragments arranged by size: 1438 839 266 196 PshAI GACnn'nnGTC Cuts at: 0 97 353 2739 Size: 97 256 2386 Fragments arranged by size: 2386 256 97 PspOMI G'GGCC_C Cuts at: 0 197 816 2739 Size: 197 619 1923 Fragments arranged by size: 1923 619 197 PstI C_TGCA'G Cuts at: 0 418 1017 1179 2059 2288 2739 Size: 418 599 162 880 229 451 Fragments arranged by size: 880 599 451 418 229 162 PvuII CAG'CTG Cuts at: 0 812 2018 2156 2739 Size: 812 1206 138 583 Fragments arranged by size: 1206 812 583 138 SanDI GG'GwC_CC Cuts at: 0 266 2739 Size: 266 2473 SbfI CC_TGCA'GG Cuts at: 0 2288 2739 Size: 2288 451 SfcI C'TryA_G Cuts at: 0 414 1013 1074 1112 1175 1848 2055 2284 Size: 414 599 61 38 63 673 207 229 Cuts at: 2284 2451 2739 Size: 167 288 Fragments arranged by size: 673 599 414 288 229 207 167 63 61 38 SmaI CCC'GGG Cuts at: 0 29 2739 Size: 29 2710 SmlI C'TyrA_G Cuts at: 0 255 2739 Size: 255 2484 StuI AGG'CCT Cuts at: 0 905 2739 Size: 905 1834 StyI C'CwwG_G Cuts at: 0 1033 1169 1352 1634 2097 2739 Size: 1033 136 183 282 463 642 Fragments arranged by size: 1033 642 463 282 183 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1433 1558 2384 2739 Size: 1433 125 826 355 Fragments arranged by size: 1433 826 355 125 TatI w'GTAC_w Cuts at: 0 2239 2257 2557 2725 2739 Size: 2239 18 300 168 14 Fragments arranged by size: 2239 300 168 18 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 31 63 630 662 1242 1274 2085 2117 Size: 31 32 567 32 580 32 811 32 Cuts at: 2117 2205 2237 2739 Size: 88 32 502 Fragments arranged by size: 811 580 567 502 88 32 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 0 1702 2739 Size: 1702 1037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 299 2739 Size: 299 2440 XhoI C'TCGA_G Cuts at: 0 255 2739 Size: 255 2484 XmaI C'CCGG_G Cuts at: 0 27 2739 Size: 27 2712 ZraI GAC'GTC Cuts at: 0 1154 2739 Size: 1154 1585 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI SbfI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: AarI AclI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BbeI BlpI BmtI BplI BpuEI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI FalI FseI HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI