(Circular) (Six-base) MAPSORT of: pFN21AB7779.seq Check: 4179 from: 1 to: 6007 pFN21AB7779 6007 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 3306 4134 4507 1563 Size: 1743 828 373 3063 Fragments arranged by size: 3063 1743 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2230 2415 2713 4070 5454 5722 1632 Size: 598 185 298 1357 1384 268 1917 Fragments arranged by size: 1917 1384 1357 598 298 268 185 AfeI AGC'GCT Cuts at: 2241 2241 Size: 6007 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 2542 2576 3051 4906 5883 1203 Size: 1339 34 475 1855 977 1327 Fragments arranged by size: 1855 1339 1327 977 475 34 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2099 1184 Size: 915 5092 AlwNI CAG_nnn'CTG Cuts at: 2035 2372 2402 2420 2969 3073 3750 5322 2035 Size: 337 30 18 549 104 677 1572 2720 Fragments arranged by size: 2720 1572 677 549 337 104 30 18 ApaLI G'TGCA_C Cuts at: 3287 4064 5220 3287 Size: 777 1156 4074 Fragments arranged by size: 4074 1156 777 ApoI r'AATT_y Cuts at: 1493 2531 2630 3007 3040 3187 3316 3542 3694 Size: 1038 99 377 33 147 129 226 152 Cuts at: 3694 5803 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1038 377 226 152 147 129 99 33 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 3332 1870 Size: 73 1389 4545 Fragments arranged by size: 4545 1389 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3328 4783 618 Size: 325 217 627 1541 1455 1842 Fragments arranged by size: 1842 1541 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2299 3326 1391 Size: 908 1027 4072 Fragments arranged by size: 4072 1027 908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5036 BbvCI CC'TCA_GC Cuts at: 2777 2777 Size: 6007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2860 3124 3289 3899 5109 2860 Size: 264 165 610 1210 3758 Fragments arranged by size: 3758 1210 610 264 165 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6002 6002 Size: 6007 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 3291 4068 5224 1790 Size: 1501 777 1156 2573 Fragments arranged by size: 2573 1501 1156 777 BmgBI CAC'GTC Cuts at: 1318 2579 1318 Size: 1261 4746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2787 3738 4696 Size: 416 53 83 399 24 1370 951 958 Cuts at: 4696 442 Size: 1753 Fragments arranged by size: 1753 1370 958 951 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 2525 4667 1239 Size: 1203 83 2142 2579 Fragments arranged by size: 2579 2142 1203 83 Bpu10I CC'TnA_GC Cuts at: 2777 2777 Size: 6007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2285 4670 882 Size: 617 786 2385 2219 Fragments arranged by size: 2385 2219 786 617 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3814 4196 4831 275 Size: 53 83 186 936 2281 382 635 1451 Fragments arranged by size: 2281 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3011 4440 4838 5112 5259 5670 3011 Size: 1429 398 274 147 411 3348 Fragments arranged by size: 3348 1429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3099 3129 1178 Size: 30 165 30 1696 30 4056 Fragments arranged by size: 4056 1696 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2307 2318 2825 1186 Size: 1121 11 507 4368 Fragments arranged by size: 4368 1121 507 11 BseYI C'CCAG_C Cuts at: 2322 5210 5706 2322 Size: 2888 496 2623 Fragments arranged by size: 2888 2623 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2934 2993 1163 Size: 573 351 847 59 4177 Fragments arranged by size: 4177 847 573 351 59 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2940 3457 4218 5246 1540 Size: 275 186 939 517 761 1028 2301 Fragments arranged by size: 2301 1028 939 761 517 275 186 BsiHKAI G_wGCw'C Cuts at: 3291 4068 4153 5224 3291 Size: 777 85 1071 4074 Fragments arranged by size: 4074 1071 777 85 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2299 3291 3326 4068 4153 5224 1391 Size: 399 509 992 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 992 742 509 399 85 35 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 2163 3057 3896 5626 2163 Size: 894 839 1730 2544 Fragments arranged by size: 2544 1730 894 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3362 844 Size: 2518 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4651 4838 1700 Size: 110 2841 187 2869 Fragments arranged by size: 2869 2841 187 110 BsrGI T'GTAC_A Cuts at: 96 3195 96 Size: 3099 2908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 2402 794 Size: 1608 4399 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstYI r'GATC_y Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002 Size: 1365 367 385 17 1452 86 12 346 Cuts at: 6002 1972 Size: 1977 Fragments arranged by size: 1977 1452 1365 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2409 513 Size: 552 939 405 4111 Fragments arranged by size: 4111 939 552 405 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3213 3311 3213 Size: 98 5909 DraIII CAC_nnn'GTG Cuts at: 2065 2065 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2056 2101 Size: 54 1425 50 275 18 207 19 45 Cuts at: 2101 2817 2937 3454 4343 5972 8 Size: 716 120 517 889 1629 43 Fragments arranged by size: 1629 1425 889 716 517 275 207 120 54 50 45 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2937 3454 1537 Size: 275 1125 517 4090 Fragments arranged by size: 4090 1125 517 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 3937 1353 Size: 896 1688 3423 Fragments arranged by size: 3423 1688 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 4594 4968 5114 387 Size: 1064 138 540 2465 374 146 1280 Fragments arranged by size: 2465 1280 1064 540 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2230 2415 2442 2525 2713 4070 4667 Size: 393 598 185 27 83 188 1357 597 Cuts at: 4667 5454 5722 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1357 787 598 597 393 268 188 185 83 27 EcoNI CCTnn'n_nnAGG Cuts at: 2376 3270 2376 Size: 894 5113 EcoO109I rG'GnC_Cy Cuts at: 2308 2369 3096 2308 Size: 61 727 5219 Fragments arranged by size: 5219 727 61 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2243 5154 1678 Size: 565 2911 2531 Fragments arranged by size: 2911 2531 565 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 4648 4680 4722 Size: 32 135 32 428 32 2616 32 42 Cuts at: 4722 4754 1373 Size: 32 2626 Fragments arranged by size: 2626 2616 428 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 2854 3606 2854 Size: 752 5255 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3085 5121 5305 1305 Size: 1780 2036 184 2007 Fragments arranged by size: 2036 2007 1780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2058 2819 10 Size: 54 1425 343 226 761 3198 Fragments arranged by size: 3198 1425 761 343 226 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 2837 3056 3062 3965 4324 4483 Size: 648 1479 192 219 6 903 359 159 Cuts at: 4483 518 Size: 2042 Fragments arranged by size: 2042 1479 903 648 359 219 192 159 6 MspA1I CmG'CkG Cuts at: 1606 2402 2411 2948 2969 3423 4100 5248 5493 Size: 796 9 537 21 454 677 1148 245 Cuts at: 5493 1606 Size: 2120 Fragments arranged by size: 2120 1148 796 677 537 454 245 21 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4516 Fragments arranged by size: 4516 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2213 2526 2546 3055 3067 3365 4910 Size: 97 984 313 20 509 12 298 1545 Cuts at: 4910 1132 Size: 2229 Fragments arranged by size: 2229 1545 984 509 313 298 97 20 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 2542 3051 4906 2542 Size: 509 1855 3643 Fragments arranged by size: 3643 1855 509 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 3044 1108 Size: 1077 859 4071 Fragments arranged by size: 4071 1077 859 PfoI T'CCnGG_A Cuts at: 2018 2018 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2369 3096 2308 Size: 61 727 5219 Fragments arranged by size: 5219 727 61 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 2031 2407 3359 830 Size: 1201 376 952 3478 Fragments arranged by size: 3478 1201 952 376 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2402 2948 2969 2402 Size: 546 21 5440 Fragments arranged by size: 5440 546 21 SacII CC_GC'GG Cuts at: 2412 2412 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SanDI GG'GwC_CC Cuts at: 3096 3096 Size: 6007 SapI GCTCTTCn'nnn_ Cuts at: 2249 2249 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2027 2403 3166 3267 3355 4490 5171 Size: 219 982 376 763 101 88 1135 681 Cuts at: 5171 5362 826 Size: 191 1471 Fragments arranged by size: 1471 1135 982 763 681 376 219 191 101 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4111 5012 5274 820 Size: 197 926 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 2762 3931 5 Size: 47 2710 1169 2081 Fragments arranged by size: 2710 2081 1169 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6007 StyI C'CwwG_G Cuts at: 513 1065 2004 3091 3178 3446 513 Size: 552 939 1087 87 268 3074 Fragments arranged by size: 3074 1087 939 552 268 87 SwaI ATTT'AAAT Cuts at: 3213 3213 Size: 6007 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4034 4051 4204 4389 4834 325 Size: 1255 2454 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3195 4253 5965 Size: 274 80 33 51 1443 1218 1058 1712 Cuts at: 5965 96 Size: 138 Fragments arranged by size: 1712 1443 1218 1058 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3099 3131 5967 5999 3099 Size: 32 2836 32 3107 Fragments arranged by size: 3107 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2812 2812 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI