(Circular) (Six-base) MAPSORT of: pFN21AB7675.seq Check: 7143 from: 1 to: 5728 pFN21AB7675 5728 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3791 5175 5443 1632 Size: 2159 1384 268 1917 Fragments arranged by size: 2159 1917 1384 268 AfeI AGC'GCT Cuts at: 2463 2463 Size: 5728 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 4627 5604 1203 Size: 3424 977 1327 Fragments arranged by size: 3424 1327 977 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 2252 2946 1166 Size: 1086 694 3948 Fragments arranged by size: 3948 1086 694 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AlwNI CAG_nnn'CTG Cuts at: 3471 5043 3471 Size: 1572 4156 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 3037 3263 3415 5524 1493 Size: 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2534 3053 1870 Size: 73 289 302 519 4545 Fragments arranged by size: 4545 519 302 289 73 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2521 2539 2776 2924 3049 Size: 325 217 627 734 18 237 148 125 Cuts at: 3049 4504 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 734 627 325 237 217 148 125 18 BanII G_rGCy'C Cuts at: 1391 2198 2369 2749 3047 1391 Size: 807 171 380 298 4072 Fragments arranged by size: 4072 807 380 298 171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2377 2411 3919 3953 1903 Size: 34 440 34 1508 34 3678 Fragments arranged by size: 3678 1508 440 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 2358 2526 2927 3789 4945 1790 Size: 568 168 401 862 1156 2573 Fragments arranged by size: 2573 1156 862 568 401 168 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 3459 4417 Size: 416 53 83 399 24 700 1342 958 Cuts at: 4417 442 Size: 1753 Fragments arranged by size: 1753 1342 958 700 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2212 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2771 4388 1239 Size: 1532 1617 2579 Fragments arranged by size: 2579 1617 1532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3853 4718 5016 3853 Size: 865 298 4565 Fragments arranged by size: 4565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2152 2277 2792 4391 882 Size: 617 653 125 515 1599 2219 Fragments arranged by size: 2219 1599 653 617 515 125 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 5722 5722 Size: 5728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2450 3535 3917 4552 Size: 53 83 186 936 917 1085 382 635 Cuts at: 4552 275 Size: 1451 Fragments arranged by size: 1451 1085 936 917 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 4161 4559 4833 4980 5391 2265 Size: 1896 398 274 147 411 2602 Fragments arranged by size: 2602 1896 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2034 2064 1178 Size: 30 165 30 631 30 4842 Fragments arranged by size: 4842 631 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2055 2575 2687 1186 Size: 869 520 112 4227 Fragments arranged by size: 4227 869 520 112 BseYI C'CCAG_C Cuts at: 2035 2409 2492 2791 2842 2918 4931 5427 2035 Size: 374 83 299 51 76 2013 496 2336 Fragments arranged by size: 2336 2013 496 374 299 83 76 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2120 2514 3178 3939 4967 1540 Size: 275 186 119 394 664 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 394 275 186 119 BsiHKAI G_wGCw'C Cuts at: 2369 3789 3874 4945 2369 Size: 1420 85 1071 3152 Fragments arranged by size: 3152 1420 1071 85 BsmI GAATG_Cn' Cuts at: 2379 2985 3239 3332 2379 Size: 606 254 93 4775 Fragments arranged by size: 4775 606 254 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2183 2183 Size: 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2358 2369 2526 2749 2927 3047 Size: 399 408 160 11 157 223 178 120 Cuts at: 3047 3789 3874 4945 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 223 178 160 157 120 85 11 BspEI T'CCGG_A Cuts at: 2265 5391 2265 Size: 3126 2602 BspHI T'CATG_A Cuts at: 3617 5347 3617 Size: 1730 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3083 844 Size: 2239 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2619 3615 1394 Size: 429 796 996 3507 Fragments arranged by size: 3507 996 796 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4223 4397 65 Size: 1183 2975 174 1396 Fragments arranged by size: 2975 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4372 4559 1700 Size: 110 2562 187 2869 Fragments arranged by size: 2869 2562 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5728 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstYI r'GATC_y Cuts at: 1972 2169 2307 3058 3425 3810 3827 5279 5365 Size: 197 138 751 367 385 17 1452 86 Cuts at: 5365 5377 5723 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 751 385 367 346 197 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2250 513 Size: 552 939 246 3991 Fragments arranged by size: 3991 939 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2946 487 Size: 683 56 1720 3269 Fragments arranged by size: 3269 1720 683 56 BtsI GCAGTG_nn' Cuts at: 3244 4038 4058 3244 Size: 794 20 4914 Fragments arranged by size: 4914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2511 2562 3175 Size: 54 1425 50 275 18 681 51 613 Cuts at: 3175 4064 5693 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 681 613 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2511 3175 1537 Size: 275 699 664 4090 Fragments arranged by size: 4090 699 664 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3658 1353 Size: 2305 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2342 4315 4689 4835 387 Size: 1064 138 753 1973 374 146 1280 Fragments arranged by size: 1973 1280 1064 753 374 146 138 EcoICRI GAG'CTC Cuts at: 2367 2367 Size: 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2771 3791 4388 5175 5443 1239 Size: 393 1139 1020 597 787 268 1524 Fragments arranged by size: 1524 1139 1020 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2753 2753 Size: 5728 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 FspI TGC'GCA Cuts at: 2387 4234 2387 Size: 1847 3881 HaeII r_GCGC'y Cuts at: 1678 2465 4875 1678 Size: 787 2410 2531 Fragments arranged by size: 2531 2410 787 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4369 4401 4443 4475 1373 Size: 32 135 32 2797 32 42 32 2626 Fragments arranged by size: 2797 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3072 3318 669 Size: 988 281 1134 246 3079 Fragments arranged by size: 3079 1134 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2140 2715 4842 5026 1305 Size: 835 575 2127 184 2007 Fragments arranged by size: 2127 2007 835 575 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 10 Size: 54 1425 343 732 3174 Fragments arranged by size: 3174 1425 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2946 3686 4045 4204 518 Size: 648 1086 694 740 359 159 2042 Fragments arranged by size: 2042 1086 740 694 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2065 2795 3144 3821 4969 5214 1606 Size: 429 30 730 349 677 1148 245 2120 Fragments arranged by size: 2120 1148 730 677 429 349 245 30 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 2511 3175 2511 Size: 664 5064 NspI r_CATG'y Cuts at: 1132 1229 2723 2918 3086 4631 1132 Size: 97 1494 195 168 1545 2229 Fragments arranged by size: 2229 1545 1494 195 168 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 4627 4627 Size: 5728 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5728 PfoI T'CCnGG_A Cuts at: 2310 2310 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PstI C_TGCA'G Cuts at: 830 3080 830 Size: 2250 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2035 2795 2035 Size: 760 4968 SacI G_AGCT'C Cuts at: 2369 2369 Size: 5728 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2869 3076 4211 4892 5083 826 Size: 219 1824 207 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 219 207 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 2234 2536 3055 1872 Size: 362 302 519 4545 Fragments arranged by size: 4545 519 362 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 3832 4733 4995 820 Size: 197 926 1889 901 262 1553 Fragments arranged by size: 1889 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 2723 3086 1132 Size: 1591 363 3774 Fragments arranged by size: 3774 1591 363 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 2664 3167 513 Size: 552 939 513 147 503 3074 Fragments arranged by size: 3074 939 552 513 503 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2895 3755 3772 3925 4110 4555 325 Size: 1255 1315 860 17 153 185 445 1498 Fragments arranged by size: 1498 1315 1255 860 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 3974 5686 Size: 274 80 33 51 1443 369 1628 1712 Cuts at: 5686 96 Size: 138 Fragments arranged by size: 1712 1628 1443 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2034 2066 2321 2353 5688 5720 2034 Size: 32 255 32 3335 32 2042 Fragments arranged by size: 3335 2042 255 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5728 XmaI C'CCGG_G Cuts at: 1870 2232 2534 3053 1870 Size: 362 302 519 4545 Fragments arranged by size: 4545 519 362 302 XmnI GAAnn'nnTTC Cuts at: 3857 3857 Size: 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI