(Circular) (Six-base) MAPSORT of: pFN21AB6555.seq Check: 289 from: 1 to: 5821 pFN21AB6555 5821 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2589 2589 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2385 3884 5268 5536 1632 Size: 753 1499 1384 268 1917 Fragments arranged by size: 1917 1499 1384 753 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 2816 1166 Size: 1650 4171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 3564 5136 3564 Size: 1572 4249 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 2306 2319 4375 160 Size: 872 1274 13 2056 1606 Fragments arranged by size: 2056 1606 1274 872 13 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2213 3146 1870 Size: 73 270 933 4545 Fragments arranged by size: 4545 933 270 73 BamHI G'GATC_C Cuts at: 2257 3151 3518 2257 Size: 894 367 4560 Fragments arranged by size: 4560 894 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3142 4597 618 Size: 325 217 627 1355 1455 1842 Fragments arranged by size: 1842 1455 1355 627 325 217 BanII G_rGCy'C Cuts at: 1391 2910 3003 3140 1391 Size: 1519 93 137 4072 Fragments arranged by size: 4072 1519 137 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3051 928 Size: 971 1152 3698 Fragments arranged by size: 3698 1152 971 BbvCI CC'TCA_GC Cuts at: 2186 2436 2186 Size: 250 5571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2931 3882 5038 1790 Size: 1141 951 1156 2573 Fragments arranged by size: 2573 1156 1141 951 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2440 2863 3552 Size: 416 53 83 399 24 1023 423 689 Cuts at: 3552 4510 442 Size: 958 1753 Fragments arranged by size: 1753 1023 958 689 423 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2432 2507 2663 2867 2933 4481 1239 Size: 1010 183 75 156 204 66 1548 2579 Fragments arranged by size: 2579 1548 1010 204 183 156 75 66 Bpu10I CC'TnA_GC Cuts at: 2186 2436 2186 Size: 250 5571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3612 493 Size: 711 2408 2702 Fragments arranged by size: 2702 2408 711 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3628 4010 4645 275 Size: 53 83 186 936 2095 382 635 1451 Fragments arranged by size: 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 2246 1186 Size: 985 75 4761 Fragments arranged by size: 4761 985 75 BseYI C'CCAG_C Cuts at: 5024 5520 5024 Size: 496 5325 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2918 1163 Size: 573 1182 4066 Fragments arranged by size: 4066 1182 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3003 3882 3967 5038 3003 Size: 879 85 1071 3786 Fragments arranged by size: 3786 1071 879 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2910 2931 3003 3140 3882 3967 5038 Size: 399 1120 21 72 137 742 85 1071 Cuts at: 5038 1391 Size: 2174 Fragments arranged by size: 2174 1120 1071 742 399 137 85 72 21 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 2589 3176 844 Size: 1343 402 587 3489 Fragments arranged by size: 3489 1343 587 402 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4465 4652 1700 Size: 110 2655 187 2869 Fragments arranged by size: 2869 2655 187 110 BsrGI T'GTAC_A Cuts at: 96 3087 96 Size: 2991 2830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 794 Size: 1679 4142 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 2440 1184 Size: 1256 4565 BstXI CCAn_nnnn'nTGG Cuts at: 2563 2647 2563 Size: 84 5737 BstYI r'GATC_y Cuts at: 1972 2257 3151 3518 3903 3920 5372 5458 5470 Size: 285 894 367 385 17 1452 86 12 Cuts at: 5470 5816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 894 385 367 346 285 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3035 513 Size: 552 939 1031 3299 Fragments arranged by size: 3299 1031 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2278 3337 4131 4151 2278 Size: 1059 794 20 3948 Fragments arranged by size: 3948 1059 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DraIII CAC_nnn'GTG Cuts at: 2273 2273 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2568 3038 Size: 54 1425 50 275 18 177 561 470 Cuts at: 3038 3268 4157 5786 8 Size: 230 889 1629 43 Fragments arranged by size: 1629 1425 889 561 470 275 230 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3101 3751 1353 Size: 1748 650 3423 Fragments arranged by size: 3423 1748 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3001 3001 Size: 5821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2385 2432 2507 2663 2867 2933 Size: 393 617 136 47 75 156 204 66 Cuts at: 2933 3884 4481 5268 5536 1239 Size: 951 597 787 268 1524 Fragments arranged by size: 1524 951 787 617 597 393 268 204 156 136 75 66 47 EcoO109I rG'GnC_Cy Cuts at: 2109 2109 Size: 5821 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 4968 1678 Size: 3290 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2328 2360 2658 Size: 32 135 32 677 32 47 32 298 Cuts at: 2658 2690 3053 3085 4462 4494 4536 4568 1373 Size: 32 363 32 1377 32 42 32 2626 Fragments arranged by size: 2626 1377 677 363 298 135 47 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3055 3165 3411 669 Size: 988 281 1117 110 246 3079 Fragments arranged by size: 3079 1117 988 281 246 110 HindIII A'AGCT_T Cuts at: 748 2282 2396 748 Size: 1534 114 4173 Fragments arranged by size: 4173 1534 114 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2314 3000 4935 5119 1305 Size: 1009 686 1935 184 2007 Fragments arranged by size: 2007 1935 1009 686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2570 10 Size: 54 1425 343 177 561 3261 Fragments arranged by size: 3261 1425 561 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 2742 2816 3779 4138 4297 518 Size: 648 1479 97 74 963 359 159 2042 Fragments arranged by size: 2042 1479 963 648 359 159 97 74 MspA1I CmG'CkG Cuts at: 1606 3037 3237 3914 5062 5307 1606 Size: 1431 200 677 1148 245 2120 Fragments arranged by size: 2120 1431 1148 677 245 200 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4330 Fragments arranged by size: 4330 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PfoI T'CCnGG_A Cuts at: 2484 2729 2910 2484 Size: 245 181 5395 Fragments arranged by size: 5395 245 181 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2109 2109 Size: 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 2154 2394 2478 3173 830 Size: 1324 240 84 695 3478 Fragments arranged by size: 3478 1324 695 240 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 SacI G_AGCT'C Cuts at: 3003 3003 Size: 5821 SacII CC_GC'GG Cuts at: 3038 3038 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SanDI GG'GwC_CC Cuts at: 2109 2109 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SexAI A'CCwGG_T Cuts at: 2889 2889 Size: 5821 SfcI C'TryA_G Cuts at: 826 1045 2150 2390 2474 3169 4304 4985 5176 Size: 219 1105 240 84 695 1135 681 191 Cuts at: 5176 826 Size: 1471 Fragments arranged by size: 1471 1135 1105 695 681 240 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 3260 513 Size: 552 939 129 1127 3074 Fragments arranged by size: 3074 1127 939 552 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 3848 3865 4018 4203 4648 325 Size: 1255 879 1389 17 153 185 445 1498 Fragments arranged by size: 1498 1389 1255 879 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3087 4067 5779 Size: 274 80 33 51 1443 1110 980 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1712 1443 1110 980 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 3050 1316 Size: 1734 4087 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2563 2876 2563 Size: 313 5508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 2399 3950 2399 Size: 1551 4270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI SwaI