(Circular) (Six-base) MAPSORT of: pFN21AE0534.seq Check: 8141 from: 1 to: 6028 pFN21AE0534 6028 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3238 3238 Size: 6028 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 3371 1937 Size: 487 947 4594 Fragments arranged by size: 4594 947 487 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2605 2875 4091 5475 5743 1632 Size: 973 270 1216 1384 268 1917 Fragments arranged by size: 1917 1384 1216 973 270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 2328 4927 5904 1203 Size: 1125 2599 977 1327 Fragments arranged by size: 2599 1327 1125 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 2027 2109 2134 2514 3230 3771 5343 2027 Size: 82 25 380 716 541 1572 2712 Fragments arranged by size: 2712 1572 716 541 380 82 25 ApaLI G'TGCA_C Cuts at: 4085 5241 4085 Size: 1156 4872 ApoI r'AATT_y Cuts at: 1493 3337 3563 3715 5824 1493 Size: 1844 226 152 2109 1697 Fragments arranged by size: 2109 1844 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 2560 3353 1870 Size: 73 617 793 4545 Fragments arranged by size: 4545 793 617 73 BamHI G'GATC_C Cuts at: 3358 3725 3358 Size: 367 5661 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2532 3029 3349 4804 Size: 325 217 627 333 412 497 320 1455 Cuts at: 4804 618 Size: 1842 Fragments arranged by size: 1842 1455 627 497 412 333 325 320 217 BanII G_rGCy'C Cuts at: 1391 2042 3006 3224 3347 1391 Size: 651 964 218 123 4072 Fragments arranged by size: 4072 964 651 218 123 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 6028 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2551 2585 4219 4253 1903 Size: 34 614 34 1634 34 3678 Fragments arranged by size: 3678 1634 614 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2563 3200 4639 Size: 107 122 71 631 605 891 637 1439 Cuts at: 4639 136 Size: 1525 Fragments arranged by size: 1525 1439 891 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 2520 6023 2520 Size: 3503 2525 BlpI GC'TnA_GC Cuts at: 3223 3445 3223 Size: 222 5806 Bme1580I G_kGCm'C Cuts at: 1790 2012 2537 2559 3032 4089 5245 1790 Size: 222 525 22 473 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 525 473 222 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2212 2821 3035 Size: 416 53 83 399 24 795 609 214 Cuts at: 3035 3759 4717 442 Size: 724 958 1753 Fragments arranged by size: 1753 958 795 724 609 416 399 214 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2021 1056 Size: 965 5063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2545 2775 3133 4688 1239 Size: 1306 230 358 1555 2579 Fragments arranged by size: 2579 1555 1306 358 230 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2930 4153 5018 5316 2930 Size: 1223 865 298 3642 Fragments arranged by size: 3642 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2651 4691 882 Size: 617 1152 2040 2219 Fragments arranged by size: 2219 2040 1152 617 BsaAI yAC'GTr Cuts at: 493 1204 3819 493 Size: 711 2615 2702 Fragments arranged by size: 2702 2615 711 BsaBI GATnn'nnATC Cuts at: 6022 6022 Size: 6028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2360 3835 4217 Size: 53 83 186 936 588 239 1475 382 Cuts at: 4217 4852 275 Size: 635 1451 Fragments arranged by size: 1475 1451 936 635 588 382 239 186 83 53 BsaWI w'CCGG_w Cuts at: 4461 4859 5133 5280 5691 4461 Size: 398 274 147 411 4798 Fragments arranged by size: 4798 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 1178 Size: 30 165 30 910 30 4863 Fragments arranged by size: 4863 910 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 3143 1186 Size: 982 975 4071 Fragments arranged by size: 4071 982 975 BseYI C'CCAG_C Cuts at: 2061 2418 2782 3046 3251 5231 5727 2061 Size: 357 364 264 205 1980 496 2362 Fragments arranged by size: 2362 1980 496 364 357 264 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3216 1163 Size: 573 1480 3975 Fragments arranged by size: 3975 1480 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3018 3478 4239 5267 1540 Size: 275 186 1017 460 761 1028 2301 Fragments arranged by size: 2301 1028 1017 761 460 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 3224 4089 4174 5245 2394 Size: 830 865 85 1071 3177 Fragments arranged by size: 3177 1071 865 830 85 BsmI GAATG_Cn' Cuts at: 3539 3632 3539 Size: 93 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2042 2394 2537 2559 3006 3032 Size: 399 222 30 352 143 22 447 26 Cuts at: 3032 3224 3347 4089 4174 5245 1391 Size: 192 123 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 352 222 192 143 123 85 30 26 22 BspEI T'CCGG_A Cuts at: 5691 5691 Size: 6028 BspHI T'CATG_A Cuts at: 3917 5647 3917 Size: 1730 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3081 3238 3383 844 Size: 2237 157 145 3489 Fragments arranged by size: 3489 2237 157 145 BsrBI CCG'CTC Cuts at: 1394 1823 2203 3915 1394 Size: 429 380 1712 3507 Fragments arranged by size: 3507 1712 429 380 BsrDI GCAATG_nn' Cuts at: 65 1248 4523 4697 65 Size: 1183 3275 174 1396 Fragments arranged by size: 3275 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2280 4672 4859 1700 Size: 110 470 2392 187 2869 Fragments arranged by size: 2869 2392 470 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 2741 4088 5100 2741 Size: 1347 1012 3669 Fragments arranged by size: 3669 1347 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2134 794 Size: 1340 4688 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 2341 3055 1184 Size: 1157 714 4157 Fragments arranged by size: 4157 1157 714 BstXI CCAn_nnnn'nTGG Cuts at: 2653 2653 Size: 6028 BstYI r'GATC_y Cuts at: 1972 2520 3358 3725 4110 4127 5579 5665 5677 Size: 548 838 367 385 17 1452 86 12 Cuts at: 5677 6023 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 838 548 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2316 2316 Size: 6028 BtgI C'CryG_G Cuts at: 513 1065 2464 2538 3012 3237 513 Size: 552 1399 74 474 225 3304 Fragments arranged by size: 3304 1399 552 474 225 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 2683 3243 3544 4338 4358 2683 Size: 560 301 794 20 4353 Fragments arranged by size: 4353 794 560 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5993 DraI TTT'AAA Cuts at: 3332 3332 Size: 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2975 5035 809 Size: 507 1659 2060 1802 Fragments arranged by size: 2060 1802 1659 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2745 3015 Size: 54 1425 50 275 18 465 450 270 Cuts at: 3015 3201 3240 3475 4364 5993 8 Size: 186 39 235 889 1629 43 Fragments arranged by size: 1629 1425 889 465 450 275 270 235 186 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 3015 3475 1537 Size: 275 1203 460 4090 Fragments arranged by size: 4090 1203 460 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2873 3958 1353 Size: 1520 1085 3423 Fragments arranged by size: 3423 1520 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4615 4989 5135 387 Size: 1064 138 3026 374 146 1280 Fragments arranged by size: 3026 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3222 3222 Size: 6028 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2545 2605 2775 2875 3133 4091 4688 Size: 393 913 60 170 100 258 958 597 Cuts at: 4688 5475 5743 1239 Size: 787 268 1524 Fragments arranged by size: 1524 958 913 787 597 393 268 258 170 100 60 EcoO109I rG'GnC_Cy Cuts at: 2206 2502 2511 2565 2665 2737 2760 2770 3139 Size: 296 9 54 100 72 23 10 369 Cuts at: 3139 2206 Size: 5095 Fragments arranged by size: 5095 369 296 100 72 54 23 10 9 EcoRI G'AATT_C Cuts at: 3337 3715 3337 Size: 378 5650 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2650 2650 Size: 6028 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 2124 2177 5175 1678 Size: 446 53 2998 2531 Fragments arranged by size: 2998 2531 446 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2887 2919 4669 Size: 32 135 32 809 32 474 32 1750 Cuts at: 4669 4701 4743 4775 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1750 809 474 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3372 3618 669 Size: 988 281 487 947 246 3079 Fragments arranged by size: 3079 988 947 487 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6028 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KasI G'GCGC_C Cuts at: 2120 2120 Size: 6028 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 3627 3627 Size: 6028 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5142 5326 1305 Size: 3837 184 2007 Fragments arranged by size: 3837 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 10 Size: 54 1425 343 915 3291 Fragments arranged by size: 3291 1425 915 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3274 3986 4345 4504 518 Size: 648 2108 712 359 159 2042 Fragments arranged by size: 2108 2042 712 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2869 3444 4121 5269 5514 1606 Size: 1263 575 677 1148 245 2120 Fragments arranged by size: 2120 1263 1148 677 575 245 NarI GG'CG_CC Cuts at: 2121 2121 Size: 6028 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5476 NdeI CA'TA_TG Cuts at: 387 387 Size: 6028 NheI G'CTAG_C Cuts at: 1052 2017 1052 Size: 965 5063 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 3386 4931 1132 Size: 97 2157 1545 2229 Fragments arranged by size: 2229 2157 1545 97 PasI CC'CwG_GG Cuts at: 1348 2045 1348 Size: 697 5331 PciI A'CATG_T Cuts at: 4927 4927 Size: 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 2109 2488 2907 1108 Size: 1001 379 419 4229 Fragments arranged by size: 4229 1001 419 379 PfoI T'CCnGG_A Cuts at: 3317 3317 Size: 6028 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2347 4097 4129 5610 5642 2315 Size: 32 1750 32 1481 32 2701 Fragments arranged by size: 2701 1750 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2502 2511 2665 2737 2760 2770 2206 Size: 296 9 154 72 23 10 5464 Fragments arranged by size: 5464 296 154 72 23 10 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 2079 3598 2079 Size: 1519 4509 PspXI vC'TCGA_Gb Cuts at: 2560 2560 Size: 6028 PstI C_TGCA'G Cuts at: 830 2130 2142 2874 3380 830 Size: 1300 12 732 506 3478 Fragments arranged by size: 3478 1300 732 506 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 PvuII CAG'CTG Cuts at: 2869 2869 Size: 6028 SacI G_AGCT'C Cuts at: 3224 3224 Size: 6028 SalI G'TCGA_C Cuts at: 1936 2423 3370 1936 Size: 487 947 4594 Fragments arranged by size: 4594 947 487 SanDI GG'GwC_CC Cuts at: 2502 2665 2502 Size: 163 5865 SapI GCTCTTCn'nnn_ Cuts at: 2873 2873 Size: 6028 SbfI CC_TGCA'GG Cuts at: 3380 3380 Size: 6028 ScaI AGT'ACT Cuts at: 4276 5988 4276 Size: 1712 4316 SfcI C'TryA_G Cuts at: 826 1045 2126 2138 2793 2870 3376 4511 5192 Size: 219 1081 12 655 77 506 1135 681 Cuts at: 5192 5383 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1081 681 655 506 219 191 77 12 SfoI GGC'GCC Cuts at: 2122 2122 Size: 6028 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6028 SmaI CCC'GGG Cuts at: 1872 3355 1872 Size: 1483 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2560 2945 4132 5033 5295 820 Size: 197 926 617 385 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 617 385 262 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3386 1132 Size: 2254 3774 SspI AAT'ATT Cuts at: 5 52 3952 5 Size: 47 3900 2081 Fragments arranged by size: 3900 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6028 StyI C'CwwG_G Cuts at: 513 1065 2652 2748 3135 3467 513 Size: 552 1587 96 387 332 3074 Fragments arranged by size: 3074 1587 552 387 332 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2774 3250 3314 4055 4072 4225 4410 Size: 1255 1194 476 64 741 17 153 185 Cuts at: 4410 4855 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1194 741 476 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4274 5986 96 Size: 274 80 33 51 1443 2297 1712 138 Fragments arranged by size: 2297 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2313 2345 5988 6020 2313 Size: 32 3643 32 2321 Fragments arranged by size: 3643 2321 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6028 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XhoI C'TCGA_G Cuts at: 1943 2560 1943 Size: 617 5411 XmaI C'CCGG_G Cuts at: 1870 3353 1870 Size: 1483 4545 XmnI GAAnn'nnTTC Cuts at: 2330 4157 2330 Size: 1827 4201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI