Restriction map for
pFN21AE0371
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbrPI BbvCI
BciVI BclI BfaI BfrI BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KspI MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 270 GGTACC G^GTACC
AccI 1672 GTCTAC GT^[AC][GT]AC
Alw44I 577 GTGCAC G^TGCAC
ApaLI 577 GTGCAC G^TGCAC
Asp718I 270 GGTACC G^GTACC
AspHI 577 GTGCAC G[AT]GC[AT]^C
BamHI 227 GGATCC G^GATCC
BbeI 42 GGCGCC GGCGC^C
BcgI 203 CGATGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 701 AGATCT A^GATCT
BlpI 1294 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1294 GCTGAGC GC^T[ACGT]AGC
BsaAI 1675 TACGTG [CT]AC^GT[GA]
BsiHKAI 577 GTGCAC G[AT]GC[AT]^C
BspHI 1226 TCATGA T^CATGA
BspLU11I 1253 ACATGT A^CATGT
BspMI 1190 ACCTGC ACCTGC(4/8)
BsrDI 1508 GCAATG GCAATG(2/0)
BsrFI 364 ACCGGC [GA]^CCGG[CT]
CelII 1294 GCTGAGC GC^T[ACGT]AGC
Cfr10I 364 ACCGGC [GA]^CCGG[CT]
DraIII 1648 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 652 GACAACCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 42 GGCGCC [GA]GCGC^[CT]
HincII 1609 GTCAAC GT[CT]^[GA]AC
HindII 1609 GTCAAC GT[CT]^[GA]AC
KasI 42 GGCGCC G^GCGCC
KpnI 270 GGTACC GGTAC^C
NarI 42 GGCGCC GG^CGCC
NcoI 1067 CCATGG C^CATGG
PpuMI 1187 AGGACCT [GA]G^G[AT]CC[CT]
PstI 681 CTGCAG CTGCA^G
RcaI 1226 TCATGA T^CATGA
SapI 894 GCTCTTC GCTCTTC(1/4)
SexAI 505 ACCTGGT A^CC[AT]GGT
SfoI 42 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 42 GGCGCC G[GA]^CG[CT]C
1466 GACGCC
AluI 11 AGCT AG^CT
988 AGCT
AlwNI 967 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1186 CAGGACCTG
AvaI 1288 CCCGGG C^[CT]CG[GA]G
1390 CCCGGG
BbsI 355 GTCTTC GAAGAC(2/6)
916 GTCTTC
BglI 1028 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1534 GCCTTCGGGGC
BpmI 375 CTCCAG CTGGAG(16/14)
1666 CTGGAG
BpuAI 355 GTCTTC GAAGAC(2/6)
916 GTCTTC
BsaHI 42 GGCGCC G[GA]^CG[CT]C
1466 GACGCC
BsoBI 1288 CCCGGG C^[CT]CG[GA]G
1390 CCCGGG
BsrBI 942 GAGCGG CCGCTC(-3/-3)
1032 GAGCGG
DdeI 1030 CTGAG C^T[ACGT]AG
1295 CTGAG
DsaI 560 CCGTGG C^C[GA][CT]GG
1067 CCATGG
EagI 557 CGGCCG C^GGCCG
689 CGGCCG
EclXI 557 CGGCCG C^GGCCG
689 CGGCCG
Eco52I 557 CGGCCG C^GGCCG
689 CGGCCG
EcoNI 420 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1662 CCTGCTGGAGG
HgaI 283 GACGC GACGC(5/10)
1466 GACGC
Hin1I 42 GGCGCC G[GA]^CG[CT]C
1466 GACGCC
MspA1I 141 CAGCGG C[AC]G^C[GT]G
1420 CAGCGG
PleI 670 GACTC GAGTC(4/5)
1010 GACTC
SmaI 1288 CCCGGG CCC^GGG
1390 CCCGGG
SphI 645 GCATGC GCATG^C
1643 GCATGC
TaqI 743 TCGA T^CGA
1265 TCGA
TthHB8I 743 TCGA T^CGA
1265 TCGA
XmaCI 1288 CCCGGG C^CCGGG
1390 CCCGGG
XmaI 1288 CCCGGG C^CCGGG
1390 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AflIII AlwI
ApaI AvaII BalI BanI BanII
BbvI BcnI BmyI BsaI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MluNI MnlI MscI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI TspRI
XcmI XhoII