(Circular) (Six-base) MAPSORT of: pFN21AE0371.seq Check: 5056 from: 1 to: 6391 pFN21AE0371 6391 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3678 3734 1937 Size: 1741 56 4594 Fragments arranged by size: 4594 1741 56 Acc65I G'GTAC_C Cuts at: 2275 3712 2275 Size: 1437 4954 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3402 4454 5838 6106 1632 Size: 1770 1052 1384 268 1917 Fragments arranged by size: 1917 1770 1384 1052 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 2187 3258 3369 5290 6267 1203 Size: 984 1071 111 1921 977 1327 Fragments arranged by size: 1921 1327 1071 984 977 111 AgeI A'CCGG_T Cuts at: 5222 5222 Size: 6391 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 2186 1166 Size: 1020 5371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6391 AlwNI CAG_nnn'CTG Cuts at: 2977 3196 4134 5706 2977 Size: 219 938 1572 3662 Fragments arranged by size: 3662 1572 938 219 ApaI G_GGCC'C Cuts at: 2030 2063 3550 2030 Size: 33 1487 4871 Fragments arranged by size: 4871 1487 33 ApaLI G'TGCA_C Cuts at: 2582 4448 5604 2582 Size: 1866 1156 3369 Fragments arranged by size: 3369 1866 1156 ApoI r'AATT_y Cuts at: 1493 3700 3926 4078 6187 1493 Size: 2207 226 152 2109 1697 Fragments arranged by size: 2207 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3293 3395 3716 1870 Size: 73 1350 102 321 4545 Fragments arranged by size: 4545 1350 321 102 73 BamHI G'GATC_C Cuts at: 2232 3721 4088 2232 Size: 1489 367 4535 Fragments arranged by size: 4535 1489 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 2275 3125 3712 5167 Size: 325 217 627 260 228 850 587 1455 Cuts at: 5167 618 Size: 1842 Fragments arranged by size: 1842 1455 850 627 587 325 260 228 217 BanII G_rGCy'C Cuts at: 1391 2030 2042 2063 3210 3330 3550 3710 1391 Size: 639 12 21 1147 120 220 160 4072 Fragments arranged by size: 4072 1147 639 220 160 120 21 12 BbeI G_GCGC'C Cuts at: 2051 2051 Size: 6391 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2353 2914 928 Size: 971 454 561 4405 Fragments arranged by size: 4405 971 561 454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2197 2231 4582 4616 1903 Size: 34 260 34 2351 34 3678 Fragments arranged by size: 3678 2351 260 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4283 5493 4283 Size: 1210 5181 BclI T'GATC_A Cuts at: 1548 1548 Size: 6391 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3039 3545 5002 Size: 107 122 71 631 605 1367 506 1457 Cuts at: 5002 136 Size: 1525 Fragments arranged by size: 1525 1457 1367 631 605 506 122 107 71 BglII A'GATC_T Cuts at: 2706 6386 2706 Size: 3680 2711 BlpI GC'TnA_GC Cuts at: 3300 3808 3300 Size: 508 5883 Bme1580I G_kGCm'C Cuts at: 1790 2030 2063 2586 3185 3550 4452 5608 1790 Size: 240 33 523 599 365 902 1156 2573 Fragments arranged by size: 2573 1156 902 599 523 365 240 33 BmgBI CAC'GTC Cuts at: 1318 2188 2494 3109 1318 Size: 870 306 615 4600 Fragments arranged by size: 4600 870 615 306 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4122 5080 442 Size: 416 53 83 399 24 2705 958 1753 Fragments arranged by size: 2705 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 3692 5051 1239 Size: 1126 1327 1359 2579 Fragments arranged by size: 2579 1359 1327 1126 Bpu10I CC'TnA_GC Cuts at: 3035 3035 Size: 6391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4516 5381 5679 4516 Size: 865 298 5228 Fragments arranged by size: 5228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2476 2525 2858 5054 882 Size: 617 977 49 333 2196 2219 Fragments arranged by size: 2219 2196 977 617 333 49 BsaAI yAC'GTr Cuts at: 493 1204 3682 4182 493 Size: 711 2478 500 2702 Fragments arranged by size: 2702 2478 711 500 BsaBI GATnn'nnATC Cuts at: 6385 6385 Size: 6391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 3472 4198 4580 Size: 53 83 186 936 515 1424 726 382 Cuts at: 4580 5215 275 Size: 635 1451 Fragments arranged by size: 1451 1424 936 726 635 515 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4824 5222 5496 5643 6054 4824 Size: 398 274 147 411 5161 Fragments arranged by size: 5161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3578 3608 1178 Size: 30 165 30 2175 30 3961 Fragments arranged by size: 3961 2175 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2117 2237 2685 1186 Size: 841 90 120 448 4892 Fragments arranged by size: 4892 841 448 120 90 BseYI C'CCAG_C Cuts at: 3240 3410 5594 6090 3240 Size: 170 2184 496 3541 Fragments arranged by size: 3541 2184 496 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2119 2326 2402 1163 Size: 573 383 207 76 5152 Fragments arranged by size: 5152 573 383 207 76 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2565 2667 2697 3841 4602 5630 Size: 275 186 564 102 30 1144 761 1028 Cuts at: 5630 1540 Size: 2301 Fragments arranged by size: 2301 1144 1028 761 564 275 186 102 30 BsiHKAI G_wGCw'C Cuts at: 2586 4452 4537 5608 2586 Size: 1866 85 1071 3369 Fragments arranged by size: 3369 1866 1071 85 BsmI GAATG_Cn' Cuts at: 3902 3995 3902 Size: 93 6298 BsmBI CGTCTCn'nnnn_ Cuts at: 2639 3451 3463 2639 Size: 812 12 5567 Fragments arranged by size: 5567 812 12 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2042 2063 2586 3185 3210 3330 Size: 399 240 12 21 523 599 25 120 Cuts at: 3330 3550 3710 4452 4537 5608 1391 Size: 220 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 599 523 399 240 220 160 120 85 25 21 12 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 3231 4280 6010 3231 Size: 1049 1730 3612 Fragments arranged by size: 3612 1730 1049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3204 3746 844 Size: 2360 542 3489 Fragments arranged by size: 3489 2360 542 BsrBI CCG'CTC Cuts at: 1394 1823 2949 3039 4278 1394 Size: 429 1126 90 1239 3507 Fragments arranged by size: 3507 1239 1126 429 90 BsrDI GCAATG_nn' Cuts at: 65 1248 3520 4886 5060 65 Size: 1183 2272 1366 174 1396 Fragments arranged by size: 2272 1396 1366 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2369 5035 5222 1700 Size: 110 559 2666 187 2869 Fragments arranged by size: 2869 2666 559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 4451 5463 4451 Size: 1012 5379 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6391 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6391 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2557 3670 2545 Size: 12 1113 5266 Fragments arranged by size: 5266 1113 12 BstYI r'GATC_y Cuts at: 1972 2232 2412 2503 2706 3721 4088 4473 4490 Size: 260 180 91 203 1015 367 385 17 Cuts at: 4490 5942 6028 6040 6386 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1015 385 367 346 260 203 180 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2565 3072 513 Size: 552 1500 507 3832 Fragments arranged by size: 3832 1500 552 507 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2222 487 Size: 683 56 996 4656 Fragments arranged by size: 4656 996 683 56 BtsI GCAGTG_nn' Cuts at: 2202 3224 3441 3907 4701 4721 2202 Size: 1022 217 466 794 20 3872 Fragments arranged by size: 3872 1022 794 466 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DraIII CAC_nnn'GTG Cuts at: 3658 3658 Size: 6391 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2663 5398 809 Size: 507 1347 2735 1802 Fragments arranged by size: 2735 1802 1347 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2169 2547 Size: 54 1425 50 275 18 256 83 378 Cuts at: 2547 2562 2694 2824 3438 3838 4727 6356 8 Size: 15 132 130 614 400 889 1629 43 Fragments arranged by size: 1629 1425 889 614 400 378 275 256 132 130 83 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2562 2694 3838 1537 Size: 275 750 132 1144 4090 Fragments arranged by size: 4090 1144 750 275 132 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 2765 2861 2906 4321 1353 Size: 890 522 96 45 1415 3423 Fragments arranged by size: 3423 1415 890 522 96 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2166 3367 4978 5352 5498 387 Size: 1064 138 577 1201 1611 374 146 1280 Fragments arranged by size: 1611 1280 1201 1064 577 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2365 3402 3692 4454 5051 5838 6106 Size: 393 733 1037 290 762 597 787 268 Cuts at: 6106 1239 Size: 1524 Fragments arranged by size: 1524 1037 787 762 733 597 393 290 268 EcoNI CCTnn'n_nnAGG Cuts at: 2429 3671 2429 Size: 1242 5149 EcoO109I rG'GnC_Cy Cuts at: 2026 2027 2060 2974 3193 3546 2026 Size: 1 33 914 219 353 4871 Fragments arranged by size: 4871 914 353 219 33 1 EcoRI G'AATT_C Cuts at: 3700 4078 3700 Size: 378 6013 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 2051 5538 1678 Size: 373 3487 2531 Fragments arranged by size: 3487 2531 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 5032 5064 5106 Size: 32 135 32 812 32 2616 32 42 Cuts at: 5106 5138 1373 Size: 32 2626 Fragments arranged by size: 2626 2616 812 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3616 3735 3981 669 Size: 988 281 1678 119 246 3079 Fragments arranged by size: 3079 1678 988 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 6391 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KasI G'GCGC_C Cuts at: 2047 2047 Size: 6391 KpnI G_GTAC'C Cuts at: 2279 3716 2279 Size: 1437 4954 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2647 5505 5689 1305 Size: 1342 2858 184 2007 Fragments arranged by size: 2858 2007 1342 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 2826 3440 10 Size: 54 1425 343 717 277 614 2961 Fragments arranged by size: 2961 1425 717 614 343 277 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2207 2555 4349 4708 4867 518 Size: 648 1020 21 348 1794 359 159 2042 Fragments arranged by size: 2042 1794 1020 648 359 348 159 21 MspA1I CmG'CkG Cuts at: 1606 2148 3427 3807 4484 5632 5877 1606 Size: 542 1279 380 677 1148 245 2120 Fragments arranged by size: 2120 1279 1148 677 542 380 245 NarI GG'CG_CC Cuts at: 2048 2048 Size: 6391 NcoI C'CATG_G Cuts at: 513 1065 3072 513 Size: 552 2007 3832 Fragments arranged by size: 3832 2007 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 2654 3262 3652 3749 5294 1132 Size: 97 1425 608 390 97 1545 2229 Fragments arranged by size: 2229 1545 1425 608 390 97 97 PasI CC'CwG_GG Cuts at: 1348 2023 2064 3133 1348 Size: 675 41 1069 4606 Fragments arranged by size: 4606 1069 675 41 PciI A'CATG_T Cuts at: 3258 5290 3258 Size: 2032 4359 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6391 PfoI T'CCnGG_A Cuts at: 2859 2859 Size: 6391 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4460 4492 5973 6005 4460 Size: 32 1481 32 4846 Fragments arranged by size: 4846 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3193 3193 Size: 6391 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PspOMI G'GGCC_C Cuts at: 2026 2059 3546 2026 Size: 33 1487 4871 Fragments arranged by size: 4871 1487 33 PstI C_TGCA'G Cuts at: 830 2690 3743 830 Size: 1860 1053 3478 Fragments arranged by size: 3478 1860 1053 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SapI GCTCTTCn'nnn_ Cuts at: 2906 2906 Size: 6391 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SexAI A'CCwGG_T Cuts at: 2510 2510 Size: 6391 SfcI C'TryA_G Cuts at: 826 1045 2599 2686 2989 3739 4874 5555 5746 Size: 219 1554 87 303 750 1135 681 191 Cuts at: 5746 826 Size: 1471 Fragments arranged by size: 1554 1471 1135 750 681 303 219 191 87 SfoI GGC'GCC Cuts at: 2049 2049 Size: 6391 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3295 3397 3718 1872 Size: 1423 102 321 4545 Fragments arranged by size: 4545 1423 321 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 4495 5396 5658 820 Size: 197 926 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 2654 3652 3749 1132 Size: 1522 998 97 3774 Fragments arranged by size: 3774 1522 998 97 SspI AAT'ATT Cuts at: 5 52 4315 5 Size: 47 4263 2081 Fragments arranged by size: 4263 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2031 3024 3072 3321 3830 513 Size: 552 966 993 48 249 509 3074 Fragments arranged by size: 3074 993 966 552 509 249 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 3233 3403 4418 4435 4588 4773 Size: 1255 1533 120 170 1015 17 153 185 Cuts at: 4773 5218 325 Size: 445 1498 Fragments arranged by size: 1533 1498 1255 1015 445 185 170 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2751 4637 6349 Size: 274 80 33 51 1443 774 1886 1712 Cuts at: 6349 96 Size: 138 Fragments arranged by size: 1886 1712 1443 774 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2068 2100 3359 3391 3644 3676 6351 6383 2068 Size: 32 1259 32 253 32 2675 32 2076 Fragments arranged by size: 2675 2076 1259 253 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6391 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2974 3100 3169 3397 2974 Size: 126 69 228 5968 Fragments arranged by size: 5968 228 126 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3293 3395 3716 1870 Size: 1423 102 321 4545 Fragments arranged by size: 4545 1423 321 102 XmnI GAAnn'nnTTC Cuts at: 4520 4520 Size: 6391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI