################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NarI NciI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AclI 220 AACGTT AA^CGTT AcsI 25 GAATTT [GA]^AATT[CT] AflIII 203 ACGTGT A^C[GA][CT]GT AhdI 45 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 568 GTGCAC G^TGCAC AlwI 535 GATCC GGATC(4/5) ApaLI 568 GTGCAC G^TGCAC ApoI 25 GAATTT [GA]^AATT[CT] Asp700I 699 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 45 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 568 GTGCAC G[AT]GC[AT]^C BbrPI 319 CACGTG CAC^GTG BbvCI 151 CCTCAGC CCTCAGC(-5/-2) BbvI 371 GCTGC GCAGC(8/12) BmyI 568 GTGCAC G[AGT]GC[ACT]^C BsaAI 319 CACGTG [CT]AC^GT[GA] BsaBI 138 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 777 GAGACC GGTCTC(1/5) BsaJI 36 CCTGGG C^C[ACGT][ACGT]GG BseRI 213 CTCCTC GAGGAG(10/8) BsiHKAI 568 GTGCAC G[AT]GC[AT]^C Bsp1286I 568 GTGCAC G[AGT]GC[ACT]^C BsrDI 306 CATTGC GCAATG(2/0) BsrFI 367 GCCGGC [GA]^CCGG[CT] BstXI 230 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 534 AGATCC [GA]^GATC[CT] CfoI 678 GCGC GCG^C Cfr10I 367 GCCGGC [GA]^CCGG[CT] EaeI 365 TGGCCG [CT]^GGCC[GA] Eam1105I 45 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 350 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 371 GCTGC GC^[ACGT]GC HapII 368 CCGG C^CGG HhaI 678 GCGC GCG^C HinP1I 678 GCGC G^CGC HincII 895 GTCAAC GT[CT]^[GA]AC HindII 895 GTCAAC GT[CT]^[GA]AC HpaII 368 CCGG C^CGG HphI 908 TCACC GGTGA(8/7) ItaI 371 GCTGC GC^[ACGT]GC MamI 138 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 761 TCTTC GAAGA(8/7) MflI 534 AGATCC [GA]^GATC[CT] MseI 608 TTAA T^TAA MspI 368 CCGG C^CGG NaeI 367 GCCGGC GCC^GGC NdeI 917 CATATG CA^TATG NgoMIV 367 GCCGGC G^CCGGC NspI 674 GCATGC [GA]CATG^[CT] PmaCI 319 CACGTG CAC^GTG PmlI 319 CACGTG CAC^GTG Psp1406I 220 AACGTT AA^CGTT SphI 674 GCATGC GCATG^C SspI 118 AATATT AAT^ATT Tru9I 608 TTAA T^TAA TseI 371 GCTGC G^C[AT]GC Tsp45I 19 GTGAC ^GT[GC]AC XhoII 534 AGATCC [GA]^GATC[CT] XmnI 699 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 91 GCGG CCGC(-3/-1) 199 GCGG AfaI 171 GTAC GT^AC 360 GTAC BclI 101 TGATCA T^GATCA 128 TGATCA BfaI 88 CTAG C^TAG 404 CTAG BsgI 289 GTGCAG GTGCAG(16/14) 530 GTGCAG BsmAI 256 GAGAC GTCTC(1/5) 777 GAGAC BssKI 36 CCTGG ^CC[ACGT]GG 336 CCTGG BstNI 36 CCTGG CC^[AT]GG 336 CCTGG Cac8I 367 GCCGGC GC[ACGT]^[ACGT]GC 674 GCATGC DraII 718 GGGTCCC [GA]G^G[ACGT]CC[CT] 751 GGGACCC EcoO109I 718 GGGTCCC [GA]G^G[ACGT]CC[CT] 751 GGGACCC EcoRII 36 CCTGG ^CC[AT]GG 336 CCTGG FbaI 101 TGATCA T^GATCA 128 TGATCA HaeIII 348 GGCC GG^CC 366 GGCC MaeI 88 CTAG C^TAG 404 CTAG MaeIII 19 GTGAC ^GT[ACGT]AC 223 GTTAC MvaI 36 CCTGG CC^[AT]GG 336 CCTGG PleI 162 GAGTC GAGTC(4/5) 183 GAGTC PpuMI 718 GGGTCCC [GA]G^G[AT]CC[CT] 751 GGGACCC RsaI 171 GTAC GT^AC 360 GTAC ScrFI 36 CCTGG CC^[ACGT]GG 336 CCTGG SfaNI 281 GATGC GCATC(5/9) 143 GCATC SfcI 61 CTACAG C^T[GA][CT]AG 861 CTACAG TspRI 292 CAGTG CA[GC]TG[ACGT][ACGT]^ 783 CACTG XcmI 266 CCATTTCCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 549 CCAGATTGCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI AvaII BsiYI BslI BsmFI BstF5I Cfr13I DdeI DpnII FokI HinfI MaeII MboI MnlI MslI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I TfiI Tsp509I