(Circular) (Six-base) MAPSORT of: pFN21AB7134.seq Check: 9939 from: 1 to: 5665 pFN21AB7134 5665 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 5112 5380 1632 Size: 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 4564 5541 1203 Size: 3361 977 1327 Fragments arranged by size: 3361 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 2036 2736 1166 Size: 870 700 4095 Fragments arranged by size: 4095 870 700 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2621 2653 2621 Size: 32 5633 AlwNI CAG_nnn'CTG Cuts at: 3408 4980 3408 Size: 1572 4093 ApaLI G'TGCA_C Cuts at: 3722 4878 3722 Size: 1156 4509 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2268 2990 1870 Size: 73 325 722 4545 Fragments arranged by size: 4545 722 325 73 BamHI G'GATC_C Cuts at: 2456 2995 3362 2456 Size: 539 367 4759 Fragments arranged by size: 4759 539 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2879 2986 4441 618 Size: 325 217 627 1092 107 1455 1842 Fragments arranged by size: 1842 1455 1092 627 325 217 107 BanII G_rGCy'C Cuts at: 1391 2984 1391 Size: 1593 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2131 928 Size: 971 232 4462 Fragments arranged by size: 4462 971 232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2060 3557 4767 2060 Size: 1497 1210 2958 Fragments arranged by size: 2958 1497 1210 BclI T'GATC_A Cuts at: 1548 2487 1548 Size: 939 4726 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 3726 4882 1790 Size: 1936 1156 2573 Fragments arranged by size: 2573 1936 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4325 1239 Size: 3086 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4655 4953 3790 Size: 865 298 4502 Fragments arranged by size: 4502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 2435 5659 2435 Size: 3224 2441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2949 2979 1178 Size: 30 165 30 1546 30 3864 Fragments arranged by size: 3864 1546 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 4868 5364 4868 Size: 496 5169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 1163 Size: 573 414 4678 Fragments arranged by size: 4678 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 3726 3811 4882 2409 Size: 1317 85 1071 3192 Fragments arranged by size: 3192 1317 1071 85 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2984 3726 3811 4882 1391 Size: 399 619 575 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 619 575 399 85 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2850 3020 844 Size: 2006 170 3489 Fragments arranged by size: 3489 2006 170 BsrBI CCG'CTC Cuts at: 1394 1823 2701 3552 1394 Size: 429 878 851 3507 Fragments arranged by size: 3507 878 851 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2121 4160 4334 65 Size: 1183 873 2039 174 1396 Fragments arranged by size: 2039 1396 1183 873 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 2782 96 Size: 2686 2979 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5665 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstXI CCAn_nnnn'nTGG Cuts at: 2212 2347 2614 2212 Size: 135 267 5263 Fragments arranged by size: 5263 267 135 BstYI r'GATC_y Cuts at: 1972 2241 2456 2995 3362 3747 3764 5216 5302 Size: 269 215 539 367 385 17 1452 86 Cuts at: 5302 5314 5660 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 539 385 367 346 269 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2352 2452 513 Size: 552 1287 100 3726 Fragments arranged by size: 3726 1287 552 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2387 2409 2474 3181 3975 3995 2387 Size: 22 65 707 794 20 4057 Fragments arranged by size: 4057 794 707 65 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2373 3112 Size: 54 1425 50 275 18 519 24 739 Cuts at: 3112 4001 5630 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 739 519 275 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3595 1353 Size: 2242 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3728 4325 5112 5380 1239 Size: 393 2096 597 787 268 1524 Fragments arranged by size: 2096 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 4812 1678 Size: 3134 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373 Size: 32 135 32 2734 32 42 32 2626 Fragments arranged by size: 2734 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 2375 10 Size: 54 1425 343 519 24 3300 Fragments arranged by size: 3300 1425 519 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2514 2547 2624 2736 2738 3623 Size: 648 870 478 33 77 112 2 885 Cuts at: 3623 3982 4141 518 Size: 359 159 2042 Fragments arranged by size: 2042 885 870 648 478 359 159 112 77 33 2 MspA1I CmG'CkG Cuts at: 1606 2454 2931 3081 3758 4906 5151 1606 Size: 848 477 150 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 677 477 245 150 NcoI C'CATG_G Cuts at: 513 1065 2352 513 Size: 552 1287 3826 Fragments arranged by size: 3826 1287 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2030 2197 3023 4568 1132 Size: 97 801 167 826 1545 2229 Fragments arranged by size: 2229 1545 826 801 167 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 2768 1108 Size: 1660 4005 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 2404 3017 830 Size: 1574 613 3478 Fragments arranged by size: 3478 1574 613 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 PvuII CAG'CTG Cuts at: 2931 2931 Size: 5665 SacII CC_GC'GG Cuts at: 2455 2455 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SfcI C'TryA_G Cuts at: 826 1045 2400 3013 4148 4829 5020 826 Size: 219 1355 613 1135 681 191 1471 Fragments arranged by size: 1471 1355 1135 681 613 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2992 1872 Size: 1120 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3769 4670 4932 820 Size: 197 926 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 2197 3023 1132 Size: 1065 826 3774 Fragments arranged by size: 3774 1065 826 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2288 2711 1517 Size: 771 423 4471 Fragments arranged by size: 4471 771 423 StyI C'CwwG_G Cuts at: 513 1065 2352 2706 3104 513 Size: 552 1287 354 398 3074 Fragments arranged by size: 3074 1287 552 398 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2400 2819 3692 3709 3862 4047 4492 Size: 1255 820 419 873 17 153 185 445 Cuts at: 4492 325 Size: 1498 Fragments arranged by size: 1498 1255 873 820 445 419 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2782 3911 5623 Size: 274 80 33 51 1443 805 1129 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1712 1443 1129 805 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2709 2741 5625 5657 2709 Size: 32 2884 32 2717 Fragments arranged by size: 2884 2717 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2078 2107 2344 2078 Size: 29 237 5399 Fragments arranged by size: 5399 237 29 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2990 1870 Size: 1120 4545 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI