(Circular) (Six-base) MAPSORT of: pFN21AB7211.seq Check: 6000 from: 1 to: 5644 pFN21AB7211 5644 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2902 3707 5091 5359 1632 Size: 1270 805 1384 268 1917 Fragments arranged by size: 1917 1384 1270 805 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2723 4543 5520 1203 Size: 1520 1820 977 1327 Fragments arranged by size: 1820 1520 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2703 2974 3341 2703 Size: 271 367 5006 Fragments arranged by size: 5006 367 271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 4420 618 Size: 325 217 627 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2424 2963 1391 Size: 621 412 539 4072 Fragments arranged by size: 4072 621 539 412 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2422 2527 928 Size: 971 523 105 4045 Fragments arranged by size: 4045 971 523 105 BbvCI CC'TCA_GC Cuts at: 2192 2192 Size: 5644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2654 3536 4746 2654 Size: 882 1210 3552 Fragments arranged by size: 3552 1210 882 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BfrBI ATG'CAT Cuts at: 2295 2295 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 3705 4861 1790 Size: 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4304 1239 Size: 3065 2579 Bpu10I CC'TnA_GC Cuts at: 2192 2192 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 3769 4634 4932 2543 Size: 1226 865 298 3255 Fragments arranged by size: 3255 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 2122 4847 5343 2122 Size: 2725 496 2423 Fragments arranged by size: 2725 2423 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 3705 3790 4861 2424 Size: 1281 85 1071 3207 Fragments arranged by size: 3207 1281 1071 85 BsmI GAATG_Cn' Cuts at: 2297 3155 3248 2297 Size: 858 93 4693 Fragments arranged by size: 4693 858 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2424 2963 3705 3790 4861 1391 Size: 399 222 412 539 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 539 412 399 222 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 2409 3533 5263 2409 Size: 1124 1730 2790 Fragments arranged by size: 2790 1730 1124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2999 844 Size: 1295 860 3489 Fragments arranged by size: 3489 1295 860 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 2835 96 Size: 2739 2905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2122 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2703 2974 3341 3726 3743 5195 5281 5293 Size: 731 271 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 731 385 367 346 271 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2681 2879 2681 Size: 198 5446 BtgI C'CryG_G Cuts at: 513 1065 2115 513 Size: 552 1050 4042 Fragments arranged by size: 4042 1050 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 2276 1554 Size: 722 4922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2500 2816 4651 809 Size: 507 1184 316 1835 1802 Fragments arranged by size: 1835 1802 1184 507 316 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2773 3091 Size: 54 1425 50 275 18 233 710 318 Cuts at: 3091 3980 5609 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 710 318 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2412 2921 3574 1353 Size: 1059 509 653 3423 Fragments arranged by size: 3423 1059 653 509 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2422 2422 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2902 3707 4304 5091 5359 1239 Size: 393 1270 805 597 787 268 1524 Fragments arranged by size: 1524 1270 805 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2285 Size: 5644 EcoO109I rG'GnC_Cy Cuts at: 2686 2902 2686 Size: 216 5428 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 EcoRV GAT'ATC Cuts at: 2578 2578 Size: 5644 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2921 2921 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2775 10 Size: 54 1425 343 943 2879 Fragments arranged by size: 2879 1425 943 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2638 2852 3602 3961 4120 518 Size: 648 1011 461 214 750 359 159 2042 Fragments arranged by size: 2042 1011 750 648 461 359 214 159 MspA1I CmG'CkG Cuts at: 1606 2155 3060 3737 4885 5130 1606 Size: 549 905 677 1148 245 2120 Fragments arranged by size: 2120 1148 905 677 549 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5092 NdeI CA'TA_TG Cuts at: 387 2362 2381 2656 387 Size: 1975 19 275 3375 Fragments arranged by size: 3375 1975 275 19 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NsiI A_TGCA'T Cuts at: 2297 2297 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2107 2260 2727 3002 4547 1132 Size: 97 878 153 467 275 1545 2229 Fragments arranged by size: 2229 1545 878 467 275 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 2723 4543 2723 Size: 1820 3824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2686 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2155 2155 Size: 5644 SacI G_AGCT'C Cuts at: 2424 2424 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SanDI GG'GwC_CC Cuts at: 2686 2686 Size: 5644 SapI GCTCTTCn'nnn_ Cuts at: 2412 2412 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2872 2992 4127 4808 4999 826 Size: 219 1827 120 1135 681 191 1471 Fragments arranged by size: 1827 1471 1135 681 219 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 3748 4649 4911 820 Size: 197 926 615 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 615 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 2769 3568 5 Size: 47 2717 799 2081 Fragments arranged by size: 2717 2081 799 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2905 3083 513 Size: 552 1840 178 3074 Fragments arranged by size: 3074 1840 552 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2382 2866 3671 3688 3841 4026 4471 Size: 1255 802 484 805 17 153 185 445 Cuts at: 4471 325 Size: 1498 Fragments arranged by size: 1498 1255 805 802 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2268 2835 Size: 274 80 33 51 1443 204 87 567 Cuts at: 2835 3890 5602 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 567 274 204 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2915 3773 2915 Size: 858 4786 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI