Restriction map for
pFN21AE0147
(Linear) (Six-base) MAPSORT of: KIEE0147.seq Check: 382 from: 1 to: 612
KIEE0147.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AfeI AGC'GCT
Cuts at: 0 362 612
Size: 362 250
AhdI GACnn_n'nnGTC
Cuts at: 0 145 612
Size: 145 467
AlwNI CAG_nnn'CTG
Cuts at: 0 115 333 612
Size: 115 218 279
Fragments arranged by size:
279 218 115
AvaI C'yCGr_G
Cuts at: 0 201 612
Size: 201 411
BanI G'GyrC_C
Cuts at: 0 337 612
Size: 337 275
BanII G_rGCy'C
Cuts at: 0 449 612
Size: 449 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 474 612
Size: 474 138
BglI GCCn_nnn'nGGC
Cuts at: 0 353 612
Size: 353 259
BlpI GC'TnA_GC
Cuts at: 0 424 612
Size: 424 188
Bme1580I G_kGCm'C
Cuts at: 0 157 260 340 612
Size: 157 103 80 272
Fragments arranged by size:
272 157 103 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 403 612
Size: 403 209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 398 612
Size: 289 109 214
Fragments arranged by size:
289 214 109
BsaAI yAC'GTr
Cuts at: 0 474 612
Size: 474 138
BsaWI w'CCGG_w
Cuts at: 0 144 612
Size: 144 468
BseYI C'CCAG_C
Cuts at: 0 43 222 612
Size: 43 179 390
Fragments arranged by size:
390 179 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 128 604 612
Size: 70 58 476 8
Fragments arranged by size:
476 70 58 8
Bsp1286I G_dGCh'C
Cuts at: 0 157 260 340 449 612
Size: 157 103 80 109 163
Fragments arranged by size:
163 157 109 103 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 612
Size: 92 520
BsrBI CCG'CTC
Cuts at: 0 77 612
Size: 77 535
BsrDI GCAATG_nn'
Cuts at: 0 531 612
Size: 531 81
BsrFI r'CCGG_y
Cuts at: 0 161 612
Size: 161 451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 115 612
Size: 115 497
Bsu36I CC'TnA_GG
Cuts at: 0 134 612
Size: 134 478
BtgI C'CryG_G
Cuts at: 0 36 612
Size: 36 576
BtsI GCAGTG_nn'
Cuts at: 0 113 260 612
Size: 113 147 352
Fragments arranged by size:
352 147 113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 513 548 612
Size: 513 35 64
Fragments arranged by size:
513 64 35
EaeI y'GGCC_r
Cuts at: 0 534 612
Size: 534 78
EarI CTCTTCn'nnn_
Cuts at: 0 97 406 612
Size: 97 309 206
Fragments arranged by size:
309 206 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 398 612
Size: 289 109 214
Fragments arranged by size:
289 214 109
EcoO109I rG'GnC_Cy
Cuts at: 0 139 206 330 612
Size: 139 67 124 282
Fragments arranged by size:
282 139 124 67
HaeII r_GCGC'y
Cuts at: 0 364 612
Size: 364 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 131 163 612
Size: 32 99 32 449
Fragments arranged by size:
449 99 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 281 612
Size: 163 118 331
Fragments arranged by size:
331 163 118
MscI TGG'CCA
Cuts at: 0 536 612
Size: 536 76
MspA1I CmG'CkG
Cuts at: 0 29 38 309 376 612
Size: 29 9 271 67 236
Fragments arranged by size:
271 236 67 29 9
NaeI GCC'GGC
Cuts at: 0 163 612
Size: 163 449
NgoMIV G'CCGG_C
Cuts at: 0 161 612
Size: 161 451
PasI CC'CwG_GG
Cuts at: 0 333 612
Size: 333 279
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 612
Size: 333 279
PmlI CAC'GTG
Cuts at: 0 474 612
Size: 474 138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 612
Size: 274 32 306
Fragments arranged by size:
306 274 32
PpuMI rG'GwC_Cy
Cuts at: 0 139 206 612
Size: 139 67 406
Fragments arranged by size:
406 139 67
PstI C_TGCA'G
Cuts at: 0 28 34 612
Size: 28 6 578
Fragments arranged by size:
578 28 6
PvuII CAG'CTG
Cuts at: 0 29 376 612
Size: 29 347 236
Fragments arranged by size:
347 236 29
SacII CC_GC'GG
Cuts at: 0 39 612
Size: 39 573
SanDI GG'GwC_CC
Cuts at: 0 206 612
Size: 206 406
SfcI C'TryA_G
Cuts at: 0 24 30 612
Size: 24 6 582
Fragments arranged by size:
582 24 6
StyI C'CwwG_G
Cuts at: 0 437 449 612
Size: 437 12 163
Fragments arranged by size:
437 163 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 528 612
Size: 214 314 84
Fragments arranged by size:
314 214 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 503 535 612
Size: 46 32 425 32 77
Fragments arranged by size:
425 77 46 32 32
Enzymes that do cut:
AfeI AhdI AlwNI AvaI BanI BanII BbsI BglI
BlpI Bme1580I BplI BpmI BsaAI BsaWI BseYI BsgI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI Bsu36I BtgI
BtsI CspCI EaeI EarI Eco57MI EcoO109I HaeII Hin4I
MmeI MscI MspA1I NaeI NgoMIV PasI PflMI PmlI
PpiI PpuMI PstI PvuII SacII SanDI SfcI StyI
TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BseRI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI