(Circular) (Six-base) MAPSORT of: pFN21AB8905.seq Check: 2360 from: 1 to: 5416 pFN21AB8905 5416 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3226 278 Size: 53 83 186 936 1690 2468 Fragments arranged by size: 2468 1690 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2759 1937 Size: 822 4594 Acc65I G'GTAC_C Cuts at: 2737 2737 Size: 5416 AclI AA'CG_TT Cuts at: 1563 3543 3916 1563 Size: 1980 373 3063 Fragments arranged by size: 3063 1980 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2301 2504 2525 3479 4863 5131 1632 Size: 514 155 203 21 954 1384 268 1917 Fragments arranged by size: 1917 1384 954 514 268 203 155 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5219 AflIII A'CryG_T Cuts at: 1203 4315 5292 1203 Size: 3112 977 1327 Fragments arranged by size: 3112 1327 977 AgeI A'CCGG_T Cuts at: 4247 4247 Size: 5416 AhdI GACnn_n'nnGTC Cuts at: 4145 4145 Size: 5416 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5416 AlwNI CAG_nnn'CTG Cuts at: 2248 2321 3159 4731 2248 Size: 73 838 1572 2933 Fragments arranged by size: 2933 1572 838 73 ApaI G_GGCC'C Cuts at: 2315 2334 2315 Size: 19 5397 ApaLI G'TGCA_C Cuts at: 3473 4629 3473 Size: 1156 4260 ApoI r'AATT_y Cuts at: 1493 2725 2951 3103 5212 1493 Size: 1232 226 152 2109 1697 Fragments arranged by size: 2109 1697 1232 226 152 AseI AT'TA_AT Cuts at: 160 1032 3970 160 Size: 872 2938 1606 Fragments arranged by size: 2938 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5416 AvaI C'yCGr_G Cuts at: 1870 1943 2631 2674 2684 2741 1870 Size: 73 688 43 10 57 4545 Fragments arranged by size: 4545 688 73 57 43 10 BamHI G'GATC_C Cuts at: 2746 3113 2746 Size: 367 5049 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 2493 2737 4192 618 Size: 325 217 627 408 298 244 1455 1842 Fragments arranged by size: 1842 1455 627 408 325 298 244 217 BanII G_rGCy'C Cuts at: 1391 2126 2315 2334 2357 2374 2735 1391 Size: 735 189 19 23 17 361 4072 Fragments arranged by size: 4072 735 361 189 23 19 17 BbeI G_GCGC'C Cuts at: 2497 2497 Size: 5416 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2410 2444 2513 2547 3607 3641 1903 Size: 34 473 34 69 34 1060 34 3678 Fragments arranged by size: 3678 1060 473 69 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3308 4518 3308 Size: 1210 4206 BclI T'GATC_A Cuts at: 1548 1548 Size: 5416 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 2585 2618 Size: 107 122 71 631 605 428 485 33 Cuts at: 2618 2627 4027 136 Size: 9 1400 1525 Fragments arranged by size: 1525 1400 631 605 485 428 122 107 71 33 9 BglII A'GATC_T Cuts at: 5411 5411 Size: 5416 BlpI GC'TnA_GC Cuts at: 2833 2833 Size: 5416 Bme1580I G_kGCm'C Cuts at: 1790 2198 2315 2334 3477 4633 1790 Size: 408 117 19 1143 1156 2573 Fragments arranged by size: 2573 1156 1143 408 117 19 BmgBI CAC'GTC Cuts at: 1318 2380 1318 Size: 1062 4354 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 2309 3147 Size: 416 53 83 399 24 712 180 838 Cuts at: 3147 4105 442 Size: 958 1753 Fragments arranged by size: 1753 958 838 712 416 399 180 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2585 4076 1239 Size: 992 354 1491 2579 Fragments arranged by size: 2579 1491 992 354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3541 4406 4704 3541 Size: 865 298 4253 Fragments arranged by size: 4253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2422 4079 882 Size: 617 923 1657 2219 Fragments arranged by size: 2219 1657 923 617 BsaAI yAC'GTr Cuts at: 493 1204 3207 493 Size: 711 2003 2702 Fragments arranged by size: 2702 2003 711 BsaBI GATnn'nnATC Cuts at: 5410 5410 Size: 5416 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2494 3223 3605 4240 Size: 53 83 186 936 961 729 382 635 Cuts at: 4240 275 Size: 1451 Fragments arranged by size: 1451 961 936 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3849 4247 4521 4668 5079 3849 Size: 398 274 147 411 4186 Fragments arranged by size: 4186 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 4360 Fragments arranged by size: 4360 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2375 1186 Size: 1042 147 4227 Fragments arranged by size: 4227 1042 147 BseYI C'CCAG_C Cuts at: 2018 2248 2255 2373 2592 4619 5115 2018 Size: 230 7 118 219 2027 496 2319 Fragments arranged by size: 2319 2027 496 230 219 118 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4843 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2866 3627 4655 1540 Size: 275 186 865 761 1028 2301 Fragments arranged by size: 2301 1028 865 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3477 3562 4633 3477 Size: 85 1071 4260 Fragments arranged by size: 4260 1071 85 BsmI GAATG_Cn' Cuts at: 2927 3020 2927 Size: 93 5323 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2198 2315 2334 2357 2374 2735 Size: 399 336 72 117 19 23 17 361 Cuts at: 2735 3477 3562 4633 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 336 117 85 72 23 19 17 BspEI T'CCGG_A Cuts at: 5079 5079 Size: 5416 BspHI T'CATG_A Cuts at: 3305 5035 3305 Size: 1730 3686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2771 844 Size: 1927 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2395 2679 3303 1394 Size: 429 572 284 624 3507 Fragments arranged by size: 3507 624 572 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 3911 4085 65 Size: 1183 2663 174 1396 Fragments arranged by size: 2663 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2442 2664 4060 4247 1700 Size: 110 632 222 1396 187 2869 Fragments arranged by size: 2869 1396 632 222 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5416 BssHII G'CGCG_C Cuts at: 1925 2575 1925 Size: 650 4766 BssSI C'ACGA_G Cuts at: 3476 4488 3476 Size: 1012 4404 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5416 BstBI TT'CG_AA Cuts at: 4220 4220 Size: 5416 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5416 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2026 Size: 5416 BstYI r'GATC_y Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411 Size: 774 367 385 17 1452 86 12 346 Cuts at: 5411 1972 Size: 1977 Fragments arranged by size: 1977 1452 774 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2289 2335 2512 513 Size: 552 939 27 258 46 177 3417 Fragments arranged by size: 3417 939 552 258 177 46 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4677 Fragments arranged by size: 4677 683 56 BtsI GCAGTG_nn' Cuts at: 2932 3726 3746 2932 Size: 794 20 4602 Fragments arranged by size: 4602 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5416 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5381 DraI TTT'AAA Cuts at: 2720 2720 Size: 5416 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4423 809 Size: 507 3107 1802 Fragments arranged by size: 3107 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2201 2586 Size: 54 1425 50 275 18 198 173 385 Cuts at: 2586 2863 3752 5381 8 Size: 277 889 1629 43 Fragments arranged by size: 1629 1425 889 385 277 275 198 173 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2863 1537 Size: 275 1051 4090 Fragments arranged by size: 4090 1051 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2277 2714 3346 1353 Size: 924 437 632 3423 Fragments arranged by size: 3423 924 632 437 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4003 4377 4523 387 Size: 1064 138 2414 374 146 1280 Fragments arranged by size: 2414 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2231 2301 2504 2525 2585 3479 Size: 393 514 85 70 203 21 60 894 Cuts at: 3479 4076 4863 5131 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 894 787 597 514 393 268 203 85 70 60 21 EcoO109I rG'GnC_Cy Cuts at: 2311 2331 2695 2311 Size: 20 364 5032 Fragments arranged by size: 5032 364 20 EcoRI G'AATT_C Cuts at: 2725 3103 2725 Size: 378 5038 FspI TGC'GCA Cuts at: 3922 3922 Size: 5416 HaeII r_GCGC'y Cuts at: 1678 2414 2477 2497 4563 1678 Size: 736 63 20 2066 2531 Fragments arranged by size: 2531 2066 736 63 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4057 4089 4131 4163 1373 Size: 32 135 32 2485 32 42 32 2626 Fragments arranged by size: 2626 2485 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2760 3006 669 Size: 988 281 822 246 3079 Fragments arranged by size: 3079 988 822 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5416 HpaI GTT'AAC Cuts at: 3006 3006 Size: 5416 KasI G'GCGC_C Cuts at: 2493 2493 Size: 5416 KpnI G_GTAC'C Cuts at: 2741 2741 Size: 5416 MfeI C'AATT_G Cuts at: 3015 3015 Size: 5416 MluI A'CGCG_T Cuts at: 5292 5292 Size: 5416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2427 4530 4714 1305 Size: 1122 2103 184 2007 Fragments arranged by size: 2103 2007 1122 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 10 Size: 54 1425 343 198 3396 Fragments arranged by size: 3396 1425 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 3374 3733 3892 518 Size: 648 2208 359 159 2042 Fragments arranged by size: 2208 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2248 2386 2449 2514 2554 2596 2832 3509 Size: 642 138 63 65 40 42 236 677 Cuts at: 3509 4657 4902 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 245 236 138 65 63 42 40 NarI GG'CG_CC Cuts at: 2494 2494 Size: 5416 NcoI C'CATG_G Cuts at: 513 1065 2004 2031 2289 2335 513 Size: 552 939 27 258 46 3594 Fragments arranged by size: 3594 939 552 258 46 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5416 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5416 NotI GC'GGCC_GC Cuts at: 2863 2863 Size: 5416 NspI r_CATG'y Cuts at: 1132 1229 2774 4319 1132 Size: 97 1545 1545 2229 Fragments arranged by size: 2229 1545 1545 97 PasI CC'CwG_GG Cuts at: 1348 2348 1348 Size: 1000 4416 PciI A'CATG_T Cuts at: 4315 4315 Size: 5416 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5416 PmeI GTTT'AAAC Cuts at: 2720 2720 Size: 5416 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3485 3517 4998 5030 3485 Size: 32 1481 32 3871 Fragments arranged by size: 3871 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2695 2695 Size: 5416 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5416 PsiI TTA'TAA Cuts at: 2986 2986 Size: 5416 PspOMI G'GGCC_C Cuts at: 2311 2330 2311 Size: 19 5397 PspXI vC'TCGA_Gb Cuts at: 2674 2674 Size: 5416 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2501 2533 2501 Size: 32 5384 PstI C_TGCA'G Cuts at: 830 2241 2304 2550 2768 830 Size: 1411 63 246 218 3478 Fragments arranged by size: 3478 1411 246 218 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5416 PvuII CAG'CTG Cuts at: 2248 2386 2449 2596 2248 Size: 138 63 147 5068 Fragments arranged by size: 5068 147 138 63 SacII CC_GC'GG Cuts at: 2515 2515 Size: 5416 SalI G'TCGA_C Cuts at: 1936 2758 1936 Size: 822 4594 SanDI GG'GwC_CC Cuts at: 2695 2695 Size: 5416 SbfI CC_TGCA'GG Cuts at: 2768 2768 Size: 5416 ScaI AGT'ACT Cuts at: 3664 5376 3664 Size: 1712 3704 SfcI C'TryA_G Cuts at: 826 1045 2109 2237 2300 2546 2764 3899 4580 Size: 219 1064 128 63 246 218 1135 681 Cuts at: 4580 4771 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1064 681 246 219 218 191 128 63 SfiI GGCCn_nnn'nGGCC Cuts at: 2100 2618 2627 2100 Size: 518 9 4889 Fragments arranged by size: 4889 518 9 SfoI GGC'GCC Cuts at: 2495 2495 Size: 5416 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5416 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5416 SmaI CCC'GGG Cuts at: 1872 2633 2686 2743 1872 Size: 761 53 57 4545 Fragments arranged by size: 4545 761 57 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 2674 3520 4421 4683 820 Size: 197 926 731 846 901 262 1553 Fragments arranged by size: 1553 926 901 846 731 262 197 SnaBI TAC'GTA Cuts at: 493 3207 493 Size: 2714 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5416 SphI G_CATG'C Cuts at: 1132 2774 1132 Size: 1642 3774 SspI AAT'ATT Cuts at: 5 52 3340 5 Size: 47 3288 2081 Fragments arranged by size: 3288 2081 47 StuI AGG'CCT Cuts at: 1517 2208 2528 1517 Size: 691 320 4405 Fragments arranged by size: 4405 691 320 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2289 2335 2855 513 Size: 552 939 27 258 46 520 3074 Fragments arranged by size: 3074 939 552 520 258 46 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 3443 3460 3613 3798 4243 325 Size: 1255 590 1273 17 153 185 445 1498 Fragments arranged by size: 1498 1273 1255 590 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 3662 5374 Size: 274 80 33 51 1443 243 1442 1712 Cuts at: 5374 96 Size: 138 Fragments arranged by size: 1712 1443 1442 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5376 5408 5376 Size: 32 5384 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5416 XbaI T'CTAG_A Cuts at: 2752 2752 Size: 5416 XhoI C'TCGA_G Cuts at: 1943 2674 1943 Size: 731 4685 XmaI C'CCGG_G Cuts at: 1870 2631 2684 2741 1870 Size: 761 53 57 4545 Fragments arranged by size: 4545 761 57 53 XmnI GAAnn'nnTTC Cuts at: 3545 3545 Size: 5416 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3224 276 Size: 53 83 186 936 1690 2468 Fragments arranged by size: 2468 1690 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI RsrII SacI SapI SexAI SrfI SwaI XcmI