Restriction map for
pFN21AB7803
(Linear) (Six-base) MAPSORT of: KIBB7803.seq Check: 935 from: 1 to: 1509
KIBB7803.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1410 1509
Size: 1410 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 986 1509
Size: 725 261 523
Fragments arranged by size:
725 523 261
AflIII A'CryG_T
Cuts at: 0 325 1509
Size: 325 1184
AhdI GACnn_n'nnGTC
Cuts at: 0 501 1285 1509
Size: 501 784 224
Fragments arranged by size:
784 501 224
AlwNI CAG_nnn'CTG
Cuts at: 0 190 226 586 1509
Size: 190 36 360 923
Fragments arranged by size:
923 360 190 36
ApoI r'AATT_y
Cuts at: 0 575 1509
Size: 575 934
AvaI C'yCGr_G
Cuts at: 0 142 1509
Size: 142 1367
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 500 833 1509
Size: 131 369 333 676
Fragments arranged by size:
676 369 333 131
BbvCI CC'TCA_GC
Cuts at: 0 1256 1509
Size: 1256 253
BglI GCCn_nnn'nGGC
Cuts at: 0 991 1138 1168 1252 1509
Size: 991 147 30 84 257
Fragments arranged by size:
991 257 147 84 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 561 1509
Size: 168 393 948
Fragments arranged by size:
948 393 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1349 1509
Size: 520 829 160
Fragments arranged by size:
829 520 160
Bpu10I CC'TnA_GC
Cuts at: 0 1256 1509
Size: 1256 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1509
Size: 622 887
BsaBI GATnn'nnATC
Cuts at: 0 1105 1509
Size: 1105 404
BsaWI w'CCGG_w
Cuts at: 0 308 728 1509
Size: 308 420 781
Fragments arranged by size:
781 420 308
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1187 1217 1509
Size: 1187 30 292
Fragments arranged by size:
1187 292 30
BseYI C'CCAG_C
Cuts at: 0 62 608 668 1509
Size: 62 546 60 841
Fragments arranged by size:
841 546 62 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 464 1438 1509
Size: 91 373 974 71
Fragments arranged by size:
974 373 91 71
BsiHKAI G_wGCw'C
Cuts at: 0 1323 1509
Size: 1323 186
Bsp1286I G_dGCh'C
Cuts at: 0 1323 1509
Size: 1323 186
BspEI T'CCGG_A
Cuts at: 0 308 728 1509
Size: 308 420 781
Fragments arranged by size:
781 420 308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 211 1410 1509
Size: 66 145 1199 99
Fragments arranged by size:
1199 145 99 66
BssSI C'ACGA_G
Cuts at: 0 371 1509
Size: 371 1138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 108 1509
Size: 108 1401
BstEII G'GTnAC_C
Cuts at: 0 1020 1386 1509
Size: 1020 366 123
Fragments arranged by size:
1020 366 123
BstYI r'GATC_y
Cuts at: 0 1358 1509
Size: 1358 151
BtgI C'CryG_G
Cuts at: 0 1016 1049 1427 1469 1509
Size: 1016 33 378 42 40
Fragments arranged by size:
1016 378 42 40 33
BtsI GCAGTG_nn'
Cuts at: 0 62 1172 1509
Size: 62 1110 337
Fragments arranged by size:
1110 337 62
DraI TTT'AAA
Cuts at: 0 432 1509
Size: 432 1077
DraIII CAC_nnn'GTG
Cuts at: 0 15 1509
Size: 15 1494
DrdI GACnn_nn'nnGTC
Cuts at: 0 1462 1509
Size: 1462 47
EaeI y'GGCC_r
Cuts at: 0 236 1424 1476 1509
Size: 236 1188 52 33
Fragments arranged by size:
1188 236 52 33
EarI CTCTTCn'nnn_
Cuts at: 0 307 743 1509
Size: 307 436 766
Fragments arranged by size:
766 436 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 725 986 1349 1509
Size: 520 205 261 363 160
Fragments arranged by size:
520 363 261 205 160
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1255 1399 1509
Size: 1255 144 110
Fragments arranged by size:
1255 144 110
EcoO109I rG'GnC_Cy
Cuts at: 0 223 1509
Size: 223 1286
EcoRI G'AATT_C
Cuts at: 0 575 1509
Size: 575 934
EcoRV GAT'ATC
Cuts at: 0 711 1451 1509
Size: 711 740 58
Fragments arranged by size:
740 711 58
HaeII r_GCGC'y
Cuts at: 0 1216 1509
Size: 1216 293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 192 224 487 519 1187 1219
Size: 112 32 48 32 263 32 668 32
Cuts at: 1219 1271 1303 1509
Size: 52 32 206
Fragments arranged by size:
668 263 206 112 52 48 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 543 1509
Size: 543 966
HpaI GTT'AAC
Cuts at: 0 543 1509
Size: 543 966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1296 1509
Size: 1296 213
MscI TGG'CCA
Cuts at: 0 1426 1509
Size: 1426 83
MspA1I CmG'CkG
Cuts at: 0 190 217 241 1509
Size: 190 27 24 1268
Fragments arranged by size:
1268 190 27 24
NcoI C'CATG_G
Cuts at: 0 1427 1469 1509
Size: 1427 42 40
Fragments arranged by size:
1427 42 40
NdeI CA'TA_TG
Cuts at: 0 32 1509
Size: 32 1477
NspI r_CATG'y
Cuts at: 0 55 1374 1509
Size: 55 1319 135
Fragments arranged by size:
1319 135 55
PasI CC'CwG_GG
Cuts at: 0 716 1401 1509
Size: 716 685 108
Fragments arranged by size:
716 685 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 698 1143 1383 1485 1509
Size: 698 445 240 102 24
Fragments arranged by size:
698 445 240 102 24
PfoI T'CCnGG_A
Cuts at: 0 488 1509
Size: 488 1021
PpuMI rG'GwC_Cy
Cuts at: 0 223 1509
Size: 223 1286
PshAI GACnn'nnGTC
Cuts at: 0 916 1509
Size: 916 593
PsiI TTA'TAA
Cuts at: 0 809 1509
Size: 809 700
PstI C_TGCA'G
Cuts at: 0 222 1509
Size: 222 1287
PvuII CAG'CTG
Cuts at: 0 190 217 1509
Size: 190 27 1292
Fragments arranged by size:
1292 190 27
SapI GCTCTTCn'nnn_
Cuts at: 0 743 1509
Size: 743 766
ScaI AGT'ACT
Cuts at: 0 643 1509
Size: 643 866
SfcI C'TryA_G
Cuts at: 0 218 647 914 1199 1509
Size: 218 429 267 285 310
Fragments arranged by size:
429 310 285 267 218
SmlI C'TyrA_G
Cuts at: 0 637 1509
Size: 637 872
StyI C'CwwG_G
Cuts at: 0 1427 1469 1509
Size: 1427 42 40
Fragments arranged by size:
1427 42 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1150 1509
Size: 1150 359
TatI w'GTAC_w
Cuts at: 0 473 641 1509
Size: 473 168 868
Fragments arranged by size:
868 473 168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1140 1342 1509
Size: 1140 202 167
Fragments arranged by size:
1140 202 167
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApoI AvaI BbsI
BbvCI BglI BplI BpmI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BssSI
BstAPI BstEII BstYI BtgI BtsI DraI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HpaI MmeI MscI MspA1I NcoI NdeI
NspI PasI PflMI PfoI PpuMI PshAI PsiI PstI
PvuII SapI ScaI SfcI SmlI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BsaI
BsaAI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI EagI EciI EcoICRI FalI
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI