(Circular) (Six-base) MAPSORT of: pFN21AB7759.seq Check: 9648 from: 1 to: 6046 pFN21AB7759 6046 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2208 2552 2561 2894 4109 5493 5761 1632 Size: 576 344 9 333 1215 1384 268 1917 Fragments arranged by size: 1917 1384 1215 576 344 333 268 9 AfeI AGC'GCT Cuts at: 2762 2762 Size: 6046 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 3158 1166 Size: 1992 4054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2781 3297 1184 Size: 1597 516 3933 Fragments arranged by size: 3933 1597 516 AlwNI CAG_nnn'CTG Cuts at: 2115 3789 5361 2115 Size: 1674 1572 2800 Fragments arranged by size: 2800 1674 1572 ApaI G_GGCC'C Cuts at: 2026 2026 Size: 6046 ApaLI G'TGCA_C Cuts at: 3338 4103 5259 3338 Size: 765 1156 4125 Fragments arranged by size: 4125 1156 765 ApoI r'AATT_y Cuts at: 1493 3005 3355 3581 3733 5842 1493 Size: 1512 350 226 152 2109 1697 Fragments arranged by size: 2109 1697 1512 350 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2320 3371 1870 Size: 73 377 1051 4545 Fragments arranged by size: 4545 1051 377 73 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2725 3367 4822 618 Size: 325 217 627 938 642 1455 1842 Fragments arranged by size: 1842 1455 938 642 627 325 217 BanII G_rGCy'C Cuts at: 1391 2026 2193 2220 3365 1391 Size: 635 167 27 1145 4072 Fragments arranged by size: 4072 1145 635 167 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2882 2982 928 Size: 971 983 100 3992 Fragments arranged by size: 3992 983 971 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2412 4657 136 Size: 107 122 71 631 605 740 2245 1525 Fragments arranged by size: 2245 1525 740 631 605 122 107 71 BglII A'GATC_T Cuts at: 3327 6041 3327 Size: 2714 3332 BlpI GC'TnA_GC Cuts at: 2621 2846 3463 2621 Size: 225 617 5204 Fragments arranged by size: 5204 617 225 Bme1580I G_kGCm'C Cuts at: 1790 2026 3342 4107 5263 1790 Size: 236 1316 765 1156 2573 Fragments arranged by size: 2573 1316 1156 765 236 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2645 3777 4735 Size: 416 53 83 399 24 1228 1132 958 Cuts at: 4735 442 Size: 1753 Fragments arranged by size: 1753 1228 1132 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2924 3016 3116 4706 1239 Size: 959 726 92 100 1590 2579 Fragments arranged by size: 2579 1590 959 726 100 92 Bpu10I CC'TnA_GC Cuts at: 3254 3254 Size: 6046 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2936 4171 5036 5334 2936 Size: 1235 865 298 3648 Fragments arranged by size: 3648 1235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4709 882 Size: 617 3210 2219 Fragments arranged by size: 3210 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3853 4235 4870 275 Size: 53 83 186 936 2320 382 635 1451 Fragments arranged by size: 2320 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3246 4479 4877 5151 5298 5709 3246 Size: 1233 398 274 147 411 3583 Fragments arranged by size: 3583 1233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2669 2699 2923 2953 1178 Size: 30 165 30 1266 30 224 30 4271 Fragments arranged by size: 4271 1266 224 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2567 1186 Size: 1327 54 4665 Fragments arranged by size: 4665 1327 54 BseYI C'CCAG_C Cuts at: 2017 2153 2281 2616 2664 5249 5745 2017 Size: 136 128 335 48 2585 496 2318 Fragments arranged by size: 2585 2318 496 335 136 128 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 3140 1163 Size: 573 1083 321 4069 Fragments arranged by size: 4069 1083 573 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2889 3496 4257 5285 1540 Size: 275 186 888 607 761 1028 2301 Fragments arranged by size: 2301 1028 888 761 607 275 186 BsiHKAI G_wGCw'C Cuts at: 3342 4107 4192 5263 3342 Size: 765 85 1071 4125 Fragments arranged by size: 4125 1071 765 85 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 BsmBI CGTCTCn'nnnn_ Cuts at: 2797 3187 2797 Size: 390 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2193 2220 3342 3365 4107 4192 Size: 399 236 167 27 1122 23 742 85 Cuts at: 4192 5263 1391 Size: 1071 2174 Fragments arranged by size: 2174 1122 1071 742 399 236 167 85 27 23 BspEI T'CCGG_A Cuts at: 3246 5709 3246 Size: 2463 3583 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3401 844 Size: 2557 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3081 4541 4715 65 Size: 1183 1833 1460 174 1396 Fragments arranged by size: 1833 1460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2679 2697 3108 4690 4877 1700 Size: 110 869 18 411 1582 187 2869 Fragments arranged by size: 2869 1582 869 411 187 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 2084 2115 794 Size: 1290 31 4725 Fragments arranged by size: 4725 1290 31 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstXI CCAn_nnnn'nTGG Cuts at: 2153 2281 2153 Size: 128 5918 BstYI r'GATC_y Cuts at: 1972 2238 2523 2731 3327 3376 3743 4128 4145 Size: 266 285 208 596 49 367 385 17 Cuts at: 4145 5597 5683 5695 6041 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 596 385 367 346 285 266 208 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2427 2721 3218 513 Size: 552 1362 294 497 3341 Fragments arranged by size: 3341 1362 552 497 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 2419 3284 3562 4356 4376 2419 Size: 865 278 794 20 4089 Fragments arranged by size: 4089 865 794 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2416 2784 3493 Size: 54 1425 50 275 18 586 368 709 Cuts at: 3493 4382 6011 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 709 586 368 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3976 1353 Size: 2623 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2360 2516 4633 5007 5153 387 Size: 1064 138 771 156 2117 374 146 1280 Fragments arranged by size: 2117 1280 1064 771 374 156 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2208 2552 2561 2894 2924 3016 Size: 393 566 10 344 9 333 30 92 Cuts at: 3016 3116 4109 4706 5493 5761 1239 Size: 100 993 597 787 268 1524 Fragments arranged by size: 1524 993 787 597 566 393 344 333 268 100 92 30 10 9 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2126 Size: 6046 EcoO109I rG'GnC_Cy Cuts at: 2022 2023 2022 Size: 1 6045 EcoRI G'AATT_C Cuts at: 3005 3355 3733 3005 Size: 350 378 5318 Fragments arranged by size: 5318 378 350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 6046 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 2686 2764 5193 1678 Size: 1008 78 2429 2531 Fragments arranged by size: 2531 2429 1008 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 4687 4719 4761 Size: 32 135 32 731 32 2352 32 42 Cuts at: 4761 4793 1373 Size: 32 2626 Fragments arranged by size: 2626 2352 731 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2812 3390 3636 669 Size: 988 281 874 578 246 3079 Fragments arranged by size: 3079 988 874 578 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5160 5344 1305 Size: 3855 184 2007 Fragments arranged by size: 3855 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 10 Size: 54 1425 343 954 3270 Fragments arranged by size: 3270 1425 954 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2334 2438 3156 3158 3198 4004 4363 Size: 648 1168 104 718 2 40 806 359 Cuts at: 4363 4522 518 Size: 159 2042 Fragments arranged by size: 2042 1168 806 718 648 359 159 104 40 2 MspA1I CmG'CkG Cuts at: 1606 2115 3316 3462 4139 5287 5532 1606 Size: 509 1201 146 677 1148 245 2120 Fragments arranged by size: 2120 1201 1148 677 509 245 146 NcoI C'CATG_G Cuts at: 513 1065 2427 2721 513 Size: 552 1362 294 3838 Fragments arranged by size: 3838 1362 552 294 NdeI CA'TA_TG Cuts at: 387 3157 387 Size: 2770 3276 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 2443 2494 3176 3404 4949 1132 Size: 97 1214 51 682 228 1545 2229 Fragments arranged by size: 2229 1545 1214 682 228 97 51 PasI CC'CwG_GG Cuts at: 1348 2101 2586 2927 1348 Size: 753 485 341 4467 Fragments arranged by size: 4467 753 485 341 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 2967 3616 2967 Size: 649 5397 PspOMI G'GGCC_C Cuts at: 2022 2022 Size: 6046 PstI C_TGCA'G Cuts at: 830 2167 2188 2844 3106 3150 3297 3398 830 Size: 1337 21 656 262 44 147 101 3478 Fragments arranged by size: 3478 1337 656 262 147 101 44 21 PvuI CG_AT'CG Cuts at: 2001 2889 2001 Size: 888 5158 PvuII CAG'CTG Cuts at: 2115 2115 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2163 2184 2840 3102 3146 3293 3394 Size: 219 1118 21 656 262 44 147 101 Cuts at: 3394 4529 5210 5401 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1118 681 656 262 219 191 147 101 44 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 3373 1872 Size: 1501 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2951 4150 5051 5313 820 Size: 197 926 1008 1199 901 262 1553 Fragments arranged by size: 1553 1199 1008 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 2494 3176 3404 1132 Size: 1362 682 228 3774 Fragments arranged by size: 3774 1362 682 228 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 2318 1517 Size: 801 5245 StyI C'CwwG_G Cuts at: 513 1065 2427 2714 2721 3485 513 Size: 552 1362 287 7 764 3074 Fragments arranged by size: 3074 1362 764 552 287 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3219 4073 4090 4243 4428 4873 325 Size: 1255 1639 854 17 153 185 445 1498 Fragments arranged by size: 1639 1498 1255 854 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4292 6004 96 Size: 274 80 33 51 1443 2315 1712 138 Fragments arranged by size: 2315 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6006 6038 6006 Size: 32 6014 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 3371 1870 Size: 1501 4545 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI