(Circular) (Six-base) MAPSORT of: pFN21AB6482.seq Check: 4084 from: 1 to: 5773 pFN21AB6482 5773 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 3836 5220 5488 1632 Size: 797 1407 1384 268 1917 Fragments arranged by size: 1917 1407 1384 797 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 2427 1166 Size: 1261 4512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2864 1184 Size: 1680 4093 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaI G_GGCC'C Cuts at: 2129 2501 2129 Size: 372 5401 ApaLI G'TGCA_C Cuts at: 2886 3830 4986 2886 Size: 944 1156 3673 Fragments arranged by size: 3673 1156 944 ApoI r'AATT_y Cuts at: 1493 3082 3308 3460 5569 1493 Size: 1589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1589 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 2128 2906 3098 1870 Size: 73 185 778 192 4545 Fragments arranged by size: 4545 778 192 185 73 AvrII C'CTAG_G Cuts at: 2281 2281 Size: 5773 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2460 3094 4549 618 Size: 325 217 627 567 106 634 1455 1842 Fragments arranged by size: 1842 1455 634 627 567 325 217 106 BanII G_rGCy'C Cuts at: 1391 2072 2129 2146 2501 2718 2735 2899 2907 Size: 681 57 17 355 217 17 164 8 Cuts at: 2907 3092 1391 Size: 185 4072 Fragments arranged by size: 4072 681 355 217 185 164 57 17 17 8 BbeI G_GCGC'C Cuts at: 2358 2464 2358 Size: 106 5667 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 928 Size: 971 247 4555 Fragments arranged by size: 4555 971 247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2818 2852 3964 3998 1903 Size: 34 881 34 1112 34 3678 Fragments arranged by size: 3678 1112 881 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4384 136 Size: 107 122 71 631 605 2712 1525 Fragments arranged by size: 2712 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2129 2422 2434 2440 2501 2890 3834 4990 Size: 339 293 12 6 61 389 944 1156 Cuts at: 4990 1790 Size: 2573 Fragments arranged by size: 2573 1156 944 389 339 293 61 12 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 2712 2795 4433 1239 Size: 1385 88 83 1638 2579 Fragments arranged by size: 2579 1638 1385 88 83 Bpu10I CC'TnA_GC Cuts at: 2493 2901 2493 Size: 408 5365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 882 Size: 617 2937 2219 Fragments arranged by size: 2937 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2641 2721 3564 493 Size: 711 1437 80 843 2702 Fragments arranged by size: 2702 1437 843 711 80 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2355 2461 3580 3962 Size: 53 83 186 936 822 106 1119 382 Cuts at: 3962 4597 275 Size: 635 1451 Fragments arranged by size: 1451 1119 936 822 635 382 186 106 83 53 BsaWI w'CCGG_w Cuts at: 2080 2710 4206 4604 4878 5025 5436 2080 Size: 630 1496 398 274 147 411 2417 Fragments arranged by size: 2417 1496 630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2357 2705 2735 1178 Size: 30 165 30 924 30 348 30 4216 Fragments arranged by size: 4216 924 348 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2588 2597 2870 1186 Size: 1402 9 273 4089 Fragments arranged by size: 4089 1402 273 9 BseYI C'CCAG_C Cuts at: 2009 3034 4976 5472 2009 Size: 1025 1942 496 2310 Fragments arranged by size: 2310 1942 1025 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2282 2381 1163 Size: 573 438 108 99 4555 Fragments arranged by size: 4555 573 438 108 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2250 2728 3223 3984 5012 1540 Size: 275 186 249 478 495 761 1028 2301 Fragments arranged by size: 2301 1028 761 495 478 275 249 186 BsiHKAI G_wGCw'C Cuts at: 2890 3834 3919 4990 2890 Size: 944 85 1071 3673 Fragments arranged by size: 3673 1071 944 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 BsmBI CGTCTCn'nnnn_ Cuts at: 2958 2958 Size: 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2129 2146 2422 2434 2440 2501 Size: 399 282 57 17 276 12 6 61 Cuts at: 2501 2718 2735 2890 2899 2907 3092 3834 3919 Size: 217 17 155 9 8 185 742 85 Cuts at: 3919 4990 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 276 217 185 155 85 61 57 17 17 12 9 8 6 BspEI T'CCGG_A Cuts at: 2080 5436 2080 Size: 3356 2417 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 3128 844 Size: 1433 851 3489 Fragments arranged by size: 3489 1433 851 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2249 4417 4604 1700 Size: 110 439 2168 187 2869 Fragments arranged by size: 2869 2168 439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstYI r'GATC_y Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768 Size: 1131 367 385 17 1452 86 12 346 Cuts at: 5768 1972 Size: 1977 Fragments arranged by size: 1977 1452 1131 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2786 2786 Size: 5773 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 3069 513 Size: 552 939 324 741 3217 Fragments arranged by size: 3217 939 741 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2988 487 Size: 683 56 1762 3272 Fragments arranged by size: 3272 1762 683 56 BtsI GCAGTG_nn' Cuts at: 2186 2754 2848 3289 4083 4103 2186 Size: 568 94 441 794 20 3856 Fragments arranged by size: 3856 794 568 441 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 2643 2725 Size: 54 1425 50 275 18 417 396 82 Cuts at: 2725 2824 3220 4109 5738 8 Size: 99 396 889 1629 43 Fragments arranged by size: 1629 1425 889 417 396 396 275 99 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2247 2725 3220 1537 Size: 275 435 478 495 4090 Fragments arranged by size: 4090 495 478 435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2565 3054 3703 1353 Size: 1212 489 649 3423 Fragments arranged by size: 3423 1212 649 489 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2387 4360 4734 4880 387 Size: 1064 138 798 1973 374 146 1280 Fragments arranged by size: 1973 1280 1064 798 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2624 2712 2795 3836 4433 5220 Size: 393 797 195 88 83 1041 597 787 Cuts at: 5220 5488 1239 Size: 268 1524 Fragments arranged by size: 1524 1041 797 787 597 393 268 195 88 83 EcoNI CCTnn'n_nnAGG Cuts at: 2492 2492 Size: 5773 EcoO109I rG'GnC_Cy Cuts at: 2102 2497 2498 2102 Size: 395 1 5377 Fragments arranged by size: 5377 395 1 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 2358 2396 2464 4920 1678 Size: 680 38 68 2456 2531 Fragments arranged by size: 2531 2456 680 68 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2909 2941 4414 4446 4488 Size: 32 135 32 1337 32 1473 32 42 Cuts at: 4488 4520 1373 Size: 32 2626 Fragments arranged by size: 2626 1473 1337 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KasI G'GCGC_C Cuts at: 2354 2460 2354 Size: 106 5667 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 4887 5071 1305 Size: 1272 2310 184 2007 Fragments arranged by size: 2310 2007 1272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3951 Fragments arranged by size: 3951 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 2427 2658 3731 4090 4249 518 Size: 648 1045 216 231 1073 359 159 2042 Fragments arranged by size: 2042 1073 1045 648 359 231 216 159 MspA1I CmG'CkG Cuts at: 1606 2061 2090 2602 2961 3189 3866 5014 5259 Size: 455 29 512 359 228 677 1148 245 Cuts at: 5259 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 512 455 359 245 228 29 NaeI GCC'GGC Cuts at: 2251 2251 Size: 5773 NarI GG'CG_CC Cuts at: 2355 2461 2355 Size: 106 5667 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4282 Fragments arranged by size: 4282 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NgoMIV G'CCGG_C Cuts at: 2249 2249 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 2818 3131 4676 1132 Size: 97 1589 313 1545 2229 Fragments arranged by size: 2229 1589 1545 313 97 PasI CC'CwG_GG Cuts at: 1348 2074 2466 1348 Size: 726 392 4655 Fragments arranged by size: 4655 726 392 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5773 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PmlI CAC'GTG Cuts at: 2641 2721 2641 Size: 80 5693 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2102 2102 Size: 5773 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5773 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PspOMI G'GGCC_C Cuts at: 2125 2497 2125 Size: 372 5401 PstI C_TGCA'G Cuts at: 830 2274 2601 2939 3125 830 Size: 1444 327 338 186 3478 Fragments arranged by size: 3478 1444 338 327 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 2602 2602 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2270 2597 2935 3121 4256 4937 5128 Size: 219 1225 327 338 186 1135 681 191 Cuts at: 5128 826 Size: 1471 Fragments arranged by size: 1471 1225 1135 681 338 327 219 191 186 SfoI GGC'GCC Cuts at: 2356 2462 2356 Size: 106 5667 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 2130 3100 1872 Size: 258 970 4545 Fragments arranged by size: 4545 970 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 3877 4778 5040 820 Size: 197 926 1934 901 262 1553 Fragments arranged by size: 1934 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 2004 2281 3212 513 Size: 552 939 277 931 3074 Fragments arranged by size: 3074 939 931 552 277 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 3817 3970 4155 4600 325 Size: 1255 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4019 5731 Size: 274 80 33 51 1443 282 1760 1712 Cuts at: 5731 96 Size: 138 Fragments arranged by size: 1760 1712 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2705 2737 5733 5765 2705 Size: 32 2996 32 2713 Fragments arranged by size: 2996 2713 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 2128 3098 1870 Size: 258 970 4545 Fragments arranged by size: 4545 970 258 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI