Restriction map for
pFN21AB5411
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BsaBI BsaI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1384 CGCG CG^CG
AcsI 295 AAATTT [GA]^AATT[CT]
AcyI 88 GACGCC G[GA]^CG[CT]C
AhdI 1507 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 403 CCTGAGG CC^T[ACGT]AGG
ApoI 295 AAATTT [GA]^AATT[CT]
Asp700I 499 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1507 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 844 GTCTTC GAAGAC(2/6)
BbvCI 1321 GCTGAGG CCTCAGC(-5/-2)
BcnI 5 CCGGG CC^[GC]GG
BfaI 989 CTAG C^TAG
BlpI 1213 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1213 GCTGAGC GC^T[ACGT]AGC
BpuAI 844 GTCTTC GAAGAC(2/6)
BsaAI 1437 CACGTA [CT]AC^GT[GA]
BsaHI 88 GACGCC G[GA]^CG[CT]C
BsgI 1681 CTGCAC GTGCAG(16/14)
BsmFI 1758 GTCCC GGGAC(10/14)
BsmI 309 GAATGC GAATGC(1/-1)
BspHI 249 TCATGA T^CATGA
BspMI 1370 ACCTGC ACCTGC(4/8)
BsrBI 1003 GAGCGG CCGCTC(-3/-3)
BssSI 1053 CACGAG CACGAG(-5/-1)
BstUI 1384 CGCG CG^CG
Bsu36I 403 CCTGAGG CC^T[ACGT]AGG
CelII 1213 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1507 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 403 CCTGAGG CC^T[ACGT]AGG
HgaI 88 GACGC GACGC(5/10)
Hin1I 88 GACGCC G[GA]^CG[CT]C
MaeI 989 CTAG C^TAG
MfeI 105 CAATTG C^AATTG
MslI 724 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 105 CAATTG C^AATTG
MvnI 1384 CGCG CG^CG
NciI 5 CCGGG CC^[GC]GG
PaeR7I 1019 CTCGAG C^TCGAG
RcaI 249 TCATGA T^CATGA
SacI 1780 GAGCTC GAGCT^C
SbfI 1371 CCTGCAGG CCTGCA^GG
SexAI 175 ACCTGGT A^CC[AT]GGT
SfiI 1194 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1371 CCTGCAGG CCTGCA^GG
StuI 1640 AGGCCT AGG^CCT
XhoI 1019 CTCGAG C^TCGAG
XmnI 499 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 540 TCCGGA T^CCGGA
671 TCCGGA
AlwI 74 GGATC GGATC(4/5)
246 GGATC
AvaI 1019 CTCGAG C^[CT]CG[GA]G
1760 CCCGAG
BalI 290 TGGCCA TGG^CCA
1106 TGGCCA
BanII 163 GGGCTC G[GA]GC[CT]^C
1780 GAGCTC
BglII 389 AGATCT A^GATCT
758 AGATCT
BsaWI 540 TCCGGA [AT]^CCGG[AT]
671 TCCGGA
BseAI 540 TCCGGA T^CCGGA
671 TCCGGA
BsmAI 1135 GAGAC GTCTC(1/5)
1663 GAGAC
BsoBI 1019 CTCGAG C^[CT]CG[GA]G
1760 CCCGAG
BspEI 540 TCCGGA T^CCGGA
671 TCCGGA
BstYI 389 AGATCT [GA]^GATC[CT]
758 AGATCT
EcoT14I 83 CCATGG C^C[AT][AT]GG
1553 CCATGG
HindIII 865 AAGCTT A^AGCTT
1488 AAGCTT
MaeII 61 ACGT A^CGT
1438 ACGT
MflI 389 AGATCT [GA]^GATC[CT]
758 AGATCT
MluNI 290 TGGCCA TGG^CCA
1106 TGGCCA
MroI 540 TCCGGA T^CCGGA
671 TCCGGA
MscI 290 TGGCCA TGG^CCA
1106 TGGCCA
MspA1I 1139 CAGCTG C[AC]G^C[GT]G
1357 CAGCTG
NcoI 83 CCATGG C^CATGG
1553 CCATGG
PvuII 1139 CAGCTG CAG^CTG
1357 CAGCTG
SapI 1397 GAAGAGC GCTCTTC(1/4)
1777 GAAGAGC
StyI 83 CCATGG C^C[AT][AT]GG
1553 CCATGG
XcmI 438 CCAGGCCAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1754 CCAAGTCCCGAGTGG
XhoII 389 AGATCT [GA]^GATC[CT]
758 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BbvI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I