(Circular) (Six-base) MAPSORT of: pFN21AB5411.seq Check: 8984 from: 1 to: 6490 pFN21AB5411 6490 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3833 1937 Size: 1896 4594 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2020 2340 2648 3338 3386 4553 5937 6205 Size: 388 320 308 690 48 1167 1384 268 Cuts at: 6205 1632 Size: 1917 Fragments arranged by size: 1917 1384 1167 690 388 320 308 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 5389 6366 1203 Size: 4186 977 1327 Fragments arranged by size: 4186 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 3517 5219 3517 Size: 1702 4788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2714 3623 1184 Size: 1530 909 4051 Fragments arranged by size: 4051 1530 909 AlwNI CAG_nnn'CTG Cuts at: 2644 3218 3376 4233 5805 2644 Size: 574 158 857 1572 3329 Fragments arranged by size: 3329 1572 857 574 158 ApaLI G'TGCA_C Cuts at: 4547 5703 4547 Size: 1156 5334 ApoI r'AATT_y Cuts at: 1493 2300 3799 4025 4177 6286 1493 Size: 807 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 807 226 152 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 3024 3765 3815 1870 Size: 73 1081 741 50 4545 Fragments arranged by size: 4545 1081 741 73 50 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3811 5266 618 Size: 325 217 627 2024 1455 1842 Fragments arranged by size: 2024 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 3789 3809 1391 Size: 781 1617 20 4072 Fragments arranged by size: 4072 1617 781 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2842 928 Size: 971 943 4576 Fragments arranged by size: 4576 971 943 BbvCI CC'TCA_GC Cuts at: 3327 3327 Size: 6490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2444 3206 3215 Size: 107 122 71 631 605 772 762 9 Cuts at: 3215 3353 5101 136 Size: 138 1748 1525 Fragments arranged by size: 1748 1525 772 762 631 605 138 122 107 71 9 BglII A'GATC_T Cuts at: 2394 2763 6485 2394 Size: 369 3722 2399 Fragments arranged by size: 3722 2399 369 BlpI GC'TnA_GC Cuts at: 3219 3907 3219 Size: 688 5802 Bme1580I G_kGCm'C Cuts at: 1790 2693 4551 5707 1790 Size: 903 1858 1156 2573 Fragments arranged by size: 2573 1858 1156 903 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2463 4221 5179 Size: 416 53 83 399 24 1046 1758 958 Cuts at: 5179 442 Size: 1753 Fragments arranged by size: 1758 1753 1046 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2666 2879 3443 5150 1239 Size: 1427 213 564 1707 2579 Fragments arranged by size: 2579 1707 1427 564 213 Bpu10I CC'TnA_GC Cuts at: 2637 3327 2637 Size: 690 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 3614 4615 5480 5778 2030 Size: 1584 1001 865 298 2742 Fragments arranged by size: 2742 1584 1001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 4281 493 Size: 711 2240 837 2702 Fragments arranged by size: 2702 2240 837 711 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 4297 4679 5314 Size: 53 83 186 936 561 2203 382 635 Cuts at: 5314 275 Size: 1451 Fragments arranged by size: 2203 1451 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2545 2676 4923 5321 5595 5742 6153 2545 Size: 131 2247 398 274 147 411 2882 Fragments arranged by size: 2882 2247 411 398 274 147 131 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6265 Fragments arranged by size: 6265 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2573 2854 2944 2999 3059 3091 3152 Size: 1153 234 281 90 55 60 32 61 Cuts at: 3152 3194 3197 3278 3281 3344 3355 1186 Size: 42 3 81 3 63 11 4321 Fragments arranged by size: 4321 1153 281 234 90 81 63 61 60 55 42 32 11 3 3 BseYI C'CCAG_C Cuts at: 5693 6189 5693 Size: 496 5994 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3671 1163 Size: 573 1935 3982 Fragments arranged by size: 3982 1935 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 2589 3789 4551 4636 5707 2424 Size: 165 1200 762 85 1071 3207 Fragments arranged by size: 3207 1200 1071 762 165 85 BsmI GAATG_Cn' Cuts at: 2320 4001 4094 2320 Size: 1681 93 4716 Fragments arranged by size: 4716 1681 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2424 2589 2693 3789 3809 4551 Size: 399 382 252 165 104 1096 20 742 Cuts at: 4551 4636 5707 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1096 1071 742 399 382 252 165 104 85 20 BspEI T'CCGG_A Cuts at: 2545 2676 6153 2545 Size: 131 3477 2882 Fragments arranged by size: 3477 2882 131 BspHI T'CATG_A Cuts at: 2254 4379 6109 2254 Size: 2125 1730 2635 Fragments arranged by size: 2635 2125 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3384 3845 844 Size: 2540 461 3489 Fragments arranged by size: 3489 2540 461 BsrBI CCG'CTC Cuts at: 1394 1823 3010 4377 1394 Size: 429 1187 1367 3507 Fragments arranged by size: 3507 1367 1187 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4985 5159 65 Size: 1183 3737 174 1396 Fragments arranged by size: 3737 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 3058 4550 5562 3058 Size: 1492 1012 3986 Fragments arranged by size: 3986 1492 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3229 3292 3376 794 Size: 2435 63 84 3908 Fragments arranged by size: 3908 2435 84 63 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 2394 2763 3820 4187 4572 4589 6041 6127 Size: 422 369 1057 367 385 17 1452 86 Cuts at: 6127 6139 6485 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1057 422 385 369 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2409 2409 Size: 6490 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2774 3558 513 Size: 552 939 84 686 784 3445 Fragments arranged by size: 3445 939 784 686 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2002 487 Size: 683 56 776 4975 Fragments arranged by size: 4975 776 683 56 BtsI GCAGTG_nn' Cuts at: 4006 4800 4820 4006 Size: 794 20 5676 Fragments arranged by size: 5676 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2295 3111 Size: 54 1425 50 275 18 177 288 816 Cuts at: 3111 3937 4826 6455 8 Size: 826 889 1629 43 Fragments arranged by size: 1629 1425 889 826 816 288 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 3397 3777 4420 1353 Size: 963 1081 380 643 3423 Fragments arranged by size: 3423 1081 963 643 380 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3410 5077 5451 5597 387 Size: 1064 138 1821 1667 374 146 1280 Fragments arranged by size: 1821 1667 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3787 3787 Size: 6490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2340 2648 2666 2879 3338 3386 Size: 393 388 320 308 18 213 459 48 Cuts at: 3386 3443 4553 5150 5937 6205 1239 Size: 57 1110 597 787 268 1524 Fragments arranged by size: 1524 1110 787 597 459 393 388 320 308 268 213 57 48 18 EcoO109I rG'GnC_Cy Cuts at: 2137 2641 3373 2137 Size: 504 732 5254 Fragments arranged by size: 5254 732 504 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2158 2785 3763 2158 Size: 627 978 4885 Fragments arranged by size: 4885 978 627 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 3019 3277 3759 5637 1678 Size: 1341 258 482 1878 2531 Fragments arranged by size: 2531 1878 1341 482 258 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3503 3535 3589 3621 5131 Size: 32 135 32 1931 32 54 32 1510 Cuts at: 5131 5163 5205 5237 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1931 1510 135 54 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 2870 3493 748 Size: 2122 623 3745 Fragments arranged by size: 3745 2122 623 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 2110 4089 2110 Size: 1979 4511 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 3726 5604 5788 1305 Size: 1746 675 1878 184 2007 Fragments arranged by size: 2007 1878 1746 675 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 3113 10 Size: 54 1425 343 465 816 3387 Fragments arranged by size: 3387 1425 816 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2733 4448 4807 4966 518 Size: 648 1567 1715 359 159 2042 Fragments arranged by size: 2042 1715 1567 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3146 3364 3906 4583 5731 5976 1606 Size: 1540 218 542 677 1148 245 2120 Fragments arranged by size: 2120 1540 1148 677 542 245 218 NcoI C'CATG_G Cuts at: 513 1065 2004 2088 3558 513 Size: 552 939 84 1470 3445 Fragments arranged by size: 3445 1470 939 552 84 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 3848 5393 1132 Size: 97 2619 1545 2229 Fragments arranged by size: 2619 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2719 1348 Size: 1371 5119 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6490 PfoI T'CCnGG_A Cuts at: 3420 3420 Size: 6490 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2090 2122 4559 4591 6072 6104 2090 Size: 32 2437 32 1481 32 2476 Fragments arranged by size: 2476 2437 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2641 3373 2137 Size: 504 732 5254 Fragments arranged by size: 5254 732 504 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PstI C_TGCA'G Cuts at: 830 2199 3090 3381 3612 3842 830 Size: 1369 891 291 231 230 3478 Fragments arranged by size: 3478 1369 891 291 231 230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 3146 3364 3146 Size: 218 6272 SacI G_AGCT'C Cuts at: 3789 3789 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SapI GCTCTTCn'nnn_ Cuts at: 3397 3777 3397 Size: 380 6110 SbfI CC_TGCA'GG Cuts at: 3381 3842 3381 Size: 461 6029 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SexAI A'CCwGG_T Cuts at: 2180 2180 Size: 6490 SfcI C'TryA_G Cuts at: 826 1045 2195 3086 3377 3608 3838 4973 5654 Size: 219 1150 891 291 231 230 1135 681 Cuts at: 5654 5845 826 Size: 191 1471 Fragments arranged by size: 1471 1150 1135 891 681 291 231 230 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3206 3206 Size: 6490 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3024 3629 4594 5495 5757 Size: 197 926 102 979 605 965 901 262 Cuts at: 5757 820 Size: 1553 Fragments arranged by size: 1553 979 965 926 901 605 262 197 102 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 3647 1517 Size: 2130 4360 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 3558 3929 513 Size: 552 939 84 1470 371 3074 Fragments arranged by size: 3074 1470 939 552 371 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4534 4687 4872 5317 325 Size: 1255 2937 17 153 185 445 1498 Fragments arranged by size: 2937 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 4736 6448 Size: 274 80 33 51 1443 222 2537 1712 Cuts at: 6448 96 Size: 138 Fragments arranged by size: 2537 1712 1443 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6450 6482 6450 Size: 32 6458 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2450 3766 2450 Size: 1316 5174 XhoI C'TCGA_G Cuts at: 1943 3024 1943 Size: 1081 5409 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 2508 4619 2508 Size: 2111 4379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI