Restriction map for pFN21AB6861
 (Circular) (Six-base) MAPSORT of: pFN21AB6861.seq  Check: 8512  from: 1  to: 5242



pFN21AB6861  5242 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:43  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3052     278 
   Size:         53      83     186     936    1516    2468

  Fragments arranged by size: 

                2468    1516     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2585    1937 
   Size:        648    4594
 
Acc65I G'GTAC_C

Cuts at:   2220    2563    2220 
   Size:        343    4899
 
AclI AA'CG_TT

Cuts at:   1563    3369    3742    1563 
   Size:       1806     373    3063

  Fragments arranged by size: 

                3063    1806     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2216    2478    3305    4689    4957    1632 
   Size:        584     262     827    1384     268    1917

  Fragments arranged by size: 

                1917    1384     827     584     268     262
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5045
 
AflIII A'CryG_T

Cuts at:   1203    4141    5118    1203 
   Size:       2938     977    1327

  Fragments arranged by size: 

                2938    1327     977
 
AgeI A'CCGG_T

Cuts at:   4073    4073 
   Size:       5242
 
AhdI GACnn_n'nnGTC

Cuts at:   3971    3971 
   Size:       5242
 
AleI CACnn'nnGTG

Cuts at:   1166    2503    1166 
   Size:       1337    3905
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5242
 
AlwNI CAG_nnn'CTG

Cuts at:   2194    2985    4557    2194 
   Size:        791    1572    2879

  Fragments arranged by size: 

                2879    1572     791
 
ApaLI G'TGCA_C

Cuts at:   3299    4455    3299 
   Size:       1156    4086
 
ApoI r'AATT_y

Cuts at:   1493    2213    2551    2777    2929    5038    1493 
   Size:        720     338     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     720     338     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3796     160 
   Size:        872    2764    1606

  Fragments arranged by size: 

                2764    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5242
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2084    2526    2567    1870 
   Size:         73     141     442      41    4545

  Fragments arranged by size: 

                4545     442     141      73      41
 
BamHI G'GATC_C

Cuts at:   2572    2939    2572 
   Size:        367    4875
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2220    2267    2563    4018     618 
   Size:        325     217     627     433      47     296    1455    1842

  Fragments arranged by size: 

                1842    1455     627     433     325     296     217      47
            
 
BanII G_rGCy'C

Cuts at:   1391    2143    2561    1391 
   Size:        752     418    4072

  Fragments arranged by size: 

                4072     752     418
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4271
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3433    3467    1903 
   Size:         34    1496      34    3678

  Fragments arranged by size: 

                3678    1496      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2358    3134    4344    2358 
   Size:        776    1210    3256

  Fragments arranged by size: 

                3256    1210     776
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5242
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3853     136 
   Size:        107     122      71     631     605    2181    1525

  Fragments arranged by size: 

                2181    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5237    5237 
   Size:       5242
 
BlpI GC'TnA_GC

Cuts at:   2659    2659 
   Size:       5242
 
Bme1580I G_kGCm'C

Cuts at:   1790    2270    3303    4459    1790 
   Size:        480    1033    1156    2573

  Fragments arranged by size: 

                2573    1156    1033     480
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5242
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2973    3931     442 
   Size:        416      53      83     399      24    1556     958    1753

  Fragments arranged by size: 

                1753    1556     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5242
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3902    1239 
   Size:       2663    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3367    4232    4530    3367 
   Size:        865     298    4079

  Fragments arranged by size: 

                4079     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3905     882 
   Size:        617    2406    2219

  Fragments arranged by size: 

                2406    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3033     493 
   Size:        711    1829    2702

  Fragments arranged by size: 

                2702    1829     711
 
BsaBI GATnn'nnATC

Cuts at:   5236    5236 
   Size:       5242
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2018    3049    3431    4066 
   Size:         53      83     186     936     485    1031     382     635

Cuts at:   4066     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1031     936     635     485     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3675    4073    4347    4494    4905    3675 
   Size:        398     274     147     411    4012

  Fragments arranged by size: 

                4012     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5017

  Fragments arranged by size: 

                5017     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5242
 
BseYI C'CCAG_C

Cuts at:   2187    2400    4445    4941    2187 
   Size:        213    2045     496    2488

  Fragments arranged by size: 

                2488    2045     496     213
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4669
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2692    3453    4481    1540 
   Size:        275     186     691     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     691     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3303    3388    4459    3303 
   Size:         85    1071    4086

  Fragments arranged by size: 

                4086    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2753    2846    2753 
   Size:         93    5149
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2143    2270    2561    3303    3388    4459    1391 
   Size:        399     353     127     291     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     353     291     127      85
            
 
BspEI T'CCGG_A

Cuts at:   4905    4905 
   Size:       5242
 
BspHI T'CATG_A

Cuts at:   3131    4861    3131 
   Size:       1730    3512
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2597     844 
   Size:       1753    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2533    3129    1394 
   Size:        429     710     596    3507

  Fragments arranged by size: 

                3507     710     596     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2349    3737    3911      65 
   Size:       1183    1101    1388     174    1396

  Fragments arranged by size: 

                1396    1388    1183    1101     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3886    4073    1700 
   Size:        110    2076     187    2869

  Fragments arranged by size: 

                2869    2076     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5242
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5242
 
BssSI C'ACGA_G

Cuts at:   3302    4314    3302 
   Size:       1012    4230
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5242
 
BstBI TT'CG_AA

Cuts at:   4046    4046 
   Size:       5242
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5242
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2505    2505 
   Size:       5242
 
BstYI r'GATC_y

Cuts at:   1972    2572    2939    3324    3341    4793    4879    4891    5237 
   Size:        600     367     385      17    1452      86      12     346

Cuts at:   5237    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     600     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2538     513 
   Size:        552     939     534    3217

  Fragments arranged by size: 

                3217     939     552     534
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4503

  Fragments arranged by size: 

                4503     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2758    3552    3572    2758 
   Size:        794      20    4428

  Fragments arranged by size: 

                4428     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5242
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5207
 
DraI TTT'AAA

Cuts at:   2546    2546 
   Size:       5242
 
DraIII CAC_nnn'GTG

Cuts at:   2444    2444 
   Size:       5242
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4249     809 
   Size:        507    2933    1802

  Fragments arranged by size: 

                2933    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2689    3578    5207 
   Size:         54    1425      50     275      18     859     889    1629

Cuts at:   5207       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     859     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2689    1537 
   Size:        275     877    4090

  Fragments arranged by size: 

                4090     877     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3172    1353 
   Size:       1819    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3829    4203    4349     387 
   Size:       1064     138    2240     374     146    1280

  Fragments arranged by size: 

                2240    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2216    2478    3305    3902    4689    4957    1239 
   Size:        393     584     262     827     597     787     268    1524

  Fragments arranged by size: 

                1524     827     787     597     584     393     268     262
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2024    2299    2024 
   Size:        275    4967
 
EcoRI G'AATT_C

Cuts at:   2213    2551    2929    2213 
   Size:        338     378    4526

  Fragments arranged by size: 

                4526     378     338
 
FspI TGC'GCA

Cuts at:   3748    3748 
   Size:       5242
 
HaeII r_GCGC'y

Cuts at:   1678    2075    4389    1678 
   Size:        397    2314    2531

  Fragments arranged by size: 

                2531    2314     397
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3883    3915    3957    3989    1373 
   Size:         32     135      32    2311      32      42      32    2626

  Fragments arranged by size: 

                2626    2311     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2586    2832     669 
   Size:        988     281     648     246    3079

  Fragments arranged by size: 

                3079     988     648     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2460     748 
   Size:       1712    3530
 
HpaI GTT'AAC

Cuts at:   2832    2832 
   Size:       5242
 
KpnI G_GTAC'C

Cuts at:   2224    2567    2224 
   Size:        343    4899
 
MfeI C'AATT_G

Cuts at:   2841    2841 
   Size:       5242
 
MluI A'CGCG_T

Cuts at:   5118    5118 
   Size:       5242
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4356    4540    1305 
   Size:       3051     184    2007

  Fragments arranged by size: 

                3051    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3420

  Fragments arranged by size: 

                3420    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2160    2503    3200    3559    3718     518 
   Size:        648     994     343     697     359     159    2042

  Fragments arranged by size: 

                2042     994     697     648     359     343     159
 
MspA1I CmG'CkG

Cuts at:   1606    2191    2422    2658    3335    4483    4728    1606 
   Size:        585     231     236     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     585     245     236     231
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2538     513 
   Size:        552     939     534    3217

  Fragments arranged by size: 

                3217     939     552     534
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5242
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5242
 
NotI GC'GGCC_GC

Cuts at:   2689    2689 
   Size:       5242
 
NspI r_CATG'y

Cuts at:   1132    1229    2600    4145    1132 
   Size:         97    1371    1545    2229

  Fragments arranged by size: 

                2229    1545    1371      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5242
 
PciI A'CATG_T

Cuts at:   4141    4141 
   Size:       5242
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2444    1108 
   Size:       1336    3906
 
PmeI GTTT'AAAC

Cuts at:   2546    2546 
   Size:       5242
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3311    3343    4824    4856    3311 
   Size:         32    1481      32    3697

  Fragments arranged by size: 

                3697    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2024    2299    2024 
   Size:        275    4967
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5242
 
PsiI TTA'TAA

Cuts at:   2064    2812    2064 
   Size:        748    4494
 
PspXI vC'TCGA_Gb

Cuts at:   2084    2084 
   Size:       5242
 
PstI C_TGCA'G

Cuts at:    830    2427    2594     830 
   Size:       1597     167    3478

  Fragments arranged by size: 

                3478    1597     167
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5242
 
PvuII CAG'CTG

Cuts at:   2191    2422    2191 
   Size:        231    5011
 
SalI G'TCGA_C

Cuts at:   1936    2584    1936 
   Size:        648    4594
 
SanDI GG'GwC_CC

Cuts at:   2024    2024 
   Size:       5242
 
SbfI CC_TGCA'GG

Cuts at:   2594    2594 
   Size:       5242
 
ScaI AGT'ACT

Cuts at:   3490    5202    3490 
   Size:       1712    3530
 
SexAI A'CCwGG_T

Cuts at:   2440    2440 
   Size:       5242
 
SfcI C'TryA_G

Cuts at:    826    1045    2423    2590    3725    4406    4597     826 
   Size:        219    1378     167    1135     681     191    1471

  Fragments arranged by size: 

                1471    1378    1135     681     219     191     167
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5242
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5242
 
SmaI CCC'GGG

Cuts at:   1872    2528    2569    1872 
   Size:        656      41    4545

  Fragments arranged by size: 

                4545     656      41
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2084    3346    4247    4509     820 
   Size:        197     926     141    1262     901     262    1553

  Fragments arranged by size: 

                1553    1262     926     901     262     197     141
 
SnaBI TAC'GTA

Cuts at:    493    3033     493 
   Size:       2540    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5242
 
SphI G_CATG'C

Cuts at:   1132    2600    1132 
   Size:       1468    3774
 
SspI AAT'ATT

Cuts at:      5      52    3166       5 
   Size:         47    3114    2081

  Fragments arranged by size: 

                3114    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5242
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2538    2681     513 
   Size:        552     939     534     143    3074

  Fragments arranged by size: 

                3074     939     552     534     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2201    3269    3286    3439    3624    4069     325 
   Size:       1255     621    1068      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1068     621     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3488    5200      96 
   Size:        274      80      33      51    1443    1511    1712     138

  Fragments arranged by size: 

                1712    1511    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5202    5234    5202 
   Size:         32    5210
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5242
 
XbaI T'CTAG_A

Cuts at:   2578    2578 
   Size:       5242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    2011 
   Size:       5242
 
XhoI C'TCGA_G

Cuts at:   1943    2084    1943 
   Size:        141    5101
 
XmaI C'CCGG_G

Cuts at:   1870    2526    2567    1870 
   Size:        656      41    4545

  Fragments arranged by size: 

                4545     656      41
 
XmnI GAAnn'nnTTC

Cuts at:   3371    3371 
   Size:       5242
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3050     276 
   Size:         53      83     186     936    1516    2468

  Fragments arranged by size: 

                2468    1516     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PstI     PvuI
    PvuII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
     PsrI    RsrII     SacI    SacII     SapI     SfiI     SfoI     SrfI
     SwaI
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