(Circular) (Six-base) MAPSORT of: pFN21AE0030.seq Check: 6628 from: 1 to: 6361 pFN21AE0030 6361 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2943 2943 Size: 6361 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2340 4171 278 Size: 53 83 186 936 804 1831 2468 Fragments arranged by size: 2468 1831 936 804 186 83 53 AccI GT'mk_AC Cuts at: 1937 3704 1937 Size: 1767 4594 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 4488 4861 1563 Size: 2925 373 3063 Fragments arranged by size: 3063 2925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2508 2858 3452 3521 3592 4424 5808 Size: 398 478 350 594 69 71 832 1384 Cuts at: 5808 6076 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 832 594 478 398 350 268 71 69 AfeI AGC'GCT Cuts at: 2831 3092 2831 Size: 261 6100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 2940 5260 6237 1203 Size: 1737 2320 977 1327 Fragments arranged by size: 2320 1737 1327 977 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 3178 5090 3178 Size: 1912 4449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2945 1184 Size: 1761 4600 AlwNI CAG_nnn'CTG Cuts at: 3637 4104 5676 3637 Size: 467 1572 4322 Fragments arranged by size: 4322 1572 467 ApaLI G'TGCA_C Cuts at: 3041 4418 5574 3041 Size: 1377 1156 3828 Fragments arranged by size: 3828 1377 1156 ApoI r'AATT_y Cuts at: 1493 3670 3896 4048 6157 1493 Size: 2177 226 152 2109 1697 Fragments arranged by size: 2177 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 2955 3183 3240 3686 1870 Size: 73 1012 228 57 446 4545 Fragments arranged by size: 4545 1012 446 228 73 57 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 2288 2963 3682 5137 Size: 325 217 627 284 217 675 719 1455 Cuts at: 5137 618 Size: 1842 Fragments arranged by size: 1842 1455 719 675 627 325 284 217 217 BanII G_rGCy'C Cuts at: 1391 2474 3680 1391 Size: 1083 1206 4072 Fragments arranged by size: 4072 1206 1083 BbeI G_GCGC'C Cuts at: 2075 2292 2075 Size: 217 6144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2035 2847 3478 928 Size: 971 136 812 631 3811 Fragments arranged by size: 3811 971 812 631 136 BbvCI CC'TCA_GC Cuts at: 3398 3398 Size: 6361 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4552 4586 1903 Size: 34 50 34 2531 34 3678 Fragments arranged by size: 3678 2531 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4253 5463 4253 Size: 1210 5151 BclI T'GATC_A Cuts at: 1548 1548 Size: 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3593 4972 136 Size: 107 122 71 631 605 1921 1379 1525 Fragments arranged by size: 1921 1525 1379 631 605 122 107 71 BglII A'GATC_T Cuts at: 6356 6356 Size: 6361 BlpI GC'TnA_GC Cuts at: 2210 3778 2210 Size: 1568 4793 Bme1580I G_kGCm'C Cuts at: 1790 2331 2966 3045 4422 5578 1790 Size: 541 635 79 1377 1156 2573 Fragments arranged by size: 2573 1377 1156 635 541 79 BmgBI CAC'GTC Cuts at: 1318 3070 3175 3439 3454 1318 Size: 1752 105 264 15 4225 Fragments arranged by size: 4225 1752 264 105 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4092 5050 442 Size: 416 53 83 399 24 2675 958 1753 Fragments arranged by size: 2675 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2738 2817 2848 3014 3155 3428 5021 1239 Size: 1499 79 31 166 141 273 1593 2579 Fragments arranged by size: 2579 1593 1499 273 166 141 79 31 Bpu10I CC'TnA_GC Cuts at: 3398 3419 3398 Size: 21 6340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4486 5351 5649 4486 Size: 865 298 5198 Fragments arranged by size: 5198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2378 5024 882 Size: 617 879 2646 2219 Fragments arranged by size: 2646 2219 879 617 BsaAI yAC'GTr Cuts at: 493 1204 2941 3592 4152 493 Size: 711 1737 651 560 2702 Fragments arranged by size: 2702 1737 711 651 560 BsaBI GATnn'nnATC Cuts at: 3308 6355 3308 Size: 3047 3314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2072 2094 2289 Size: 53 83 186 936 482 57 22 195 Cuts at: 2289 2337 2649 2703 4168 4550 5185 275 Size: 48 312 54 1465 382 635 1451 Fragments arranged by size: 1465 1451 936 635 482 382 312 195 186 83 57 54 53 48 22 BsaWI w'CCGG_w Cuts at: 4794 5192 5466 5613 6024 4794 Size: 398 274 147 411 5131 Fragments arranged by size: 5131 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2846 2876 1178 Size: 30 165 30 1443 30 4663 Fragments arranged by size: 4663 1443 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2814 3605 1186 Size: 1628 791 3942 Fragments arranged by size: 3942 1628 791 BseYI C'CCAG_C Cuts at: 2602 2677 5564 6060 2602 Size: 75 2887 496 2903 Fragments arranged by size: 2903 2887 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 2921 1163 Size: 573 1179 6 4603 Fragments arranged by size: 4603 1179 573 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3537 3811 4572 5600 1540 Size: 275 186 1536 274 761 1028 2301 Fragments arranged by size: 2301 1536 1028 761 275 274 186 BsiHKAI G_wGCw'C Cuts at: 2229 3045 3087 3414 4422 4507 5578 2229 Size: 816 42 327 1008 85 1071 3012 Fragments arranged by size: 3012 1071 1008 816 327 85 42 BsmI GAATG_Cn' Cuts at: 2170 3520 3872 3965 2170 Size: 1350 352 93 4566 Fragments arranged by size: 4566 1350 352 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2710 2990 2710 Size: 280 6081 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2331 2474 2966 3045 3087 3414 Size: 399 439 102 143 492 79 42 327 Cuts at: 3414 3680 4422 4507 5578 1391 Size: 266 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 439 399 327 266 143 102 85 79 42 BspEI T'CCGG_A Cuts at: 6024 6024 Size: 6361 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 2943 3555 3716 844 Size: 1301 798 612 161 3489 Fragments arranged by size: 3489 1301 798 612 161 BsrBI CCG'CTC Cuts at: 1394 1823 2700 4248 1394 Size: 429 877 1548 3507 Fragments arranged by size: 3507 1548 877 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3222 3564 4856 5030 65 Size: 1183 1974 342 1292 174 1396 Fragments arranged by size: 1974 1396 1292 1183 342 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3286 3532 5005 5192 1700 Size: 110 1476 246 1473 187 2869 Fragments arranged by size: 2869 1476 1473 246 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6361 BssHII G'CGCG_C Cuts at: 1925 2186 1925 Size: 261 6100 BssSI C'ACGA_G Cuts at: 2102 3086 3214 3641 4421 5433 2102 Size: 984 128 427 780 1012 3030 Fragments arranged by size: 3030 1012 984 780 427 128 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6361 BstBI TT'CG_AA Cuts at: 5165 5165 Size: 6361 BstEII G'GTnAC_C Cuts at: 1184 2530 1184 Size: 1346 5015 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2317 Size: 6361 BstYI r'GATC_y Cuts at: 1972 3309 3691 4058 4443 4460 5912 5998 6010 Size: 1337 382 367 385 17 1452 86 12 Cuts at: 6010 6356 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1337 385 382 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3021 3021 Size: 6361 BtgI C'CryG_G Cuts at: 513 1065 2292 2316 2379 2409 2910 3036 3359 Size: 552 1227 24 63 30 501 126 323 Cuts at: 3359 513 Size: 3515 Fragments arranged by size: 3515 1227 552 501 323 126 63 30 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5622 Fragments arranged by size: 5622 683 56 BtsI GCAGTG_nn' Cuts at: 2175 2508 3877 4671 4691 2175 Size: 333 1369 794 20 3845 Fragments arranged by size: 3845 1369 794 333 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6326 DraI TTT'AAA Cuts at: 3665 3665 Size: 6361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5368 809 Size: 507 4052 1802 Fragments arranged by size: 4052 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2376 2505 Size: 54 1425 50 275 18 489 57 129 Cuts at: 2505 3003 3534 3808 4697 6326 8 Size: 498 531 274 889 1629 43 Fragments arranged by size: 1629 1425 889 531 498 489 275 274 129 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3534 3808 1537 Size: 275 1722 274 4090 Fragments arranged by size: 4090 1722 275 274 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 4291 1353 Size: 1385 1553 3423 Fragments arranged by size: 3423 1553 1385 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 2449 2464 2622 3077 3092 Size: 1064 138 627 233 15 158 455 15 Cuts at: 3092 4948 5322 5468 387 Size: 1856 374 146 1280 Fragments arranged by size: 1856 1280 1064 627 455 374 233 158 146 138 15 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2508 2738 2817 2848 2858 3014 Size: 393 398 478 230 79 31 10 156 Cuts at: 3014 3155 3428 3452 3521 3592 4424 5021 5808 Size: 141 273 24 69 71 832 597 787 Cuts at: 5808 6076 1239 Size: 268 1524 Fragments arranged by size: 1524 832 787 597 478 398 393 273 268 230 156 141 79 71 69 31 24 10 EcoNI CCTnn'n_nnAGG Cuts at: 2873 3062 2873 Size: 189 6172 EcoO109I rG'GnC_Cy Cuts at: 2269 2422 2614 3232 3472 2269 Size: 153 192 618 240 5158 Fragments arranged by size: 5158 618 240 192 153 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 FspI TGC'GCA Cuts at: 2501 4867 2501 Size: 2366 3995 HaeII r_GCGC'y Cuts at: 1678 2075 2292 2833 3094 5508 1678 Size: 397 217 541 261 2414 2531 Fragments arranged by size: 2531 2414 541 397 261 217 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3164 3196 5002 5034 5076 Size: 32 135 32 1592 32 1806 32 42 Cuts at: 5076 5108 1373 Size: 32 2626 Fragments arranged by size: 2626 1806 1592 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3705 3951 669 Size: 988 281 1767 246 3079 Fragments arranged by size: 3079 1767 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6361 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KasI G'GCGC_C Cuts at: 2071 2288 2071 Size: 217 6144 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 3960 3960 Size: 6361 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2715 5475 5659 1305 Size: 1410 2760 184 2007 Fragments arranged by size: 2760 2007 1410 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3993 Fragments arranged by size: 3993 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3173 3287 4319 4678 4837 518 Size: 648 2007 114 1032 359 159 2042 Fragments arranged by size: 2042 2007 1032 648 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2054 2080 2220 2538 3325 3361 3373 3499 Size: 448 26 140 318 787 36 12 126 Cuts at: 3499 3777 4454 5602 5847 1606 Size: 278 677 1148 245 2120 Fragments arranged by size: 2120 1148 787 677 448 318 278 245 140 126 36 26 12 NaeI GCC'GGC Cuts at: 3534 3534 Size: 6361 NarI GG'CG_CC Cuts at: 2072 2289 2072 Size: 217 6144 NcoI C'CATG_G Cuts at: 513 1065 2316 2379 3036 513 Size: 552 1251 63 657 3838 Fragments arranged by size: 3838 1251 657 552 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NgoMIV G'CCGG_C Cuts at: 3532 3532 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NspI r_CATG'y Cuts at: 1132 1229 2125 3286 3719 5264 1132 Size: 97 896 1161 433 1545 2229 Fragments arranged by size: 2229 1545 1161 896 433 97 PasI CC'CwG_GG Cuts at: 1348 2310 2467 1348 Size: 962 157 5242 Fragments arranged by size: 5242 962 157 PciI A'CATG_T Cuts at: 5260 5260 Size: 6361 PflMI CCAn_nnn'nTGG Cuts at: 1108 2832 1108 Size: 1724 4637 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PmlI CAC'GTG Cuts at: 2941 2941 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3014 3046 4430 4462 5943 5975 3014 Size: 32 1384 32 1481 32 3400 Fragments arranged by size: 3400 1481 1384 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2614 3472 2614 Size: 858 5503 PshAI GACnn'nnGTC Cuts at: 1613 2390 3242 1613 Size: 777 852 4732 Fragments arranged by size: 4732 852 777 PsiI TTA'TAA Cuts at: 3931 3931 Size: 6361 PspXI vC'TCGA_Gb Cuts at: 3240 3240 Size: 6361 PstI C_TGCA'G Cuts at: 830 2142 2973 3713 830 Size: 1312 831 740 3478 Fragments arranged by size: 3478 1312 831 740 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 3325 3373 3499 3325 Size: 48 126 6187 Fragments arranged by size: 6187 126 48 SacII CC_GC'GG Cuts at: 3362 3362 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SanDI GG'GwC_CC Cuts at: 3472 3472 Size: 6361 SbfI CC_TGCA'GG Cuts at: 2142 3713 2142 Size: 1571 4790 ScaI AGT'ACT Cuts at: 2493 4609 6321 2493 Size: 2116 1712 2533 Fragments arranged by size: 2533 2116 1712 SfcI C'TryA_G Cuts at: 826 1045 2138 2969 3709 4844 5525 5716 826 Size: 219 1093 831 740 1135 681 191 1471 Fragments arranged by size: 1471 1135 1093 831 740 681 219 191 SfoI GGC'GCC Cuts at: 2073 2290 2073 Size: 217 6144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3185 3688 1872 Size: 1313 503 4545 Fragments arranged by size: 4545 1313 503 SmlI C'TyrA_G Cuts at: 820 1017 1943 3240 4465 5366 5628 820 Size: 197 926 1297 1225 901 262 1553 Fragments arranged by size: 1553 1297 1225 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 2125 3719 1132 Size: 993 1594 3774 Fragments arranged by size: 3774 1594 993 SspI AAT'ATT Cuts at: 5 52 4285 5 Size: 47 4233 2081 Fragments arranged by size: 4233 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6361 StyI C'CwwG_G Cuts at: 513 1065 2316 2379 3036 3800 513 Size: 552 1251 63 657 764 3074 Fragments arranged by size: 3074 1251 764 657 552 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4388 4405 4558 4743 5188 325 Size: 1255 2808 17 153 185 445 1498 Fragments arranged by size: 2808 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2491 4607 Size: 274 80 33 51 1443 423 91 2116 Cuts at: 4607 6319 96 Size: 1712 138 Fragments arranged by size: 2116 1712 1443 423 274 138 91 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2846 2878 6321 6353 2846 Size: 32 3443 32 2854 Fragments arranged by size: 3443 2854 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2339 1316 Size: 1023 5338 XbaI T'CTAG_A Cuts at: 3697 3697 Size: 6361 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2632 2833 2632 Size: 201 6160 XhoI C'TCGA_G Cuts at: 1943 3240 1943 Size: 1297 5064 XmaI C'CCGG_G Cuts at: 1870 3183 3686 1870 Size: 1313 503 4545 Fragments arranged by size: 4545 1313 503 XmnI GAAnn'nnTTC Cuts at: 4490 4490 Size: 6361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2338 4169 276 Size: 53 83 186 936 804 1831 2468 Fragments arranged by size: 2468 1831 936 804 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI