(Circular) (Six-base) MAPSORT of: pFN21AE0343.seq Check: 6551 from: 1 to: 6520 pFN21AE0343 6520 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2202 2238 3863 1937 Size: 265 36 1625 4594 Fragments arranged by size: 4594 1625 265 36 Acc65I G'GTAC_C Cuts at: 3099 3841 3099 Size: 742 5778 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4583 5967 6235 1632 Size: 2951 1384 268 1917 Fragments arranged by size: 2951 1917 1384 268 AfeI AGC'GCT Cuts at: 3782 3782 Size: 6520 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 2550 3190 5419 6396 1203 Size: 1347 640 2229 977 1327 Fragments arranged by size: 2229 1347 1327 977 640 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 3742 5249 3742 Size: 1507 5013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2883 2886 1184 Size: 1699 3 4818 Fragments arranged by size: 4818 1699 3 AlwNI CAG_nnn'CTG Cuts at: 2921 2962 3253 3495 3517 4263 5835 2921 Size: 41 291 242 22 746 1572 3606 Fragments arranged by size: 3606 1572 746 291 242 41 22 ApaI G_GGCC'C Cuts at: 2914 2914 Size: 6520 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 3829 4055 4207 6316 1493 Size: 2336 226 152 2109 1697 Fragments arranged by size: 2336 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5074 160 Size: 872 4042 1606 Fragments arranged by size: 4042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 3344 3716 3845 1870 Size: 73 1401 372 129 4545 Fragments arranged by size: 4545 1401 372 129 73 BamHI G'GATC_C Cuts at: 3850 4217 3850 Size: 367 6153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3099 3841 5296 618 Size: 325 217 627 1312 742 1455 1842 Fragments arranged by size: 1842 1455 1312 742 627 325 217 BanII G_rGCy'C Cuts at: 1391 2192 2702 2841 2858 2914 3345 3435 3839 Size: 801 510 139 17 56 431 90 404 Cuts at: 3839 1391 Size: 4072 Fragments arranged by size: 4072 801 510 431 404 139 90 56 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3300 928 Size: 971 1401 4148 Fragments arranged by size: 4148 1401 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 2410 1548 Size: 862 5658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2462 2825 5131 Size: 107 122 71 631 605 790 363 2306 Cuts at: 5131 136 Size: 1525 Fragments arranged by size: 2306 1525 790 631 605 363 122 107 71 BglII A'GATC_T Cuts at: 2121 6515 2121 Size: 4394 2126 BlpI GC'TnA_GC Cuts at: 3243 3476 3937 3243 Size: 233 461 5826 Fragments arranged by size: 5826 461 233 Bme1580I G_kGCm'C Cuts at: 1790 2914 3024 3546 4581 5737 1790 Size: 1124 110 522 1035 1156 2573 Fragments arranged by size: 2573 1156 1124 1035 522 110 BmgBI CAC'GTC Cuts at: 1318 2014 2080 1318 Size: 696 66 5758 Fragments arranged by size: 5758 696 66 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 2415 3505 Size: 416 53 83 399 24 660 338 1090 Cuts at: 3505 4251 5209 442 Size: 746 958 1753 Fragments arranged by size: 1753 1090 958 746 660 416 399 338 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2727 2855 2928 3299 3506 3743 5180 Size: 887 601 128 73 371 207 237 1437 Cuts at: 5180 1239 Size: 2579 Fragments arranged by size: 2579 1437 887 601 371 237 207 128 73 Bpu10I CC'TnA_GC Cuts at: 3398 3398 Size: 6520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 4645 5510 5808 2387 Size: 2258 865 298 3099 Fragments arranged by size: 3099 2258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3583 5183 882 Size: 617 2084 1600 2219 Fragments arranged by size: 2219 2084 1600 617 BsaAI yAC'GTr Cuts at: 493 1204 2553 3193 4311 493 Size: 711 1349 640 1118 2702 Fragments arranged by size: 2702 1349 1118 711 640 BsaBI GATnn'nnATC Cuts at: 6514 6514 Size: 6520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2417 4327 4709 5344 Size: 53 83 186 936 884 1910 382 635 Cuts at: 5344 275 Size: 1451 Fragments arranged by size: 1910 1451 936 884 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3216 4953 5351 5625 5772 6183 3216 Size: 1737 398 274 147 411 3553 Fragments arranged by size: 3553 1737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3164 3194 1178 Size: 30 165 30 1761 30 4504 Fragments arranged by size: 4504 1761 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2681 2804 3050 3244 3281 3344 3422 Size: 937 558 123 246 194 37 63 78 Cuts at: 3422 1186 Size: 4284 Fragments arranged by size: 4284 937 558 246 194 123 78 63 37 BseYI C'CCAG_C Cuts at: 2088 2092 2707 3591 3708 3813 5723 6219 2088 Size: 4 615 884 117 105 1910 496 2389 Fragments arranged by size: 2389 1910 884 615 496 117 105 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2713 3530 1163 Size: 573 579 398 817 4153 Fragments arranged by size: 4153 817 579 573 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3970 4731 5759 1540 Size: 275 186 1969 761 1028 2301 Fragments arranged by size: 2301 1969 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 4581 4666 5737 3435 Size: 1146 85 1071 4218 Fragments arranged by size: 4218 1146 1071 85 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2702 2841 2858 2914 3024 3345 Size: 399 402 510 139 17 56 110 321 Cuts at: 3345 3435 3546 3839 4581 4666 5737 1391 Size: 90 111 293 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 402 399 321 293 139 111 110 90 85 56 17 BspEI T'CCGG_A Cuts at: 3216 6183 3216 Size: 2967 3553 BspHI T'CATG_A Cuts at: 4409 6139 4409 Size: 1730 4790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3875 844 Size: 3031 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2831 2902 4407 1394 Size: 429 1008 71 1505 3507 Fragments arranged by size: 3507 1505 1008 429 71 BsrDI GCAATG_nn' Cuts at: 65 1248 5015 5189 65 Size: 1183 3767 174 1396 Fragments arranged by size: 3767 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3637 5164 5351 1700 Size: 110 1827 1527 187 2869 Fragments arranged by size: 2869 1827 1527 187 110 BsrGI T'GTAC_A Cuts at: 96 2499 96 Size: 2403 4117 BssHII G'CGCG_C Cuts at: 1925 2021 1925 Size: 96 6424 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 3122 3253 3517 3541 794 Size: 2328 131 264 24 3773 Fragments arranged by size: 3773 2328 264 131 24 BstBI TT'CG_AA Cuts at: 3418 5324 3418 Size: 1906 4614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6520 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2952 3563 2458 Size: 494 611 5415 Fragments arranged by size: 5415 611 494 BstYI r'GATC_y Cuts at: 1972 2121 2398 3850 4217 4602 4619 6071 6157 Size: 149 277 1452 367 385 17 1452 86 Cuts at: 6157 6169 6515 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1452 385 367 346 277 149 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2808 3327 2808 Size: 519 6001 BtgI C'CryG_G Cuts at: 513 1065 2004 2451 2607 3732 3807 513 Size: 552 939 447 156 1125 75 3226 Fragments arranged by size: 3226 1125 939 552 447 156 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3424 487 Size: 683 56 2198 3583 Fragments arranged by size: 3583 2198 683 56 BtsI GCAGTG_nn' Cuts at: 3254 3390 4036 4830 4850 3254 Size: 136 646 794 20 4924 Fragments arranged by size: 4924 794 646 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3173 3575 5527 809 Size: 507 1857 402 1952 1802 Fragments arranged by size: 1952 1857 1802 507 402 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2523 3631 3681 Size: 54 1425 50 275 18 693 1108 50 Cuts at: 3681 3729 3967 4856 6485 8 Size: 48 238 889 1629 43 Fragments arranged by size: 1629 1425 1108 889 693 275 238 54 50 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3967 1537 Size: 275 2155 4090 Fragments arranged by size: 4090 2155 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2293 3440 4450 1353 Size: 940 1147 1010 3423 Fragments arranged by size: 3423 1147 1010 940 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5107 5481 5627 387 Size: 1064 138 3518 374 146 1280 Fragments arranged by size: 3518 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3433 3433 Size: 6520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2727 2855 2928 3299 3506 3743 Size: 393 494 601 128 73 371 207 237 Cuts at: 3743 4583 5180 5967 6235 1239 Size: 840 597 787 268 1524 Fragments arranged by size: 1524 840 787 601 597 494 393 371 268 237 207 128 73 EcoO109I rG'GnC_Cy Cuts at: 2245 2332 2428 2518 2574 2640 2735 2910 2911 Size: 87 96 90 56 66 95 175 1 Cuts at: 2911 3175 2245 Size: 264 5590 Fragments arranged by size: 5590 264 175 96 95 90 87 66 56 1 EcoRI G'AATT_C Cuts at: 3829 4207 3829 Size: 378 6142 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 3072 3784 5667 1678 Size: 1394 712 1883 2531 Fragments arranged by size: 2531 1883 1394 712 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2345 2377 2585 Size: 32 135 32 671 32 70 32 208 Cuts at: 2585 2617 2909 2941 3728 3760 5161 5193 5235 Size: 32 292 32 787 32 1401 32 42 Cuts at: 5235 5267 1373 Size: 32 2626 Fragments arranged by size: 2626 1401 787 671 292 208 135 70 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3864 4110 669 Size: 988 281 1926 246 3079 Fragments arranged by size: 3079 1926 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6520 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 3103 3845 3103 Size: 742 5778 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 3186 5634 5818 1305 Size: 1260 621 2448 184 2007 Fragments arranged by size: 2448 2007 1260 621 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2525 3633 3683 10 Size: 54 1425 343 693 1108 50 2847 Fragments arranged by size: 2847 1425 1108 693 343 54 50 MslI CAynn'nnrTG Cuts at: 518 1166 3561 4478 4837 4996 518 Size: 648 2395 917 359 159 2042 Fragments arranged by size: 2395 2042 917 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2618 3371 3574 3679 3936 4613 5761 Size: 486 526 753 203 105 257 677 1148 Cuts at: 5761 6006 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 753 677 526 486 257 245 203 105 NaeI GCC'GGC Cuts at: 3639 3639 Size: 6520 NcoI C'CATG_G Cuts at: 513 1065 2004 2451 2607 3807 513 Size: 552 939 447 156 1200 3226 Fragments arranged by size: 3226 1200 939 552 447 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NgoMIV G'CCGG_C Cuts at: 3637 3637 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 2602 2893 3542 3878 5423 1132 Size: 97 1373 291 649 336 1545 2229 Fragments arranged by size: 2229 1545 1373 649 336 291 97 PasI CC'CwG_GG Cuts at: 1348 2761 3360 1348 Size: 1413 599 4508 Fragments arranged by size: 4508 1413 599 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 2067 1108 Size: 959 5561 PfoI T'CCnGG_A Cuts at: 2384 2849 3462 3522 2384 Size: 465 613 60 5382 Fragments arranged by size: 5382 613 465 60 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PmlI CAC'GTG Cuts at: 2553 3193 2553 Size: 640 5880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2339 2371 4589 4621 6102 6134 2339 Size: 32 2218 32 1481 32 2725 Fragments arranged by size: 2725 2218 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2332 2428 2518 2640 3175 2332 Size: 96 90 122 535 5677 Fragments arranged by size: 5677 535 122 96 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6520 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PspOMI G'GGCC_C Cuts at: 2910 2910 Size: 6520 PstI C_TGCA'G Cuts at: 830 3084 3228 3265 3678 3872 830 Size: 2254 144 37 413 194 3478 Fragments arranged by size: 3478 2254 413 194 144 37 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2092 3574 3679 2092 Size: 1482 105 4933 Fragments arranged by size: 4933 1482 105 SacI G_AGCT'C Cuts at: 3435 3435 Size: 6520 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SanDI GG'GwC_CC Cuts at: 2332 2640 2332 Size: 308 6212 SapI GCTCTTCn'nnn_ Cuts at: 3440 3440 Size: 6520 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 2203 3080 3224 3261 3674 3868 5003 Size: 219 1158 877 144 37 413 194 1135 Cuts at: 5003 5684 5875 826 Size: 681 191 1471 Fragments arranged by size: 1471 1158 1135 877 681 413 219 194 191 144 37 SfiI GGCCn_nnn'nGGCC Cuts at: 2462 2825 2462 Size: 363 6157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 3718 3847 1872 Size: 1846 129 4545 Fragments arranged by size: 4545 1846 129 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 4624 5525 5787 820 Size: 197 926 459 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SrfI GCCC'GGGC Cuts at: 3718 3718 Size: 6520 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 2813 3010 1517 Size: 1296 197 5027 Fragments arranged by size: 5027 1296 197 StyI C'CwwG_G Cuts at: 513 1065 2004 2451 2607 2823 2841 2979 3807 Size: 552 939 447 156 216 18 138 828 Cuts at: 3807 3959 513 Size: 152 3074 Fragments arranged by size: 3074 939 828 552 447 216 156 152 138 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2753 4547 4564 4717 4902 5347 325 Size: 1255 1173 1794 17 153 185 445 1498 Fragments arranged by size: 1794 1498 1255 1173 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2499 3180 3333 Size: 274 80 33 51 1443 522 681 153 Cuts at: 3333 4766 6478 96 Size: 1433 1712 138 Fragments arranged by size: 1712 1443 1433 681 522 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2542 2574 6480 6512 2542 Size: 32 3906 32 2550 Fragments arranged by size: 3906 2550 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6520 XbaI T'CTAG_A Cuts at: 3319 3856 3319 Size: 537 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2458 2830 2011 Size: 447 372 5701 Fragments arranged by size: 5701 447 372 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 3716 3845 1870 Size: 1846 129 4545 Fragments arranged by size: 4545 1846 129 XmnI GAAnn'nnTTC Cuts at: 2354 2537 4649 2354 Size: 183 2112 4225 Fragments arranged by size: 4225 2112 183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfoI SwaI