Restriction map for
pFN21AE0037
(Linear) (Six-base) MAPSORT of: KIEE0037.seq Check: 7264 from: 1 to: 2220
KIEE0037.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 1010 2220
Size: 1010 1210
Acc65I G'GTAC_C
Cuts at: 0 710 2220
Size: 710 1510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1592 1668 2150 2220
Size: 1210 382 76 482 70
Fragments arranged by size:
1210 482 382 76 70
AfeI AGC'GCT
Cuts at: 0 256 2220
Size: 256 1964
AhdI GACnn_n'nnGTC
Cuts at: 0 270 2220
Size: 270 1950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 173 951 1813 2220
Size: 173 778 862 407
Fragments arranged by size:
862 778 407 173
AlwNI CAG_nnn'CTG
Cuts at: 0 117 246 655 1432 1894 2122 2220
Size: 117 129 409 777 462 228 98
Fragments arranged by size:
777 462 409 228 129 117 98
ApaI G_GGCC'C
Cuts at: 0 364 1827 2220
Size: 364 1463 393
Fragments arranged by size:
1463 393 364
ApaLI G'TGCA_C
Cuts at: 0 1117 2220
Size: 1117 1103
ApoI r'AATT_y
Cuts at: 0 1273 2220
Size: 1273 947
AvaI C'yCGr_G
Cuts at: 0 221 1677 2079 2220
Size: 221 1456 402 141
Fragments arranged by size:
1456 402 221 141
BamHI G'GATC_C
Cuts at: 0 167 1460 2220
Size: 167 1293 760
Fragments arranged by size:
1293 760 167
BanI G'GyrC_C
Cuts at: 0 556 710 1144 1198 2049 2220
Size: 556 154 434 54 851 171
Fragments arranged by size:
851 556 434 171 154 54
BanII G_rGCy'C
Cuts at: 0 364 682 1166 1259 1827 2065 2220
Size: 364 318 484 93 568 238 155
Fragments arranged by size:
568 484 364 318 238 155 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 521 1389 2220
Size: 521 868 831
Fragments arranged by size:
868 831 521
BbvCI CC'TCA_GC
Cuts at: 0 1027 1244 2141 2220
Size: 1027 217 897 79
Fragments arranged by size:
1027 897 217 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1203 1237 2220
Size: 1203 34 983
Fragments arranged by size:
1203 983 34
BglII A'GATC_T
Cuts at: 0 1815 2220
Size: 1815 405
BlpI GC'TnA_GC
Cuts at: 0 676 2158 2220
Size: 676 1482 62
Fragments arranged by size:
1482 676 62
Bme1580I G_kGCm'C
Cuts at: 0 364 1121 1201 1827 2052 2220
Size: 364 757 80 626 225 168
Fragments arranged by size:
757 626 364 225 168 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 377 940 2220
Size: 377 563 1280
Fragments arranged by size:
1280 563 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 756 898 1437 2220
Size: 756 142 539 783
Fragments arranged by size:
783 756 539 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 154 177 198 1042 1180 1852 2085
Size: 145 9 23 21 844 138 672 233
Cuts at: 2085 2200 2220
Size: 115 20
Fragments arranged by size:
844 672 233 145 138 115 23 21
20 9
Bpu10I CC'TnA_GC
Cuts at: 0 1027 1244 1441 2141 2220
Size: 1027 217 197 700 79
Fragments arranged by size:
1027 700 217 197 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 869 2220
Size: 130 739 1351
Fragments arranged by size:
1351 739 130
BsaAI yAC'GTr
Cuts at: 0 302 1086 2220
Size: 302 784 1134
Fragments arranged by size:
1134 784 302
BsaHI Gr'CG_yC
Cuts at: 0 1206 1375 2220
Size: 1206 169 845
Fragments arranged by size:
1206 845 169
BsaWI w'CCGG_w
Cuts at: 0 392 826 1463 1595 1955 2220
Size: 392 434 637 132 360 265
Fragments arranged by size:
637 434 392 360 265 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 467 497 2220
Size: 196 30 241 30 1723
Fragments arranged by size:
1723 241 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 475 1261 1557 2220
Size: 415 60 786 296 663
Fragments arranged by size:
786 663 415 296 60
BseYI C'CCAG_C
Cuts at: 0 24 30 54 631 909 981 1112 1135
Size: 24 6 24 577 278 72 131 23
Cuts at: 1135 1235 1504 1808 2220
Size: 100 269 304 412
Fragments arranged by size:
577 412 304 278 269 131 100 72
24 24 23 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1387 2220
Size: 259 1128 833
Fragments arranged by size:
1128 833 259
BsiEI CG_ry'CG
Cuts at: 0 290 2220
Size: 290 1930
BsiHKAI G_wGCw'C
Cuts at: 0 682 930 1121 1166 2045 2220
Size: 682 248 191 45 879 175
Fragments arranged by size:
879 682 248 191 175 45
BsiWI C'GTAC_G
Cuts at: 0 302 2220
Size: 302 1918
Bsp1286I G_dGCh'C
Cuts at: 0 364 682 930 1121 1166 1201 1259 1827
Size: 364 318 248 191 45 35 58 568
Cuts at: 1827 2045 2052 2065 2220
Size: 218 7 13 155
Fragments arranged by size:
568 364 318 248 218 191 155 58
45 35 13 7
BspEI T'CCGG_A
Cuts at: 0 826 1955 2220
Size: 826 1129 265
Fragments arranged by size:
1129 826 265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 887 1941 2220
Size: 722 165 1054 279
Fragments arranged by size:
1054 722 279 165
BsrBI CCG'CTC
Cuts at: 0 1421 2220
Size: 1421 799
BsrDI GCAATG_nn'
Cuts at: 0 560 1577 2220
Size: 560 1017 643
Fragments arranged by size:
1017 643 560
BsrGI T'GTAC_A
Cuts at: 0 644 2220
Size: 644 1576
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 1894 2220
Size: 246 1648 326
Fragments arranged by size:
1648 326 246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 348 917 1047 2220
Size: 102 246 569 130 1173
Fragments arranged by size:
1173 569 246 130 102
BstYI r'GATC_y
Cuts at: 0 128 167 1415 1460 1616 1815 2125 2220
Size: 128 39 1248 45 156 199 310 95
Fragments arranged by size:
1248 310 199 156 128 95 45 39
Bsu36I CC'TnA_GG
Cuts at: 0 723 1360 2200 2220
Size: 723 637 840 20
Fragments arranged by size:
840 723 637 20
BtgI C'CryG_G
Cuts at: 0 1059 1268 1571 2220
Size: 1059 209 303 649
Fragments arranged by size:
1059 649 303 209
BtsI GCAGTG_nn'
Cuts at: 0 10 1146 1877 1921 2220
Size: 10 1136 731 44 299
Fragments arranged by size:
1136 731 299 44 10
EaeI y'GGCC_r
Cuts at: 0 69 287 2220
Size: 69 218 1933
Fragments arranged by size:
1933 218 69
EagI C'GGCC_G
Cuts at: 0 287 2220
Size: 287 1933
EarI CTCTTCn'nnn_
Cuts at: 0 801 1041 1277 2220
Size: 801 240 236 943
Fragments arranged by size:
943 801 240 236
EcoICRI GAG'CTC
Cuts at: 0 680 1164 2220
Size: 680 484 1056
Fragments arranged by size:
1056 680 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 154 177 198 1042 1180 1210 1592
Size: 145 9 23 21 844 138 30 382
Cuts at: 1592 1668 1852 2085 2150 2200 2220
Size: 76 184 233 65 50 20
Fragments arranged by size:
844 382 233 184 145 138 76 65
50 30 23 21 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2100 2220
Size: 2100 120
EcoO109I rG'GnC_Cy
Cuts at: 0 114 231 264 361 480 762 952 1181
Size: 114 117 33 97 119 282 190 229
Cuts at: 1181 1429 1823 1824 1855 2220
Size: 248 394 1 31 365
Fragments arranged by size:
394 365 282 248 229 190 119 117
114 97 33 31 1
EcoRI G'AATT_C
Cuts at: 0 1273 2220
Size: 1273 947
HaeII r_GCGC'y
Cuts at: 0 258 2220
Size: 258 1962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 2220
Size: 256 32 1932
Fragments arranged by size:
1932 256 32
HindIII A'AGCT_T
Cuts at: 0 2132 2220
Size: 2132 88
KpnI G_GTAC'C
Cuts at: 0 714 2220
Size: 714 1506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 2220
Size: 1064 1156
MslI CAynn'nnrTG
Cuts at: 0 1327 1339 2167 2220
Size: 1327 12 828 53
Fragments arranged by size:
1327 828 53 12
MspA1I CmG'CkG
Cuts at: 0 153 243 1112 1714 2220
Size: 153 90 869 602 506
Fragments arranged by size:
869 602 506 153 90
NcoI C'CATG_G
Cuts at: 0 1571 2220
Size: 1571 649
PflMI CCAn_nnn'nTGG
Cuts at: 0 117 707 1432 2220
Size: 117 590 725 788
Fragments arranged by size:
788 725 590 117
PfoI T'CCnGG_A
Cuts at: 0 131 170 863 1157 2220
Size: 131 39 693 294 1063
Fragments arranged by size:
1063 693 294 131 39
PpuMI rG'GwC_Cy
Cuts at: 0 114 231 264 762 952 1181 2220
Size: 114 117 33 498 190 229 1039
Fragments arranged by size:
1039 498 229 190 117 114 33
PspOMI G'GGCC_C
Cuts at: 0 360 1823 2220
Size: 360 1463 397
Fragments arranged by size:
1463 397 360
PstI C_TGCA'G
Cuts at: 0 254 1304 1802 1872 1899 1928 1952 2220
Size: 254 1050 498 70 27 29 24 268
Fragments arranged by size:
1050 498 268 254 70 29 27 24
PvuII CAG'CTG
Cuts at: 0 153 243 1112 2220
Size: 153 90 869 1108
Fragments arranged by size:
1108 869 153 90
SacI G_AGCT'C
Cuts at: 0 682 1166 2220
Size: 682 484 1054
Fragments arranged by size:
1054 682 484
SanDI GG'GwC_CC
Cuts at: 0 762 952 1181 2220
Size: 762 190 229 1039
Fragments arranged by size:
1039 762 229 190
SbfI CC_TGCA'GG
Cuts at: 0 1802 1952 2220
Size: 1802 150 268
Fragments arranged by size:
1802 268 150
SexAI A'CCwGG_T
Cuts at: 0 233 2220
Size: 233 1987
SfcI C'TryA_G
Cuts at: 0 250 529 942 1300 1773 1798 1868 1895
Size: 250 279 413 358 473 25 70 27
Cuts at: 1895 1924 1948 2031 2220
Size: 29 24 83 189
Fragments arranged by size:
473 413 358 279 250 189 83 70
29 27 25 24
SmaI CCC'GGG
Cuts at: 0 1679 2081 2220
Size: 1679 402 139
Fragments arranged by size:
1679 402 139
StuI AGG'CCT
Cuts at: 0 278 295 2205 2220
Size: 278 17 1910 15
Fragments arranged by size:
1910 278 17 15
StyI C'CwwG_G
Cuts at: 0 599 1571 1901 2220
Size: 599 972 330 319
Fragments arranged by size:
972 599 330 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1104 1510 2220
Size: 1104 406 710
Fragments arranged by size:
1104 710 406
TatI w'GTAC_w
Cuts at: 0 644 2220
Size: 644 1576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 467 499 1191 1223 2220
Size: 196 32 239 32 692 32 997
Fragments arranged by size:
997 692 239 196 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1816 2220
Size: 1816 404
XmaI C'CCGG_G
Cuts at: 0 1677 2079 2220
Size: 1677 402 141
Fragments arranged by size:
1677 402 141
XmnI GAAnn'nnTTC
Cuts at: 0 695 2220
Size: 695 1525
Enzymes that do cut:
AccI Acc65I AcuI AfeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII KpnI MmeI MslI MspA1I NcoI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SbfI
SexAI SfcI SmaI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AleI AloI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI
BfrBI BglI BmgBI BmtI BpuEI BsaBI BsmI BsmBI
BspHI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI