(Circular) (Six-base) MAPSORT of: pFN21AB6378.seq Check: 9481 from: 1 to: 5602 pFN21AB6378 5602 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2850 2945 1937 Size: 913 95 4594 Fragments arranged by size: 4594 913 95 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 5049 5317 1632 Size: 2033 1384 268 1917 Fragments arranged by size: 2033 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2636 1184 Size: 1452 4150 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2754 2786 2806 2838 2754 Size: 32 20 32 5518 Fragments arranged by size: 5518 32 32 20 AlwNI CAG_nnn'CTG Cuts at: 3345 4917 3345 Size: 1572 4030 ApaI G_GGCC'C Cuts at: 2751 2751 Size: 5602 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2927 1870 Size: 73 984 4545 Fragments arranged by size: 4545 984 73 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 4378 618 Size: 325 217 627 1136 1455 1842 Fragments arranged by size: 1842 1455 1136 627 325 217 BanII G_rGCy'C Cuts at: 1391 2093 2174 2414 2751 2837 2921 1391 Size: 702 81 240 337 86 84 4072 Fragments arranged by size: 4072 702 337 240 86 84 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3793 3827 1903 Size: 34 50 34 1772 34 3678 Fragments arranged by size: 3678 1772 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 2058 2392 1548 Size: 510 334 4758 Fragments arranged by size: 4758 510 334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2457 2633 4213 Size: 107 122 71 631 605 785 176 1580 Cuts at: 4213 136 Size: 1525 Fragments arranged by size: 1580 1525 785 631 605 176 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 2831 3019 2831 Size: 188 5414 Bme1580I G_kGCm'C Cuts at: 1790 2303 2751 3663 4819 1790 Size: 513 448 912 1156 2573 Fragments arranged by size: 2573 1156 912 513 448 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3333 4291 442 Size: 416 53 83 399 24 1916 958 1753 Fragments arranged by size: 1916 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2722 2722 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2442 4262 1239 Size: 855 348 1820 2579 Fragments arranged by size: 2579 1820 855 348 Bpu10I CC'TnA_GC Cuts at: 2092 2092 Size: 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2502 4265 882 Size: 617 1003 1763 2219 Fragments arranged by size: 2219 1763 1003 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 3791 4426 275 Size: 53 83 186 936 1876 382 635 1451 Fragments arranged by size: 1876 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2156 2165 2186 1178 Size: 30 165 30 732 21 9 21 4594 Fragments arranged by size: 4594 732 165 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2478 2481 2909 1186 Size: 1292 3 428 3879 Fragments arranged by size: 3879 1292 428 3 BseYI C'CCAG_C Cuts at: 2296 2426 2592 4805 5301 2296 Size: 130 166 2213 496 2597 Fragments arranged by size: 2597 2213 496 166 130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2093 2837 3663 3748 4819 2093 Size: 744 826 85 1071 2876 Fragments arranged by size: 2876 1071 826 744 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2174 2303 2414 2751 2837 2921 Size: 399 303 81 129 111 337 86 84 Cuts at: 2921 3663 3748 4819 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 337 303 129 111 86 85 84 81 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2628 2957 844 Size: 1784 329 3489 Fragments arranged by size: 3489 1784 329 BsrBI CCG'CTC Cuts at: 1394 1823 2011 3489 1394 Size: 429 188 1478 3507 Fragments arranged by size: 3507 1478 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 2685 4097 4271 65 Size: 1183 1437 1412 174 1396 Fragments arranged by size: 1437 1412 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2932 3299 3684 3701 5153 5239 5251 5597 Size: 960 367 385 17 1452 86 12 346 Cuts at: 5597 1972 Size: 1977 Fragments arranged by size: 1977 1452 960 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 2564 2577 513 Size: 552 1380 119 13 3538 Fragments arranged by size: 3538 1380 552 119 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 2371 3118 3912 3932 2371 Size: 747 794 20 4041 Fragments arranged by size: 4041 794 747 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567 Size: 54 1425 50 275 18 1219 889 1629 Cuts at: 5567 8 Size: 43 Fragments arranged by size: 1629 1425 1219 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 3532 1353 Size: 680 1499 3423 Fragments arranged by size: 3423 1499 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2535 4189 4563 4709 387 Size: 1064 138 946 1654 374 146 1280 Fragments arranged by size: 1654 1280 1064 946 374 146 138 EcoICRI GAG'CTC Cuts at: 2091 2835 2091 Size: 744 4858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2442 3665 4262 5049 5317 1239 Size: 393 462 348 1223 597 787 268 1524 Fragments arranged by size: 1524 1223 787 597 462 393 348 268 EcoNI CCTnn'n_nnAGG Cuts at: 2668 2668 Size: 5602 EcoO109I rG'GnC_Cy Cuts at: 2747 2748 2747 Size: 1 5601 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2663 2663 Size: 5602 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 4749 1678 Size: 3071 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2225 2257 2486 Size: 32 135 32 563 32 58 32 229 Cuts at: 2486 2518 4243 4275 4317 4349 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 563 229 135 58 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2587 2946 3192 669 Size: 988 281 649 359 246 3079 Fragments arranged by size: 3079 988 649 359 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2303 2817 4716 4900 1305 Size: 816 182 514 1899 184 2007 Fragments arranged by size: 2007 1899 816 514 184 182 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3780 Fragments arranged by size: 3780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 3560 3919 4078 518 Size: 648 1066 1328 359 159 2042 Fragments arranged by size: 2042 1328 1066 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2296 2306 2447 3018 3695 4843 5088 1606 Size: 690 10 141 571 677 1148 245 2120 Fragments arranged by size: 2120 1148 690 677 571 245 141 10 NcoI C'CATG_G Cuts at: 513 1065 2564 2577 513 Size: 552 1499 13 3538 Fragments arranged by size: 3538 1499 552 13 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2960 4505 1132 Size: 97 1731 1545 2229 Fragments arranged by size: 2229 1731 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2747 2747 Size: 5602 PstI C_TGCA'G Cuts at: 830 2954 830 Size: 2124 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2296 2306 2296 Size: 10 5592 SacI G_AGCT'C Cuts at: 2093 2837 2093 Size: 744 4858 SacII CC_GC'GG Cuts at: 2448 2448 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2847 2950 4085 4766 4957 826 Size: 219 1802 103 1135 681 191 1471 Fragments arranged by size: 1802 1471 1135 681 219 191 103 SfiI GGCCn_nnn'nGGCC Cuts at: 2633 2633 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 2705 2800 1517 Size: 1188 95 4319 Fragments arranged by size: 4319 1188 95 StyI C'CwwG_G Cuts at: 513 1065 2126 2564 2577 2877 3041 513 Size: 552 1061 438 13 300 164 3074 Fragments arranged by size: 3074 1061 552 438 300 164 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2411 2740 2411 Size: 329 5273 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI