Restriction map for
pFN21AB8878
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeIII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 106 GGTACC G^GTACC
AfaI 107 GTAC GT^AC
Asp718I 106 GGTACC G^GTACC
AspI 33 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 338 CCCGGG C^[CT]CG[GA]G
BanI 106 GGTACC G^G[CT][GA]CC
BfaI 400 CTAG C^TAG
BpmI 245 CTCCAG CTGGAG(16/14)
BsgI 496 CTGCAC GTGCAG(16/14)
BsmFI 31 GGGAC GGGAC(10/14)
BsoBI 338 CCCGGG C^[CT]CG[GA]G
BspMI 71 ACCTGC ACCTGC(4/8)
BsrDI 14 CATTGC GCAATG(2/0)
BsrI 254 CCAGT ACTGG(1/-1)
Cac8I 399 GCTAGC GC[ACGT]^[ACGT]GC
EcoT14I 294 CCAAGG C^C[AT][AT]GG
HaeII 437 AGCGCC [GA]GCGC^[CT]
HapII 339 CCGG C^CGG
HpaII 339 CCGG C^CGG
KpnI 106 GGTACC GGTAC^C
MaeI 400 CTAG C^TAG
MaeIII 217 GTCAC ^GT[ACGT]AC
MspI 339 CCGG C^CGG
NheI 399 GCTAGC G^CTAGC
NlaIII 357 CATG CATG^
PflFI 33 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 107 GTAC GT^AC
SmaI 338 CCCGGG CCC^GGG
SmlI 421 CTCAAG C^T[CT][GA]AG
StyI 294 CCAAGG C^C[AT][AT]GG
TaqI 434 TCGA T^CGA
Tsp45I 217 GTCAC ^GT[GC]AC
TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 33 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 434 TCGA T^CGA
XcmI 41 CCAGCTGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 338 CCCGGG C^CCGGG
XmaI 338 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AspHI 261 GAGCTC G[AT]GC[AT]^C
470 GAGCTC
AvaII 69 GGACC G^G[AT]CC
81 GGTCC
BbsI 289 GAAGAC GAAGAC(2/6)
87 GTCTTC
BbvI 4 GCAGC GCAGC(8/12)
351 GCAGC
BcnI 338 CCCGG CC^[GC]GG
339 CCGGG
BpuAI 289 GAAGAC GAAGAC(2/6)
87 GTCTTC
BsiHKAI 261 GAGCTC G[AT]GC[AT]^C
470 GAGCTC
CfoI 354 GCGC GCG^C
438 GCGC
Cfr13I 69 GGACC G^G[ACGT]CC
81 GGTCC
DraII 68 AGGACCT [GA]G^G[ACGT]CC[CT]
80 AGGTCCT
EcoNI 72 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
267 CCTGGCTCAGG
EcoO109I 68 AGGACCT [GA]G^G[ACGT]CC[CT]
80 AGGTCCT
Fnu4HI 4 GCAGC GC^[ACGT]GC
351 GCAGC
HhaI 354 GCGC GCG^C
438 GCGC
HinP1I 354 GCGC G^CGC
438 GCGC
ItaI 4 GCAGC GC^[ACGT]GC
351 GCAGC
MspA1I 5 CAGCTG C[AC]G^C[GT]G
42 CAGCTG
MwoI 172 GCTTCTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
394 GCACAGCTAGC
NciI 338 CCCGG CC^[GC]GG
339 CCGGG
NlaIV 106 GGTACC GG[ACGT]^[ACGT]CC
198 GGAGCC
PleI 462 GACTC GAGTC(4/5)
10 GAGTC
PpuMI 68 AGGACCT [GA]G^G[AT]CC[CT]
80 AGGTCCT
PvuII 5 CAGCTG CAG^CTG
42 CAGCTG
SacI 261 GAGCTC GAGCT^C
470 GAGCTC
Sau96I 69 GGACC G^G[ACGT]CC
81 GGTCC
TseI 4 GCAGC G^C[AT]GC
351 GCAGC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BanII BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstNI
DdeI EarI EcoRII HinfI HphI
Ksp632I MboII MnlI MvaI ScrFI
TfiI