Restriction map for pFN21AB8878
 (Circular) (Six-base) MAPSORT of: pFN21AB8878.seq  Check: 1928  from: 1  to: 5212



pFN21AB8878  5212 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:36  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3022     278 
   Size:         53      83     186     936    1486    2468

  Fragments arranged by size: 

                2468    1486     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2555    1937 
   Size:        618    4594
 
Acc65I G'GTAC_C

Cuts at:   2111    2533    2111 
   Size:        422    4790
 
AclI AA'CG_TT

Cuts at:   1563    3339    3712    1563 
   Size:       1776     373    3063

  Fragments arranged by size: 

                3063    1776     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2079    2430    3275    4659    4927    1632 
   Size:        447     351     845    1384     268    1917

  Fragments arranged by size: 

                1917    1384     845     447     351     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5015
 
AflIII A'CryG_T

Cuts at:   1203    4111    5088    1203 
   Size:       2908     977    1327

  Fragments arranged by size: 

                2908    1327     977
 
AgeI A'CCGG_T

Cuts at:   4043    4043 
   Size:       5212
 
AhdI GACnn_n'nnGTC

Cuts at:   3941    3941 
   Size:       5212
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5212
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5212
 
AlwNI CAG_nnn'CTG

Cuts at:   2955    4527    2955 
   Size:       1572    3640
 
ApaLI G'TGCA_C

Cuts at:   3269    4425    3269 
   Size:       1156    4056
 
ApoI r'AATT_y

Cuts at:   1493    2521    2747    2899    5008    1493 
   Size:       1028     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1028     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3766     160 
   Size:        872    2734    1606

  Fragments arranged by size: 

                2734    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5212
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2343    2537    1870 
   Size:         73     400     194    4545

  Fragments arranged by size: 

                4545     400     194      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2103    2136    2103 
   Size:         33    5179
 
BamHI G'GATC_C

Cuts at:   2542    2909    2542 
   Size:        367    4845
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2111    2533    3988     618 
   Size:        325     217     627     324     422    1455    1842

  Fragments arranged by size: 

                1842    1455     627     422     325     324     217
 
BanII G_rGCy'C

Cuts at:   1391    2243    2270    2479    2531    1391 
   Size:        852      27     209      52    4072

  Fragments arranged by size: 

                4072     852     209      52      27
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2085    2301     928 
   Size:        971     186     216    3839

  Fragments arranged by size: 

                3839     971     216     186
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3403    3437    1903 
   Size:         34    1466      34    3678

  Fragments arranged by size: 

                3678    1466      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3104    4314    3104 
   Size:       1210    4002
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5212
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3823     136 
   Size:        107     122      71     631     605    2151    1525

  Fragments arranged by size: 

                2151    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5207    5207 
   Size:       5212
 
BlpI GC'TnA_GC

Cuts at:   2629    2629 
   Size:       5212
 
Bme1580I G_kGCm'C

Cuts at:   1790    3273    4429    1790 
   Size:       1483    1156    2573

  Fragments arranged by size: 

                2573    1483    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5212
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2943    3901     442 
   Size:        416      53      83     399      24    1526     958    1753

  Fragments arranged by size: 

                1753    1526     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2408    1056 
   Size:       1352    3860
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2235    3872    1239 
   Size:        996    1637    2579

  Fragments arranged by size: 

                2579    1637     996
 
Bpu10I CC'TnA_GC

Cuts at:   2277    2277 
   Size:       5212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2411    3337    4202    4500    2411 
   Size:        926     865     298    3123

  Fragments arranged by size: 

                3123     926     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3875     882 
   Size:        617    2376    2219

  Fragments arranged by size: 

                2376    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3003     493 
   Size:        711    1799    2702

  Fragments arranged by size: 

                2702    1799     711
 
BsaBI GATnn'nnATC

Cuts at:   5206    5206 
   Size:       5212
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3019    3401    4036     275 
   Size:         53      83     186     936    1486     382     635    1451

  Fragments arranged by size: 

                1486    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3645    4043    4317    4464    4875    3645 
   Size:        398     274     147     411    3982

  Fragments arranged by size: 

                3982     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4987

  Fragments arranged by size: 

                4987     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2213    2216    2303    1186 
   Size:       1027       3      87    4095

  Fragments arranged by size: 

                4095    1027      87       3
 
BseYI C'CCAG_C

Cuts at:   2049    2338    4415    4911    2049 
   Size:        289    2077     496    2350

  Fragments arranged by size: 

                2350    2077     496     289
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2486    1163 
   Size:        573     750    3889

  Fragments arranged by size: 

                3889     750     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2662    3423    4451    1540 
   Size:        275     186     661     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     661     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2270    2479    3273    3358    4429    2270 
   Size:        209     794      85    1071    3053

  Fragments arranged by size: 

                3053    1071     794     209      85
 
BsmI GAATG_Cn'

Cuts at:   2723    2816    2723 
   Size:         93    5119
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2243    2270    2479    2531    3273    3358    4429 
   Size:        399     453      27     209      52     742      85    1071

Cuts at:   4429    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     453     399     209      85      52
                  27
 
BspEI T'CCGG_A

Cuts at:   4875    4875 
   Size:       5212
 
BspHI T'CATG_A

Cuts at:   3101    4831    3101 
   Size:       1730    3482
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2085    2567     844 
   Size:       1241     482    3489

  Fragments arranged by size: 

                3489    1241     482
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3099    1394 
   Size:        429    1276    3507

  Fragments arranged by size: 

                3507    1276     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2018    3707    3881      65 
   Size:       1183     770    1689     174    1396

  Fragments arranged by size: 

                1689    1396    1183     770     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3856    4043    1700 
   Size:        110    2046     187    2869

  Fragments arranged by size: 

                2869    2046     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5212
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5212
 
BssSI C'ACGA_G

Cuts at:   3272    4284    3272 
   Size:       1012    4200
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5212
 
BstBI TT'CG_AA

Cuts at:   4016    4016 
   Size:       5212
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5212
 
BstYI r'GATC_y

Cuts at:   1972    2542    2909    3294    3311    4763    4849    4861    5207 
   Size:        570     367     385      17    1452      86      12     346

Cuts at:   5207    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     570     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3721

  Fragments arranged by size: 

                3721     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4473

  Fragments arranged by size: 

                4473     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2728    3522    3542    2728 
   Size:        794      20    4398

  Fragments arranged by size: 

                4398     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5212
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5177
 
DraI TTT'AAA

Cuts at:   2516    2516 
   Size:       5212
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4219     809 
   Size:        507    2903    1802

  Fragments arranged by size: 

                2903    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2659    3548    5177 
   Size:         54    1425      50     275      18     829     889    1629

Cuts at:   5177       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     829     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2659    1537 
   Size:        275     847    4090

  Fragments arranged by size: 

                4090     847     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2133    2280    2406    3142    1353 
   Size:        780     147     126     736    3423

  Fragments arranged by size: 

                3423     780     736     147     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3799    4173    4319     387 
   Size:       1064     138    2210     374     146    1280

  Fragments arranged by size: 

                2210    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2268    2477    2268 
   Size:        209    5003
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2079    2235    2430    3275    3872    4659    4927 
   Size:        393     447     156     195     845     597     787     268

Cuts at:   4927    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     845     787     597     447     393     268     195
                 156
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2081    2276    2081 
   Size:        195    5017
 
EcoO109I rG'GnC_Cy

Cuts at:   2074    2086    2074 
   Size:         12    5200
 
EcoRI G'AATT_C

Cuts at:   2521    2899    2521 
   Size:        378    4834
 
FspI TGC'GCA

Cuts at:   3718    3718 
   Size:       5212
 
HaeII r_GCGC'y

Cuts at:   1678    2446    4359    1678 
   Size:        768    1913    2531

  Fragments arranged by size: 

                2531    1913     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2075    2107    2132    2164    3853 
   Size:         32     135      32     503      32      25      32    1689

Cuts at:   3853    3885    3927    3959    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1689     503     135      42      32      32      32
                  32      32      32      25
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2556    2802     669 
   Size:        988     281     618     246    3079

  Fragments arranged by size: 

                3079     988     618     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5212
 
HpaI GTT'AAC

Cuts at:   2802    2802 
   Size:       5212
 
KpnI G_GTAC'C

Cuts at:   2115    2537    2115 
   Size:        422    4790
 
MfeI C'AATT_G

Cuts at:   2811    2811 
   Size:       5212
 
MluI A'CGCG_T

Cuts at:   5088    5088 
   Size:       5212
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4326    4510    1305 
   Size:       3021     184    2007

  Fragments arranged by size: 

                3021    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3390

  Fragments arranged by size: 

                3390    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3170    3529    3688     518 
   Size:        648    2004     359     159    2042

  Fragments arranged by size: 

                2042    2004     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2012    2049    2628    3305    4453    4698    1606 
   Size:        406      37     579     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     579     406     245      37
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3721

  Fragments arranged by size: 

                3721     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5212
 
NheI G'CTAG_C

Cuts at:   1052    2404    1052 
   Size:       1352    3860
 
NotI GC'GGCC_GC

Cuts at:   2659    2659 
   Size:       5212
 
NspI r_CATG'y

Cuts at:   1132    1229    2570    4115    1132 
   Size:         97    1341    1545    2229

  Fragments arranged by size: 

                2229    1545    1341      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5212
 
PciI A'CATG_T

Cuts at:   4111    4111 
   Size:       5212
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5212
 
PfoI T'CCnGG_A

Cuts at:   2128    2128 
   Size:       5212
 
PmeI GTTT'AAAC

Cuts at:   2516    2516 
   Size:       5212
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3281    3313    4794    4826    3281 
   Size:         32    1481      32    3667

  Fragments arranged by size: 

                3667    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2074    2086    2074 
   Size:         12    5200
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5212
 
PsiI TTA'TAA

Cuts at:   2782    2782 
   Size:       5212
 
PstI C_TGCA'G

Cuts at:    830    2564     830 
   Size:       1734    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5212
 
PvuII CAG'CTG

Cuts at:   2012    2049    2012 
   Size:         37    5175
 
SacI G_AGCT'C

Cuts at:   2270    2479    2270 
   Size:        209    5003
 
SalI G'TCGA_C

Cuts at:   1936    2554    1936 
   Size:        618    4594
 
SbfI CC_TGCA'GG

Cuts at:   2564    2564 
   Size:       5212
 
ScaI AGT'ACT

Cuts at:   3460    5172    3460 
   Size:       1712    3500
 
SfcI C'TryA_G

Cuts at:    826    1045    2560    3695    4376    4567     826 
   Size:        219    1515    1135     681     191    1471

  Fragments arranged by size: 

                1515    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5212
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5212
 
SmaI CCC'GGG

Cuts at:   1872    2345    2539    1872 
   Size:        473     194    4545

  Fragments arranged by size: 

                4545     473     194
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2426    3316    4217    4479     820 
   Size:        197     926     483     890     901     262    1553

  Fragments arranged by size: 

                1553     926     901     890     483     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3003     493 
   Size:       2510    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5212
 
SphI G_CATG'C

Cuts at:   1132    2570    1132 
   Size:       1438    3774
 
SrfI GCCC'GGGC

Cuts at:   2345    2345 
   Size:       5212
 
SspI AAT'ATT

Cuts at:      5      52    3136       5 
   Size:         47    3084    2081

  Fragments arranged by size: 

                3084    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5212
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2299    2651     513 
   Size:        552     939     295     352    3074

  Fragments arranged by size: 

                3074     939     552     352     295
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2502    3239    3256    3409    3594    4039     325 
   Size:       1255     922     737      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     922     737     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3458    5170      96 
   Size:        274      80      33      51    1443    1481    1712     138

  Fragments arranged by size: 

                1712    1481    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2311    2343    5172    5204    2311 
   Size:         32    2829      32    2319

  Fragments arranged by size: 

                2829    2319      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2041    1316 
   Size:        725    4487
 
XbaI T'CTAG_A

Cuts at:   2548    2548 
   Size:       5212
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2053    2053 
   Size:       5212
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5212
 
XmaI C'CCGG_G

Cuts at:   1870    2343    2537    1870 
   Size:        473     194    4545

  Fragments arranged by size: 

                4545     473     194
 
XmnI GAAnn'nnTTC

Cuts at:   3341    3341 
   Size:       5212
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3020     276 
   Size:         53      83     186     936    1486    2468

  Fragments arranged by size: 

                2468    1486     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SacI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SwaI
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