Restriction map for
pFN21AA1512
(Linear) (Six-base) MAPSORT of: KIAA1512.seq Check: 5260 from: 1 to: 5949
KIAA1512.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1380 3753 5568 5949
Size: 1380 2373 1815 381
Fragments arranged by size:
2373 1815 1380 381
AccI GT'mk_AC
Cuts at: 0 989 5949
Size: 989 4960
Acc65I G'GTAC_C
Cuts at: 0 2309 5949
Size: 2309 3640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 3343 5473 5949
Size: 1140 2203 2130 476
Fragments arranged by size:
2203 2130 1140 476
AfeI AGC'GCT
Cuts at: 0 445 2857 5949
Size: 445 2412 3092
Fragments arranged by size:
3092 2412 445
AflII C'TTAA_G
Cuts at: 0 5656 5949
Size: 5656 293
AflIII A'CryG_T
Cuts at: 0 1610 5949
Size: 1610 4339
AhdI GACnn_n'nnGTC
Cuts at: 0 636 5949
Size: 636 5313
AleI CACnn'nnGTG
Cuts at: 0 44 113 2431 4163 5949
Size: 44 69 2318 1732 1786
Fragments arranged by size:
2318 1786 1732 69 44
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1735 1767 2563 2595 5949
Size: 1735 32 796 32 3354
Fragments arranged by size:
3354 1735 796 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1473 2487 2556 2763 3165 3372 3795 4152
Size: 1473 1014 69 207 402 207 423 357
Cuts at: 4152 4224 4462 4465 4722 5133 5166 5589 5745
Size: 72 238 3 257 411 33 423 156
Cuts at: 5745 5949
Size: 204
Fragments arranged by size:
1473 1014 423 423 411 402 357 257
238 207 207 204 156 72 69 33
3
ApaI G_GGCC'C
Cuts at: 0 3453 3537 4797 4968 5935 5949
Size: 3453 84 1260 171 967 14
Fragments arranged by size:
3453 1260 967 171 84 14
ApaLI G'TGCA_C
Cuts at: 0 196 2985 5474 5949
Size: 196 2789 2489 475
Fragments arranged by size:
2789 2489 475 196
AvaI C'yCGr_G
Cuts at: 0 1340 3485 3530 5918 5949
Size: 1340 2145 45 2388 31
Fragments arranged by size:
2388 2145 1340 45 31
AvrII C'CTAG_G
Cuts at: 0 1149 5949
Size: 1149 4800
BamHI G'GATC_C
Cuts at: 0 1932 2249 4826 5949
Size: 1932 317 2577 1123
Fragments arranged by size:
2577 1932 1123 317
BanI G'GyrC_C
Cuts at: 0 46 234 775 1195 1893 2309 4094 4579
Size: 46 188 541 420 698 416 1785 485
Cuts at: 4579 5949
Size: 1370
Fragments arranged by size:
1785 1370 698 541 485 420 416 188
46
BanII G_rGCy'C
Cuts at: 0 1121 1413 2017 2033 2389 2474 2996 3453
Size: 1121 292 604 16 356 85 522 457
Cuts at: 3453 3537 4589 4797 4841 4968 5147 5525 5696
Size: 84 1052 208 44 127 179 378 171
Cuts at: 5696 5935 5949
Size: 239 14
Fragments arranged by size:
1121 1052 604 522 457 378 356 292
239 208 179 171 127 85 84 44
16 14
BbeI G_GCGC'C
Cuts at: 0 1199 4098 5949
Size: 1199 2899 1851
Fragments arranged by size:
2899 1851 1199
BbsI GAAGACnn'nnnn_
Cuts at: 0 1653 3437 5503 5949
Size: 1653 1784 2066 446
Fragments arranged by size:
2066 1784 1653 446
BbvCI CC'TCA_GC
Cuts at: 0 2681 4199 4455 5399 5949
Size: 2681 1518 256 944 550
Fragments arranged by size:
2681 1518 944 550 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3823 3857 4261 4295 5949
Size: 3823 34 404 34 1654
Fragments arranged by size:
3823 1654 404 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2326 5949
Size: 2326 3623
BclI T'GATC_A
Cuts at: 0 647 2870 4037 5949
Size: 647 2223 1167 1912
Fragments arranged by size:
2223 1912 1167 647
BfrBI ATG'CAT
Cuts at: 0 1748 5949
Size: 1748 4201
BglI GCCn_nnn'nGGC
Cuts at: 0 817 2704 3712 4147 4249 5059 5416 5440
Size: 817 1887 1008 435 102 810 357 24
Cuts at: 5440 5949
Size: 509
Fragments arranged by size:
1887 1008 817 810 509 435 357 102
24
BglII A'GATC_T
Cuts at: 0 1532 5949
Size: 1532 4417
BlpI GC'TnA_GC
Cuts at: 0 2825 3406 3415 3583 3913 4048 4211 5141
Size: 2825 581 9 168 330 135 163 930
Cuts at: 5141 5356 5618 5949
Size: 215 262 331
Fragments arranged by size:
2825 930 581 331 330 262 215 168
163 135 9
Bme1580I G_kGCm'C
Cuts at: 0 200 778 2989 3453 3537 4131 4503 4582
Size: 200 578 2211 464 84 594 372 79
Cuts at: 4582 4797 4968 5478 5913 5935 5949
Size: 215 171 510 435 22 14
Fragments arranged by size:
2211 594 578 510 464 435 372 215
200 171 84 79 22 14
BmgBI CAC'GTC
Cuts at: 0 2778 5949
Size: 2778 3171
BmrI ACTGGGnnnn_n'
Cuts at: 0 107 511 1665 1726 2321 2403 4248 5949
Size: 107 404 1154 61 595 82 1845 1701
Fragments arranged by size:
1845 1701 1154 595 404 107 82 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 403 958 1642 1756 1939 2263 2785
Size: 67 336 555 684 114 183 324 522
Cuts at: 2785 2911 2944 2995 3037 3247 3280 3331 3394
Size: 126 33 51 42 210 33 51 63
Cuts at: 3394 3532 3595 3616 3700 3817 4324 4456 4462
Size: 138 63 21 84 117 507 132 6
Cuts at: 4462 4525 4567 4651 4744 4798 4819 4825 4855
Size: 63 42 84 93 54 21 6 30
Cuts at: 4855 5041 5188 5221 5242 5356 5509 5524 5611
Size: 186 147 33 21 114 153 15 87
Cuts at: 5611 5632 5949
Size: 21 317
Fragments arranged by size:
684 555 522 507 336 324 317 210
186 183 153 147 138 132 126 117
114 114 93 87 84 84 67 63
63 63 54 51 51 42 42 33
33 33 30 21 21 21 21 15
6 6
Bpu10I CC'TnA_GC
Cuts at: 0 2681 4199 4455 5399 5949
Size: 2681 1518 256 944 550
Fragments arranged by size:
2681 1518 944 550 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 2563 2980 3341 3370 4624 4972 5080
Size: 802 1761 417 361 29 1254 348 108
Cuts at: 5080 5557 5949
Size: 477 392
Fragments arranged by size:
1761 1254 802 477 417 392 361 348
108 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 1939 4804 5949
Size: 301 1638 2865 1145
Fragments arranged by size:
2865 1638 1145 301
BsaAI yAC'GTr
Cuts at: 0 258 1365 5949
Size: 258 1107 4584
Fragments arranged by size:
4584 1107 258
BsaBI GATnn'nnATC
Cuts at: 0 2254 5949
Size: 2254 3695
BsaHI Gr'CG_yC
Cuts at: 0 251 1082 1196 1769 2459 4095 5924 5949
Size: 251 831 114 573 690 1636 1829 25
Fragments arranged by size:
1829 1636 831 690 573 251 114 25
BsaWI w'CCGG_w
Cuts at: 0 652 2252 4865 5949
Size: 652 1600 2613 1084
Fragments arranged by size:
2613 1600 1084 652
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2968 2998 4015 4045 5949
Size: 2968 30 1017 30 1904
Fragments arranged by size:
2968 1904 1017 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 91 846 1129 1258 1309 1645 1753
Size: 88 3 755 283 129 51 336 108
Cuts at: 1753 2698 2701 2782 2866 2947 2950 3199 3391
Size: 945 3 81 84 81 3 249 192
Cuts at: 3391 3508 3520 3592 3613 3697 3952 4036 4120
Size: 117 12 72 21 84 255 84 84
Cuts at: 4120 4204 4207 4275 4312 4321 4363 4540 4543
Size: 84 3 68 37 9 42 177 3
Cuts at: 4543 4627 4681 4795 4822 4903 5116 5137 5218
Size: 84 54 114 27 81 213 21 81
Cuts at: 5218 5386 5416 5461 5554 5725 5809 5881 5949
Size: 168 30 45 93 171 84 72 68
Fragments arranged by size:
945 755 336 283 255 249 213 192
177 171 168 129 117 114 108 93
88 84 84 84 84 84 84 84
81 81 81 81 72 72 68 68
54 51 45 42 37 30 27 21
21 12 9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1522 2090 2382 2446 2559 3657 3753 5031
Size: 1522 568 292 64 113 1098 96 1278
Cuts at: 5031 5351 5843 5949
Size: 320 492 106
Fragments arranged by size:
1522 1278 1098 568 492 320 292 113
106 96 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1189 2080 2905 3575 3899 4165 4757
Size: 412 777 891 825 670 324 266 592
Cuts at: 4757 5363 5602 5949
Size: 606 239 347
Fragments arranged by size:
891 825 777 670 606 592 412 347
324 266 239
BsiEI CG_ry'CG
Cuts at: 0 2744 5949
Size: 2744 3205
BsiHKAI G_wGCw'C
Cuts at: 0 200 1121 2474 2989 2996 4381 4589 4841
Size: 200 921 1353 515 7 1385 208 252
Cuts at: 4841 5147 5478 5525 5696 5949
Size: 306 331 47 171 253
Fragments arranged by size:
1385 1353 921 515 331 306 253 252
208 200 171 47 7
BsmI GAATG_Cn'
Cuts at: 0 881 1750 3256 5295 5949
Size: 881 869 1506 2039 654
Fragments arranged by size:
2039 1506 881 869 654
BsmBI CGTCTCn'nnnn_
Cuts at: 0 722 2764 4627 5949
Size: 722 2042 1863 1322
Fragments arranged by size:
2042 1863 1322 722
Bsp1286I G_dGCh'C
Cuts at: 0 200 778 1121 1413 2017 2033 2389 2474
Size: 200 578 343 292 604 16 356 85
Cuts at: 2474 2989 2996 3453 3537 4131 4381 4503 4582
Size: 515 7 457 84 594 250 122 79
Cuts at: 4582 4589 4797 4841 4968 5147 5478 5525 5696
Size: 7 208 44 127 179 331 47 171
Cuts at: 5696 5913 5935 5949
Size: 217 22 14
Fragments arranged by size:
604 594 578 515 457 356 343 331
292 250 217 208 200 179 171 127
122 85 84 79 47 44 22 16
14 7 7
BspEI T'CCGG_A
Cuts at: 0 2252 5949
Size: 2252 3697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 1380 1691 2138 3182 3753 4667 5568
Size: 443 937 311 447 1044 571 914 901
Cuts at: 5568 5949
Size: 381
Fragments arranged by size:
1044 937 914 901 571 447 443 381
311
BsrBI CCG'CTC
Cuts at: 0 563 2919 3294 3522 3591 4446 5360 5871
Size: 563 2356 375 228 69 855 914 511
Cuts at: 5871 5949
Size: 78
Fragments arranged by size:
2356 914 855 563 511 375 228 78
69
BsrDI GCAATG_nn'
Cuts at: 0 2238 4977 5949
Size: 2238 2739 972
Fragments arranged by size:
2739 2238 972
BsrFI r'CCGG_y
Cuts at: 0 667 1433 2963 5240 5949
Size: 667 766 1530 2277 709
Fragments arranged by size:
2277 1530 766 709 667
BssHII G'CGCG_C
Cuts at: 0 3200 5949
Size: 3200 2749
BssSI C'ACGA_G
Cuts at: 0 3700 4186 4609 5149 5848 5949
Size: 3700 486 423 540 699 101
Fragments arranged by size:
3700 699 540 486 423 101
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 1032 3783 4152 4224 4465 5514 5949
Size: 435 597 2751 369 72 241 1049 435
Fragments arranged by size:
2751 1049 597 435 435 369 241 72
BstBI TT'CG_AA
Cuts at: 0 1553 5949
Size: 1553 4396
BstEII G'GTnAC_C
Cuts at: 0 3169 3316 5005 5949
Size: 3169 147 1689 944
Fragments arranged by size:
3169 1689 944 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 967 5949
Size: 967 4982
BstYI r'GATC_y
Cuts at: 0 998 1532 1932 2249 2255 4763 4826 5949
Size: 998 534 400 317 6 2508 63 1123
Fragments arranged by size:
2508 1123 998 534 400 317 63 6
Bsu36I CC'TnA_GG
Cuts at: 0 841 4903 5949
Size: 841 4062 1046
Fragments arranged by size:
4062 1046 841
BtgI C'CryG_G
Cuts at: 0 42 208 302 812 914 1091 1139 1949
Size: 42 166 94 510 102 177 48 810
Cuts at: 1949 2523 3617 4053 4569 5562 5949
Size: 574 1094 436 516 993 387
Fragments arranged by size:
1094 993 810 574 516 510 436 387
177 166 102 94 48 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1107 1503 5949
Size: 1107 396 4446
Fragments arranged by size:
4446 1107 396
BtsI GCAGTG_nn'
Cuts at: 0 119 1314 3718 4327 5949
Size: 119 1195 2404 609 1622
Fragments arranged by size:
2404 1622 1195 609 119
DraIII CAC_nnn'GTG
Cuts at: 0 4143 5949
Size: 4143 1806
DrdI GACnn_nn'nnGTC
Cuts at: 0 3336 5949
Size: 3336 2613
EaeI y'GGCC_r
Cuts at: 0 392 1415 2421 2489 2526 2741 2954 3029
Size: 392 1023 1006 68 37 215 213 75
Cuts at: 3029 4067 4605 5432 5441 5825 5949
Size: 1038 538 827 9 384 124
Fragments arranged by size:
1038 1023 1006 827 538 392 384 215
213 124 75 68 37 9
EagI C'GGCC_G
Cuts at: 0 2741 5949
Size: 2741 3208
EarI CTCTTCn'nnn_
Cuts at: 0 487 2462 3080 3497 3802 4079 4594 4702
Size: 487 1975 618 417 305 277 515 108
Cuts at: 4702 4750 5949
Size: 48 1199
Fragments arranged by size:
1975 1199 618 515 487 417 305 277
108 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 610 1267 3505 3667 3751 3763 3973
Size: 157 453 657 2238 162 84 12 210
Cuts at: 3973 5356 5815 5899 5949
Size: 1383 459 84 50
Fragments arranged by size:
2238 1383 657 459 453 210 162 157
84 84 50 12
EcoICRI GAG'CTC
Cuts at: 0 1119 2472 2994 4587 4839 5145 5523 5694
Size: 1119 1353 522 1593 252 306 378 171
Cuts at: 5694 5949
Size: 255
Fragments arranged by size:
1593 1353 1119 522 378 306 255 252
171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 403 958 1140 1642 1756 1939 2263
Size: 67 336 555 182 502 114 183 324
Cuts at: 2263 2785 2911 2944 2995 3037 3247 3280 3331
Size: 522 126 33 51 42 210 33 51
Cuts at: 3331 3343 3394 3532 3595 3616 3700 3817 4324
Size: 12 51 138 63 21 84 117 507
Cuts at: 4324 4456 4462 4525 4567 4651 4744 4798 4819
Size: 132 6 63 42 84 93 54 21
Cuts at: 4819 4825 4855 5041 5188 5221 5242 5356 5473
Size: 6 30 186 147 33 21 114 117
Cuts at: 5473 5509 5524 5611 5632 5949
Size: 36 15 87 21 317
Fragments arranged by size:
555 522 507 502 336 324 317 210
186 183 182 147 138 132 126 117
117 114 114 93 87 84 84 67
63 63 54 51 51 51 42 42
36 33 33 33 30 21 21 21
21 15 12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 412 1738 2491 3262 4018 4663 5224
Size: 169 243 1326 753 771 756 645 561
Cuts at: 5224 5539 5949
Size: 315 410
Fragments arranged by size:
1326 771 756 753 645 561 410 315
243 169
EcoO109I rG'GnC_Cy
Cuts at: 0 1314 2238 3162 3243 3449 4062 4793 4794
Size: 1314 924 924 81 206 613 731 1
Cuts at: 4794 5652 5742 5932 5949
Size: 858 90 190 17
Fragments arranged by size:
1314 924 924 858 731 613 206 190
90 81 17 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2454 5949
Size: 2454 3495
FspI TGC'GCA
Cuts at: 0 622 3280 3820 5668 5949
Size: 622 2658 540 1848 281
Fragments arranged by size:
2658 1848 622 540 281
HaeII r_GCGC'y
Cuts at: 0 447 561 1199 2487 2556 2734 2757 2859
Size: 447 114 638 1288 69 178 23 102
Cuts at: 2859 3694 4098 4744 4981 5175 5283 5682 5787
Size: 835 404 646 237 194 108 399 105
Cuts at: 5787 5949
Size: 162
Fragments arranged by size:
1288 835 646 638 447 404 399 237
194 178 162 114 108 105 102 69
23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 622 654 841 873 5949
Size: 259 32 331 32 187 32 5076
Fragments arranged by size:
5076 331 259 187 32 32 32
HincII GTy'rAC
Cuts at: 0 681 5949
Size: 681 5268
HpaI GTT'AAC
Cuts at: 0 681 5949
Size: 681 5268
KasI G'GCGC_C
Cuts at: 0 1195 4094 5949
Size: 1195 2899 1855
Fragments arranged by size:
2899 1855 1195
KpnI G_GTAC'C
Cuts at: 0 2313 5949
Size: 2313 3636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 4367 5949
Size: 257 4110 1582
Fragments arranged by size:
4110 1582 257
MscI TGG'CCA
Cuts at: 0 394 1417 2423 2491 3031 5827 5949
Size: 394 1023 1006 68 540 2796 122
Fragments arranged by size:
2796 1023 1006 540 394 122 68
MslI CAynn'nnrTG
Cuts at: 0 44 113 124 394 584 1090 1615 2350
Size: 44 69 11 270 190 506 525 735
Cuts at: 2350 2431 3826 4163 5949
Size: 81 1395 337 1786
Fragments arranged by size:
1786 1395 735 525 506 337 270 190
81 69 44 11
MspA1I CmG'CkG
Cuts at: 0 455 916 1239 1560 2094 2159 2223 2307
Size: 455 461 323 321 534 65 64 84
Cuts at: 2307 2661 2817 2889 3372 3414 3619 3780 3948
Size: 354 156 72 483 42 205 161 168
Cuts at: 3948 3954 4047 4221 4462 4485 4539 4833 5031
Size: 6 93 174 241 23 54 294 198
Cuts at: 5031 5130 5140 5355 5949
Size: 99 10 215 594
Fragments arranged by size:
594 534 483 461 455 354 323 321
294 241 215 205 198 174 168 161
156 99 93 84 72 65 64 54
42 23 10 6
NaeI GCC'GGC
Cuts at: 0 2965 5242 5949
Size: 2965 2277 707
Fragments arranged by size:
2965 2277 707
NarI GG'CG_CC
Cuts at: 0 1196 4095 5949
Size: 1196 2899 1854
Fragments arranged by size:
2899 1854 1196
NcoI C'CATG_G
Cuts at: 0 208 812 1139 5949
Size: 208 604 327 4810
Fragments arranged by size:
4810 604 327 208
NgoMIV G'CCGG_C
Cuts at: 0 2963 5240 5949
Size: 2963 2277 709
Fragments arranged by size:
2963 2277 709
NsiI A_TGCA'T
Cuts at: 0 1750 5949
Size: 1750 4199
NspI r_CATG'y
Cuts at: 0 618 1035 1614 1752 5739 5949
Size: 618 417 579 138 3987 210
Fragments arranged by size:
3987 618 579 417 210 138
PasI CC'CwG_GG
Cuts at: 0 723 2188 3165 5928 5949
Size: 723 1465 977 2763 21
Fragments arranged by size:
2763 1465 977 723 21
PciI A'CATG_T
Cuts at: 0 1610 5949
Size: 1610 4339
PflMI CCAn_nnn'nTGG
Cuts at: 0 756 1140 1383 1473 2487 2591 2763 4080
Size: 756 384 243 90 1014 104 172 1317
Cuts at: 4080 4722 5949
Size: 642 1227
Fragments arranged by size:
1317 1227 1014 756 642 384 243 172
104 90
PfoI T'CCnGG_A
Cuts at: 0 935 1733 2240 2336 2462 4715 5949
Size: 935 798 507 96 126 2253 1234
Fragments arranged by size:
2253 1234 935 798 507 126 96
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 5949
Size: 403 32 5514
Fragments arranged by size:
5514 403 32
PpuMI rG'GwC_Cy
Cuts at: 0 1314 2238 3243 5742 5949
Size: 1314 924 1005 2499 207
Fragments arranged by size:
2499 1314 1005 924 207
PshAI GACnn'nnGTC
Cuts at: 0 1951 2776 5949
Size: 1951 825 3173
Fragments arranged by size:
3173 1951 825
PspOMI G'GGCC_C
Cuts at: 0 3449 3533 4793 4964 5931 5949
Size: 3449 84 1260 171 967 18
Fragments arranged by size:
3449 1260 967 171 84 18
PstI C_TGCA'G
Cuts at: 0 353 1028 1075 1688 2513 2816 2822 3179
Size: 353 675 47 613 825 303 6 357
Cuts at: 3179 3482 3779 3947 4220 4340 4664 4940 5030
Size: 303 297 168 273 120 324 276 90
Cuts at: 5030 5762 5949
Size: 732 187
Fragments arranged by size:
825 732 675 613 357 353 324 303
303 297 276 273 187 168 120 90
47 6
PvuII CAG'CTG
Cuts at: 0 1560 2094 2223 2817 2889 3372 3414 3954
Size: 1560 534 129 594 72 483 42 540
Cuts at: 3954 4047 4462 4833 5031 5140 5355 5949
Size: 93 415 371 198 109 215 594
Fragments arranged by size:
1560 594 594 540 534 483 415 371
215 198 129 109 93 72 42
SacI G_AGCT'C
Cuts at: 0 1121 2474 2996 4589 4841 5147 5525 5696
Size: 1121 1353 522 1593 252 306 378 171
Cuts at: 5696 5949
Size: 253
Fragments arranged by size:
1593 1353 1121 522 378 306 253 252
171
SacII CC_GC'GG
Cuts at: 0 917 3620 5949
Size: 917 2703 2329
Fragments arranged by size:
2703 2329 917
SapI GCTCTTCn'nnn_
Cuts at: 0 2462 4594 4702 5949
Size: 2462 2132 108 1247
Fragments arranged by size:
2462 2132 1247 108
SbfI CC_TGCA'GG
Cuts at: 0 3179 4664 4940 5949
Size: 3179 1485 276 1009
Fragments arranged by size:
3179 1485 1009 276
ScaI AGT'ACT
Cuts at: 0 703 5949
Size: 703 5246
SexAI A'CCwGG_T
Cuts at: 0 4688 5744 5949
Size: 4688 1056 205
Fragments arranged by size:
4688 1056 205
SfcI C'TryA_G
Cuts at: 0 349 1024 1071 1684 2509 2812 2818 3175
Size: 349 675 47 613 825 303 6 357
Cuts at: 3175 3478 3775 3943 3985 4216 4336 4408 4660
Size: 303 297 168 42 231 120 72 252
Cuts at: 4660 4936 5026 5758 5949
Size: 276 90 732 191
Fragments arranged by size:
825 732 675 613 357 349 303 303
297 276 252 231 191 168 120 90
72 47 42 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5059 5416 5440 5949
Size: 5059 357 24 509
Fragments arranged by size:
5059 509 357 24
SfoI GGC'GCC
Cuts at: 0 1197 4096 5949
Size: 1197 2899 1853
Fragments arranged by size:
2899 1853 1197
SgrAI Cr'CCGG_yG
Cuts at: 0 2963 5949
Size: 2963 2986
SmaI CCC'GGG
Cuts at: 0 1342 3532 5920 5949
Size: 1342 2190 2388 29
Fragments arranged by size:
2388 2190 1342 29
SmlI C'TyrA_G
Cuts at: 0 781 2542 2995 3356 3385 4639 4987 5095
Size: 781 1761 453 361 29 1254 348 108
Cuts at: 5095 5536 5656 5949
Size: 441 120 293
Fragments arranged by size:
1761 1254 781 453 441 361 348 293
120 108 29
SphI G_CATG'C
Cuts at: 0 5739 5949
Size: 5739 210
SrfI GCCC'GGGC
Cuts at: 0 3532 5949
Size: 3532 2417
StuI AGG'CCT
Cuts at: 0 418 479 820 1328 1762 4942 5312 5949
Size: 418 61 341 508 434 3180 370 637
Fragments arranged by size:
3180 637 508 434 418 370 341 61
StyI C'CwwG_G
Cuts at: 0 64 208 812 1139 1149 1370 1418 1757
Size: 64 144 604 327 10 221 48 339
Cuts at: 1757 2265 2429 2492 2879 3125 3869 4232 5597
Size: 508 164 63 387 246 744 363 1365
Cuts at: 5597 5949
Size: 352
Fragments arranged by size:
1365 744 604 508 387 363 352 339
327 246 221 164 144 64 63 48
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 1830 2422 2803 3157 4309 5949
Size: 222 1608 592 381 354 1152 1640
Fragments arranged by size:
1640 1608 1152 592 381 354 222
TatI w'GTAC_w
Cuts at: 0 701 1634 5949
Size: 701 933 4315
Fragments arranged by size:
4315 933 701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1706 1738 1805 1837 2586 2618 2968 3000
Size: 1706 32 67 32 749 32 350 32
Cuts at: 3000 4015 4047 5949
Size: 1015 32 1902
Fragments arranged by size:
1902 1706 1015 749 350 67 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1657 2085 4416 5949
Size: 1657 428 2331 1533
Fragments arranged by size:
2331 1657 1533 428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 810 2416 2886 3222 3306 3930 4128
Size: 126 684 1606 470 336 84 624 198
Cuts at: 4128 4245 4320 4740 5820 5949
Size: 117 75 420 1080 129
Fragments arranged by size:
1606 1080 684 624 470 420 336 198
129 126 117 84 75
XmaI C'CCGG_G
Cuts at: 0 1340 3530 5918 5949
Size: 1340 2190 2388 31
Fragments arranged by size:
2388 2190 1340 31
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AgeI AlfI ApoI AscI AseI AsiSI
BaeI BmtI BplI BsiWI BspHI BsrGI BstZ17I ClaI
CspCI DraI EcoRI EcoRV FseI FspAI HindIII MfeI
MluI NdeI NheI NotI NruI PacI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SgfI
SnaBI SpeI SspI SwaI XbaI XhoI XmnI ZraI