(Circular) (Six-base) MAPSORT of: pFN21AB6417.seq Check: 5156 from: 1 to: 5632 pFN21AB6417 5632 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2445 2975 1937 Size: 508 530 4594 Fragments arranged by size: 4594 530 508 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 3695 5079 5347 1632 Size: 998 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 998 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2756 2941 3167 3319 5428 1493 Size: 1263 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 1263 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2312 2957 1870 Size: 73 369 645 4545 Fragments arranged by size: 4545 645 369 73 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 2291 2645 2688 2780 Size: 325 217 627 346 158 354 43 92 Cuts at: 2780 2953 4408 618 Size: 173 1455 1842 Fragments arranged by size: 1842 1455 627 354 346 325 217 173 158 92 43 BanII G_rGCy'C Cuts at: 1391 2676 2951 1391 Size: 1285 275 4072 Fragments arranged by size: 4072 1285 275 BbeI G_GCGC'C Cuts at: 2137 2295 2137 Size: 158 5474 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BbvCI CC'TCA_GC Cuts at: 2022 2022 Size: 5632 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3823 3857 1903 Size: 34 50 34 1802 34 3678 Fragments arranged by size: 3678 1802 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2785 3693 4849 1790 Size: 995 908 1156 2573 Fragments arranged by size: 2573 1156 995 908 BmgBI CAC'GTC Cuts at: 1318 2344 1318 Size: 1026 4606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2013 2255 2372 2760 4292 1239 Size: 774 242 117 388 1532 2579 Fragments arranged by size: 2579 1532 774 388 242 117 Bpu10I CC'TnA_GC Cuts at: 2022 2517 2022 Size: 495 5137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2089 4295 882 Size: 617 590 2206 2219 Fragments arranged by size: 2219 2206 617 590 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 2804 5626 2804 Size: 2822 2810 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 2271 2284 2292 Size: 53 83 186 936 601 137 13 8 Cuts at: 2292 3439 3821 4456 275 Size: 1147 382 635 1451 Fragments arranged by size: 1451 1147 936 635 601 382 186 137 83 53 13 8 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2077 2213 2387 2426 1186 Size: 891 136 174 39 4392 Fragments arranged by size: 4392 891 174 136 39 BseYI C'CCAG_C Cuts at: 2329 2356 2363 2549 2837 4835 5331 2329 Size: 27 7 186 288 1998 496 2630 Fragments arranged by size: 2630 1998 496 288 186 27 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2588 2866 1163 Size: 573 852 278 3929 Fragments arranged by size: 3929 852 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2017 2178 3082 3843 4871 1540 Size: 275 186 16 161 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 161 16 BsiHKAI G_wGCw'C Cuts at: 2676 3693 3778 4849 2676 Size: 1017 85 1071 3459 Fragments arranged by size: 3459 1071 1017 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2676 2785 2951 3693 3778 4849 1391 Size: 399 886 109 166 742 85 1071 2174 Fragments arranged by size: 2174 1071 886 742 399 166 109 85 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2733 2987 844 Size: 1889 254 3489 Fragments arranged by size: 3489 1889 254 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4127 4301 65 Size: 1183 1578 1301 174 1396 Fragments arranged by size: 1578 1396 1301 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2223 2962 3329 3714 3731 5183 5269 5281 Size: 251 739 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 739 385 367 346 251 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2846 2846 Size: 5632 BtgI C'CryG_G Cuts at: 513 1065 2043 2459 513 Size: 552 978 416 3686 Fragments arranged by size: 3686 978 552 416 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2646 487 Size: 683 56 1420 3473 Fragments arranged by size: 3473 1420 683 56 BtsI GCAGTG_nn' Cuts at: 2458 3148 3942 3962 2458 Size: 690 794 20 4128 Fragments arranged by size: 4128 794 690 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2453 2633 4639 809 Size: 507 1137 180 2006 1802 Fragments arranged by size: 2006 1802 1137 507 180 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2436 3079 Size: 54 1425 50 275 18 345 261 643 Cuts at: 3079 3968 5597 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 643 345 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2175 3079 1537 Size: 275 363 904 4090 Fragments arranged by size: 4090 904 363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 4219 4593 4739 387 Size: 1064 138 621 2009 374 146 1280 Fragments arranged by size: 2009 1280 1064 621 374 146 138 EcoICRI GAG'CTC Cuts at: 2674 2674 Size: 5632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2255 2372 2630 2760 3695 4292 Size: 393 381 242 117 258 130 935 597 Cuts at: 4292 5079 5347 1239 Size: 787 268 1524 Fragments arranged by size: 1524 935 787 597 393 381 268 258 242 130 117 EcoNI CCTnn'n_nnAGG Cuts at: 2912 2912 Size: 5632 EcoO109I rG'GnC_Cy Cuts at: 2057 2360 2057 Size: 303 5329 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 5632 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2137 2295 4779 1678 Size: 459 158 2484 2531 Fragments arranged by size: 2531 2484 459 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2587 2619 4273 4305 4347 Size: 32 135 32 1015 32 1654 32 42 Cuts at: 4347 4379 1373 Size: 32 2626 Fragments arranged by size: 2626 1654 1015 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KasI G'GCGC_C Cuts at: 2133 2291 2133 Size: 158 5474 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3810 Fragments arranged by size: 3810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 3590 3949 4108 518 Size: 648 960 1464 359 159 2042 Fragments arranged by size: 2042 1464 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2441 3048 3725 4873 5118 1606 Size: 835 607 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 607 245 NarI GG'CG_CC Cuts at: 2134 2292 2134 Size: 158 5474 NcoI C'CATG_G Cuts at: 513 1065 2043 513 Size: 552 978 4102 Fragments arranged by size: 4102 978 552 NdeI CA'TA_TG Cuts at: 387 2511 387 Size: 2124 3508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2125 2990 4535 1132 Size: 97 896 865 1545 2229 Fragments arranged by size: 2229 1545 896 865 97 PasI CC'CwG_GG Cuts at: 1348 2503 1348 Size: 1155 4477 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2892 1108 Size: 1784 3848 PfoI T'CCnGG_A Cuts at: 2029 2029 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2360 2057 Size: 303 5329 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PspXI vC'TCGA_Gb Cuts at: 2312 2312 Size: 5632 PstI C_TGCA'G Cuts at: 830 2053 2730 2984 830 Size: 1223 677 254 3478 Fragments arranged by size: 3478 1223 677 254 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SacI G_AGCT'C Cuts at: 2676 2676 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SanDI GG'GwC_CC Cuts at: 2360 2360 Size: 5632 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 2216 3880 5592 2216 Size: 1664 1712 2256 Fragments arranged by size: 2256 1712 1664 SfcI C'TryA_G Cuts at: 826 1045 2049 2298 2442 2726 2980 4115 4796 Size: 219 1004 249 144 284 254 1135 681 Cuts at: 4796 4987 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1004 681 284 254 249 219 191 144 SfoI GGC'GCC Cuts at: 2135 2293 2135 Size: 158 5474 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 3736 4637 4899 820 Size: 197 926 369 1424 901 262 1553 Fragments arranged by size: 1553 1424 926 901 369 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 2918 1517 Size: 1401 4231 StyI C'CwwG_G Cuts at: 513 1065 2043 2913 3071 513 Size: 552 978 870 158 3074 Fragments arranged by size: 3074 978 870 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 2811 3878 Size: 274 80 33 51 1443 237 597 1067 Cuts at: 3878 5590 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1067 597 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 2312 1943 Size: 369 5263 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 2831 3761 2831 Size: 930 4702 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI