(Circular) (Six-base) MAPSORT of: pFN21AB7954.seq Check: 8860 from: 1 to: 5890 pFN21AB7954 5890 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3233 1937 Size: 1296 4594 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2583 2709 2718 2973 3038 3152 3953 5337 Size: 951 126 9 255 65 114 801 1384 Cuts at: 5337 5605 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 951 801 268 255 126 114 65 9 AflII C'TTAA_G Cuts at: 820 1017 2276 820 Size: 197 1259 4434 Fragments arranged by size: 4434 1259 197 AflIII A'CryG_T Cuts at: 1203 2307 4789 5766 1203 Size: 1104 2482 977 1327 Fragments arranged by size: 2482 1327 1104 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 2243 2771 3633 5205 2243 Size: 528 862 1572 2928 Fragments arranged by size: 2928 1572 862 528 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 2408 2605 2841 2854 3199 3425 3577 5686 Size: 915 197 236 13 345 226 152 2109 Cuts at: 5686 1493 Size: 1697 Fragments arranged by size: 2109 1697 915 345 236 226 197 152 13 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2619 2652 2619 Size: 33 5857 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2780 3211 4666 618 Size: 325 217 627 993 431 1455 1842 Fragments arranged by size: 1842 1455 993 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 2095 3209 1391 Size: 704 1114 4072 Fragments arranged by size: 4072 1114 704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3158 928 Size: 971 1259 3660 Fragments arranged by size: 3660 1259 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4501 136 Size: 107 122 71 631 605 2829 1525 Fragments arranged by size: 2829 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 3307 3307 Size: 5890 Bme1580I G_kGCm'C Cuts at: 1790 3951 5107 1790 Size: 2161 1156 2573 Fragments arranged by size: 2573 2161 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4579 442 Size: 416 53 83 399 24 2204 958 1753 Fragments arranged by size: 2204 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3066 3066 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2586 2718 4550 1239 Size: 1347 132 1832 2579 Fragments arranged by size: 2579 1832 1347 132 Bpu10I CC'TnA_GC Cuts at: 2692 2692 Size: 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4015 4880 5178 4015 Size: 865 298 4727 Fragments arranged by size: 4727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4553 882 Size: 617 3054 2219 Fragments arranged by size: 3054 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2559 2589 3090 3120 1178 Size: 30 165 30 1156 30 501 30 3948 Fragments arranged by size: 3948 1156 501 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5890 BseYI C'CCAG_C Cuts at: 2113 5093 5589 2113 Size: 2980 496 2414 Fragments arranged by size: 2980 2414 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3340 4101 5129 1540 Size: 275 186 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2095 3951 4036 5107 2095 Size: 1856 85 1071 2878 Fragments arranged by size: 2878 1856 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 3209 3951 4036 5107 1391 Size: 399 305 1114 742 85 1071 2174 Fragments arranged by size: 2174 1114 1071 742 399 305 85 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2042 3245 844 Size: 1198 1203 3489 Fragments arranged by size: 3489 1203 1198 BsrBI CCG'CTC Cuts at: 1394 1823 3777 1394 Size: 429 1954 3507 Fragments arranged by size: 3507 1954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2946 4385 4559 65 Size: 1183 1698 1439 174 1396 Fragments arranged by size: 1698 1439 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4534 4721 1700 Size: 110 2724 187 2869 Fragments arranged by size: 2869 2724 187 110 BsrGI T'GTAC_A Cuts at: 96 2392 96 Size: 2296 3594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 2046 3950 4962 2046 Size: 1904 1012 2974 Fragments arranged by size: 2974 1904 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5890 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5890 BstYI r'GATC_y Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885 Size: 1248 367 385 17 1452 86 12 346 Cuts at: 5885 1972 Size: 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5338 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2742 3337 4226 Size: 54 1425 50 275 18 912 595 889 Cuts at: 4226 5855 8 Size: 1629 43 Fragments arranged by size: 1629 1425 912 889 595 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2665 3033 3057 3820 1353 Size: 1312 368 24 763 3423 Fragments arranged by size: 3423 1312 763 368 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2093 2093 Size: 5890 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2583 2586 2709 2718 2973 3038 3152 Size: 393 951 3 123 9 255 65 114 Cuts at: 3152 3953 4550 5337 5605 1239 Size: 801 597 787 268 1524 Fragments arranged by size: 1524 951 801 787 597 393 268 255 123 114 65 9 3 EcoRI G'AATT_C Cuts at: 2841 3199 3577 2841 Size: 358 378 5154 Fragments arranged by size: 5154 378 358 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 5037 1678 Size: 3359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2308 2340 2982 3014 3090 Size: 32 135 32 736 32 642 32 76 Cuts at: 3090 3122 4531 4563 4605 4637 1373 Size: 32 1409 32 42 32 2626 Fragments arranged by size: 2626 1409 736 642 135 76 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 2030 748 Size: 1282 4608 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2894 3129 5004 5188 1305 Size: 1589 235 1875 184 2007 Fragments arranged by size: 2007 1875 1589 235 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2744 10 Size: 54 1425 343 912 3156 Fragments arranged by size: 3156 1425 912 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 3848 4207 4366 518 Size: 648 1731 951 359 159 2042 Fragments arranged by size: 2042 1731 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3064 3306 3983 5131 5376 1606 Size: 1458 242 677 1148 245 2120 Fragments arranged by size: 2120 1458 1148 677 245 242 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5338 NdeI CA'TA_TG Cuts at: 387 2232 387 Size: 1845 4045 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 2311 2537 3248 4793 1132 Size: 97 1082 226 711 1545 2229 Fragments arranged by size: 2229 1545 1082 711 226 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 2307 4789 2307 Size: 2482 3408 PflMI CCAn_nnn'nTGG Cuts at: 1108 2876 1108 Size: 1768 4122 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 2966 3460 2966 Size: 494 5396 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2628 2660 2628 Size: 32 5858 PstI C_TGCA'G Cuts at: 830 2767 3031 3242 830 Size: 1937 264 211 3478 Fragments arranged by size: 3478 1937 264 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 3064 3064 Size: 5890 SacI G_AGCT'C Cuts at: 2095 2095 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SapI GCTCTTCn'nnn_ Cuts at: 3057 3057 Size: 5890 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2103 2763 3027 3136 3238 4373 5054 Size: 219 1058 660 264 109 102 1135 681 Cuts at: 5054 5245 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1058 681 660 264 219 191 109 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3994 4895 5157 820 Size: 197 926 333 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 333 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 3134 1517 Size: 1617 4273 StyI C'CwwG_G Cuts at: 513 1065 2613 3329 513 Size: 552 1548 716 3074 Fragments arranged by size: 3074 1548 716 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2232 3917 3934 4087 4272 4717 325 Size: 1255 652 1685 17 153 185 445 1498 Fragments arranged by size: 1685 1498 1255 652 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2392 4136 5848 Size: 274 80 33 51 1443 415 1744 1712 Cuts at: 5848 96 Size: 138 Fragments arranged by size: 1744 1712 1443 415 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 5850 5882 2559 Size: 32 3259 32 2567 Fragments arranged by size: 3259 2567 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2209 2209 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 2659 3051 4019 2659 Size: 392 968 4530 Fragments arranged by size: 4530 968 392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI