(Circular) (Six-base) MAPSORT of: pFN21AB8940.seq Check: 7926 from: 1 to: 5596 pFN21AB8940 5596 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2620 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2152 2720 3659 5043 5311 1632 Size: 520 568 939 1384 268 1917 Fragments arranged by size: 1917 1384 939 568 520 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 2540 Size: 32 5564 AlwNI CAG_nnn'CTG Cuts at: 2272 2539 2575 3339 4911 2272 Size: 267 36 764 1572 2957 Fragments arranged by size: 2957 1572 764 267 36 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2396 2405 2921 1870 Size: 73 453 9 516 4545 Fragments arranged by size: 4545 516 453 73 9 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 2789 2804 2842 2917 Size: 325 217 627 440 562 15 38 75 Cuts at: 2917 4372 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 562 440 325 217 75 38 15 BanII G_rGCy'C Cuts at: 1391 2302 2396 2432 2468 2915 1391 Size: 911 94 36 36 447 4072 Fragments arranged by size: 4072 911 447 94 36 36 BbeI G_GCGC'C Cuts at: 2231 2846 2231 Size: 615 4981 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2096 2455 4207 Size: 107 122 71 631 605 424 359 1752 Cuts at: 4207 136 Size: 1525 Fragments arranged by size: 1752 1525 631 605 424 359 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 2462 2612 3013 2462 Size: 150 401 5045 Fragments arranged by size: 5045 401 150 Bme1580I G_kGCm'C Cuts at: 1790 3657 4813 1790 Size: 1867 1156 2573 Fragments arranged by size: 2573 1867 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 3327 4285 Size: 416 53 83 399 24 865 1045 958 Cuts at: 4285 442 Size: 1753 Fragments arranged by size: 1753 1045 958 865 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2567 2587 2711 4256 1239 Size: 1328 20 124 1545 2579 Fragments arranged by size: 2579 1545 1328 124 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 3721 4586 4884 2342 Size: 1379 865 298 3054 Fragments arranged by size: 3054 1379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2228 2277 2516 2843 Size: 53 83 186 936 695 49 239 327 Cuts at: 2843 3403 3785 4420 275 Size: 560 382 635 1451 Fragments arranged by size: 1451 936 695 635 560 382 327 239 186 83 53 49 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 1178 Size: 30 165 30 1137 30 4204 Fragments arranged by size: 4204 1137 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2345 1186 Size: 955 204 4437 Fragments arranged by size: 4437 955 204 BseYI C'CCAG_C Cuts at: 2084 2293 2470 4799 5295 2084 Size: 209 177 2329 496 2385 Fragments arranged by size: 2385 2329 496 209 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 2461 1163 Size: 573 528 197 4298 Fragments arranged by size: 4298 573 528 197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2327 2781 3046 3807 4835 1540 Size: 275 186 326 454 265 761 1028 2301 Fragments arranged by size: 2301 1028 761 454 326 275 265 186 BsiHKAI G_wGCw'C Cuts at: 3657 3742 4813 3657 Size: 85 1071 4440 Fragments arranged by size: 4440 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 BsmBI CGTCTCn'nnnn_ Cuts at: 2618 2618 Size: 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2302 2396 2432 2468 2915 3657 3742 Size: 399 512 94 36 36 447 742 85 Cuts at: 3742 4813 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 512 447 399 94 85 36 36 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 2951 844 Size: 1776 331 3489 Fragments arranged by size: 3489 1776 331 BsrBI CCG'CTC Cuts at: 1394 1823 2329 3483 1394 Size: 429 506 1154 3507 Fragments arranged by size: 3507 1154 506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2449 2728 4240 4427 1700 Size: 110 246 393 279 1512 187 2869 Fragments arranged by size: 2869 1512 393 279 246 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 2590 2824 1925 Size: 665 234 4697 Fragments arranged by size: 4697 665 234 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591 Size: 954 367 385 17 1452 86 12 346 Cuts at: 5591 1972 Size: 1977 Fragments arranged by size: 1977 1452 954 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2094 2100 2112 2321 2417 2510 2781 Size: 552 1029 6 12 209 96 93 271 Cuts at: 2781 2847 513 Size: 66 3262 Fragments arranged by size: 3262 1029 552 271 209 96 93 66 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2566 487 Size: 683 56 1340 3517 Fragments arranged by size: 3517 1340 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3906 3926 3112 Size: 794 20 4782 Fragments arranged by size: 4782 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DraIII CAC_nnn'GTG Cuts at: 2024 2024 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2187 2324 Size: 54 1425 50 275 18 267 90 137 Cuts at: 2324 2332 2451 2706 2778 3043 3932 5561 8 Size: 8 119 255 72 265 889 1629 43 Fragments arranged by size: 1629 1425 889 275 267 265 255 137 119 90 72 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2324 2778 3043 1537 Size: 275 512 454 265 4090 Fragments arranged by size: 4090 512 454 275 265 EarI CTCTTCn'nnn_ Cuts at: 1353 2320 3526 1353 Size: 967 1206 3423 Fragments arranged by size: 3423 1206 967 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2032 2549 4183 4557 4703 387 Size: 1064 138 443 517 1634 374 146 1280 Fragments arranged by size: 1634 1280 1064 517 443 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2567 2587 2711 2720 3659 4256 Size: 393 520 415 20 124 9 939 597 Cuts at: 4256 5043 5311 1239 Size: 787 268 1524 Fragments arranged by size: 1524 939 787 597 520 415 393 268 124 20 9 EcoO109I rG'GnC_Cy Cuts at: 2080 2412 2080 Size: 332 5264 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2091 2183 2223 2231 2546 2846 4743 1678 Size: 413 92 40 8 315 300 1897 2531 Fragments arranged by size: 2531 1897 413 315 300 92 40 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 4237 4269 4311 Size: 32 135 32 725 32 1908 32 42 Cuts at: 4311 4343 1373 Size: 32 2626 Fragments arranged by size: 2626 1908 725 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KasI G'GCGC_C Cuts at: 2227 2842 2227 Size: 615 4981 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3554 3913 4072 518 Size: 648 2388 359 159 2042 Fragments arranged by size: 2388 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2323 2512 2536 2771 2783 3012 3689 Size: 490 227 189 24 235 12 229 677 Cuts at: 3689 4837 5082 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 490 245 235 229 227 189 24 12 NaeI GCC'GGC Cuts at: 2058 2451 2058 Size: 393 5203 NarI GG'CG_CC Cuts at: 2228 2843 2228 Size: 615 4981 NcoI C'CATG_G Cuts at: 513 1065 2112 513 Size: 552 1047 3997 Fragments arranged by size: 3997 1047 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NgoMIV G'CCGG_C Cuts at: 2056 2449 2056 Size: 393 5203 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 2324 2778 3043 2324 Size: 454 265 4877 Fragments arranged by size: 4877 454 265 NspI r_CATG'y Cuts at: 1132 1229 2884 2954 4499 1132 Size: 97 1655 70 1545 2229 Fragments arranged by size: 2229 1655 1545 97 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2540 2572 3665 3697 5178 5210 2540 Size: 32 1093 32 1481 32 2926 Fragments arranged by size: 2926 1481 1093 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2412 2412 Size: 5596 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2536 2771 2536 Size: 235 5361 SacII CC_GC'GG Cuts at: 2097 2324 2513 2784 2097 Size: 227 189 271 4909 Fragments arranged by size: 4909 271 227 189 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2772 2875 2944 4079 4760 4951 826 Size: 219 1727 103 69 1135 681 191 1471 Fragments arranged by size: 1727 1471 1135 681 219 191 103 69 SfoI GGC'GCC Cuts at: 2229 2844 2229 Size: 615 4981 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2357 3700 4601 4863 820 Size: 197 926 414 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 414 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2112 2211 2661 3035 513 Size: 552 1047 99 450 374 3074 Fragments arranged by size: 3074 1047 552 450 374 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2262 2294 5556 5588 2262 Size: 32 3262 32 2270 Fragments arranged by size: 3262 2270 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI