(Linear) (Six-base) MAPSORT of: KIEE2557.seq Check: 5207 from: 1 to: 2841 KIEE2557.orf With 182 enzymes: SgfI * December 15, 2014 12:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1440 2841 Size: 1440 1401 AatII G_ACGT'C Cuts at: 0 1274 2841 Size: 1274 1567 AccI GT'mk_AC Cuts at: 0 2662 2841 Size: 2662 179 Acc65I G'GTAC_C Cuts at: 0 1593 2841 Size: 1593 1248 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 601 739 844 2243 2272 2841 Size: 45 556 138 105 1399 29 569 Fragments arranged by size: 1399 569 556 138 105 45 29 AflII C'TTAA_G Cuts at: 0 2527 2841 Size: 2527 314 AhdI GACnn_n'nnGTC Cuts at: 0 912 2841 Size: 912 1929 AleI CACnn'nnGTG Cuts at: 0 1432 2841 Size: 1432 1409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 2108 2841 Size: 295 1813 733 Fragments arranged by size: 1813 733 295 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 413 445 2841 Size: 413 32 2396 Fragments arranged by size: 2396 413 32 AlwNI CAG_nnn'CTG Cuts at: 0 1191 1510 2841 Size: 1191 319 1331 Fragments arranged by size: 1331 1191 319 ApaI G_GGCC'C Cuts at: 0 224 406 1981 2756 2841 Size: 224 182 1575 775 85 Fragments arranged by size: 1575 775 224 182 85 ApaLI G'TGCA_C Cuts at: 0 1081 2841 Size: 1081 1760 ApoI r'AATT_y Cuts at: 0 2431 2709 2841 Size: 2431 278 132 Fragments arranged by size: 2431 278 132 AvaI C'yCGr_G Cuts at: 0 47 233 1265 2561 2841 Size: 47 186 1032 1296 280 Fragments arranged by size: 1296 1032 280 186 47 BanI G'GyrC_C Cuts at: 0 918 1260 1369 1411 1593 2841 Size: 918 342 109 42 182 1248 Fragments arranged by size: 1248 918 342 182 109 42 BanII G_rGCy'C Cuts at: 0 224 406 1981 2302 2756 2840 2841 Size: 224 182 1575 321 454 84 1 Fragments arranged by size: 1575 454 321 224 182 84 1 BbsI GAAGACnn'nnnn_ Cuts at: 0 383 2841 Size: 383 2458 BciVI GTATCCnnnnn_n' Cuts at: 0 1921 2841 Size: 1921 920 BclI T'GATC_A Cuts at: 0 1740 2841 Size: 1740 1101 BglI GCCn_nnn'nGGC Cuts at: 0 1985 2841 Size: 1985 856 BglII A'GATC_T Cuts at: 0 158 2841 Size: 158 2683 BlpI GC'TnA_GC Cuts at: 0 1111 2734 2841 Size: 1111 1623 107 Fragments arranged by size: 1623 1111 107 Bme1580I G_kGCm'C Cuts at: 0 224 406 923 1085 1212 1372 1981 2756 Size: 224 182 517 162 127 160 609 775 Cuts at: 2756 2841 Size: 85 Fragments arranged by size: 775 609 517 224 182 162 160 127 85 BmgBI CAC'GTC Cuts at: 0 84 2841 Size: 84 2757 BmrI ACTGGGnnnn_n' Cuts at: 0 306 351 409 1632 1722 1933 1992 2841 Size: 306 45 58 1223 90 211 59 849 Fragments arranged by size: 1223 849 306 211 90 59 58 45 BmtI G_CTAG'C Cuts at: 0 154 2841 Size: 154 2687 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 832 889 931 988 998 1234 1342 Size: 706 126 57 42 57 10 236 108 Cuts at: 1342 1391 1960 2656 2841 Size: 49 569 696 185 Fragments arranged by size: 706 696 569 236 185 126 108 57 57 49 42 10 Bpu10I CC'TnA_GC Cuts at: 0 671 1132 2296 2841 Size: 671 461 1164 545 Fragments arranged by size: 1164 671 545 461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1285 2841 Size: 1285 1556 BsaI GGTCTCn'nnnn_ Cuts at: 0 651 1725 1862 2036 2841 Size: 651 1074 137 174 805 Fragments arranged by size: 1074 805 651 174 137 BsaHI Gr'CG_yC Cuts at: 0 1271 2315 2841 Size: 1271 1044 526 Fragments arranged by size: 1271 1044 526 BsaWI w'CCGG_w Cuts at: 0 616 1313 2330 2841 Size: 616 697 1017 511 Fragments arranged by size: 1017 697 616 511 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2809 2839 2841 Size: 2809 30 2 Fragments arranged by size: 2809 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 117 2262 2841 Size: 117 2145 579 Fragments arranged by size: 2145 579 117 BseYI C'CCAG_C Cuts at: 0 74 114 462 486 702 806 1573 1881 Size: 74 40 348 24 216 104 767 308 Cuts at: 1881 2126 2841 Size: 245 715 Fragments arranged by size: 767 715 348 308 245 216 104 74 40 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 557 847 2515 2841 Size: 557 290 1668 326 Fragments arranged by size: 1668 557 326 290 BsiEI CG_ry'CG Cuts at: 0 72 1314 2841 Size: 72 1242 1527 Fragments arranged by size: 1527 1242 72 BsiHKAI G_wGCw'C Cuts at: 0 476 1085 2840 2841 Size: 476 609 1755 1 Fragments arranged by size: 1755 609 476 1 BsmI GAATG_Cn' Cuts at: 0 418 2841 Size: 418 2423 Bsp1286I G_dGCh'C Cuts at: 0 224 406 476 923 1085 1212 1372 1981 Size: 224 182 70 447 162 127 160 609 Cuts at: 1981 2302 2756 2840 2841 Size: 321 454 84 1 Fragments arranged by size: 609 454 447 321 224 182 162 160 127 84 70 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1440 1703 2702 2841 Size: 1440 263 999 139 Fragments arranged by size: 1440 999 263 139 BsrBI CCG'CTC Cuts at: 0 1944 2784 2841 Size: 1944 840 57 Fragments arranged by size: 1944 840 57 BsrDI GCAATG_nn' Cuts at: 0 530 2841 Size: 530 2311 BsrFI r'CCGG_y Cuts at: 0 517 1414 1708 2841 Size: 517 897 294 1133 Fragments arranged by size: 1133 897 517 294 BsrGI T'GTAC_A Cuts at: 0 1967 2841 Size: 1967 874 BstAPI GCAn_nnn'nTGC Cuts at: 0 1857 2841 Size: 1857 984 BstEII G'GTnAC_C Cuts at: 0 630 2841 Size: 630 2211 BstXI CCAn_nnnn'nTGG Cuts at: 0 450 1918 2046 2841 Size: 450 1468 128 795 Fragments arranged by size: 1468 795 450 128 BstYI r'GATC_y Cuts at: 0 158 2502 2841 Size: 158 2344 339 Fragments arranged by size: 2344 339 158 BstZ17I GTA'TAC Cuts at: 0 2663 2841 Size: 2663 178 Bsu36I CC'TnA_GG Cuts at: 0 289 730 782 1598 2748 2841 Size: 289 441 52 816 1150 93 Fragments arranged by size: 1150 816 441 289 93 52 BtsI GCAGTG_nn' Cuts at: 0 145 773 1703 1776 1933 2841 Size: 145 628 930 73 157 908 Fragments arranged by size: 930 908 628 157 145 73 ClaI AT'CG_AT Cuts at: 0 1763 2841 Size: 1763 1078 DraIII CAC_nnn'GTG Cuts at: 0 1089 2841 Size: 1089 1752 EaeI y'GGCC_r Cuts at: 0 1773 2841 Size: 1773 1068 EarI CTCTTCn'nnn_ Cuts at: 0 340 2219 2841 Size: 340 1879 622 Fragments arranged by size: 1879 622 340 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 244 320 2841 Size: 244 76 2521 Fragments arranged by size: 2521 244 76 EcoICRI GAG'CTC Cuts at: 0 2838 2841 Size: 2838 3 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 601 706 739 832 844 889 931 Size: 45 556 105 33 93 12 45 42 Cuts at: 931 988 998 1234 1342 1391 1960 2243 2272 Size: 57 10 236 108 49 569 283 29 Cuts at: 2272 2656 2841 Size: 384 185 Fragments arranged by size: 569 556 384 283 236 185 108 105 93 57 49 45 45 42 33 29 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 2698 2776 2841 Size: 2698 78 65 Fragments arranged by size: 2698 78 65 EcoO109I rG'GnC_Cy Cuts at: 0 220 221 403 490 933 1056 1885 1977 Size: 220 1 182 87 443 123 829 92 Cuts at: 1977 1978 2571 2752 2753 2799 2841 Size: 1 593 181 1 46 42 Fragments arranged by size: 829 593 443 220 182 181 123 92 87 46 42 1 1 1 EcoRI G'AATT_C Cuts at: 0 2431 2841 Size: 2431 410 EcoRV GAT'ATC Cuts at: 0 126 894 2841 Size: 126 768 1947 Fragments arranged by size: 1947 768 126 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 462 1131 1185 2841 Size: 462 669 54 1656 Fragments arranged by size: 1656 669 462 54 HaeII r_GCGC'y Cuts at: 0 703 1230 2841 Size: 703 527 1611 Fragments arranged by size: 1611 703 527 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 9 41 898 930 2841 Size: 9 32 857 32 1911 Fragments arranged by size: 1911 857 32 32 9 KpnI G_GTAC'C Cuts at: 0 1597 2841 Size: 1597 1244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1523 2841 Size: 1523 1318 MscI TGG'CCA Cuts at: 0 1775 2841 Size: 1775 1066 MslI CAynn'nnrTG Cuts at: 0 1432 1588 2584 2762 2841 Size: 1432 156 996 178 79 Fragments arranged by size: 1432 996 178 156 79 MspA1I CmG'CkG Cuts at: 0 74 579 738 810 1191 1278 1341 1854 Size: 74 505 159 72 381 87 63 513 Cuts at: 1854 1881 2126 2499 2841 Size: 27 245 373 342 Fragments arranged by size: 513 505 381 373 342 245 159 87 74 72 63 27 NaeI GCC'GGC Cuts at: 0 519 1710 2841 Size: 519 1191 1131 Fragments arranged by size: 1191 1131 519 NgoMIV G'CCGG_C Cuts at: 0 517 1708 2841 Size: 517 1191 1133 Fragments arranged by size: 1191 1133 517 NheI G'CTAG_C Cuts at: 0 150 2841 Size: 150 2691 PflMI CCAn_nnn'nTGG Cuts at: 0 929 1482 1782 2841 Size: 929 553 300 1059 Fragments arranged by size: 1059 929 553 300 PfoI T'CCnGG_A Cuts at: 0 975 1842 2045 2633 2841 Size: 975 867 203 588 208 Fragments arranged by size: 975 867 588 208 203 PpuMI rG'GwC_Cy Cuts at: 0 490 933 1056 2841 Size: 490 443 123 1785 Fragments arranged by size: 1785 490 443 123 PshAI GACnn'nnGTC Cuts at: 0 49 1316 2841 Size: 49 1267 1525 Fragments arranged by size: 1525 1267 49 PspOMI G'GGCC_C Cuts at: 0 220 402 1977 2752 2841 Size: 220 182 1575 775 89 Fragments arranged by size: 1575 775 220 182 89 PstI C_TGCA'G Cuts at: 0 881 1346 1673 2087 2841 Size: 881 465 327 414 754 Fragments arranged by size: 881 754 465 414 327 PvuII CAG'CTG Cuts at: 0 579 738 810 1341 1854 1881 2499 2841 Size: 579 159 72 531 513 27 618 342 Fragments arranged by size: 618 579 531 513 342 159 72 27 SacI G_AGCT'C Cuts at: 0 2840 2841 Size: 2840 1 SanDI GG'GwC_CC Cuts at: 0 933 2841 Size: 933 1908 SfcI C'TryA_G Cuts at: 0 877 1342 1669 1805 2025 2083 2841 Size: 877 465 327 136 220 58 758 Fragments arranged by size: 877 758 465 327 220 136 58 SmaI CCC'GGG Cuts at: 0 2563 2841 Size: 2563 278 SmlI C'TyrA_G Cuts at: 0 1300 2527 2841 Size: 1300 1227 314 Fragments arranged by size: 1300 1227 314 StuI AGG'CCT Cuts at: 0 2089 2841 Size: 2089 752 StyI C'CwwG_G Cuts at: 0 2436 2567 2841 Size: 2436 131 274 Fragments arranged by size: 2436 274 131 TatI w'GTAC_w Cuts at: 0 1967 2841 Size: 1967 874 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 183 215 611 643 1362 1394 2809 2841 Size: 183 32 396 32 719 32 1415 32 Fragments arranged by size: 1415 719 396 183 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1102 2841 Size: 1102 1739 XmaI C'CCGG_G Cuts at: 0 2561 2841 Size: 2561 280 XmnI GAAnn'nnTTC Cuts at: 0 2629 2841 Size: 2629 212 ZraI GAC'GTC Cuts at: 0 1272 2841 Size: 1272 1569 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NheI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SfcI SmaI SmlI StuI StyI TatI TstI Tth111I XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflIII AgeI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BplI BsaAI BsaBI BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI BtgI BtgZI CspCI DraI DrdI EagI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI XhoI