(Circular) (Six-base) MAPSORT of: pFN21AB7888.seq Check: 2280 from: 1 to: 6658 pFN21AB7888 6658 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4468 278 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3952 4001 1937 Size: 2015 49 4594 Fragments arranged by size: 4594 2015 49 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 4785 5158 1563 Size: 3222 373 3063 Fragments arranged by size: 3222 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2134 2736 3640 4721 6105 6373 1632 Size: 502 602 904 1081 1384 268 1917 Fragments arranged by size: 1917 1384 1081 904 602 502 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6461 AflIII A'CryG_T Cuts at: 1203 5557 6534 1203 Size: 4354 977 1327 Fragments arranged by size: 4354 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 5387 5387 Size: 6658 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6658 AlwNI CAG_nnn'CTG Cuts at: 2122 4401 5973 2122 Size: 2279 1572 2807 Fragments arranged by size: 2807 2279 1572 ApaLI G'TGCA_C Cuts at: 3195 4715 5871 3195 Size: 1520 1156 3982 Fragments arranged by size: 3982 1520 1156 ApoI r'AATT_y Cuts at: 1493 2264 2372 2610 2742 2922 3071 3904 3967 Size: 771 108 238 132 180 149 833 63 Cuts at: 3967 4193 4345 6454 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 833 771 238 226 180 152 149 132 108 63 AseI AT'TA_AT Cuts at: 160 1032 3258 5212 160 Size: 872 2226 1954 1606 Fragments arranged by size: 2226 1954 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 2940 3983 1870 Size: 73 997 1043 4545 Fragments arranged by size: 4545 1043 997 73 BamHI G'GATC_C Cuts at: 3373 3988 4355 3373 Size: 615 367 5676 Fragments arranged by size: 5676 615 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2219 3979 5434 618 Size: 325 217 627 294 138 1760 1455 1842 Fragments arranged by size: 1842 1760 1455 627 325 294 217 138 BanII G_rGCy'C Cuts at: 1391 2168 3977 1391 Size: 777 1809 4072 Fragments arranged by size: 4072 1809 777 BbeI G_GCGC'C Cuts at: 2223 2223 Size: 6658 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5687 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4849 4883 1903 Size: 34 2912 34 3678 Fragments arranged by size: 3678 2912 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4550 5760 4550 Size: 1210 5448 BclI T'GATC_A Cuts at: 1548 1548 Size: 6658 BfrBI ATG'CAT Cuts at: 3701 3701 Size: 6658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2079 5269 136 Size: 107 122 71 631 605 407 3190 1525 Fragments arranged by size: 3190 1525 631 605 407 122 107 71 BglII A'GATC_T Cuts at: 2620 2689 6653 2620 Size: 69 3964 2625 Fragments arranged by size: 3964 2625 69 BlpI GC'TnA_GC Cuts at: 3355 4075 3355 Size: 720 5938 Bme1580I G_kGCm'C Cuts at: 1790 2320 3199 4719 5875 1790 Size: 530 879 1520 1156 2573 Fragments arranged by size: 2573 1520 1156 879 530 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4389 5347 442 Size: 416 53 83 399 24 2972 958 1753 Fragments arranged by size: 2972 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 2784 5318 1239 Size: 1248 297 2534 2579 Fragments arranged by size: 2579 2534 1248 297 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3184 3219 4783 5648 5946 3184 Size: 35 1564 865 298 3896 Fragments arranged by size: 3896 1564 865 298 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5321 882 Size: 617 3822 2219 Fragments arranged by size: 3822 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4449 493 Size: 711 3245 2702 Fragments arranged by size: 3245 2702 711 BsaBI GATnn'nnATC Cuts at: 2033 2694 6652 2033 Size: 661 3958 2039 Fragments arranged by size: 3958 2039 661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2220 4465 4847 5482 Size: 53 83 186 936 687 2245 382 635 Cuts at: 5482 275 Size: 1451 Fragments arranged by size: 2245 1451 936 687 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3094 5091 5489 5763 5910 6321 3094 Size: 1997 398 274 147 411 3431 Fragments arranged by size: 3431 1997 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6433 Fragments arranged by size: 6433 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2189 2192 1186 Size: 1000 3 3 5652 Fragments arranged by size: 5652 1000 3 3 BseYI C'CCAG_C Cuts at: 2136 5861 6357 2136 Size: 3725 496 2437 Fragments arranged by size: 3725 2437 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2658 3921 1163 Size: 573 922 1263 3900 Fragments arranged by size: 3900 1263 922 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4108 4869 5897 1540 Size: 275 186 2107 761 1028 2301 Fragments arranged by size: 2301 2107 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3199 4719 4804 5875 3199 Size: 1520 85 1071 3982 Fragments arranged by size: 3982 1520 1071 85 BsmI GAATG_Cn' Cuts at: 4169 4262 4169 Size: 93 6565 BsmBI CGTCTCn'nnnn_ Cuts at: 2081 2081 Size: 6658 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2320 3199 3977 4719 4804 5875 Size: 399 378 152 879 778 742 85 1071 Cuts at: 5875 1391 Size: 2174 Fragments arranged by size: 2174 1071 879 778 742 399 378 152 85 BspEI T'CCGG_A Cuts at: 3094 6321 3094 Size: 3227 3431 BspHI T'CATG_A Cuts at: 3174 4547 6277 3174 Size: 1373 1730 3555 Fragments arranged by size: 3555 1730 1373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2047 2103 4013 844 Size: 1203 56 1910 3489 Fragments arranged by size: 3489 1910 1203 56 BsrBI CCG'CTC Cuts at: 1394 1823 4545 1394 Size: 429 2722 3507 Fragments arranged by size: 3507 2722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3095 5153 5327 65 Size: 1183 1847 2058 174 1396 Fragments arranged by size: 2058 1847 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5302 5489 1700 Size: 110 3492 187 2869 Fragments arranged by size: 3492 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3517 96 Size: 3421 3237 BssHII G'CGCG_C Cuts at: 1925 2017 1925 Size: 92 6566 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 3127 794 Size: 2333 4325 BstBI TT'CG_AA Cuts at: 2593 5462 2593 Size: 2869 3789 BstEII G'GTnAC_C Cuts at: 1184 2810 1184 Size: 1626 5032 BstXI CCAn_nnnn'nTGG Cuts at: 3634 3715 3634 Size: 81 6577 BstYI r'GATC_y Cuts at: 1972 2620 2642 2689 3373 3988 4355 4740 4757 Size: 648 22 47 684 615 367 385 17 Cuts at: 4757 6209 6295 6307 6653 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 684 648 615 385 367 346 86 47 22 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5919 Fragments arranged by size: 5919 683 56 BtsI GCAGTG_nn' Cuts at: 2028 4174 4968 4988 2028 Size: 2146 794 20 3698 Fragments arranged by size: 3698 2146 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6623 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DraIII CAC_nnn'GTG Cuts at: 2980 2980 Size: 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3228 5665 809 Size: 507 1912 2437 1802 Fragments arranged by size: 2437 1912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2213 3205 4105 Size: 54 1425 50 275 18 383 992 900 Cuts at: 4105 4994 6623 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 992 900 889 383 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4105 1537 Size: 275 2293 4090 Fragments arranged by size: 4090 2293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 3526 4588 1353 Size: 1484 689 1062 3423 Fragments arranged by size: 3423 1484 1062 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5245 5619 5765 387 Size: 1064 138 3656 374 146 1280 Fragments arranged by size: 3656 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2487 2736 2784 3640 4721 5318 Size: 393 502 353 249 48 856 1081 597 Cuts at: 5318 6105 6373 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1081 856 787 597 502 393 353 268 249 48 EcoO109I rG'GnC_Cy Cuts at: 3230 3230 Size: 6658 EcoRI G'AATT_C Cuts at: 3967 4345 3967 Size: 378 6280 EcoRV GAT'ATC Cuts at: 2445 2445 Size: 6658 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3216 3216 Size: 6658 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 2223 5805 1678 Size: 545 3582 2531 Fragments arranged by size: 3582 2531 545 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2371 2378 2403 2410 2986 Size: 32 135 32 799 7 25 7 576 Cuts at: 2986 3018 3047 3079 3169 3201 3373 3405 5299 Size: 32 29 32 90 32 172 32 1894 Cuts at: 5299 5331 5373 5405 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1894 799 576 172 135 90 42 32 32 32 32 32 32 32 32 29 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2827 4002 4248 669 Size: 988 281 889 1175 246 3079 Fragments arranged by size: 3079 1175 988 889 281 246 HindIII A'AGCT_T Cuts at: 748 3237 748 Size: 2489 4169 HpaI GTT'AAC Cuts at: 2827 4248 2827 Size: 1421 5237 KasI G'GCGC_C Cuts at: 2219 2219 Size: 6658 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 2438 3485 4257 2438 Size: 1047 772 4839 Fragments arranged by size: 4839 1047 772 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2581 5772 5956 1305 Size: 1276 3191 184 2007 Fragments arranged by size: 3191 2007 1276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3207 10 Size: 54 1425 343 1375 3461 Fragments arranged by size: 3461 1425 1375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3108 3176 3525 3713 3858 4616 Size: 648 1815 127 68 349 188 145 758 Cuts at: 4616 4975 5134 518 Size: 359 159 2042 Fragments arranged by size: 2042 1815 758 648 359 349 188 159 145 127 68 MspA1I CmG'CkG Cuts at: 1606 2104 2140 4074 4751 5899 6144 1606 Size: 498 36 1934 677 1148 245 2120 Fragments arranged by size: 2120 1934 1148 677 498 245 36 NarI GG'CG_CC Cuts at: 2220 2220 Size: 6658 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6106 NdeI CA'TA_TG Cuts at: 387 387 Size: 6658 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 4105 4105 Size: 6658 NsiI A_TGCA'T Cuts at: 3703 3703 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 3611 4016 5561 1132 Size: 97 2382 405 1545 2229 Fragments arranged by size: 2382 2229 1545 405 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6658 PciI A'CATG_T Cuts at: 5557 5557 Size: 6658 PflMI CCAn_nnn'nTGG Cuts at: 1108 2068 1108 Size: 960 5698 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3230 3230 Size: 6658 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6658 PsiI TTA'TAA Cuts at: 3915 4228 3915 Size: 313 6345 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3597 3629 3597 Size: 32 6626 PstI C_TGCA'G Cuts at: 830 4010 830 Size: 3180 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 SalI G'TCGA_C Cuts at: 1936 4000 1936 Size: 2064 4594 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 3648 4906 6618 3648 Size: 1258 1712 3688 Fragments arranged by size: 3688 1712 1258 SexAI A'CCwGG_T Cuts at: 2860 3600 2860 Size: 740 5918 SfcI C'TryA_G Cuts at: 826 1045 2629 3566 3681 4006 5141 5822 6013 Size: 219 1584 937 115 325 1135 681 191 Cuts at: 6013 826 Size: 1471 Fragments arranged by size: 1584 1471 1135 937 681 325 219 191 115 SfoI GGC'GCC Cuts at: 2221 2221 Size: 6658 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 3985 1872 Size: 2113 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3163 3234 4762 5663 5925 820 Size: 197 926 1220 71 1528 901 262 1553 Fragments arranged by size: 1553 1528 1220 926 901 262 197 71 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 3955 152 Size: 3803 2855 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 3493 4582 5 Size: 47 3441 1089 2081 Fragments arranged by size: 3441 2081 1089 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6658 StyI C'CwwG_G Cuts at: 513 1065 2285 3627 4097 513 Size: 552 1220 1342 470 3074 Fragments arranged by size: 3074 1342 1220 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 4685 4702 4855 5040 5485 325 Size: 1255 627 2478 17 153 185 445 1498 Fragments arranged by size: 2478 1498 1255 627 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3517 3646 4904 Size: 274 80 33 51 1443 1540 129 1258 Cuts at: 4904 6616 96 Size: 1712 138 Fragments arranged by size: 1712 1540 1443 1258 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6618 6650 6618 Size: 32 6626 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6658 XbaI T'CTAG_A Cuts at: 3994 3994 Size: 6658 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 3983 1870 Size: 2113 4545 XmnI GAAnn'nnTTC Cuts at: 2949 3282 4787 2949 Size: 333 1505 4820 Fragments arranged by size: 4820 1505 333 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4466 276 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI