Restriction map for
pFN21AA0561
(Linear) (Six-base) MAPSORT of: KIAA0561.seq Check: 5597 from: 1 to: 3927
KIAA0561.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 893 1605 3642 3927
Size: 893 712 2037 285
Fragments arranged by size:
2037 893 712 285
AatII G_ACGT'C
Cuts at: 0 2927 3056 3927
Size: 2927 129 871
Fragments arranged by size:
2927 871 129
AccI GT'mk_AC
Cuts at: 0 1142 2120 2906 2927 3927
Size: 1142 978 786 21 1000
Fragments arranged by size:
1142 1000 978 786 21
AclI AA'CG_TT
Cuts at: 0 2019 3927
Size: 2019 1908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 2163 2868 3091 3927
Size: 1387 776 705 223 836
Fragments arranged by size:
1387 836 776 705 223
AfeI AGC'GCT
Cuts at: 0 1174 3927
Size: 1174 2753
AflIII A'CryG_T
Cuts at: 0 521 2008 3927
Size: 521 1487 1919
Fragments arranged by size:
1919 1487 521
AgeI A'CCGG_T
Cuts at: 0 2105 3927
Size: 2105 1822
AleI CACnn'nnGTG
Cuts at: 0 2467 3859 3927
Size: 2467 1392 68
Fragments arranged by size:
2467 1392 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1193 3927
Size: 1193 2734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2773 2805 3927
Size: 2773 32 1122
Fragments arranged by size:
2773 1122 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1036 2404 2620 2692 3379 3454 3927
Size: 1036 1368 216 72 687 75 473
Fragments arranged by size:
1368 1036 687 473 216 75 72
ApaI G_GGCC'C
Cuts at: 0 1381 2605 3136 3914 3927
Size: 1381 1224 531 778 13
Fragments arranged by size:
1381 1224 778 531 13
ApaLI G'TGCA_C
Cuts at: 0 1459 2935 3043 3927
Size: 1459 1476 108 884
Fragments arranged by size:
1476 1459 884 108
ApoI r'AATT_y
Cuts at: 0 189 213 874 3927
Size: 189 24 661 3053
Fragments arranged by size:
3053 661 189 24
AvaI C'yCGr_G
Cuts at: 0 326 471 665 1595 1645 1753 2305 2484
Size: 326 145 194 930 50 108 552 179
Cuts at: 2484 3423 3616 3907 3927
Size: 939 193 291 20
Fragments arranged by size:
939 930 552 326 291 194 193 179
145 108 50 20
BamHI G'GATC_C
Cuts at: 0 2275 3927
Size: 2275 1652
BanI G'GyrC_C
Cuts at: 0 23 601 996 1154 1310 1888 2545 3620
Size: 23 578 395 158 156 578 657 1075
Cuts at: 3620 3927
Size: 307
Fragments arranged by size:
1075 657 578 578 395 307 158 156
23
BanII G_rGCy'C
Cuts at: 0 35 1381 1864 2038 2605 2755 3136 3430
Size: 35 1346 483 174 567 150 381 294
Cuts at: 3430 3743 3914 3927
Size: 313 171 13
Fragments arranged by size:
1346 567 483 381 313 294 174 171
150 35 13
BbeI G_GCGC'C
Cuts at: 0 27 605 1314 2549 3927
Size: 27 578 709 1235 1378
Fragments arranged by size:
1378 1235 709 578 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 2192 2418 3927
Size: 2192 226 1509
Fragments arranged by size:
2192 1509 226
BbvCI CC'TCA_GC
Cuts at: 0 899 2254 3927
Size: 899 1355 1673
Fragments arranged by size:
1673 1355 899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 2488 2522 2523 2557 3927
Size: 34 2454 34 1 34 1370
Fragments arranged by size:
2454 1370 34 34 34 1
BclI T'GATC_A
Cuts at: 0 818 3927
Size: 818 3109
BglI GCCn_nnn'nGGC
Cuts at: 0 232 610 840 1135 2033 2408 2600 2980
Size: 232 378 230 295 898 375 192 380
Cuts at: 2980 3320 3927
Size: 340 607
Fragments arranged by size:
898 607 380 378 375 340 295 232
230 192
BglII A'GATC_T
Cuts at: 0 3233 3927
Size: 3233 694
BlpI GC'TnA_GC
Cuts at: 0 46 3927
Size: 46 3881
Bme1580I G_kGCm'C
Cuts at: 0 1381 1463 1882 1970 2605 2681 2939 3047
Size: 1381 82 419 88 635 76 258 108
Cuts at: 3047 3136 3623 3685 3914 3927
Size: 89 487 62 229 13
Fragments arranged by size:
1381 635 487 419 258 229 108 89
88 82 76 62 13
BmgBI CAC'GTC
Cuts at: 0 67 2943 3927
Size: 67 2876 984
Fragments arranged by size:
2876 984 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 1941 2079 2461 3854 3927
Size: 1941 138 382 1393 73
Fragments arranged by size:
1941 1393 382 138 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3135 3927
Size: 3135 792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 757 868 877 910 1006 1453 2625
Size: 19 738 111 9 33 96 447 1172
Cuts at: 2625 2970 3130 3414 3927
Size: 345 160 284 513
Fragments arranged by size:
1172 738 513 447 345 284 160 111
96 33 19 9
Bpu10I CC'TnA_GC
Cuts at: 0 899 2254 3927
Size: 899 1355 1673
Fragments arranged by size:
1673 1355 899
BsaI GGTCTCn'nnnn_
Cuts at: 0 1096 1297 2044 2603 2807 3193 3927
Size: 1096 201 747 559 204 386 734
Fragments arranged by size:
1096 747 734 559 386 204 201
BsaAI yAC'GTr
Cuts at: 0 522 1338 3927
Size: 522 816 2589
Fragments arranged by size:
2589 816 522
BsaBI GATnn'nnATC
Cuts at: 0 823 3927
Size: 823 3104
BsaHI Gr'CG_yC
Cuts at: 0 24 361 602 626 1311 1592 1627 1700
Size: 24 337 241 24 685 281 35 73
Cuts at: 1700 1826 2546 2924 3053 3927
Size: 126 720 378 129 874
Fragments arranged by size:
874 720 685 378 337 281 241 129
126 73 35 24 24
BsaWI w'CCGG_w
Cuts at: 0 806 2105 3280 3916 3927
Size: 806 1299 1175 636 11
Fragments arranged by size:
1299 1175 806 636 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 428 458 2166 2185 2196 2215 3325 3355
Size: 428 30 1708 19 11 19 1110 30
Cuts at: 3355 3927
Size: 572
Fragments arranged by size:
1708 1110 572 428 30 30 19 19
11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 793 1003 2082 2209 2628 3198 3267 3850
Size: 793 210 1079 127 419 570 69 583
Cuts at: 3850 3927
Size: 77
Fragments arranged by size:
1079 793 583 570 419 210 127 77
69
BseYI C'CCAG_C
Cuts at: 0 295 963 1029 1970 2300 2317 2613 2995
Size: 295 668 66 941 330 17 296 382
Cuts at: 2995 3033 3346 3372 3387 3412 3447 3472 3678
Size: 38 313 26 15 25 35 25 206
Cuts at: 3678 3927
Size: 249
Fragments arranged by size:
941 668 382 330 313 296 295 249
206 66 38 35 26 25 25 17
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 3241 3466 3745 3826 3927
Size: 358 2883 225 279 81 101
Fragments arranged by size:
2883 358 279 225 101 81
BsiEI CG_ry'CG
Cuts at: 0 432 2583 3927
Size: 432 2151 1344
Fragments arranged by size:
2151 1344 432
BsiHKAI G_wGCw'C
Cuts at: 0 1463 2939 3047 3430 3743 3927
Size: 1463 1476 108 383 313 184
Fragments arranged by size:
1476 1463 383 313 184 108
BsmBI CGTCTCn'nnnn_
Cuts at: 0 266 340 1411 3927
Size: 266 74 1071 2516
Fragments arranged by size:
2516 1071 266 74
Bsp1286I G_dGCh'C
Cuts at: 0 35 1381 1463 1864 1882 1970 2038 2605
Size: 35 1346 82 401 18 88 68 567
Cuts at: 2605 2681 2755 2939 3047 3136 3430 3623 3685
Size: 76 74 184 108 89 294 193 62
Cuts at: 3685 3743 3914 3927
Size: 58 171 13
Fragments arranged by size:
1346 567 401 294 193 184 171 108
89 88 82 76 74 68 62 58
35 18 13
BspEI T'CCGG_A
Cuts at: 0 806 3280 3927
Size: 806 2474 647
Fragments arranged by size:
2474 806 647
BspHI T'CATG_A
Cuts at: 0 509 1547 2747 3927
Size: 509 1038 1200 1180
Fragments arranged by size:
1200 1180 1038 509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 893 1221 1448 1605 3642 3927
Size: 893 328 227 157 2037 285
Fragments arranged by size:
2037 893 328 285 227 157
BsrBI CCG'CTC
Cuts at: 0 455 741 2310 3077 3599 3693 3927
Size: 455 286 1569 767 522 94 234
Fragments arranged by size:
1569 767 522 455 286 234 94
BsrFI r'CCGG_y
Cuts at: 0 431 2105 2231 2578 3927
Size: 431 1674 126 347 1349
Fragments arranged by size:
1674 1349 431 347 126
BssSI C'ACGA_G
Cuts at: 0 3722 3742 3927
Size: 3722 20 185
Fragments arranged by size:
3722 185 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1292 3927
Size: 1292 2635
BstEII G'GTnAC_C
Cuts at: 0 434 3927
Size: 434 3493
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1669 1685 2346 2866 3108 3129 3927
Size: 1669 16 661 520 242 21 798
Fragments arranged by size:
1669 798 661 520 242 21 16
BstYI r'GATC_y
Cuts at: 0 1223 2078 2275 3233 3927
Size: 1223 855 197 958 694
Fragments arranged by size:
1223 958 855 694 197
Bsu36I CC'TnA_GG
Cuts at: 0 869 3927
Size: 869 3058
BtgI C'CryG_G
Cuts at: 0 27 1388 1694 3646 3875 3927
Size: 27 1361 306 1952 229 52
Fragments arranged by size:
1952 1361 306 229 52 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1796 3927
Size: 1796 2131
BtsI GCAGTG_nn'
Cuts at: 0 2658 2670 3398 3927
Size: 2658 12 728 529
Fragments arranged by size:
2658 728 529 12
DraIII CAC_nnn'GTG
Cuts at: 0 1899 3927
Size: 1899 2028
EaeI y'GGCC_r
Cuts at: 0 429 677 832 971 1065 1503 1556 1795
Size: 429 248 155 139 94 438 53 239
Cuts at: 1795 2141 2226 2459 2580 3152 3459 3525 3927
Size: 346 85 233 121 572 307 66 402
Fragments arranged by size:
572 438 429 402 346 307 248 239
233 155 139 121 94 85 66 53
EagI C'GGCC_G
Cuts at: 0 429 2580 3927
Size: 429 2151 1347
Fragments arranged by size:
2151 1347 429
EarI CTCTTCn'nnn_
Cuts at: 0 1768 2805 3067 3915 3927
Size: 1768 1037 262 848 12
Fragments arranged by size:
1768 1037 848 262 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1648 3228 3927
Size: 1648 1580 699
Fragments arranged by size:
1648 1580 699
EcoICRI GAG'CTC
Cuts at: 0 3428 3741 3927
Size: 3428 313 186
Fragments arranged by size:
3428 313 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 757 868 877 910 1006 1387 1453
Size: 19 738 111 9 33 96 381 66
Cuts at: 1453 2163 2625 2868 2970 3091 3130 3414 3927
Size: 710 462 243 102 121 39 284 513
Fragments arranged by size:
738 710 513 462 381 284 243 121
111 102 96 66 39 33 19 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 973 1570 3927
Size: 889 84 597 2357
Fragments arranged by size:
2357 889 597 84
EcoO109I rG'GnC_Cy
Cuts at: 0 488 978 1812 1833 2258 2314 2401 2410
Size: 488 490 834 21 425 56 87 9
Cuts at: 2410 2601 3000 3133 3927
Size: 191 399 133 794
Fragments arranged by size:
834 794 490 488 425 399 191 133
87 56 21 9
EcoRV GAT'ATC
Cuts at: 0 3089 3927
Size: 3089 838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2385 2898 3927
Size: 2385 513 1029
Fragments arranged by size:
2385 1029 513
HaeII r_GCGC'y
Cuts at: 0 27 605 616 1176 1314 1432 2549 3777
Size: 27 578 11 560 138 118 1117 1228
Cuts at: 3777 3927
Size: 150
Fragments arranged by size:
1228 1117 578 560 150 138 118 27
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 1589 1621 2050 2082 3174 3192
Size: 239 32 1318 32 429 32 1092 18
Cuts at: 3192 3206 3224 3927
Size: 14 18 703
Fragments arranged by size:
1318 1092 703 429 239 32 32 32
18 18 14
HindIII A'AGCT_T
Cuts at: 0 3499 3927
Size: 3499 428
KasI G'GCGC_C
Cuts at: 0 23 601 1310 2545 3927
Size: 23 578 709 1235 1382
Fragments arranged by size:
1382 1235 709 578 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1971 3927
Size: 471 1500 1956
Fragments arranged by size:
1956 1500 471
MscI TGG'CCA
Cuts at: 0 679 973 1505 1558 1797 3154 3461 3927
Size: 679 294 532 53 239 1357 307 466
Fragments arranged by size:
1357 679 532 466 307 294 239 53
MslI CAynn'nnrTG
Cuts at: 0 1448 1552 2467 3127 3859 3927
Size: 1448 104 915 660 732 68
Fragments arranged by size:
1448 915 732 660 104 68
MspA1I CmG'CkG
Cuts at: 0 29 80 963 1383 1866 2458 2506 2645
Size: 29 51 883 420 483 592 48 139
Cuts at: 2645 2735 3451 3564 3648 3670 3927
Size: 90 716 113 84 22 257
Fragments arranged by size:
883 716 592 483 420 257 139 113
90 84 51 48 29 22
NaeI GCC'GGC
Cuts at: 0 2233 2580 3927
Size: 2233 347 1347
Fragments arranged by size:
2233 1347 347
NarI GG'CG_CC
Cuts at: 0 24 602 1311 2546 3927
Size: 24 578 709 1235 1381
Fragments arranged by size:
1381 1235 709 578 24
NcoI C'CATG_G
Cuts at: 0 1694 3927
Size: 1694 2233
NgoMIV G'CCGG_C
Cuts at: 0 2231 2578 3927
Size: 2231 347 1349
Fragments arranged by size:
2231 1349 347
NspI r_CATG'y
Cuts at: 0 1290 3927
Size: 1290 2637
PflMI CCAn_nnn'nTGG
Cuts at: 0 888 3927
Size: 888 3039
PfoI T'CCnGG_A
Cuts at: 0 2260 3927
Size: 2260 1667
PmlI CAC'GTG
Cuts at: 0 522 3927
Size: 522 3405
PpuMI rG'GwC_Cy
Cuts at: 0 488 978 1833 2258 2314 3000 3927
Size: 488 490 855 425 56 686 927
Fragments arranged by size:
927 855 686 490 488 425 56
PspOMI G'GGCC_C
Cuts at: 0 1377 2601 3132 3910 3927
Size: 1377 1224 531 778 17
Fragments arranged by size:
1377 1224 778 531 17
PspXI vC'TCGA_Gb
Cuts at: 0 2484 3927
Size: 2484 1443
PstI C_TGCA'G
Cuts at: 0 575 752 2409 3927
Size: 575 177 1657 1518
Fragments arranged by size:
1657 1518 575 177
PvuII CAG'CTG
Cuts at: 0 963 2506 3451 3670 3927
Size: 963 1543 945 219 257
Fragments arranged by size:
1543 963 945 257 219
SacI G_AGCT'C
Cuts at: 0 3430 3743 3927
Size: 3430 313 184
Fragments arranged by size:
3430 313 184
SacII CC_GC'GG
Cuts at: 0 30 3649 3927
Size: 30 3619 278
Fragments arranged by size:
3619 278 30
SanDI GG'GwC_CC
Cuts at: 0 2314 3927
Size: 2314 1613
SapI GCTCTTCn'nnn_
Cuts at: 0 3067 3927
Size: 3067 860
SbfI CC_TGCA'GG
Cuts at: 0 2409 3927
Size: 2409 1518
SexAI A'CCwGG_T
Cuts at: 0 1145 1843 3927
Size: 1145 698 2084
Fragments arranged by size:
2084 1145 698
SfcI C'TryA_G
Cuts at: 0 571 748 2121 2405 3927
Size: 571 177 1373 284 1522
Fragments arranged by size:
1522 1373 571 284 177
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2408 3927
Size: 2408 1519
SfoI GGC'GCC
Cuts at: 0 25 603 1312 2547 3927
Size: 25 578 709 1235 1380
Fragments arranged by size:
1380 1235 709 578 25
SmaI CCC'GGG
Cuts at: 0 328 473 3618 3927
Size: 328 145 3145 309
Fragments arranged by size:
3145 328 309 145
SmlI C'TyrA_G
Cuts at: 0 2484 3927
Size: 2484 1443
SrfI GCCC'GGGC
Cuts at: 0 473 3618 3927
Size: 473 3145 309
Fragments arranged by size:
3145 473 309
StuI AGG'CCT
Cuts at: 0 2568 3927
Size: 2568 1359
StyI C'CwwG_G
Cuts at: 0 173 542 921 974 1694 2563 2595 3155
Size: 173 369 379 53 720 869 32 560
Cuts at: 3155 3927
Size: 772
Fragments arranged by size:
869 772 720 560 379 369 173 53
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 138 309 1756 1906 2902 3025 3346 3426
Size: 138 171 1447 150 996 123 321 80
Cuts at: 3426 3927
Size: 501
Fragments arranged by size:
1447 996 501 321 171 150 138 123
80
TatI w'GTAC_w
Cuts at: 0 947 1406 1634 3927
Size: 947 459 228 2293
Fragments arranged by size:
2293 947 459 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 428 460 2166 2185 2198 2217
Size: 367 32 29 32 1706 19 13 19
Cuts at: 2217 3325 3357 3927
Size: 1108 32 570
Fragments arranged by size:
1706 1108 570 367 32 32 32 29
19 19 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 981 1685 3162 3927
Size: 555 426 704 1477 765
Fragments arranged by size:
1477 765 704 555 426
XhoI C'TCGA_G
Cuts at: 0 2484 3927
Size: 2484 1443
XmaI C'CCGG_G
Cuts at: 0 326 471 3616 3927
Size: 326 145 3145 311
Fragments arranged by size:
3145 326 311 145
ZraI GAC'GTC
Cuts at: 0 2925 3054 3927
Size: 2925 129 873
Fragments arranged by size:
2925 873 129
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
Acc65I AflII AhdI AscI AseI AsiSI AvrII BaeI
BciVI BfrBI BmtI BpuEI BsiWI BsmI BsrDI BsrGI
BssHII BstBI BstZ17I ClaI CspCI DraI DrdI EcoRI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI
ScaI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XmnI