Restriction map for
pFN21AB6529
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# NO CUT ENZYMES ( 98 enzymes) #
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AccI AclI AcsI AgeI Aor51HI
ApaI ApoI AscI AseI AsnI
AspI AvrII BamHI BbrPI BcgI
BciVI BclI BlnI BsaBI BsaI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI Bst1107I
BstAPI BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraIII Eco47III EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HindIII HpaI KspI MamI
MfeI MluI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 72 enzymes) #
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AatII 37 GACGTC GACGT^C
Acc65I 560 GGTACC G^GTACC
AccIII 11 TCCGGA T^CCGGA
AflII 412 CTTAAG C^TTAAG
AflIII 74 ACGTGT A^C[GA][CT]GT
Alw44I 439 GTGCAC G^TGCAC
AocI 940 CCTGAGG CC^T[ACGT]AGG
ApaLI 439 GTGCAC G^TGCAC
Asp700I 801 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 560 GGTACC G^GTACC
AvaI 571 CCCGAG C^[CT]CG[GA]G
AviII 290 TGCGCA TGC^GCA
BalI 152 TGGCCA TGG^CCA
BbeI 1073 GGCGCC GGCGC^C
BbsI 268 GTCTTC GAAGAC(2/6)
BbvCI 582 CCTCAGC CCTCAGC(-5/-2)
BfaI 871 CTAG C^TAG
BfrI 412 CTTAAG C^TTAAG
BglII 188 AGATCT A^GATCT
BlpI 165 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 165 GCTGAGC GC^T[ACGT]AGC
BpuAI 268 GTCTTC GAAGAC(2/6)
BsaWI 11 TCCGGA [AT]^CCGG[AT]
BseAI 11 TCCGGA T^CCGGA
BsgI 739 GTGCAG GTGCAG(16/14)
BsiEI 893 CGGCCG CG[GA][CT]^CG
BsmAI 949 GAGAC GTCTC(1/5)
BsmBI 949 GAGACG CGTCTC(1/5)
BsoBI 571 CCCGAG C^[CT]CG[GA]G
BspEI 11 TCCGGA T^CCGGA
BspMI 848 ACCTGC ACCTGC(4/8)
BsrDI 1053 CATTGC GCAATG(2/0)
BssHII 201 GCGCGC G^CGCGC
BssSI 988 CACGAG CACGAG(-5/-1)
BstEII 549 GGTGACC G^GT[ACGT]ACC
BstPI 549 GGTGACC G^GT[ACGT]ACC
BstXI 423 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 940 CCTGAGG CC^T[ACGT]AGG
CelII 165 GCTGAGC GC^T[ACGT]AGC
DrdI 69 GACCTACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 823 CCACGG C^C[GA][CT]GG
EagI 893 CGGCCG C^GGCCG
EarI 946 GAAGAG CTCTTC(1/4)
EclXI 893 CGGCCG C^GGCCG
Eco52I 893 CGGCCG C^GGCCG
Eco81I 940 CCTGAGG CC^T[ACGT]AGG
EcoO65I 549 GGTGACC G^GT[ACGT]ACC
FspI 290 TGCGCA TGC^GCA
HincII 40 GTCAAC GT[CT]^[GA]AC
HindII 40 GTCAAC GT[CT]^[GA]AC
KasI 1073 GGCGCC G^GCGCC
KpnI 560 GGTACC GGTAC^C
Ksp632I 946 GAAGAG CTCTTC(1/4)
MaeI 871 CTAG C^TAG
MluNI 152 TGGCCA TGG^CCA
MroI 11 TCCGGA T^CCGGA
MscI 152 TGGCCA TGG^CCA
MseI 413 TTAA T^TAA
MslI 351 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1073 GGCGCC GG^CGCC
PleI 1066 GACTC GAGTC(4/5)
PshAI 84 GACGCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 340 CTGCAG CTGCA^G
SfcI 340 CTGCAG C^T[GA][CT]AG
SfiI 894 GGCCGCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1073 GGCGCC GGC^GCC
SmlI 412 CTTAAG C^T[CT][GA]AG
StuI 408 AGGCCT AGG^CCT
TaqI 128 TCGA T^CGA
Tru9I 413 TTAA T^TAA
TthHB8I 128 TCGA T^CGA
XmnI 801 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AhdI 130 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
613 GACATCTGGTC
AspEI 130 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
613 GACATCTGGTC
AspHI 117 GTGCTC G[AT]GC[AT]^C
439 GTGCAC
BanII 256 GGGCTC G[GA]GC[CT]^C
566 GAGCCC
BglI 895 GCCGCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1076 GCCGGAGTGGC
BpmI 853 CTGGAG CTGGAG(16/14)
865 CTGGAG
BsaAI 73 TACGTG [CT]AC^GT[GA]
544 TACGTG
BseRI 282 CTCCTC GAGGAG(10/8)
370 GAGGAG
BsiHKAI 117 GTGCTC G[AT]GC[AT]^C
439 GTGCAC
Eam1105I 130 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
613 GACATCTGGTC
FauI 554 CCCGC CCCGC(4/6)
98 GCGGG
HaeII 884 AGCGCC [GA]GCGC^[CT]
1073 GGCGCC
HgaI 84 GACGC GACGC(5/10)
526 GACGC
MaeIII 550 GTGAC ^GT[ACGT]AC
712 GTGAC
NspI 233 ACATGC [GA]CATG^[CT]
519 ACATGC
PflMI 381 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
603 CCAGACAGTGG
PpuMI 446 GGGACCT [GA]G^G[AT]CC[CT]
935 GGGACCC
SexAI 311 ACCTGGT A^CC[AT]GGT
386 ACCTGGT
TfiI 495 GATTC G^A[AT]TC
976 GATTC
Tsp45I 550 GTGAC ^GT[GC]AC
712 GTGAC
Van91I 381 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
603 CCAGACAGTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaII BanI BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI XhoII