Restriction map for
pFN21AB8452
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BbrPI BcgI BciVI BfrI
BglII BlnI BsaBI BseAI BseRI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EarI
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
Ksp632I KspI MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 539 GGTACC G^GTACC
AhdI 592 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 539 GGTACC G^GTACC
AspEI 592 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 386 GGATCC G^GATCC
BbeI 106 GGCGCC GGCGC^C
BbsI 457 GAAGAC GAAGAC(2/6)
BbvCI 420 GCTGAGG CCTCAGC(-5/-2)
BclI 626 TGATCA T^GATCA
BfaI 373 CTAG C^TAG
BlpI 1014 GCTCAGC GC^T[ACGT]AGC
BpmI 832 CTGGAG CTGGAG(16/14)
Bpu1102I 1014 GCTCAGC GC^T[ACGT]AGC
BpuAI 457 GAAGAC GAAGAC(2/6)
BsaAI 523 TACGTG [CT]AC^GT[GA]
BsaWI 383 ACCGGA [AT]^CCGG[AT]
BsmBI 1060 GAGACG CGTCTC(1/5)
BspMI 86 ACCTGC ACCTGC(4/8)
BsrFI 145 ACCGGC [GA]^CCGG[CT]
BstAPI 720 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1014 GCTCAGC GC^T[ACGT]AGC
Cfr10I 145 ACCGGC [GA]^CCGG[CT]
Eam1105I 592 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1055 CCAAGG C^C[AT][AT]GG
FbaI 626 TGATCA T^GATCA
HincII 967 GTTGAC GT[CT]^[GA]AC
HindII 967 GTTGAC GT[CT]^[GA]AC
KasI 106 GGCGCC G^GCGCC
KpnI 539 GGTACC GGTAC^C
MaeI 373 CTAG C^TAG
MslI 684 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 106 GGCGCC GG^CGCC
NspI 239 ACATGC [GA]CATG^[CT]
SacI 3 GAGCTC GAGCT^C
SexAI 317 ACCTGGT A^CC[AT]GGT
SfoI 106 GGCGCC GGC^GCC
SgrAI 144 CACCGGCG C[GA]^CCGG[CT]G
SmlI 232 CTCAAG C^T[CT][GA]AG
StyI 1055 CCAAGG C^C[AT][AT]GG
TaqI 902 TCGA T^CGA
TthHB8I 902 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 406 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
721 CAGCGGCTG
AvaI 102 CTCGGG C^[CT]CG[GA]G
550 CCCGAG
BalI 158 TGGCCA TGG^CCA
491 TGGCCA
BsaI 289 GAGACC GGTCTC(1/5)
1050 GGTCTC
BsgI 910 CTGCAC GTGCAG(16/14)
718 GTGCAG
BsiEI 128 CGGCCG CG[GA][CT]^CG
138 CGGCCG
BsoBI 102 CTCGGG C^[CT]CG[GA]G
550 CCCGAG
BspHI 356 TCATGA T^CATGA
683 TCATGA
BssHII 69 GCGCGC G^CGCGC
207 GCGCGC
BssSI 247 CACGAG CACGAG(-5/-1)
397 CTCGTG
BstEII 45 GGTGACC G^GT[ACGT]ACC
528 GGTGACC
BstPI 45 GGTGACC G^GT[ACGT]ACC
528 GGTGACC
BstYI 386 GGATCC [GA]^GATC[CT]
473 AGATCC
EagI 128 CGGCCG C^GGCCG
138 CGGCCG
EclXI 128 CGGCCG C^GGCCG
138 CGGCCG
Eco52I 128 CGGCCG C^GGCCG
138 CGGCCG
EcoO65I 45 GGTGACC G^GT[ACGT]ACC
528 GGTGACC
HinfI 904 GAGTC G^A[ACGT]TC
952 GACTC
MflI 386 GGATCC [GA]^GATC[CT]
473 AGATCC
MluNI 158 TGGCCA TGG^CCA
491 TGGCCA
MscI 158 TGGCCA TGG^CCA
491 TGGCCA
PleI 952 GACTC GAGTC(4/5)
904 GAGTC
PpuMI 83 GGGACCT [GA]G^G[AT]CC[CT]
425 GGGACCT
PstI 88 CTGCAG CTGCA^G
727 CTGCAG
PvuII 670 CAGCTG CAG^CTG
1036 CAGCTG
RcaI 356 TCATGA T^CATGA
683 TCATGA
SfcI 88 CTGCAG C^T[GA][CT]AG
727 CTGCAG
Tsp509I 565 AATT ^AATT
770 AATT
XhoII 386 GGATCC [GA]^GATC[CT]
473 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI