(Circular) (Six-base) MAPSORT of: pFN21AB8452.seq Check: 9499 from: 1 to: 5776 pFN21AB8452 5776 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 2544 3097 2544 Size: 553 5223 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2456 2495 2699 3839 5223 5491 1632 Size: 806 18 39 204 1140 1384 268 1917 Fragments arranged by size: 1917 1384 1140 806 268 204 39 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 2602 4505 2602 Size: 1903 3873 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2416 2731 3519 5091 2416 Size: 315 788 1572 3101 Fragments arranged by size: 3101 1572 788 315 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2555 3101 1870 Size: 73 164 448 546 4545 Fragments arranged by size: 4545 546 448 164 73 BamHI G'GATC_C Cuts at: 2391 3106 3473 2391 Size: 715 367 4694 Fragments arranged by size: 4694 715 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2342 2544 3097 4552 Size: 325 217 627 324 231 202 553 1455 Cuts at: 4552 618 Size: 1842 Fragments arranged by size: 1842 1455 627 553 325 324 231 217 202 BanII G_rGCy'C Cuts at: 1391 2012 2108 2554 2937 3095 1391 Size: 621 96 446 383 158 4072 Fragments arranged by size: 4072 621 446 383 158 96 BbeI G_GCGC'C Cuts at: 2115 2115 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2469 928 Size: 971 570 4235 Fragments arranged by size: 4235 971 570 BbvCI CC'TCA_GC Cuts at: 2426 2426 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 2631 1548 Size: 1083 4693 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2151 2504 3037 Size: 107 122 71 631 605 479 353 533 Cuts at: 3037 4387 136 Size: 1350 1525 Fragments arranged by size: 1525 1350 631 605 533 479 353 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3020 3193 3020 Size: 173 5603 Bme1580I G_kGCm'C Cuts at: 1790 2345 3837 4993 1790 Size: 555 1492 1156 2573 Fragments arranged by size: 2573 1492 1156 555 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2532 3507 4465 Size: 416 53 83 399 24 1115 975 958 Cuts at: 4465 442 Size: 1753 Fragments arranged by size: 1753 1115 975 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2858 4436 1239 Size: 1619 1578 2579 Fragments arranged by size: 2579 1619 1578 Bpu10I CC'TnA_GC Cuts at: 2157 2426 2157 Size: 269 5507 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 3901 4766 5064 2222 Size: 1679 865 298 2934 Fragments arranged by size: 2934 1679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2288 3061 4439 882 Size: 617 789 773 1378 2219 Fragments arranged by size: 2219 1378 789 773 617 BsaAI yAC'GTr Cuts at: 493 1204 2530 3567 493 Size: 711 1326 1037 2702 Fragments arranged by size: 2702 1326 1037 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2705 3068 3583 Size: 53 83 186 936 579 593 363 515 Cuts at: 3583 3965 4600 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 593 579 515 382 363 186 83 53 BsaWI w'CCGG_w Cuts at: 2388 4209 4607 4881 5028 5439 2388 Size: 1821 398 274 147 411 2725 Fragments arranged by size: 2725 1821 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 3043 4979 5475 3043 Size: 1936 496 3344 Fragments arranged by size: 3344 1936 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2744 2900 1163 Size: 573 1008 156 4039 Fragments arranged by size: 4039 1008 573 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 2146 3226 3987 5015 1540 Size: 275 186 135 10 1080 761 1028 2301 Fragments arranged by size: 2301 1080 1028 761 275 186 135 10 BsiHKAI G_wGCw'C Cuts at: 2012 2132 3021 3837 3922 4993 2012 Size: 120 889 816 85 1071 2795 Fragments arranged by size: 2795 1071 889 816 120 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 3059 3059 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2108 2132 2345 2554 2937 3021 Size: 399 222 96 24 213 209 383 84 Cuts at: 3021 3095 3837 3922 4993 1391 Size: 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 383 222 213 209 96 85 84 74 24 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 2361 2688 3665 5395 2361 Size: 327 977 1730 2742 Fragments arranged by size: 2742 1730 977 327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 3131 844 Size: 1256 1031 3489 Fragments arranged by size: 3489 1256 1031 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 4420 4607 1700 Size: 110 340 2270 187 2869 Fragments arranged by size: 2869 2270 340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 2074 2212 1925 Size: 149 138 5489 Fragments arranged by size: 5489 149 138 BssSI C'ACGA_G Cuts at: 2252 2402 3836 4848 2252 Size: 150 1434 1012 3180 Fragments arranged by size: 3180 1434 1012 150 BstAPI GCAn_nnn'nTGC Cuts at: 794 2731 794 Size: 1937 3839 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 2050 2533 1184 Size: 866 483 4427 Fragments arranged by size: 4427 866 483 BstYI r'GATC_y Cuts at: 1972 2391 2478 3106 3473 3858 3875 5327 5413 Size: 419 87 628 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 628 419 385 367 346 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 2136 2607 513 Size: 552 1035 36 471 3682 Fragments arranged by size: 3682 1035 552 471 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2754 487 Size: 683 56 1528 3509 Fragments arranged by size: 3509 1528 683 56 BtsI GCAGTG_nn' Cuts at: 2035 3292 4086 4106 2035 Size: 1257 794 20 3705 Fragments arranged by size: 3705 1257 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2143 2163 Size: 54 1425 50 275 18 303 10 20 Cuts at: 2163 2496 3223 4112 5741 8 Size: 333 727 889 1629 43 Fragments arranged by size: 1629 1425 889 727 333 303 275 54 50 43 20 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2133 2143 3223 1537 Size: 275 321 10 1080 4090 Fragments arranged by size: 4090 1080 321 275 10 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2004 2639 4363 4737 4883 387 Size: 1064 138 415 635 1724 374 146 1280 Fragments arranged by size: 1724 1280 1064 635 415 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 2456 2495 2699 2858 3839 4436 Size: 393 806 18 39 204 159 981 597 Cuts at: 4436 5223 5491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 981 806 787 597 393 268 204 159 39 18 EcoO109I rG'GnC_Cy Cuts at: 2089 2431 2764 2089 Size: 342 333 5101 Fragments arranged by size: 5101 342 333 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2115 2157 2891 4923 1678 Size: 437 42 734 2032 2531 Fragments arranged by size: 2531 2032 734 437 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 2780 2812 4417 Size: 32 135 32 1016 32 160 32 1605 Cuts at: 4417 4449 4491 4523 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1605 1016 160 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2974 3120 3366 669 Size: 988 281 1036 146 246 3079 Fragments arranged by size: 3079 1036 988 281 246 146 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2111 2111 Size: 5776 KpnI G_GTAC'C Cuts at: 2548 3101 2548 Size: 553 5223 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 2498 10 Size: 54 1425 343 333 333 3288 Fragments arranged by size: 3288 1425 343 333 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 3734 4093 4252 518 Size: 648 1527 1041 359 159 2042 Fragments arranged by size: 2042 1527 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2677 2728 2869 3043 3192 3869 5017 5262 Size: 1071 51 141 174 149 677 1148 245 Cuts at: 5262 1606 Size: 2120 Fragments arranged by size: 2120 1148 1071 677 245 174 149 141 51 NarI GG'CG_CC Cuts at: 2112 2112 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2248 3134 4679 1132 Size: 97 1019 886 1545 2229 Fragments arranged by size: 2229 1545 1019 886 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2481 2835 2481 Size: 354 5422 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2431 2089 Size: 342 5434 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 2097 2736 3128 830 Size: 1267 639 392 3478 Fragments arranged by size: 3478 1267 639 392 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2677 3043 2677 Size: 366 5410 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SexAI A'CCwGG_T Cuts at: 2322 2322 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2093 2732 3124 4259 4940 5131 826 Size: 219 1048 639 392 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 681 639 392 219 191 SfoI GGC'GCC Cuts at: 2113 2113 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 2150 1700 Size: 450 5326 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 3880 4781 5043 820 Size: 197 926 294 1643 901 262 1553 Fragments arranged by size: 1643 1553 926 901 294 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 3060 3215 513 Size: 552 1995 155 3074 Fragments arranged by size: 3074 1995 552 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI