Restriction map for
pFN21AB8452
(Linear) (Six-base) MAPSORT of: KIBB8452.seq Check: 1023 from: 1 to: 1071
KIBB8452.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
Acc65I G'GTAC_C
Cuts at: 0 539 1071
Size: 539 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 451 490 694 1071
Size: 433 18 39 204 377
Fragments arranged by size:
433 377 204 39 18
AhdI GACnn_n'nnGTC
Cuts at: 0 597 1071
Size: 597 474
AlwNI CAG_nnn'CTG
Cuts at: 0 411 726 1071
Size: 411 315 345
Fragments arranged by size:
411 345 315
AvaI C'yCGr_G
Cuts at: 0 102 550 1071
Size: 102 448 521
Fragments arranged by size:
521 448 102
BamHI G'GATC_C
Cuts at: 0 386 1071
Size: 386 685
BanI G'GyrC_C
Cuts at: 0 106 337 539 1071
Size: 106 231 202 532
Fragments arranged by size:
532 231 202 106
BanII G_rGCy'C
Cuts at: 0 7 103 549 932 1071
Size: 7 96 446 383 139
Fragments arranged by size:
446 383 139 96 7
BbeI G_GCGC'C
Cuts at: 0 110 1071
Size: 110 961
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 1071
Size: 464 607
BbvCI CC'TCA_GC
Cuts at: 0 421 1071
Size: 421 650
BclI T'GATC_A
Cuts at: 0 626 1071
Size: 626 445
BglI GCCn_nnn'nGGC
Cuts at: 0 146 499 1032 1071
Size: 146 353 533 39
Fragments arranged by size:
533 353 146 39
BlpI GC'TnA_GC
Cuts at: 0 1015 1071
Size: 1015 56
Bme1580I G_kGCm'C
Cuts at: 0 340 1071
Size: 340 731
BmrI ACTGGGnnnn_n'
Cuts at: 0 527 1071
Size: 527 544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1071
Size: 853 218
Bpu10I CC'TnA_GC
Cuts at: 0 152 421 1071
Size: 152 269 650
Fragments arranged by size:
650 269 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1071
Size: 217 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 283 1056 1071
Size: 283 773 15
Fragments arranged by size:
773 283 15
BsaAI yAC'GTr
Cuts at: 0 525 1071
Size: 525 546
BsaHI Gr'CG_yC
Cuts at: 0 107 700 1063 1071
Size: 107 593 363 8
Fragments arranged by size:
593 363 107 8
BsaWI w'CCGG_w
Cuts at: 0 383 1071
Size: 383 688
BseYI C'CCAG_C
Cuts at: 0 1038 1071
Size: 1038 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 895 1071
Size: 739 156 176
Fragments arranged by size:
739 176 156
BsiEI CG_ry'CG
Cuts at: 0 131 141 1071
Size: 131 10 930
Fragments arranged by size:
930 131 10
BsiHKAI G_wGCw'C
Cuts at: 0 7 127 1016 1071
Size: 7 120 889 55
Fragments arranged by size:
889 120 55 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1054 1071
Size: 1054 17
Bsp1286I G_dGCh'C
Cuts at: 0 7 103 127 340 549 932 1016 1071
Size: 7 96 24 213 209 383 84 55
Fragments arranged by size:
383 213 209 96 84 55 24 7
BspHI T'CATG_A
Cuts at: 0 356 683 1071
Size: 356 327 388
Fragments arranged by size:
388 356 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 1071
Size: 95 976
BsrFI r'CCGG_y
Cuts at: 0 145 1071
Size: 145 926
BssHII G'CGCG_C
Cuts at: 0 69 207 1071
Size: 69 138 864
Fragments arranged by size:
864 138 69
BssSI C'ACGA_G
Cuts at: 0 247 397 1071
Size: 247 150 674
Fragments arranged by size:
674 247 150
BstAPI GCAn_nnn'nTGC
Cuts at: 0 726 1071
Size: 726 345
BstEII G'GTnAC_C
Cuts at: 0 45 528 1071
Size: 45 483 543
Fragments arranged by size:
543 483 45
BstYI r'GATC_y
Cuts at: 0 386 473 1071
Size: 386 87 598
Fragments arranged by size:
598 386 87
BtgI C'CryG_G
Cuts at: 0 95 131 602 1071
Size: 95 36 471 469
Fragments arranged by size:
471 469 95 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 749 1071
Size: 749 322
BtsI GCAGTG_nn'
Cuts at: 0 30 1071
Size: 30 1041
EaeI y'GGCC_r
Cuts at: 0 128 138 158 491 1071
Size: 128 10 20 333 580
Fragments arranged by size:
580 333 128 20 10
EagI C'GGCC_G
Cuts at: 0 128 138 1071
Size: 128 10 933
Fragments arranged by size:
933 128 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 634 1071
Size: 634 437
EcoICRI GAG'CTC
Cuts at: 0 5 1071
Size: 5 1066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 451 490 694 853 1071
Size: 433 18 39 204 159 218
Fragments arranged by size:
433 218 204 159 39 18
EcoO109I rG'GnC_Cy
Cuts at: 0 84 426 759 1071
Size: 84 342 333 312
Fragments arranged by size:
342 333 312 84
HaeII r_GCGC'y
Cuts at: 0 110 152 886 1071
Size: 110 42 734 185
Fragments arranged by size:
734 185 110 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 775 807 1071
Size: 583 32 160 32 264
Fragments arranged by size:
583 264 160 32 32
HincII GTy'rAC
Cuts at: 0 969 1071
Size: 969 102
KasI G'GCGC_C
Cuts at: 0 106 1071
Size: 106 965
KpnI G_GTAC'C
Cuts at: 0 543 1071
Size: 543 528
MscI TGG'CCA
Cuts at: 0 160 493 1071
Size: 160 333 578
Fragments arranged by size:
578 333 160
MslI CAynn'nnrTG
Cuts at: 0 688 1071
Size: 688 383
MspA1I CmG'CkG
Cuts at: 0 672 723 864 1038 1071
Size: 672 51 141 174 33
Fragments arranged by size:
672 174 141 51 33
NarI GG'CG_CC
Cuts at: 0 107 1071
Size: 107 964
NspI r_CATG'y
Cuts at: 0 243 1071
Size: 243 828
PfoI T'CCnGG_A
Cuts at: 0 476 830 1071
Size: 476 354 241
Fragments arranged by size:
476 354 241
PpuMI rG'GwC_Cy
Cuts at: 0 84 426 1071
Size: 84 342 645
Fragments arranged by size:
645 342 84
PstI C_TGCA'G
Cuts at: 0 92 731 1071
Size: 92 639 340
Fragments arranged by size:
639 340 92
PvuII CAG'CTG
Cuts at: 0 672 1038 1071
Size: 672 366 33
Fragments arranged by size:
672 366 33
SacI G_AGCT'C
Cuts at: 0 7 1071
Size: 7 1064
SexAI A'CCwGG_T
Cuts at: 0 317 1071
Size: 317 754
SfcI C'TryA_G
Cuts at: 0 88 727 1071
Size: 88 639 344
Fragments arranged by size:
639 344 88
SfoI GGC'GCC
Cuts at: 0 108 1071
Size: 108 963
SgrAI Cr'CCGG_yG
Cuts at: 0 145 1071
Size: 145 926
SmlI C'TyrA_G
Cuts at: 0 232 1071
Size: 232 839
StyI C'CwwG_G
Cuts at: 0 1055 1071
Size: 1055 16
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI
BssHII BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI
EaeI EagI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII KasI KpnI MscI MslI MspA1I NarI NspI
PfoI PpuMI PstI PvuII SacI SexAI SfcI SfoI
SgrAI SmlI StyI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BmgBI
BmtI BplI BsaBI BsaXI BseRI BsiWI BsmI BspEI
BsrBI BsrDI BsrGI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI