################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI BbeI BbrPI BciVI BfrI BglI BglII BsaHI BseAI BsiWI BsmBI BspEI BsrBI BssHII Bst1107I BstAPI BstZ17I CpoI DraIII DrdI Eco47III EcoRV FseI HaeII Hin1I HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SacII SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AocI 2785 CCTCAGG CC^T[ACGT]AGG AviII 2856 TGCGCA TGC^GCA AvrII 1405 CCTAGG C^CTAGG BamHI 2123 GGATCC G^GATCC BbsI 205 GAAGAC GAAGAC(2/6) BclI 465 TGATCA T^GATCA BlnI 1405 CCTAGG C^CTAGG BlpI 586 GCTAAGC GC^T[ACGT]AGC Bpu1102I 586 GCTAAGC GC^T[ACGT]AGC BpuAI 205 GAAGAC GAAGAC(2/6) BsaAI 1670 CACGTA [CT]AC^GT[GA] BspHI 1020 TCATGA T^CATGA BsrFI 2940 GCCGGT [GA]^CCGG[CT] BssSI 4394 CTCGTG CACGAG(-5/-1) BstEII 3864 GGTGACC G^GT[ACGT]ACC BstPI 3864 GGTGACC G^GT[ACGT]ACC BstXI 4212 CCATGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2785 CCTCAGG CC^T[ACGT]AGG CelII 586 GCTAAGC GC^T[ACGT]AGC Cfr10I 2940 GCCGGT [GA]^CCGG[CT] EagI 4424 CGGCCG C^GGCCG EclXI 4424 CGGCCG C^GGCCG Eco52I 4424 CGGCCG C^GGCCG Eco81I 2785 CCTCAGG CC^T[ACGT]AGG EcoO65I 3864 GGTGACC G^GT[ACGT]ACC EcoRI 2491 GAATTC G^AATTC FbaI 465 TGATCA T^GATCA FspI 2856 TGCGCA TGC^GCA MfeI 3302 CAATTG C^AATTG MunI 3302 CAATTG C^AATTG PvuI 1692 CGATCG CGAT^CG RcaI 1020 TCATGA T^CATGA SspI 4026 AATATT AAT^ATT StuI 4477 AGGCCT AGG^CCT XbaI 201 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AflIII 3029 ACATGT A^C[GA][CT]GT 4161 ACATGT AlwNI 596 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 2962 CAGCAGCTG AseI 883 ATTAAT AT^TAAT 4275 ATTAAT AsnI 883 ATTAAT AT^TAAT 4275 ATTAAT AspI 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1556 GACATTGTC AvaI 112 CCCGAG C^[CT]CG[GA]G 130 CCCGAG BcgI 2715 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3929 GCATCAGCGTCG BsaBI 541 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2692 GATGCCTATC BsaWI 105 ACCGGA [AT]^CCGG[AT] 1413 ACCGGA BsiEI 1692 CGATCG CG[GA][CT]^CG 4424 CGGCCG BsoBI 112 CCCGAG C^[CT]CG[GA]G 130 CCCGAG BspDI 2689 ATCGAT AT^CGAT 3548 ATCGAT BspLU11I 3029 ACATGT A^CATGT 4161 ACATGT BstBI 1834 TTCGAA TT^CGAA 4030 TTCGAA CfoI 19 GCGC GCG^C 2857 GCGC ClaI 2689 ATCGAT AT^CGAT 3548 ATCGAT DraI 666 TTTAAA TTT^AAA 2163 TTTAAA EcoNI 1658 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3406 CCTGTTACAGG EcoT22I 2978 ATGCAT ATGCA^T 3372 ATGCAT FauI 34 CCCGC CCCGC(4/6) 1458 GCGGG HgaI 2062 GACGC GACGC(5/10) 3935 GCGTC HhaI 19 GCGC GCG^C 2857 GCGC HinP1I 19 GCGC G^CGC 2857 GCGC MamI 541 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2692 GATGCCTATC NsiI 2978 ATGCAT ATGCA^T 3372 ATGCAT NspV 1834 TTCGAA TT^CGAA 4030 TTCGAA PflFI 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1556 GACATTGTC PshBI 883 ATTAAT AT^TAAT 4275 ATTAAT SacI 2407 GAGCTC GAGCT^C 4075 GAGCTC SalI 907 GTCGAC G^TCGAC 3937 GTCGAC SapI 1117 GCTCTTC GCTCTTC(1/4) 2409 GCTCTTC SfuI 1834 TTCGAA TT^CGAA 4030 TTCGAA SpeI 2335 ACTAGT A^CTAGT 2838 ACTAGT SphI 813 GCATGC GCATG^C 2816 GCATGC Tth111I 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1556 GACATTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AhdI AluI AlwI ApoI Asp700I Asp718I AspEI AspHI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI