(Circular) (Six-base) MAPSORT of: pFN21AA0213.seq Check: 7014 from: 1 to: 9379 pFN21AA0213 9379 bp With 182 enzymes: SgfI * March 28, 2012 12:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4816 5939 4816 Size: 1123 8256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7189 278 Size: 53 83 186 936 5653 2468 Fragments arranged by size: 5653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2913 3559 3594 5943 6722 1937 Size: 976 646 35 2349 779 4594 Fragments arranged by size: 4594 2349 976 779 646 35 Acc65I G'GTAC_C Cuts at: 2249 3415 5464 5778 6700 2249 Size: 1166 2049 314 922 4928 Fragments arranged by size: 4928 2049 1166 922 314 AclI AA'CG_TT Cuts at: 1563 7506 7879 1563 Size: 5943 373 3063 Fragments arranged by size: 5943 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2949 3110 3626 3836 3917 4001 4427 4994 Size: 1317 161 516 210 81 84 426 567 Cuts at: 4994 6143 6408 6591 7442 8826 9094 1632 Size: 1149 265 183 851 1384 268 1917 Fragments arranged by size: 1917 1384 1317 1149 851 567 516 426 268 265 210 183 161 84 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9182 AflIII A'CryG_T Cuts at: 1203 5034 6166 8278 9255 1203 Size: 3831 1132 2112 977 1327 Fragments arranged by size: 3831 2112 1327 1132 977 AgeI A'CCGG_T Cuts at: 8210 8210 Size: 9379 AhdI GACnn_n'nnGTC Cuts at: 2655 3457 4938 8108 2655 Size: 802 1481 3170 3926 Fragments arranged by size: 3926 3170 1481 802 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3936 1184 Size: 2752 6627 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3834 3866 5021 5053 3834 Size: 32 1155 32 8160 Fragments arranged by size: 8160 1155 32 32 AlwNI CAG_nnn'CTG Cuts at: 2606 4972 7122 8694 2606 Size: 2366 2150 1572 3291 Fragments arranged by size: 3291 2366 2150 1572 ApaLI G'TGCA_C Cuts at: 7436 8592 7436 Size: 1156 8223 ApoI r'AATT_y Cuts at: 1493 3256 3719 4195 4304 4331 4496 4566 5048 Size: 1763 463 476 109 27 165 70 482 Cuts at: 5048 6688 6914 7066 9175 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1763 1697 1640 482 476 463 226 165 152 109 70 27 AseI AT'TA_AT Cuts at: 160 1032 2889 6281 7933 160 Size: 872 1857 3392 1652 1606 Fragments arranged by size: 3392 1857 1652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9379 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2135 6704 1870 Size: 73 174 18 4569 4545 Fragments arranged by size: 4569 4545 174 73 18 AvrII C'CTAG_G Cuts at: 3410 3410 Size: 9379 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2952 2985 2952 Size: 33 9346 BamHI G'GATC_C Cuts at: 4128 6709 7076 4128 Size: 2581 367 6431 Fragments arranged by size: 6431 2581 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 3415 4757 5464 5778 Size: 325 217 627 462 1166 1342 707 314 Cuts at: 5778 6251 6382 6700 8155 618 Size: 473 131 318 1455 1842 Fragments arranged by size: 1842 1455 1342 1166 707 627 473 462 325 318 314 217 131 BanII G_rGCy'C Cuts at: 1391 4405 4416 4735 6084 6698 1391 Size: 3014 11 319 1349 614 4072 Fragments arranged by size: 4072 3014 1349 614 319 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2217 928 Size: 971 318 8090 Fragments arranged by size: 8090 971 318 BbvCI CC'TCA_GC Cuts at: 2570 4614 5058 2570 Size: 2044 444 6891 Fragments arranged by size: 6891 2044 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4709 4743 5923 5957 7570 7604 1903 Size: 34 2772 34 1180 34 1613 34 3678 Fragments arranged by size: 3678 2772 1613 1180 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7271 8481 7271 Size: 1210 8169 BclI T'GATC_A Cuts at: 1548 2470 1548 Size: 922 8457 BfrBI ATG'CAT Cuts at: 4985 5379 4985 Size: 394 8985 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7990 136 Size: 107 122 71 631 605 6318 1525 Fragments arranged by size: 6318 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9374 9374 Size: 9379 BlpI GC'TnA_GC Cuts at: 2592 6796 2592 Size: 4204 5175 Bme1580I G_kGCm'C Cuts at: 1790 2168 2613 3239 3676 4760 7440 8596 1790 Size: 378 445 626 437 1084 2680 1156 2573 Fragments arranged by size: 2680 2573 1156 1084 626 445 437 378 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4508 6299 7110 Size: 416 53 83 399 24 3091 1791 811 Cuts at: 7110 8068 442 Size: 958 1753 Fragments arranged by size: 3091 1791 1753 958 811 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3556 3945 4055 5499 6460 8039 1239 Size: 2317 389 110 1444 961 1579 2579 Fragments arranged by size: 2579 2317 1579 1444 961 389 110 Bpu10I CC'TnA_GC Cuts at: 2570 4614 5058 2570 Size: 2044 444 6891 Fragments arranged by size: 6891 2044 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 3961 4137 5268 5371 5590 5881 6620 7504 Size: 455 176 1131 103 219 291 739 884 Cuts at: 7504 8369 8667 3506 Size: 865 298 4218 Fragments arranged by size: 4218 1131 884 865 739 455 298 291 219 176 103 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 3055 3910 4515 8042 882 Size: 617 615 941 855 605 3527 2219 Fragments arranged by size: 3527 2219 941 855 617 615 605 BsaAI yAC'GTr Cuts at: 493 1204 3677 7170 493 Size: 711 2473 3493 2702 Fragments arranged by size: 3493 2702 2473 711 BsaBI GATnn'nnATC Cuts at: 2550 4701 9373 2550 Size: 2151 4672 2556 Fragments arranged by size: 4672 2556 2151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7186 7568 8203 275 Size: 53 83 186 936 5653 382 635 1451 Fragments arranged by size: 5653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2110 3418 7812 8210 8484 8631 9042 2110 Size: 1308 4394 398 274 147 411 2447 Fragments arranged by size: 4394 2447 1308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9154 Fragments arranged by size: 9154 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 2087 3797 3824 4245 4631 5633 5661 Size: 839 62 1710 27 421 386 1002 28 Cuts at: 5661 5806 6629 1186 Size: 145 823 3936 Fragments arranged by size: 3936 1710 1002 839 823 421 386 145 62 28 27 BseYI C'CCAG_C Cuts at: 2261 3519 4752 6151 8582 9078 2261 Size: 1258 1233 1399 2431 496 2562 Fragments arranged by size: 2562 2431 1399 1258 1233 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2616 3194 3773 1163 Size: 573 880 578 579 6769 Fragments arranged by size: 6769 880 579 578 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 6432 6829 7590 8618 1540 Size: 275 186 1699 2732 397 761 1028 2301 Fragments arranged by size: 2732 2301 1699 1028 761 397 275 186 BsiHKAI G_wGCw'C Cuts at: 2495 3529 3542 4416 6084 6504 7440 7525 8596 Size: 1034 13 874 1668 420 936 85 1071 Cuts at: 8596 2495 Size: 3278 Fragments arranged by size: 3278 1668 1071 1034 936 874 420 85 13 BsmI GAATG_Cn' Cuts at: 2362 4278 5381 6890 6983 2362 Size: 1916 1103 1509 93 4758 Fragments arranged by size: 4758 1916 1509 1103 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2495 2613 3239 3529 3542 3676 Size: 399 378 327 118 626 290 13 134 Cuts at: 3676 4405 4416 4735 4760 6084 6504 6698 7440 Size: 729 11 319 25 1324 420 194 742 Cuts at: 7440 7525 8596 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1324 1071 742 729 626 420 399 378 327 319 290 194 134 118 85 25 13 11 BspEI T'CCGG_A Cuts at: 9042 9042 Size: 9379 BspHI T'CATG_A Cuts at: 3025 7268 8998 3025 Size: 4243 1730 3406 Fragments arranged by size: 4243 3406 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2638 3052 3853 3928 4816 5323 5939 6734 Size: 1794 414 801 75 888 507 616 795 Cuts at: 6734 844 Size: 3489 Fragments arranged by size: 3489 1794 888 801 795 616 507 414 75 BsrBI CCG'CTC Cuts at: 1394 1823 7266 1394 Size: 429 5443 3507 Fragments arranged by size: 5443 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2593 3113 4263 5017 5115 6198 7874 Size: 1183 1345 520 1150 754 98 1083 1676 Cuts at: 7874 8048 65 Size: 174 1396 Fragments arranged by size: 1676 1396 1345 1183 1150 1083 754 520 174 98 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 8023 8210 1700 Size: 110 3135 3078 187 2869 Fragments arranged by size: 3135 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2485 5925 6099 96 Size: 2389 3440 174 3376 Fragments arranged by size: 3440 3376 2389 174 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9379 BssSI C'ACGA_G Cuts at: 6399 7439 8451 6399 Size: 1040 1012 7327 Fragments arranged by size: 7327 1040 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9379 BstBI TT'CG_AA Cuts at: 3840 6036 8183 3840 Size: 2196 2147 5036 Fragments arranged by size: 5036 2196 2147 BstEII G'GTnAC_C Cuts at: 1184 5869 1184 Size: 4685 4694 BstXI CCAn_nnnn'nTGG Cuts at: 6224 6224 Size: 9379 BstYI r'GATC_y Cuts at: 1972 2439 2519 2551 3727 4081 4128 6709 7076 Size: 467 80 32 1176 354 47 2581 367 Cuts at: 7076 7461 7478 8930 9016 9028 9374 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2581 1977 1452 1176 467 385 367 354 346 86 80 47 32 17 12 Bsu36I CC'TnA_GG Cuts at: 4791 4791 Size: 9379 BtgI C'CryG_G Cuts at: 513 1065 2067 3822 5613 6418 513 Size: 552 1002 1755 1791 805 3474 Fragments arranged by size: 3474 1791 1755 1002 805 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4143 4936 6135 487 Size: 683 56 2917 793 1199 3731 Fragments arranged by size: 3731 2917 1199 793 683 56 BtsI GCAGTG_nn' Cuts at: 2984 3799 4363 6592 6895 7689 7709 2984 Size: 815 564 2229 303 794 20 4654 Fragments arranged by size: 4654 2229 815 794 564 303 20 ClaI AT'CG_AT Cuts at: 1554 4695 5554 1554 Size: 3141 859 5379 Fragments arranged by size: 5379 3141 859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9344 DraI TTT'AAA Cuts at: 2673 4170 6683 2673 Size: 1497 2513 5369 Fragments arranged by size: 5369 2513 1497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8386 809 Size: 507 7070 1802 Fragments arranged by size: 7070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3275 5383 5961 Size: 54 1425 50 275 18 1445 2108 578 Cuts at: 5961 6429 6826 7715 9344 8 Size: 468 397 889 1629 43 Fragments arranged by size: 2108 1629 1445 1425 889 578 468 397 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6429 6826 1537 Size: 275 4617 397 4090 Fragments arranged by size: 4617 4090 397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3129 4071 4421 4616 6132 7309 1353 Size: 1776 942 350 195 1516 1177 3423 Fragments arranged by size: 3423 1776 1516 1177 942 350 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7966 8340 8486 387 Size: 1064 138 6377 374 146 1280 Fragments arranged by size: 6377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4414 6082 4414 Size: 1668 7711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2949 3110 3556 3626 3836 3917 3945 Size: 393 1317 161 446 70 210 81 28 Cuts at: 3945 4001 4055 4427 4994 5499 6143 6408 6460 Size: 56 54 372 567 505 644 265 52 Cuts at: 6460 6591 7442 8039 8826 9094 1239 Size: 131 851 597 787 268 1524 Fragments arranged by size: 1524 1317 851 787 644 597 567 505 446 393 372 268 265 210 161 131 81 70 56 54 52 28 EcoNI CCTnn'n_nnAGG Cuts at: 3667 5415 3667 Size: 1748 7631 EcoO109I rG'GnC_Cy Cuts at: 3976 4477 5589 5729 3976 Size: 501 1112 140 7626 Fragments arranged by size: 7626 1112 501 140 EcoRI G'AATT_C Cuts at: 4496 6688 7066 4496 Size: 2192 378 6809 Fragments arranged by size: 6809 2192 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4432 5890 4432 Size: 1458 7921 FspI TGC'GCA Cuts at: 4863 7885 4863 Size: 3022 6357 HaeII r_GCGC'y Cuts at: 1678 8526 1678 Size: 6848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2641 2673 3443 Size: 32 135 32 539 32 498 32 770 Cuts at: 3443 3458 3475 3490 4919 4924 4951 4956 8020 Size: 15 17 15 1429 5 27 5 3064 Cuts at: 8020 8052 8094 8126 1373 Size: 32 42 32 2626 Fragments arranged by size: 3064 2626 1429 770 539 498 135 42 32 32 32 32 32 32 27 17 15 15 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2292 2914 4807 5944 6723 6969 Size: 988 281 354 622 1893 1137 779 246 Cuts at: 6969 669 Size: 3079 Fragments arranged by size: 3079 1893 1137 988 779 622 354 281 246 HindIII A'AGCT_T Cuts at: 748 2594 2705 3482 4250 4884 4959 5206 748 Size: 1846 111 777 768 634 75 247 4921 Fragments arranged by size: 4921 1846 777 768 634 247 111 75 HpaI GTT'AAC Cuts at: 6969 6969 Size: 9379 KpnI G_GTAC'C Cuts at: 2253 3419 5468 5782 6704 2253 Size: 1166 2049 314 922 4928 Fragments arranged by size: 4928 2049 1166 922 314 MfeI C'AATT_G Cuts at: 5307 6978 5307 Size: 1671 7708 MluI A'CGCG_T Cuts at: 9255 9255 Size: 9379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3061 3345 4389 8493 8677 1305 Size: 1756 284 1044 4104 184 2007 Fragments arranged by size: 4104 2007 1756 1044 284 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3277 5385 5963 10 Size: 54 1425 343 1445 2108 578 3426 Fragments arranged by size: 3426 2108 1445 1425 578 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4826 6107 6222 6423 6464 6495 7337 Size: 648 3660 1281 115 201 41 31 842 Cuts at: 7337 7696 7855 518 Size: 359 159 2042 Fragments arranged by size: 3660 2042 1281 842 648 359 201 159 115 41 31 MspA1I CmG'CkG Cuts at: 1606 2018 3955 4652 4752 4972 6795 7472 8620 Size: 412 1937 697 100 220 1823 677 1148 Cuts at: 8620 8865 1606 Size: 245 2120 Fragments arranged by size: 2120 1937 1823 1148 697 677 412 245 220 100 NcoI C'CATG_G Cuts at: 513 1065 2067 3822 6418 513 Size: 552 1002 1755 2596 3474 Fragments arranged by size: 3474 2596 1755 1002 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9379 NotI GC'GGCC_GC Cuts at: 6826 6826 Size: 9379 NsiI A_TGCA'T Cuts at: 4987 5381 4987 Size: 394 8985 NspI r_CATG'y Cuts at: 1132 1229 2290 2822 4825 5038 6170 6737 8282 Size: 97 1061 532 2003 213 1132 567 1545 Cuts at: 8282 1132 Size: 2229 Fragments arranged by size: 2229 2003 1545 1132 1061 567 532 213 97 PasI CC'CwG_GG Cuts at: 1348 6361 1348 Size: 5013 4366 PciI A'CATG_T Cuts at: 5034 6166 8278 5034 Size: 1132 2112 6135 Fragments arranged by size: 6135 2112 1132 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9379 PfoI T'CCnGG_A Cuts at: 2992 3162 4527 2992 Size: 170 1365 7844 Fragments arranged by size: 7844 1365 170 PmeI GTTT'AAAC Cuts at: 6683 6683 Size: 9379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3921 3953 7448 7480 8961 8993 3921 Size: 32 3495 32 1481 32 4307 Fragments arranged by size: 4307 3495 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3976 4477 5589 5729 3976 Size: 501 1112 140 7626 Fragments arranged by size: 7626 1112 501 140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9379 PsiI TTA'TAA Cuts at: 6949 6949 Size: 9379 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3998 4030 3998 Size: 32 9347 PstI C_TGCA'G Cuts at: 830 4026 4657 6019 6731 830 Size: 3196 631 1362 712 3478 Fragments arranged by size: 3478 3196 1362 712 631 PvuI CG_AT'CG Cuts at: 2001 3700 2001 Size: 1699 7680 PvuII CAG'CTG Cuts at: 3955 4652 4752 4972 3955 Size: 697 100 220 8362 Fragments arranged by size: 8362 697 220 100 SacI G_AGCT'C Cuts at: 4416 6084 4416 Size: 1668 7711 SalI G'TCGA_C Cuts at: 1936 2912 5942 6721 1936 Size: 976 3030 779 4594 Fragments arranged by size: 4594 3030 976 779 SapI GCTCTTCn'nnn_ Cuts at: 3129 4421 3129 Size: 1292 8087 SbfI CC_TGCA'GG Cuts at: 6731 6731 Size: 9379 ScaI AGT'ACT Cuts at: 4007 5096 6116 7627 9339 4007 Size: 1089 1020 1511 1712 4047 Fragments arranged by size: 4047 1712 1511 1089 1020 SfcI C'TryA_G Cuts at: 826 1045 2581 2619 4022 4139 4287 4653 6015 Size: 219 1536 38 1403 117 148 366 1362 Cuts at: 6015 6727 7862 8543 8734 826 Size: 712 1135 681 191 1471 Fragments arranged by size: 1536 1471 1403 1362 1135 712 681 366 219 191 148 117 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9379 SmaI CCC'GGG Cuts at: 1872 6706 1872 Size: 4834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3485 3940 4152 5283 5350 5605 Size: 197 926 1542 455 212 1131 67 255 Cuts at: 5605 5860 6599 7483 8384 8646 820 Size: 255 739 884 901 262 1553 Fragments arranged by size: 1553 1542 1131 926 901 884 739 455 262 255 255 212 197 67 SnaBI TAC'GTA Cuts at: 493 7170 493 Size: 6677 2702 SpeI A'CTAG_T Cuts at: 152 4340 4843 152 Size: 4188 503 4688 Fragments arranged by size: 4688 4188 503 SphI G_CATG'C Cuts at: 1132 2822 4825 6737 1132 Size: 1690 2003 1912 3774 Fragments arranged by size: 3774 2003 1912 1690 SspI AAT'ATT Cuts at: 5 52 6033 7303 5 Size: 47 5981 1270 2081 Fragments arranged by size: 5981 2081 1270 47 StuI AGG'CCT Cuts at: 1517 6484 1517 Size: 4967 4412 StyI C'CwwG_G Cuts at: 513 1065 2067 2575 3410 3822 6418 6485 6818 Size: 552 1002 508 835 412 2596 67 333 Cuts at: 6818 513 Size: 3074 Fragments arranged by size: 3074 2596 1002 835 552 508 412 333 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2322 2504 7406 7423 7576 7761 Size: 1255 558 184 182 4902 17 153 185 Cuts at: 7761 8206 325 Size: 445 1498 Fragments arranged by size: 4902 1498 1255 558 445 185 184 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2485 3090 4005 Size: 274 80 33 51 1443 508 605 915 Cuts at: 4005 5094 5925 6099 6114 7625 9337 96 Size: 1089 831 174 15 1511 1712 138 Fragments arranged by size: 1712 1511 1443 1089 915 831 605 508 274 174 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 9339 9371 2382 Size: 32 6925 32 2390 Fragments arranged by size: 6925 2390 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2125 3564 1316 Size: 809 1439 7131 Fragments arranged by size: 7131 1439 809 XbaI T'CTAG_A Cuts at: 2206 6715 2206 Size: 4509 4870 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3732 5023 6338 6618 3732 Size: 1291 1315 280 6493 Fragments arranged by size: 6493 1315 1291 280 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9379 XmaI C'CCGG_G Cuts at: 1870 6704 1870 Size: 4834 4545 XmnI GAAnn'nnTTC Cuts at: 4253 5154 6032 7508 4253 Size: 901 878 1476 6124 Fragments arranged by size: 6124 1476 901 878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7187 276 Size: 53 83 186 936 5653 2468 Fragments arranged by size: 5653 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI