(Circular) (Six-base) MAPSORT of: pFN21AB6564.seq Check: 8361 from: 1 to: 5905 pFN21AB6564 5905 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2856 3248 1937 Size: 919 392 4594 Fragments arranged by size: 4594 919 392 Acc65I G'GTAC_C Cuts at: 2796 3226 2796 Size: 430 5475 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3104 3968 5352 5620 1632 Size: 1472 864 1384 268 1917 Fragments arranged by size: 1917 1472 1384 864 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 3200 1166 Size: 2034 3871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2368 2479 3648 5220 2368 Size: 111 1169 1572 3053 Fragments arranged by size: 3053 1572 1169 111 ApaI G_GGCC'C Cuts at: 2069 2069 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2284 2501 2906 3230 1870 Size: 73 341 217 405 324 4545 Fragments arranged by size: 4545 405 341 324 217 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2796 2910 3226 4681 Size: 325 217 627 300 709 114 316 1455 Cuts at: 4681 618 Size: 1842 Fragments arranged by size: 1842 1455 709 627 325 316 300 217 114 BanII G_rGCy'C Cuts at: 1391 2069 3224 1391 Size: 678 1155 4072 Fragments arranged by size: 4072 1155 678 BbeI G_GCGC'C Cuts at: 2091 2914 2091 Size: 823 5082 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4096 4130 1903 Size: 34 50 34 2075 34 3678 Fragments arranged by size: 3678 2075 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2096 2908 Size: 107 122 71 631 605 337 87 812 Cuts at: 2908 4516 136 Size: 1608 1525 Fragments arranged by size: 1608 1525 812 631 605 337 122 107 87 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2069 2693 2729 3966 5122 1790 Size: 279 624 36 1237 1156 2573 Fragments arranged by size: 2573 1237 1156 624 279 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2107 2107 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2776 2849 4565 1239 Size: 908 629 73 1716 2579 Fragments arranged by size: 2579 1716 908 629 73 Bpu10I CC'TnA_GC Cuts at: 2997 2997 Size: 5905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 4030 4895 5193 2339 Size: 1691 865 298 3051 Fragments arranged by size: 3051 1691 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2817 2911 3712 Size: 53 83 186 936 555 729 94 801 Cuts at: 3712 4094 4729 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 801 729 635 555 382 186 94 83 53 BsaWI w'CCGG_w Cuts at: 2337 2710 4338 4736 5010 5157 5568 2337 Size: 373 1628 398 274 147 411 2674 Fragments arranged by size: 2674 1628 411 398 373 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2447 2477 1178 Size: 30 165 30 1044 30 4606 Fragments arranged by size: 4606 1044 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2519 1186 Size: 958 375 4572 Fragments arranged by size: 4572 958 375 BseYI C'CCAG_C Cuts at: 2236 3064 5108 5604 2236 Size: 828 2044 496 2537 Fragments arranged by size: 2537 2044 828 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2762 1163 Size: 573 618 408 4306 Fragments arranged by size: 4306 618 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2897 3355 4116 5144 1540 Size: 275 186 896 458 761 1028 2301 Fragments arranged by size: 2301 1028 896 761 458 275 186 BsiHKAI G_wGCw'C Cuts at: 3966 4051 5122 3966 Size: 85 1071 4749 Fragments arranged by size: 4749 1071 85 BsmI GAATG_Cn' Cuts at: 2381 3416 3509 2381 Size: 1035 93 4777 Fragments arranged by size: 4777 1035 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2693 2729 3224 3966 4051 5122 Size: 399 279 624 36 495 742 85 1071 Cuts at: 5122 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 624 495 399 279 85 36 BspEI T'CCGG_A Cuts at: 2337 2710 5568 2337 Size: 373 2858 2674 Fragments arranged by size: 2858 2674 373 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2353 3260 844 Size: 1277 232 907 3489 Fragments arranged by size: 3489 1277 907 232 BsrBI CCG'CTC Cuts at: 1394 1823 2652 2716 3073 3792 1394 Size: 429 829 64 357 719 3507 Fragments arranged by size: 3507 829 719 429 357 64 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 2032 2329 2844 2940 3143 4549 Size: 110 213 9 297 515 96 203 1406 Cuts at: 4549 4736 1700 Size: 187 2869 Fragments arranged by size: 2869 1406 515 297 213 203 187 110 96 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 2606 3965 4977 2606 Size: 1359 1012 3534 Fragments arranged by size: 3534 1359 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 4331 BstBI TT'CG_AA Cuts at: 2220 4709 2220 Size: 2489 3416 BstEII G'GTnAC_C Cuts at: 1184 2260 1184 Size: 1076 4829 BstXI CCAn_nnnn'nTGG Cuts at: 2827 2827 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2226 2508 3235 3602 3987 4004 5456 5542 Size: 254 282 727 367 385 17 1452 86 Cuts at: 5542 5554 5900 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 727 385 367 346 282 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2670 2850 2949 3201 513 Size: 552 1605 180 99 252 3217 Fragments arranged by size: 3217 1605 552 252 180 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2041 2512 487 Size: 683 56 815 471 3880 Fragments arranged by size: 3880 815 683 471 56 BtsI GCAGTG_nn' Cuts at: 3421 4215 4235 3421 Size: 794 20 5091 Fragments arranged by size: 5091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2088 2123 529 Size: 35 1524 35 4311 Fragments arranged by size: 4311 1524 35 35 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2331 2653 Size: 54 1425 50 275 18 468 33 322 Cuts at: 2653 2673 2784 2842 2894 2952 3352 4241 5870 Size: 20 111 58 52 58 400 889 1629 Cuts at: 5870 8 Size: 43 Fragments arranged by size: 1629 1425 889 468 400 322 275 111 58 58 54 52 50 43 33 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2894 3352 1537 Size: 275 1082 458 4090 Fragments arranged by size: 4090 1082 458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3835 1353 Size: 2482 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3092 4492 4866 5012 387 Size: 1064 138 1503 1400 374 146 1280 Fragments arranged by size: 1503 1400 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2776 2849 3104 3968 4565 5352 Size: 393 515 629 73 255 864 597 787 Cuts at: 5352 5620 1239 Size: 268 1524 Fragments arranged by size: 1524 864 787 629 597 515 393 268 255 73 EcoNI CCTnn'n_nnAGG Cuts at: 2471 2471 Size: 5905 EcoO109I rG'GnC_Cy Cuts at: 2065 2365 2409 2901 2065 Size: 300 44 492 5069 Fragments arranged by size: 5069 492 300 44 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FseI GG_CCGG'CC Cuts at: 2848 2848 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2039 2091 2914 5052 1678 Size: 361 52 823 2138 2531 Fragments arranged by size: 2531 2138 823 361 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2447 2479 2610 Size: 32 135 32 482 32 361 32 131 Cuts at: 2610 2642 4546 4578 4620 4652 1373 Size: 32 1904 32 42 32 2626 Fragments arranged by size: 2626 1904 482 361 135 131 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3249 3495 669 Size: 988 281 919 392 246 3079 Fragments arranged by size: 3079 988 919 392 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KasI G'GCGC_C Cuts at: 2087 2910 2087 Size: 823 5082 KpnI G_GTAC'C Cuts at: 2800 3230 2800 Size: 430 5475 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2622 2727 2770 5019 5203 1305 Size: 1317 105 43 2249 184 2007 Fragments arranged by size: 2249 2007 1317 184 105 43 MscI TGG'CCA Cuts at: 10 64 1489 1832 2300 2675 2954 10 Size: 54 1425 343 468 375 279 2961 Fragments arranged by size: 2961 1425 468 375 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 2441 2583 2606 3200 3863 4222 4381 Size: 648 1275 142 23 594 663 359 159 Cuts at: 4381 518 Size: 2042 Fragments arranged by size: 2042 1275 663 648 594 359 159 142 23 MspA1I CmG'CkG Cuts at: 1606 2922 3068 3321 3998 5146 5391 1606 Size: 1316 146 253 677 1148 245 2120 Fragments arranged by size: 2120 1316 1148 677 253 245 146 NaeI GCC'GGC Cuts at: 2034 2331 2846 2942 3145 2034 Size: 297 515 96 203 4794 Fragments arranged by size: 4794 515 297 203 96 NarI GG'CG_CC Cuts at: 2088 2911 2088 Size: 823 5082 NcoI C'CATG_G Cuts at: 513 1065 2670 2949 513 Size: 552 1605 279 3469 Fragments arranged by size: 3469 1605 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 2032 2329 2844 2940 3143 2032 Size: 297 515 96 203 4794 Fragments arranged by size: 4794 515 297 203 96 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2967 3030 2967 Size: 63 5842 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2365 2365 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2065 2065 Size: 5905 PstI C_TGCA'G Cuts at: 830 2118 2364 3257 830 Size: 1288 246 893 3478 Fragments arranged by size: 3478 1288 893 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 RsrII CG'GwC_CG Cuts at: 3117 3117 Size: 5905 SalI G'TCGA_C Cuts at: 1936 2855 3247 1936 Size: 919 392 4594 Fragments arranged by size: 4594 919 392 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SexAI A'CCwGG_T Cuts at: 2103 2472 2103 Size: 369 5536 SfcI C'TryA_G Cuts at: 826 1045 2114 2360 2416 3253 4388 5069 5260 Size: 219 1069 246 56 837 1135 681 191 Cuts at: 5260 826 Size: 1471 Fragments arranged by size: 1471 1135 1069 837 681 246 219 191 56 SfoI GGC'GCC Cuts at: 2089 2912 2089 Size: 823 5082 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 2908 3232 1872 Size: 1036 324 4545 Fragments arranged by size: 4545 1036 324 SmlI C'TyrA_G Cuts at: 820 1017 1943 2318 4009 4910 5172 820 Size: 197 926 375 1691 901 262 1553 Fragments arranged by size: 1691 1553 926 901 375 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2190 2670 2949 3344 513 Size: 552 1125 480 279 395 3074 Fragments arranged by size: 3074 1125 552 480 395 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 3202 3932 3949 4102 4287 4732 Size: 1255 615 1007 730 17 153 185 445 Cuts at: 4732 325 Size: 1498 Fragments arranged by size: 1498 1255 1007 730 615 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4151 5863 96 Size: 274 80 33 51 1443 2174 1712 138 Fragments arranged by size: 2174 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 2615 3241 2615 Size: 626 5279 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2683 2683 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 2906 3230 1870 Size: 1036 324 4545 Fragments arranged by size: 4545 1036 324 XmnI GAAnn'nnTTC Cuts at: 2172 4034 2172 Size: 1862 4043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII SacI SacII SanDI SapI SfiI SrfI SwaI