Restriction map for
pFN21AA0705
(Linear) (Six-base) MAPSORT of: KIAA0705.seq Check: 6483 from: 1 to: 4323
KIAA0705.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 772 1341 2169 4323
Size: 772 569 828 2154
Fragments arranged by size:
2154 828 772 569
AccI GT'mk_AC
Cuts at: 0 953 4323
Size: 953 3370
Acc65I G'GTAC_C
Cuts at: 0 550 4323
Size: 550 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 358 653 868 905 1568 2310 2702
Size: 115 243 295 215 37 663 742 392
Cuts at: 2702 3121 4323
Size: 419 1202
Fragments arranged by size:
1202 742 663 419 392 295 243 215
115 37
AfeI AGC'GCT
Cuts at: 0 3904 4323
Size: 3904 419
AflII C'TTAA_G
Cuts at: 0 1996 4323
Size: 1996 2327
AhdI GACnn_n'nnGTC
Cuts at: 0 1770 4323
Size: 1770 2553
AlwNI CAG_nnn'CTG
Cuts at: 0 1968 2148 2590 4323
Size: 1968 180 442 1733
Fragments arranged by size:
1968 1733 442 180
ApaI G_GGCC'C
Cuts at: 0 4204 4256 4323
Size: 4204 52 67
Fragments arranged by size:
4204 67 52
AseI AT'TA_AT
Cuts at: 0 1427 4323
Size: 1427 2896
AvaI C'yCGr_G
Cuts at: 0 1241 2335 3886 3940 3970 4006 4104 4131
Size: 1241 1094 1551 54 30 36 98 27
Cuts at: 4131 4212 4323
Size: 81 111
Fragments arranged by size:
1551 1241 1094 111 98 81 54 36
30 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 534 567 4323
Size: 534 33 3756
Fragments arranged by size:
3756 534 33
BamHI G'GATC_C
Cuts at: 0 993 2791 2879 4323
Size: 993 1798 88 1444
Fragments arranged by size:
1798 1444 993 88
BanI G'GyrC_C
Cuts at: 0 103 411 550 784 1569 1831 3009 3948
Size: 103 308 139 234 785 262 1178 939
Cuts at: 3948 4002 4059 4219 4263 4303 4323
Size: 54 57 160 44 40 20
Fragments arranged by size:
1178 939 785 308 262 234 160 139
103 57 54 44 40 20
BanII G_rGCy'C
Cuts at: 0 230 2179 2234 2392 2561 2993 4013 4081
Size: 230 1949 55 158 169 432 1020 68
Cuts at: 4081 4088 4204 4256 4323
Size: 7 116 52 67
Fragments arranged by size:
1949 1020 432 230 169 158 116 68
67 55 52 7
BbeI G_GCGC'C
Cuts at: 0 107 3952 4006 4063 4223 4307 4323
Size: 107 3845 54 57 160 84 16
Fragments arranged by size:
3845 160 107 84 57 54 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 776 893 1556 1626 3618 4323
Size: 542 234 117 663 70 1992 705
Fragments arranged by size:
1992 705 663 542 234 117 70
BbvCI CC'TCA_GC
Cuts at: 0 1273 4323
Size: 1273 3050
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 401 4323
Size: 367 34 3922
Fragments arranged by size:
3922 367 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1754 2604 4323
Size: 1754 850 1719
Fragments arranged by size:
1754 1719 850
BclI T'GATC_A
Cuts at: 0 254 4323
Size: 254 4069
BfrBI ATG'CAT
Cuts at: 0 1249 4323
Size: 1249 3074
BglI GCCn_nnn'nGGC
Cuts at: 0 83 3994 4136 4323
Size: 83 3911 142 187
Fragments arranged by size:
3911 187 142 83
BlpI GC'TnA_GC
Cuts at: 0 2391 4323
Size: 2391 1932
Bme1580I G_kGCm'C
Cuts at: 0 1836 2810 4204 4256 4323
Size: 1836 974 1394 52 67
Fragments arranged by size:
1836 1394 974 67 52
BmgBI CAC'GTC
Cuts at: 0 243 4323
Size: 243 4080
BmrI ACTGGGnnnn_n'
Cuts at: 0 930 1248 2654 4323
Size: 930 318 1406 1669
Fragments arranged by size:
1669 1406 930 318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 819 1616 4323
Size: 819 797 2707
Fragments arranged by size:
2707 819 797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 473 1007 1586 3626 3699 4177 4323
Size: 220 253 534 579 2040 73 478 146
Fragments arranged by size:
2040 579 534 478 253 220 146 73
Bpu10I CC'TnA_GC
Cuts at: 0 1273 1969 4323
Size: 1273 696 2354
Fragments arranged by size:
2354 1273 696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 3616 4323
Size: 268 3348 707
Fragments arranged by size:
3348 707 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 2867 4323
Size: 205 2662 1456
Fragments arranged by size:
2662 1456 205
BsaAI yAC'GTr
Cuts at: 0 1742 4323
Size: 1742 2581
BsaBI GATnn'nnATC
Cuts at: 0 2294 4323
Size: 2294 2029
BsaHI Gr'CG_yC
Cuts at: 0 104 2268 3722 3949 4003 4060 4220 4304
Size: 104 2164 1454 227 54 57 160 84
Cuts at: 4304 4323
Size: 19
Fragments arranged by size:
2164 1454 227 160 104 84 57 54
19
BsaWI w'CCGG_w
Cuts at: 0 1368 2876 4323
Size: 1368 1508 1447
Fragments arranged by size:
1508 1447 1368
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 293 323 728 758 2161 2191 4323
Size: 293 30 405 30 1403 30 2132
Fragments arranged by size:
2132 1403 405 293 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 712 856 2057 3001 3958 4323
Size: 202 510 144 1201 944 957 365
Fragments arranged by size:
1201 957 944 510 365 202 144
BseYI C'CCAG_C
Cuts at: 0 81 806 1058 3013 3077 3087 3113 4285
Size: 81 725 252 1955 64 10 26 1172
Cuts at: 4285 4323
Size: 38
Fragments arranged by size:
1955 1172 725 252 81 64 38 26
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 803 1690 4323
Size: 800 3 887 2633
Fragments arranged by size:
2633 887 800 3
BsiEI CG_ry'CG
Cuts at: 0 1693 3934 4198 4313 4323
Size: 1693 2241 264 115 10
Fragments arranged by size:
2241 1693 264 115 10
BsiHKAI G_wGCw'C
Cuts at: 0 513 2179 2234 2993 4088 4323
Size: 513 1666 55 759 1095 235
Fragments arranged by size:
1666 1095 759 513 235 55
BsmI GAATG_Cn'
Cuts at: 0 3437 4323
Size: 3437 886
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1322 3729 4323
Size: 1322 2407 594
Fragments arranged by size:
2407 1322 594
Bsp1286I G_dGCh'C
Cuts at: 0 230 513 1836 2179 2234 2392 2561 2810
Size: 230 283 1323 343 55 158 169 249
Cuts at: 2810 2993 4013 4081 4088 4204 4256 4323
Size: 183 1020 68 7 116 52 67
Fragments arranged by size:
1323 1020 343 283 249 230 183 169
158 116 68 67 55 52 7
BspEI T'CCGG_A
Cuts at: 0 1368 4323
Size: 1368 2955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 772 1341 2156 2169 2505 2941 4323
Size: 772 569 815 13 336 436 1382
Fragments arranged by size:
1382 815 772 569 436 336 13
BsrBI CCG'CTC
Cuts at: 0 167 4323
Size: 167 4156
BsrDI GCAATG_nn'
Cuts at: 0 907 2378 2514 2673 4323
Size: 907 1471 136 159 1650
Fragments arranged by size:
1650 1471 907 159 136
BsrFI r'CCGG_y
Cuts at: 0 2458 3976 4091 4116 4126 4323
Size: 2458 1518 115 25 10 197
Fragments arranged by size:
2458 1518 197 115 25 10
BsrGI T'GTAC_A
Cuts at: 0 827 1959 4323
Size: 827 1132 2364
Fragments arranged by size:
2364 1132 827
BssHII G'CGCG_C
Cuts at: 0 4151 4180 4182 4228 4323
Size: 4151 29 2 46 95
Fragments arranged by size:
4151 95 46 29 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1713 2148 4323
Size: 1713 435 2175
Fragments arranged by size:
2175 1713 435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1058 2718 4323
Size: 1058 1660 1605
Fragments arranged by size:
1660 1605 1058
BstYI r'GATC_y
Cuts at: 0 993 2791 2879 4323
Size: 993 1798 88 1444
Fragments arranged by size:
1798 1444 993 88
Bsu36I CC'TnA_GG
Cuts at: 0 695 789 839 2981 4323
Size: 695 94 50 2142 1342
Fragments arranged by size:
2142 1342 695 94 50
BtgI C'CryG_G
Cuts at: 0 218 2059 2209 2384 3035 4323
Size: 218 1841 150 175 651 1288
Fragments arranged by size:
1841 1288 651 218 175 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3093 4323
Size: 3093 1230
BtsI GCAGTG_nn'
Cuts at: 0 261 2625 2894 4323
Size: 261 2364 269 1429
Fragments arranged by size:
2364 1429 269 261
ClaI AT'CG_AT
Cuts at: 0 1070 4323
Size: 1070 3253
DraIII CAC_nnn'GTG
Cuts at: 0 2620 4323
Size: 2620 1703
DrdI GACnn_nn'nnGTC
Cuts at: 0 1665 4323
Size: 1665 2658
EaeI y'GGCC_r
Cuts at: 0 248 1690 2132 2406 2895 3867 3931 3957
Size: 248 1442 442 274 489 972 64 26
Cuts at: 3957 4195 4310 4323
Size: 238 115 13
Fragments arranged by size:
1442 972 489 442 274 248 238 115
64 26 13
EagI C'GGCC_G
Cuts at: 0 1690 3931 4195 4310 4323
Size: 1690 2241 264 115 13
Fragments arranged by size:
2241 1690 264 115 13
EarI CTCTTCn'nnn_
Cuts at: 0 640 698 704 1022 1240 4323
Size: 640 58 6 318 218 3083
Fragments arranged by size:
3083 640 318 218 58 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4045 4323
Size: 4045 278
EcoICRI GAG'CTC
Cuts at: 0 2177 2232 2991 4086 4323
Size: 2177 55 759 1095 237
Fragments arranged by size:
2177 1095 759 237 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 220 358 473 653 868 905 1007
Size: 115 105 138 115 180 215 37 102
Cuts at: 1007 1568 1586 2310 2702 3121 3626 3699 4177
Size: 561 18 724 392 419 505 73 478
Cuts at: 4177 4323
Size: 146
Fragments arranged by size:
724 561 505 478 419 392 215 180
146 138 115 115 105 102 73 37
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 4323
Size: 133 4190
EcoO109I rG'GnC_Cy
Cuts at: 0 270 447 1209 3651 4189 4252 4253 4323
Size: 270 177 762 2442 538 63 1 70
Fragments arranged by size:
2442 762 538 270 177 70 63 1
EcoRV GAT'ATC
Cuts at: 0 3819 4323
Size: 3819 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 545 1822 2039 4323
Size: 545 1277 217 2284
Fragments arranged by size:
2284 1277 545 217
HaeII r_GCGC'y
Cuts at: 0 107 3885 3906 3952 4006 4063 4223 4307
Size: 107 3778 21 46 54 57 160 84
Cuts at: 4307 4323
Size: 16
Fragments arranged by size:
3778 160 107 84 57 54 46 21
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 1756 1788 2022 2054 2776 2808
Size: 1301 32 423 32 234 32 722 32
Cuts at: 2808 2864 2896 3521 3553 4323
Size: 56 32 625 32 770
Fragments arranged by size:
1301 770 722 625 423 234 56 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1101 1506 2520 4323
Size: 1101 405 1014 1803
Fragments arranged by size:
1803 1101 1014 405
HindIII A'AGCT_T
Cuts at: 0 9 4323
Size: 9 4314
KasI G'GCGC_C
Cuts at: 0 103 3948 4002 4059 4219 4303 4323
Size: 103 3845 54 57 160 84 20
Fragments arranged by size:
3845 160 103 84 57 54 20
KpnI G_GTAC'C
Cuts at: 0 554 4323
Size: 554 3769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 3231 3341 3803 4323
Size: 164 3067 110 462 520
Fragments arranged by size:
3067 520 462 164 110
MscI TGG'CCA
Cuts at: 0 2897 4323
Size: 2897 1426
MslI CAynn'nnrTG
Cuts at: 0 50 1454 1460 1628 2383 2474 2911 4323
Size: 50 1404 6 168 755 91 437 1412
Fragments arranged by size:
1412 1404 755 437 168 91 50 6
MspA1I CmG'CkG
Cuts at: 0 81 1878 3141 3243 3691 4148 4323
Size: 81 1797 1263 102 448 457 175
Fragments arranged by size:
1797 1263 457 448 175 102 81
NaeI GCC'GGC
Cuts at: 0 2460 3978 4093 4118 4128 4323
Size: 2460 1518 115 25 10 195
Fragments arranged by size:
2460 1518 195 115 25 10
NarI GG'CG_CC
Cuts at: 0 104 3949 4003 4060 4220 4304 4323
Size: 104 3845 54 57 160 84 19
Fragments arranged by size:
3845 160 104 84 57 54 19
NcoI C'CATG_G
Cuts at: 0 2059 2384 3035 4323
Size: 2059 325 651 1288
Fragments arranged by size:
2059 1288 651 325
NdeI CA'TA_TG
Cuts at: 0 1053 2226 4323
Size: 1053 1173 2097
Fragments arranged by size:
2097 1173 1053
NgoMIV G'CCGG_C
Cuts at: 0 2458 3976 4091 4116 4126 4323
Size: 2458 1518 115 25 10 197
Fragments arranged by size:
2458 1518 197 115 25 10
NsiI A_TGCA'T
Cuts at: 0 1251 4323
Size: 1251 3072
NspI r_CATG'y
Cuts at: 0 1176 2916 3580 4323
Size: 1176 1740 664 743
Fragments arranged by size:
1740 1176 743 664
PasI CC'CwG_GG
Cuts at: 0 1836 4323
Size: 1836 2487
PflMI CCAn_nnn'nTGG
Cuts at: 0 1723 4323
Size: 1723 2600
PfoI T'CCnGG_A
Cuts at: 0 612 1139 4323
Size: 612 527 3184
Fragments arranged by size:
3184 612 527
PpuMI rG'GwC_Cy
Cuts at: 0 270 447 3651 4189 4323
Size: 270 177 3204 538 134
Fragments arranged by size:
3204 538 270 177 134
PshAI GACnn'nnGTC
Cuts at: 0 323 4323
Size: 323 4000
PsiI TTA'TAA
Cuts at: 0 2279 4323
Size: 2279 2044
PspOMI G'GGCC_C
Cuts at: 0 4200 4252 4323
Size: 4200 52 71
Fragments arranged by size:
4200 71 52
PspXI vC'TCGA_Gb
Cuts at: 0 3940 4323
Size: 3940 383
PstI C_TGCA'G
Cuts at: 0 1208 1352 1383 2708 2847 4323
Size: 1208 144 31 1325 139 1476
Fragments arranged by size:
1476 1325 1208 144 139 31
PvuII CAG'CTG
Cuts at: 0 81 3141 4323
Size: 81 3060 1182
Fragments arranged by size:
3060 1182 81
SacI G_AGCT'C
Cuts at: 0 2179 2234 2993 4088 4323
Size: 2179 55 759 1095 235
Fragments arranged by size:
2179 1095 759 235 55
SanDI GG'GwC_CC
Cuts at: 0 4189 4323
Size: 4189 134
SbfI CC_TGCA'GG
Cuts at: 0 1208 4323
Size: 1208 3115
SexAI A'CCwGG_T
Cuts at: 0 1508 4323
Size: 1508 2815
SfcI C'TryA_G
Cuts at: 0 1159 1204 1348 1379 1868 2704 2843 3228
Size: 1159 45 144 31 489 836 139 385
Cuts at: 3228 3297 3321 3366 4323
Size: 69 24 45 957
Fragments arranged by size:
1159 957 836 489 385 144 139 69
45 45 31 24
SfoI GGC'GCC
Cuts at: 0 105 3950 4004 4061 4221 4305 4323
Size: 105 3845 54 57 160 84 18
Fragments arranged by size:
3845 160 105 84 57 54 18
SgrAI Cr'CCGG_yG
Cuts at: 0 4116 4323
Size: 4116 207
SmaI CCC'GGG
Cuts at: 0 1243 4008 4106 4133 4214 4323
Size: 1243 2765 98 27 81 109
Fragments arranged by size:
2765 1243 109 98 81 27
SmlI C'TyrA_G
Cuts at: 0 283 1996 3631 3940 4323
Size: 283 1713 1635 309 383
Fragments arranged by size:
1713 1635 383 309 283
SrfI GCCC'GGGC
Cuts at: 0 4008 4133 4323
Size: 4008 125 190
Fragments arranged by size:
4008 190 125
StuI AGG'CCT
Cuts at: 0 717 2200 2781 4323
Size: 717 1483 581 1542
Fragments arranged by size:
1542 1483 717 581
StyI C'CwwG_G
Cuts at: 0 1443 2059 2384 3035 3290 4323
Size: 1443 616 325 651 255 1033
Fragments arranged by size:
1443 1033 651 616 325 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1810 2102 2432 2614 3027 4323
Size: 1810 292 330 182 413 1296
Fragments arranged by size:
1810 1296 413 330 292 182
TatI w'GTAC_w
Cuts at: 0 827 1646 1959 4323
Size: 827 819 313 2364
Fragments arranged by size:
2364 827 819 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1739 1771 1865 1897 2161 2193 3602 3634
Size: 1739 32 94 32 264 32 1409 32
Cuts at: 3634 4323
Size: 689
Fragments arranged by size:
1739 1409 689 264 94 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1186 3809 4323
Size: 1186 2623 514
Fragments arranged by size:
2623 1186 514
XhoI C'TCGA_G
Cuts at: 0 3940 4323
Size: 3940 383
XmaI C'CCGG_G
Cuts at: 0 1241 4006 4104 4131 4212 4323
Size: 1241 2765 98 27 81 111
Fragments arranged by size:
2765 1241 111 98 81 27
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AhdI AlwNI
ApaI AseI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuII SacI SanDI SbfI SexAI SfcI
SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflIII AgeI AleI AlfI AloI ApaLI
ApoI AscI AsiSI AvrII BglII BmtI BsiWI BspHI
BssSI BstBI BstEII BstZ17I CspCI DraI EcoRI FseI
FspI FspAI HpaI MfeI MluI NheI NotI NruI
PacI PciI PmeI PmlI PpiI PsrI PvuI RsrII
SacII SalI SapI ScaI SfiI SgfI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI XmnI ZraI