(Circular) (Six-base) MAPSORT of: pFN21AE0208.seq Check: 6981 from: 1 to: 5818 pFN21AE0208 5818 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2916 3161 1937 Size: 979 245 4594 Fragments arranged by size: 4594 979 245 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3881 5265 5533 1632 Size: 2249 1384 268 1917 Fragments arranged by size: 2249 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2238 4717 5694 1203 Size: 1035 2479 977 1327 Fragments arranged by size: 2479 1327 1035 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 1184 Size: 1299 4519 AlwNI CAG_nnn'CTG Cuts at: 2221 2446 3561 5133 2221 Size: 225 1115 1572 2906 Fragments arranged by size: 2906 1572 1115 225 ApaI G_GGCC'C Cuts at: 2322 2322 Size: 5818 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 2530 2945 3127 3353 3505 5614 1493 Size: 1037 415 182 226 152 2109 1697 Fragments arranged by size: 2109 1697 1037 415 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2252 2688 3143 1870 Size: 73 309 436 455 4545 Fragments arranged by size: 4545 455 436 309 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2860 2893 2860 Size: 33 5785 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 3139 4594 618 Size: 325 217 627 620 732 1455 1842 Fragments arranged by size: 1842 1455 732 627 620 325 217 BanII G_rGCy'C Cuts at: 1391 2322 3137 1391 Size: 931 815 4072 Fragments arranged by size: 4072 931 815 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2651 928 Size: 971 752 4095 Fragments arranged by size: 4095 971 752 BbvCI CC'TCA_GC Cuts at: 2066 2384 2066 Size: 318 5500 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 2457 4429 Size: 107 122 71 631 605 370 415 1972 Cuts at: 4429 136 Size: 1525 Fragments arranged by size: 1972 1525 631 605 415 370 122 107 71 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 2322 3879 5035 1790 Size: 532 1557 1156 2573 Fragments arranged by size: 2573 1557 1156 532 BmgBI CAC'GTC Cuts at: 1318 2227 1318 Size: 909 4909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2316 2341 3549 Size: 416 53 83 399 24 899 25 1208 Cuts at: 3549 4507 442 Size: 958 1753 Fragments arranged by size: 1753 1208 958 899 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2714 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2120 2198 2354 4478 1239 Size: 869 12 78 156 2124 2579 Fragments arranged by size: 2579 2124 869 156 78 12 Bpu10I CC'TnA_GC Cuts at: 2066 2384 2447 2066 Size: 318 63 5437 Fragments arranged by size: 5437 318 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3625 4007 4642 275 Size: 53 83 186 936 2092 382 635 1451 Fragments arranged by size: 2092 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2040 1178 Size: 30 165 30 607 30 4956 Fragments arranged by size: 4956 607 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2129 2610 2925 1186 Size: 838 105 481 315 4079 Fragments arranged by size: 4079 838 481 315 105 BseYI C'CCAG_C Cuts at: 2191 5021 5517 2191 Size: 2830 496 2492 Fragments arranged by size: 2830 2492 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 3072 1163 Size: 573 1051 285 3909 Fragments arranged by size: 3909 1051 573 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 3268 4029 5057 1540 Size: 275 186 36 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2379 3879 3964 5035 2379 Size: 1500 85 1071 3162 Fragments arranged by size: 3162 1500 1071 85 BsmI GAATG_Cn' Cuts at: 3102 3329 3422 3102 Size: 227 93 5498 Fragments arranged by size: 5498 227 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2075 2075 Size: 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2379 3137 3879 3964 5035 1391 Size: 399 532 57 758 742 85 1071 2174 Fragments arranged by size: 2174 1071 758 742 532 399 85 57 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 844 Size: 2329 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2119 2232 2356 3705 1394 Size: 429 296 113 124 1349 3507 Fragments arranged by size: 3507 1349 429 296 124 113 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2307 4462 4649 1700 Size: 110 497 2155 187 2869 Fragments arranged by size: 2869 2155 497 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 2431 2433 1925 Size: 506 2 5310 Fragments arranged by size: 5310 506 2 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 2821 4622 2821 Size: 1801 4017 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2341 Size: 5818 BstYI r'GATC_y Cuts at: 1972 2694 3148 3515 3900 3917 5369 5455 5467 Size: 722 454 367 385 17 1452 86 12 Cuts at: 5467 5813 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 722 454 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2183 513 Size: 552 939 179 4148 Fragments arranged by size: 4148 939 552 179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2089 2930 4825 809 Size: 507 773 841 1895 1802 Fragments arranged by size: 1895 1802 841 773 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2295 3265 Size: 54 1425 50 275 18 204 261 970 Cuts at: 3265 4154 5783 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 970 889 275 261 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 3265 1537 Size: 275 222 1231 4090 Fragments arranged by size: 4090 1231 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2712 3748 1353 Size: 1359 1036 3423 Fragments arranged by size: 3423 1359 1036 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2120 2198 2354 3881 4478 5265 Size: 393 476 12 78 156 1527 597 787 Cuts at: 5265 5533 1239 Size: 268 1524 Fragments arranged by size: 1527 1524 787 597 476 393 268 156 78 12 EcoO109I rG'GnC_Cy Cuts at: 2338 2338 Size: 5818 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3128 3128 Size: 5818 FspI TGC'GCA Cuts at: 2285 4324 2285 Size: 2039 3779 HaeII r_GCGC'y Cuts at: 1678 2141 2192 4965 1678 Size: 463 51 2773 2531 Fragments arranged by size: 2773 2531 463 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4459 4491 4533 4565 1373 Size: 32 135 32 2887 32 42 32 2626 Fragments arranged by size: 2887 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2244 3162 3408 669 Size: 988 281 306 918 246 3079 Fragments arranged by size: 3079 988 918 306 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 2887 3417 2887 Size: 530 5288 MluI A'CGCG_T Cuts at: 2238 5694 2238 Size: 3456 2362 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2807 4932 5116 1305 Size: 1502 2125 184 2007 Fragments arranged by size: 2125 2007 1502 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3531 Fragments arranged by size: 3531 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3004 3776 4135 4294 518 Size: 648 1838 772 359 159 2042 Fragments arranged by size: 2042 1838 772 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2199 2218 2388 3234 3911 5059 5304 Size: 418 175 19 170 846 677 1148 245 Cuts at: 5304 1606 Size: 2120 Fragments arranged by size: 2120 1148 846 677 418 245 175 170 19 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4327 Fragments arranged by size: 4327 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 3176 4721 1132 Size: 97 1947 1545 2229 Fragments arranged by size: 2229 1947 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 2906 1108 Size: 1113 685 4020 Fragments arranged by size: 4020 1113 685 PfoI T'CCnGG_A Cuts at: 2400 2790 2851 2400 Size: 390 61 5367 Fragments arranged by size: 5367 390 61 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspOMI G'GGCC_C Cuts at: 2318 2318 Size: 5818 PstI C_TGCA'G Cuts at: 830 2442 2472 3170 830 Size: 1612 30 698 3478 Fragments arranged by size: 3478 1612 698 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 2218 2218 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 2438 2468 3166 4301 4982 5173 826 Size: 219 1393 30 698 1135 681 191 1471 Fragments arranged by size: 1471 1393 1135 698 681 219 191 30 SfiI GGCCn_nnn'nGGCC Cuts at: 2042 2042 Size: 5818 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 3145 1872 Size: 1273 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2688 3922 4823 5085 820 Size: 197 926 745 1234 901 262 1553 Fragments arranged by size: 1553 1234 926 901 745 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 2827 3742 5 Size: 47 2775 915 2081 Fragments arranged by size: 2775 2081 915 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5818 StyI C'CwwG_G Cuts at: 513 1065 2004 2334 3257 513 Size: 552 939 330 923 3074 Fragments arranged by size: 3074 939 923 552 330 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4064 5776 96 Size: 274 80 33 51 1443 2087 1712 138 Fragments arranged by size: 2087 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2010 2042 5778 5810 2010 Size: 32 3736 32 2018 Fragments arranged by size: 3736 2018 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 2083 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 2688 1943 Size: 745 5073 XmaI C'CCGG_G Cuts at: 1870 3143 1870 Size: 1273 4545 XmnI GAAnn'nnTTC Cuts at: 2827 3947 2827 Size: 1120 4698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI