(Circular) (Six-base) MAPSORT of: pFN21AE0556.seq Check: 1972 from: 1 to: 5458 pFN21AE0556 5458 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3268 278 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 1937 Size: 864 4594 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2150 3521 4905 5173 1632 Size: 518 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 518 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 4357 5334 1203 Size: 3154 977 1327 Fragments arranged by size: 3154 1327 977 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 2159 4187 2159 Size: 2028 3430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5458 AlwNI CAG_nnn'CTG Cuts at: 2044 2058 2174 3201 4773 2044 Size: 14 116 1027 1572 2729 Fragments arranged by size: 2729 1572 1027 116 14 ApaI G_GGCC'C Cuts at: 2197 2594 2659 2197 Size: 397 65 4996 Fragments arranged by size: 4996 397 65 ApaLI G'TGCA_C Cuts at: 3515 4671 3515 Size: 1156 4302 ApoI r'AATT_y Cuts at: 1493 2606 2767 2993 3145 5254 1493 Size: 1113 161 226 152 2109 1697 Fragments arranged by size: 2109 1697 1113 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2783 1870 Size: 73 840 4545 Fragments arranged by size: 4545 840 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2779 4234 618 Size: 325 217 627 992 1455 1842 Fragments arranged by size: 1842 1455 992 627 325 217 BanII G_rGCy'C Cuts at: 1391 2118 2197 2594 2659 2777 1391 Size: 727 79 397 65 118 4072 Fragments arranged by size: 4072 727 397 118 79 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BbvCI CC'TCA_GC Cuts at: 2221 2502 2573 2221 Size: 281 71 5106 Fragments arranged by size: 5106 281 71 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 2225 4069 Size: 107 122 71 631 605 450 103 1844 Cuts at: 4069 136 Size: 1525 Fragments arranged by size: 1844 1525 631 605 450 122 107 103 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 2038 2197 2594 2659 3519 4675 1790 Size: 248 159 397 65 860 1156 2573 Fragments arranged by size: 2573 1156 860 397 248 159 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5458 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4118 1239 Size: 2879 2579 Bpu10I CC'TnA_GC Cuts at: 2221 2502 2573 2221 Size: 281 71 5106 Fragments arranged by size: 5106 281 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4448 4746 3583 Size: 865 298 4295 Fragments arranged by size: 4295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2372 4121 882 Size: 617 873 1749 2219 Fragments arranged by size: 2219 1749 873 617 BsaAI yAC'GTr Cuts at: 493 1204 2671 3249 493 Size: 711 1467 578 2702 Fragments arranged by size: 2702 1467 711 578 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3265 3647 4282 275 Size: 53 83 186 936 1732 382 635 1451 Fragments arranged by size: 1732 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3891 4289 4563 4710 5121 3891 Size: 398 274 147 411 4228 Fragments arranged by size: 4228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5233 Fragments arranged by size: 5233 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2480 2590 1186 Size: 1090 204 110 4054 Fragments arranged by size: 4054 1090 204 110 BseYI C'CCAG_C Cuts at: 2062 2296 2509 2587 2752 4661 5157 2062 Size: 234 213 78 165 1909 496 2363 Fragments arranged by size: 2363 1909 496 234 213 165 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 2573 2693 1163 Size: 573 335 502 120 3928 Fragments arranged by size: 3928 573 502 335 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3669 4697 1540 Size: 275 186 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3519 3604 4675 3519 Size: 85 1071 4302 Fragments arranged by size: 4302 1071 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2118 2197 2594 2659 2777 3519 Size: 399 248 80 79 397 65 118 742 Cuts at: 3519 3604 4675 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 248 118 85 80 79 65 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2813 844 Size: 1969 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3345 1394 Size: 429 1522 3507 Fragments arranged by size: 3507 1522 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2208 3953 4127 65 Size: 1183 960 1745 174 1396 Fragments arranged by size: 1745 1396 1183 960 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 4102 4289 1700 Size: 110 238 2054 187 2869 Fragments arranged by size: 2869 2054 238 187 110 BsrGI T'GTAC_A Cuts at: 96 2319 96 Size: 2223 3235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 794 Size: 1919 3539 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 2206 1184 Size: 1022 4436 BstYI r'GATC_y Cuts at: 1972 2721 2788 3155 3540 3557 5009 5095 5107 Size: 749 67 367 385 17 1452 86 12 Cuts at: 5107 5453 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 749 385 367 346 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 2281 2328 513 Size: 552 939 228 49 47 3643 Fragments arranged by size: 3643 939 552 228 49 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4719 Fragments arranged by size: 4719 683 56 BtsI GCAGTG_nn' Cuts at: 2974 3768 3788 2974 Size: 794 20 4644 Fragments arranged by size: 4644 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2374 2905 3794 Size: 54 1425 50 275 18 544 531 889 Cuts at: 3794 5423 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 544 531 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2905 1537 Size: 275 1093 4090 Fragments arranged by size: 4090 1093 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 3388 1353 Size: 929 1106 3423 Fragments arranged by size: 3423 1106 929 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4045 4419 4565 387 Size: 1064 138 2456 374 146 1280 Fragments arranged by size: 2456 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 3521 4118 4905 5173 1239 Size: 393 518 1371 597 787 268 1524 Fragments arranged by size: 1524 1371 787 597 518 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 5458 EcoO109I rG'GnC_Cy Cuts at: 2067 2194 2286 2305 2591 2656 2067 Size: 127 92 19 286 65 4869 Fragments arranged by size: 4869 286 127 92 65 19 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FspI TGC'GCA Cuts at: 2108 3964 2108 Size: 1856 3602 HaeII r_GCGC'y Cuts at: 1678 2583 2750 4605 1678 Size: 905 167 1855 2531 Fragments arranged by size: 2531 1855 905 167 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2145 2177 4099 4131 4173 Size: 32 135 32 573 32 1922 32 42 Cuts at: 4173 4205 1373 Size: 32 2626 Fragments arranged by size: 2626 1922 573 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2802 3048 669 Size: 988 281 864 246 3079 Fragments arranged by size: 3079 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 4572 4756 1305 Size: 1125 2142 184 2007 Fragments arranged by size: 2142 2007 1125 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 3416 3775 3934 518 Size: 648 1002 1248 359 159 2042 Fragments arranged by size: 2042 1248 1002 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2225 2296 2482 2501 2557 2572 2677 2744 Size: 619 71 186 19 56 15 105 67 Cuts at: 2744 2874 3551 4699 4944 1606 Size: 130 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 619 245 186 130 105 71 67 56 19 15 NcoI C'CATG_G Cuts at: 513 1065 2004 2232 2281 513 Size: 552 939 228 49 3690 Fragments arranged by size: 3690 939 552 228 49 NdeI CA'TA_TG Cuts at: 387 387 Size: 5458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2816 4361 1132 Size: 97 1587 1545 2229 Fragments arranged by size: 2229 1587 1545 97 PasI CC'CwG_GG Cuts at: 1348 2030 1348 Size: 682 4776 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5458 PfoI T'CCnGG_A Cuts at: 2724 2724 Size: 5458 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PmlI CAC'GTG Cuts at: 2671 2671 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3527 3559 5040 5072 3527 Size: 32 1481 32 3913 Fragments arranged by size: 3913 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2067 2286 2067 Size: 219 5239 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5458 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PspOMI G'GGCC_C Cuts at: 2193 2590 2655 2193 Size: 397 65 4996 Fragments arranged by size: 4996 397 65 PstI C_TGCA'G Cuts at: 830 2295 2481 2487 2810 830 Size: 1465 186 6 323 3478 Fragments arranged by size: 3478 1465 323 186 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 PvuII CAG'CTG Cuts at: 2296 2482 2501 2572 2296 Size: 186 19 71 5182 Fragments arranged by size: 5182 186 71 19 SalI G'TCGA_C Cuts at: 1936 2800 1936 Size: 864 4594 SanDI GG'GwC_CC Cuts at: 2286 2286 Size: 5458 SapI GCTCTTCn'nnn_ Cuts at: 2282 2282 Size: 5458 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SfcI C'TryA_G Cuts at: 826 1045 2291 2477 2483 2806 3941 4622 4813 Size: 219 1246 186 6 323 1135 681 191 Cuts at: 4813 826 Size: 1471 Fragments arranged by size: 1471 1246 1135 681 323 219 191 186 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5458 SmaI CCC'GGG Cuts at: 1872 2785 1872 Size: 913 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3562 4463 4725 820 Size: 197 926 1619 901 262 1553 Fragments arranged by size: 1619 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2816 1132 Size: 1684 3774 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5458 StyI C'CwwG_G Cuts at: 513 1065 2004 2232 2281 2897 513 Size: 552 939 228 49 616 3074 Fragments arranged by size: 3074 939 616 552 228 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3485 3502 3655 3840 4285 325 Size: 1255 1905 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2319 3704 Size: 274 80 33 51 1443 105 237 1385 Cuts at: 3704 5416 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1385 274 237 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5418 5450 5418 Size: 32 5426 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2359 Size: 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2783 1870 Size: 913 4545 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3266 276 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI