Restriction map for
pFN21AE0449
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tsp45I Tsp509I Tth111I TthHB8I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 281 GTAGAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AhdI 284 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 284 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 188 GAGCTC G[GA]GC[CT]^C
BbvCI 747 GCTGAGG CCTCAGC(-5/-2)
BcnI 740 CCGGG CC^[GC]GG
BglII 803 AGATCT A^GATCT
BsaI 341 GGTCTC GGTCTC(1/5)
BseRI 532 GAGGAG GAGGAG(10/8)
BsmAI 342 GTCTC GTCTC(1/5)
BspMI 560 ACCTGC ACCTGC(4/8)
BsrBI 582 GAGCGG CCGCTC(-3/-3)
BstAPI 701 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 13 CGCG CG^CG
BstYI 803 AGATCT [GA]^GATC[CT]
DpnII 804 GATC ^GATC
DsaI 612 CCGTGG C^C[GA][CT]GG
Eam1105I 284 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 95 CTCTTC CTCTTC(1/4)
EcoNI 94 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 740 CCGG C^CGG
HindIII 141 AAGCTT A^AGCTT
HpaII 740 CCGG C^CGG
Ksp632I 95 CTCTTC CTCTTC(1/4)
MaeII 380 ACGT A^CGT
MboI 804 GATC ^GATC
MflI 803 AGATCT [GA]^GATC[CT]
MseI 301 TTAA T^TAA
MspA1I 85 CAGCTG C[AC]G^C[GT]G
MspI 740 CCGG C^CGG
MvnI 13 CGCG CG^CG
NciI 740 CCGGG CC^[GC]GG
NdeII 804 GATC ^GATC
NspI 134 ACATGC [GA]CATG^[CT]
PflMI 471 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 85 CAGCTG CAG^CTG
SacI 188 GAGCTC GAGCT^C
Sau3AI 804 GATC ^GATC
SexAI 108 ACCAGGT A^CC[AT]GGT
TfiI 241 GAATC G^A[AT]TC
Tru9I 301 TTAA T^TAA
Van91I 471 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 803 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 49 GGCGTC G[GA]^CG[CT]C
284 GACGCC
AlwNI 239 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
702 CAGCCCCTG
AspHI 188 GAGCTC G[AT]GC[AT]^C
588 GAGCAC
AvaI 66 CTCGGG C^[CT]CG[GA]G
627 CTCGGG
AvaII 652 GGACC G^G[AT]CC
754 GGTCC
BmyI 188 GAGCTC G[AGT]GC[ACT]^C
588 GAGCAC
BpmI 712 CTCCAG CTGGAG(16/14)
478 CTGGAG
BsaHI 49 GGCGTC G[GA]^CG[CT]C
284 GACGCC
BsiHKAI 188 GAGCTC G[AT]GC[AT]^C
588 GAGCAC
BsoBI 66 CTCGGG C^[CT]CG[GA]G
627 CTCGGG
Bsp1286I 188 GAGCTC G[AGT]GC[ACT]^C
588 GAGCAC
BsrI 477 ACTGG ACTGG(1/-1)
365 CCAGT
BstXI 390 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
783 CCAGCAGGATGG
FauI 545 CCCGC CCCGC(4/6)
584 GCGGG
HaeIII 541 GGCC GG^CC
673 GGCC
HgaI 284 GACGC GACGC(5/10)
50 GCGTC
Hin1I 49 GGCGTC G[GA]^CG[CT]C
284 GACGCC
HphI 259 GGTGA GGTGA(8/7)
687 GGTGA
PpuMI 651 GGGACCC [GA]G^G[AT]CC[CT]
753 GGGTCCT
XbaI 345 TCTAGA T^CTAGA
456 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BbvI BfaI BsaJI
BsgI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HhaI HinP1I HinfI ItaI
MaeI MboII MnlI MvaI MwoI
NlaIII NlaIV PleI PstI Sau96I
ScrFI SfcI TseI TspRI