(Circular) (Six-base) MAPSORT of: pFN21AE0449.seq Check: 4383 from: 1 to: 5518 pFN21AE0449 5518 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2287 2861 1937 Size: 350 574 4594 Fragments arranged by size: 4594 574 350 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 2205 2474 2539 3581 4965 5233 1632 Size: 414 159 269 65 1042 1384 268 1917 Fragments arranged by size: 1917 1384 1042 414 269 268 159 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 2294 4247 2294 Size: 1953 3565 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AlwNI CAG_nnn'CTG Cuts at: 2249 2712 3261 4833 2249 Size: 463 549 1572 2934 Fragments arranged by size: 2934 1572 549 463 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2632 2843 1870 Size: 73 128 561 211 4545 Fragments arranged by size: 4545 561 211 128 73 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2839 4294 618 Size: 325 217 627 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 325 217 BanII G_rGCy'C Cuts at: 1391 2197 2837 1391 Size: 806 640 4072 Fragments arranged by size: 4072 806 640 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4547 BbvCI CC'TCA_GC Cuts at: 2753 2753 Size: 5518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3709 3743 1903 Size: 34 50 34 1688 34 3678 Fragments arranged by size: 3678 1688 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 136 Size: 107 122 71 631 605 2457 1525 Fragments arranged by size: 2457 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2808 5513 2808 Size: 2705 2813 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 3579 4735 1790 Size: 1789 1156 2573 Fragments arranged by size: 2573 1789 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2702 4178 1239 Size: 1265 198 1476 2579 Fragments arranged by size: 2579 1476 1265 198 Bpu10I CC'TnA_GC Cuts at: 2753 2753 Size: 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2352 4181 882 Size: 617 853 1829 2219 Fragments arranged by size: 2219 1829 853 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2290 3325 3707 Size: 53 83 186 936 522 235 1035 382 Cuts at: 3707 4342 275 Size: 635 1451 Fragments arranged by size: 1451 1035 936 635 522 382 235 186 83 53 BsaWI w'CCGG_w Cuts at: 3951 4349 4623 4770 5181 3951 Size: 398 274 147 411 4288 Fragments arranged by size: 4288 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5293 Fragments arranged by size: 5293 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2552 1186 Size: 1366 4152 BseYI C'CCAG_C Cuts at: 2402 4721 5217 2402 Size: 2319 496 2703 Fragments arranged by size: 2703 2319 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2023 2632 2645 1163 Size: 573 287 609 13 4036 Fragments arranged by size: 4036 609 573 287 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2197 2597 3579 3664 4735 2197 Size: 400 982 85 1071 2980 Fragments arranged by size: 2980 1071 982 400 85 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 2597 2837 3579 3664 4735 1391 Size: 399 407 400 240 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 407 400 399 240 85 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2574 2873 844 Size: 1730 299 3489 Fragments arranged by size: 3489 1730 299 BsrBI CCG'CTC Cuts at: 1394 1823 2589 3405 1394 Size: 429 766 816 3507 Fragments arranged by size: 3507 816 766 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4162 4349 1700 Size: 110 2352 187 2869 Fragments arranged by size: 2869 2352 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 2712 794 Size: 1918 3600 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstXI CCAn_nnnn'nTGG Cuts at: 2402 2795 2402 Size: 393 5125 BstYI r'GATC_y Cuts at: 1972 2808 2848 3215 3600 3617 5069 5155 5167 Size: 836 40 367 385 17 1452 86 12 Cuts at: 5167 5513 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 836 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2617 513 Size: 552 1552 3414 Fragments arranged by size: 3414 1552 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4779 Fragments arranged by size: 4779 683 56 BtsI GCAGTG_nn' Cuts at: 2746 3034 3828 3848 2746 Size: 288 794 20 4416 Fragments arranged by size: 4416 794 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2132 2167 529 Size: 35 1568 35 3880 Fragments arranged by size: 3880 1568 35 35 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483 Size: 54 1425 50 275 18 1135 889 1629 Cuts at: 5483 8 Size: 43 Fragments arranged by size: 1629 1425 1135 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 3448 1353 Size: 753 1342 3423 Fragments arranged by size: 3423 1342 753 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2195 2195 Size: 5518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2205 2474 2504 2539 2702 3581 Size: 393 414 159 269 30 35 163 879 Cuts at: 3581 4178 4965 5233 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 414 393 269 268 163 159 35 30 EcoNI CCTnn'n_nnAGG Cuts at: 2103 2103 Size: 5518 EcoO109I rG'GnC_Cy Cuts at: 2546 2657 2759 2546 Size: 111 102 5305 Fragments arranged by size: 5305 111 102 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2183 2183 Size: 5518 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 4665 1678 Size: 2987 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2280 2312 4159 Size: 32 135 32 576 32 100 32 1847 Cuts at: 4159 4191 4233 4265 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1847 576 135 100 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 2146 748 Size: 1398 4120 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2934 3611 4759 5004 1606 Size: 486 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 486 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4966 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2143 2876 4421 1132 Size: 97 914 733 1545 2229 Fragments arranged by size: 2229 1545 914 733 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 1108 Size: 1374 4144 PfoI T'CCnGG_A Cuts at: 2718 2718 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2657 2759 2657 Size: 102 5416 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PstI C_TGCA'G Cuts at: 830 2091 2734 2741 2870 830 Size: 1261 643 7 129 3478 Fragments arranged by size: 3478 1261 643 129 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2092 2092 Size: 5518 SacI G_AGCT'C Cuts at: 2197 2197 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SanDI GG'GwC_CC Cuts at: 2657 2657 Size: 5518 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SexAI A'CCwGG_T Cuts at: 2113 2113 Size: 5518 SfcI C'TryA_G Cuts at: 826 1045 2087 2315 2730 2737 2866 4001 4682 Size: 219 1042 228 415 7 129 1135 681 Cuts at: 4682 4873 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1042 681 415 228 219 191 129 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2845 1872 Size: 973 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5518 StyI C'CwwG_G Cuts at: 513 1065 2957 513 Size: 552 1892 3074 Fragments arranged by size: 3074 1892 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 3562 3715 3900 4345 325 Size: 1255 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2487 2519 5478 5510 2487 Size: 32 2959 32 2495 Fragments arranged by size: 2959 2495 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2350 2461 2854 2350 Size: 111 393 5014 Fragments arranged by size: 5014 393 111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2843 1870 Size: 973 4545 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI