Restriction map for
pFN21AE0449
(Linear) (Six-base) MAPSORT of: KIEE0449.seq Check: 2144 from: 1 to: 813
KIEE0449.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 282 813
Size: 282 531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 200 469 534 813
Size: 41 159 269 65 279
Fragments arranged by size:
279 269 159 65 41
AhdI GACnn_n'nnGTC
Cuts at: 0 289 813
Size: 289 524
AlwNI CAG_nnn'CTG
Cuts at: 0 244 707 813
Size: 244 463 106
Fragments arranged by size:
463 244 106
AvaI C'yCGr_G
Cuts at: 0 66 627 813
Size: 66 561 186
Fragments arranged by size:
561 186 66
BanII G_rGCy'C
Cuts at: 0 192 813
Size: 192 621
BbvCI CC'TCA_GC
Cuts at: 0 748 813
Size: 748 65
BglII A'GATC_T
Cuts at: 0 803 813
Size: 803 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 697 813
Size: 499 198 116
Fragments arranged by size:
499 198 116
Bpu10I CC'TnA_GC
Cuts at: 0 748 813
Size: 748 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 813
Size: 347 466
BsaHI Gr'CG_yC
Cuts at: 0 50 285 813
Size: 50 235 528
Fragments arranged by size:
528 235 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 813
Size: 547 266
BseYI C'CCAG_C
Cuts at: 0 397 813
Size: 397 416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 627 640 813
Size: 18 609 13 173
Fragments arranged by size:
609 173 18 13
BsiHKAI G_wGCw'C
Cuts at: 0 192 592 813
Size: 192 400 221
Fragments arranged by size:
400 221 192
Bsp1286I G_dGCh'C
Cuts at: 0 192 592 813
Size: 192 400 221
Fragments arranged by size:
400 221 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 569 813
Size: 569 244
BsrBI CCG'CTC
Cuts at: 0 584 813
Size: 584 229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 707 813
Size: 707 106
BstXI CCAn_nnnn'nTGG
Cuts at: 0 397 790 813
Size: 397 393 23
Fragments arranged by size:
397 393 23
BstYI r'GATC_y
Cuts at: 0 803 813
Size: 803 10
BtgI C'CryG_G
Cuts at: 0 612 813
Size: 612 201
BtsI GCAGTG_nn'
Cuts at: 0 741 813
Size: 741 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 127 162 813
Size: 127 35 651
Fragments arranged by size:
651 127 35
EarI CTCTTCn'nnn_
Cuts at: 0 101 813
Size: 101 712
EcoICRI GAG'CTC
Cuts at: 0 190 813
Size: 190 623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 200 469 499 534 697 813
Size: 41 159 269 30 35 163 116
Fragments arranged by size:
269 163 159 116 41 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 98 813
Size: 98 715
EcoO109I rG'GnC_Cy
Cuts at: 0 541 652 754 813
Size: 541 111 102 59
Fragments arranged by size:
541 111 102 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 178 813
Size: 178 635
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 275 307 813
Size: 143 32 100 32 506
Fragments arranged by size:
506 143 100 32 32
HindIII A'AGCT_T
Cuts at: 0 141 813
Size: 141 672
MspA1I CmG'CkG
Cuts at: 0 87 813
Size: 87 726
NspI r_CATG'y
Cuts at: 0 138 813
Size: 138 675
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 813
Size: 477 336
PfoI T'CCnGG_A
Cuts at: 0 713 813
Size: 713 100
PpuMI rG'GwC_Cy
Cuts at: 0 652 754 813
Size: 652 102 59
Fragments arranged by size:
652 102 59
PstI C_TGCA'G
Cuts at: 0 86 729 736 813
Size: 86 643 7 77
Fragments arranged by size:
643 86 77 7
PvuII CAG'CTG
Cuts at: 0 87 813
Size: 87 726
SacI G_AGCT'C
Cuts at: 0 192 813
Size: 192 621
SanDI GG'GwC_CC
Cuts at: 0 652 813
Size: 652 161
SexAI A'CCwGG_T
Cuts at: 0 108 813
Size: 108 705
SfcI C'TryA_G
Cuts at: 0 82 310 725 732 813
Size: 82 228 415 7 81
Fragments arranged by size:
415 228 82 81 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 482 514 813
Size: 482 32 299
Fragments arranged by size:
482 299 32
XbaI T'CTAG_A
Cuts at: 0 345 456 813
Size: 345 111 357
Fragments arranged by size:
357 345 111
Enzymes that do cut:
AccI AcuI AhdI AlwNI AvaI BanII BbvCI BglII
BpmI Bpu10I BsaI BsaHI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BstAPI BstXI BstYI BtgI BtsI
CspCI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HindIII MspA1I NspI PflMI PfoI PpuMI PstI PvuII
SacI SanDI SexAI SfcI TstI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XcmI XhoI XmaI XmnI ZraI