(Circular) (Six-base) MAPSORT of: pFN21AB7316.seq Check: 9658 from: 1 to: 7996 pFN21AB7316 7996 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5806 278 Size: 53 83 186 936 4270 2468 Fragments arranged by size: 4270 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5339 1937 Size: 3402 4594 Acc65I G'GTAC_C Cuts at: 5317 5317 Size: 7996 AclI AA'CG_TT Cuts at: 1563 2397 6123 6496 1563 Size: 834 3726 373 3063 Fragments arranged by size: 3726 3063 834 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3180 4824 6059 7443 7711 1632 Size: 1548 1644 1235 1384 268 1917 Fragments arranged by size: 1917 1644 1548 1384 1235 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7799 AflIII A'CryG_T Cuts at: 1203 6895 7872 1203 Size: 5692 977 1327 Fragments arranged by size: 5692 1327 977 AgeI A'CCGG_T Cuts at: 6827 6827 Size: 7996 AhdI GACnn_n'nnGTC Cuts at: 6725 6725 Size: 7996 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7996 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7996 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3520 3552 3520 Size: 32 7964 AlwNI CAG_nnn'CTG Cuts at: 2089 4544 4793 4826 5739 7311 2089 Size: 2455 249 33 913 1572 2774 Fragments arranged by size: 2774 2455 1572 913 249 33 ApaI G_GGCC'C Cuts at: 3095 4466 3095 Size: 1371 6625 ApaLI G'TGCA_C Cuts at: 6053 7209 6053 Size: 1156 6840 ApoI r'AATT_y Cuts at: 1493 2077 2301 2341 2375 2431 3469 3655 3835 Size: 584 224 40 34 56 1038 186 180 Cuts at: 3835 3879 4189 4498 5116 5305 5531 5683 7792 Size: 44 310 309 618 189 226 152 2109 Cuts at: 7792 1493 Size: 1697 Fragments arranged by size: 2109 1697 1038 618 584 310 309 226 224 189 186 180 152 56 44 40 34 AseI AT'TA_AT Cuts at: 160 1032 3336 3390 3687 4511 5031 5193 6550 Size: 872 2304 54 297 824 520 162 1357 Cuts at: 6550 160 Size: 1606 Fragments arranged by size: 2304 1606 1357 872 824 520 297 162 54 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7996 AvaI C'yCGr_G Cuts at: 1870 1943 5321 1870 Size: 73 3378 4545 Fragments arranged by size: 4545 3378 73 BamHI G'GATC_C Cuts at: 5326 5693 5326 Size: 367 7629 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5317 6772 618 Size: 325 217 627 3530 1455 1842 Fragments arranged by size: 3530 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2152 2556 2723 3095 3848 4466 5315 1391 Size: 761 404 167 372 753 618 849 4072 Fragments arranged by size: 4072 849 761 753 618 404 372 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4245 4391 5235 928 Size: 971 2346 146 844 3689 Fragments arranged by size: 3689 2346 971 844 146 BbvCI CC'TCA_GC Cuts at: 3774 3774 Size: 7996 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6187 6221 1903 Size: 34 4250 34 3678 Fragments arranged by size: 4250 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2736 4198 4580 5888 7098 2736 Size: 1462 382 1308 1210 3634 Fragments arranged by size: 3634 1462 1308 1210 382 BclI T'GATC_A Cuts at: 1548 1548 Size: 7996 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6607 136 Size: 107 122 71 631 605 4935 1525 Fragments arranged by size: 4935 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2424 7991 2424 Size: 5567 2429 BlpI GC'TnA_GC Cuts at: 4794 5413 4794 Size: 619 7377 Bme1580I G_kGCm'C Cuts at: 1790 3095 4466 6057 7213 1790 Size: 1305 1371 1591 1156 2573 Fragments arranged by size: 2573 1591 1371 1305 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4526 4781 5727 Size: 416 53 83 399 24 3109 255 946 Cuts at: 5727 6685 442 Size: 958 1753 Fragments arranged by size: 3109 1753 958 946 416 399 255 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7996 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5185 5185 Size: 7996 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2403 2632 2737 4045 4923 5112 6656 1239 Size: 1164 229 105 1308 878 189 1544 2579 Fragments arranged by size: 2579 1544 1308 1164 878 229 189 105 Bpu10I CC'TnA_GC Cuts at: 3774 3774 Size: 7996 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2757 4938 6121 6986 7284 2757 Size: 2181 1183 865 298 3469 Fragments arranged by size: 3469 2181 1183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2702 6659 882 Size: 617 1203 3957 2219 Fragments arranged by size: 3957 2219 1203 617 BsaAI yAC'GTr Cuts at: 493 1204 2020 2537 3098 5787 493 Size: 711 816 517 561 2689 2702 Fragments arranged by size: 2702 2689 816 711 561 517 BsaBI GATnn'nnATC Cuts at: 3262 7990 3262 Size: 4728 3268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5803 6185 6820 275 Size: 53 83 186 936 4270 382 635 1451 Fragments arranged by size: 4270 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6429 6827 7101 7248 7659 6429 Size: 398 274 147 411 6766 Fragments arranged by size: 6766 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7771 Fragments arranged by size: 7771 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 3000 3926 5265 1186 Size: 956 858 926 1339 3917 Fragments arranged by size: 3917 1339 956 926 858 BseYI C'CCAG_C Cuts at: 3250 4885 7199 7695 3250 Size: 1635 2314 496 3551 Fragments arranged by size: 3551 2314 1635 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4896 1163 Size: 573 3160 4263 Fragments arranged by size: 4263 3160 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4004 5446 6207 7235 1540 Size: 275 186 2003 1442 761 1028 2301 Fragments arranged by size: 2301 2003 1442 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2556 4687 6057 6142 7213 2556 Size: 2131 1370 85 1071 3339 Fragments arranged by size: 3339 2131 1370 1071 85 BsmI GAATG_Cn' Cuts at: 3241 5507 5600 3241 Size: 2266 93 5637 Fragments arranged by size: 5637 2266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4002 4002 Size: 7996 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2556 2723 3095 3848 4466 4687 Size: 399 362 404 167 372 753 618 221 Cuts at: 4687 5315 6057 6142 7213 1391 Size: 628 742 85 1071 2174 Fragments arranged by size: 2174 1071 753 742 628 618 404 399 372 362 221 167 85 BspEI T'CCGG_A Cuts at: 7659 7659 Size: 7996 BspHI T'CATG_A Cuts at: 2155 5885 7615 2155 Size: 3730 1730 2536 Fragments arranged by size: 3730 2536 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5351 844 Size: 4507 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2505 5883 1394 Size: 429 682 3378 3507 Fragments arranged by size: 3507 3378 682 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6491 6665 65 Size: 1183 5243 174 1396 Fragments arranged by size: 5243 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6640 6827 1700 Size: 110 4830 187 2869 Fragments arranged by size: 4830 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7996 BssSI C'ACGA_G Cuts at: 6056 7068 6056 Size: 1012 6984 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7996 BstBI TT'CG_AA Cuts at: 6800 6800 Size: 7996 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7996 BstXI CCAn_nnnn'nTGG Cuts at: 2882 2882 Size: 7996 BstYI r'GATC_y Cuts at: 1972 2424 5141 5326 5693 6078 6095 7547 7633 Size: 452 2717 185 367 385 17 1452 86 Cuts at: 7633 7645 7991 1972 Size: 12 346 1977 Fragments arranged by size: 2717 1977 1452 452 385 367 346 185 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5094 5094 Size: 7996 BtgI C'CryG_G Cuts at: 513 1065 2227 513 Size: 552 1162 6282 Fragments arranged by size: 6282 1162 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7257 Fragments arranged by size: 7257 683 56 BtsI GCAGTG_nn' Cuts at: 4545 4687 5512 6306 6326 4545 Size: 142 825 794 20 6215 Fragments arranged by size: 6215 825 794 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7996 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7961 DraI TTT'AAA Cuts at: 3042 5300 3042 Size: 2258 5738 DraIII CAC_nnn'GTG Cuts at: 2321 2321 Size: 7996 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7003 809 Size: 507 5687 1802 Fragments arranged by size: 5687 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2851 5443 6332 Size: 54 1425 50 275 18 1021 2592 889 Cuts at: 6332 7961 8 Size: 1629 43 Fragments arranged by size: 2592 1629 1425 1021 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5443 1537 Size: 275 3631 4090 Fragments arranged by size: 4090 3631 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2697 4466 5055 5926 1353 Size: 1344 1769 589 871 3423 Fragments arranged by size: 3423 1769 1344 871 589 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6583 6957 7103 387 Size: 1064 138 4994 374 146 1280 Fragments arranged by size: 4994 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2554 2554 Size: 7996 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2632 2737 3180 4045 4824 4923 Size: 393 771 229 105 443 865 779 99 Cuts at: 4923 5112 6059 6656 7443 7711 1239 Size: 189 947 597 787 268 1524 Fragments arranged by size: 1524 947 865 787 779 771 597 443 393 268 229 189 105 99 EcoNI CCTnn'n_nnAGG Cuts at: 4992 4992 Size: 7996 EcoO109I rG'GnC_Cy Cuts at: 2262 3617 2262 Size: 1355 6641 EcoRI G'AATT_C Cuts at: 5305 5683 5305 Size: 378 7618 EcoRV GAT'ATC Cuts at: 2996 4130 2996 Size: 1134 6862 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2618 2804 3129 2618 Size: 186 325 7485 Fragments arranged by size: 7485 325 186 FspI TGC'GCA Cuts at: 6502 6502 Size: 7996 HaeII r_GCGC'y Cuts at: 1678 7143 1678 Size: 5465 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4922 4954 5216 5248 6637 Size: 32 135 32 3350 32 262 32 1389 Cuts at: 6637 6669 6711 6743 1373 Size: 32 42 32 2626 Fragments arranged by size: 3350 2626 1389 262 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5340 5586 669 Size: 988 281 3402 246 3079 Fragments arranged by size: 3402 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7996 HpaI GTT'AAC Cuts at: 5586 5586 Size: 7996 KpnI G_GTAC'C Cuts at: 5321 5321 Size: 7996 MfeI C'AATT_G Cuts at: 5595 5595 Size: 7996 MluI A'CGCG_T Cuts at: 7872 7872 Size: 7996 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 3041 3984 4534 7110 7294 1305 Size: 957 779 943 550 2576 184 2007 Fragments arranged by size: 2576 2007 957 943 779 550 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2853 10 Size: 54 1425 343 1021 5153 Fragments arranged by size: 5153 1425 1021 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2880 4247 4532 5954 6313 6472 518 Size: 648 1714 1367 285 1422 359 159 2042 Fragments arranged by size: 2042 1714 1422 1367 648 359 285 159 MspA1I CmG'CkG Cuts at: 1606 3250 3347 3442 5412 6089 7237 7482 1606 Size: 1644 97 95 1970 677 1148 245 2120 Fragments arranged by size: 2120 1970 1644 1148 677 245 97 95 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7444 NdeI CA'TA_TG Cuts at: 387 387 Size: 7996 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7996 NotI GC'GGCC_GC Cuts at: 5443 5443 Size: 7996 NspI r_CATG'y Cuts at: 1132 1229 5354 6899 1132 Size: 97 4125 1545 2229 Fragments arranged by size: 4125 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7996 PciI A'CATG_T Cuts at: 6895 6895 Size: 7996 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7996 PfoI T'CCnGG_A Cuts at: 2039 3067 2039 Size: 1028 6968 PmeI GTTT'AAAC Cuts at: 5300 5300 Size: 7996 PmlI CAC'GTG Cuts at: 3098 3098 Size: 7996 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6065 6097 7578 7610 6065 Size: 32 1481 32 6451 Fragments arranged by size: 6451 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2262 2262 Size: 7996 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7996 PsiI TTA'TAA Cuts at: 5566 5566 Size: 7996 PspOMI G'GGCC_C Cuts at: 3091 4462 3091 Size: 1371 6625 PstI C_TGCA'G Cuts at: 830 4216 5059 5348 830 Size: 3386 843 289 3478 Fragments arranged by size: 3478 3386 843 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7996 PvuII CAG'CTG Cuts at: 3347 3347 Size: 7996 SacI G_AGCT'C Cuts at: 2556 2556 Size: 7996 SalI G'TCGA_C Cuts at: 1936 5338 1936 Size: 3402 4594 SbfI CC_TGCA'GG Cuts at: 5348 5348 Size: 7996 ScaI AGT'ACT Cuts at: 6244 7956 6244 Size: 1712 6284 SexAI A'CCwGG_T Cuts at: 4081 4428 4081 Size: 347 7649 SfcI C'TryA_G Cuts at: 826 1045 4212 4989 5055 5344 6479 7160 7351 Size: 219 3167 777 66 289 1135 681 191 Cuts at: 7351 826 Size: 1471 Fragments arranged by size: 3167 1471 1135 777 681 289 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7996 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7996 SmaI CCC'GGG Cuts at: 1872 5323 1872 Size: 3451 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2772 4953 6100 7001 7263 820 Size: 197 926 829 2181 1147 901 262 1553 Fragments arranged by size: 2181 1553 1147 926 901 829 262 197 SnaBI TAC'GTA Cuts at: 493 2020 2537 5787 493 Size: 1527 517 3250 2702 Fragments arranged by size: 3250 2702 1527 517 SpeI A'CTAG_T Cuts at: 152 152 Size: 7996 SphI G_CATG'C Cuts at: 1132 5354 1132 Size: 4222 3774 SspI AAT'ATT Cuts at: 5 52 2914 4366 5920 5 Size: 47 2862 1452 1554 2081 Fragments arranged by size: 2862 2081 1554 1452 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7996 StyI C'CwwG_G Cuts at: 513 1065 2634 5435 513 Size: 552 1569 2801 3074 Fragments arranged by size: 3074 2801 1569 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3990 4873 6023 6040 6193 6378 6823 Size: 1255 2410 883 1150 17 153 185 445 Cuts at: 6823 325 Size: 1498 Fragments arranged by size: 2410 1498 1255 1150 883 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3430 6242 7954 Size: 274 80 33 51 1443 1453 2812 1712 Cuts at: 7954 96 Size: 138 Fragments arranged by size: 2812 1712 1453 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7956 7988 7956 Size: 32 7964 Tth111I GACn'n_nGTC Cuts at: 1316 3974 1316 Size: 2658 5338 XbaI T'CTAG_A Cuts at: 5332 5332 Size: 7996 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2653 2653 Size: 7996 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7996 XmaI C'CCGG_G Cuts at: 1870 5321 1870 Size: 3451 4545 XmnI GAAnn'nnTTC Cuts at: 4242 6125 4242 Size: 1883 6113 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5804 276 Size: 53 83 186 936 4270 2468 Fragments arranged by size: 4270 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI