################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 362 GCGG CCGC(-3/-1) AcsI 274 AAATTC [GA]^AATT[CT] AflIII 371 ACATGT A^C[GA][CT]GT AlwNI 186 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 274 AAATTC [GA]^AATT[CT] AseI 97 ATTAAT AT^TAAT AsnI 97 ATTAAT AT^TAAT BcnI 40 CCGGG CC^[GC]GG BglI 291 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 298 GGGCAC G[AGT]GC[ACT]^C BsmFI 112 GGGAC GGGAC(10/14) Bsp1286I 298 GGGCAC G[AGT]GC[ACT]^C BspLU11I 371 ACATGT A^CATGT BspMI 128 ACCTGC ACCTGC(4/8) BsrI 191 ACTGG ACTGG(1/-1) BssSI 301 CACGAG CACGAG(-5/-1) BstXI 317 CCAACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 105 GGGCC G^G[ACGT]CC FauI 362 GCGGG CCCGC(4/6) HaeIII 106 GGCC GG^CC HapII 40 CCGG C^CGG HincII 45 GTCAAC GT[CT]^[GA]AC HindII 45 GTCAAC GT[CT]^[GA]AC HinfI 154 GAATC G^A[ACGT]TC HpaII 40 CCGG C^CGG MslI 27 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 40 CCGG C^CGG NciI 40 CCGGG CC^[GC]GG NspI 371 ACATGT [GA]CATG^[CT] PshBI 97 ATTAAT AT^TAAT Sau96I 105 GGGCC G^G[ACGT]CC SfaNI 68 GCATC GCATC(5/9) SmlI 13 CTTGAG C^T[CT][GA]AG TfiI 154 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BstNI 108 CCAGG CC^[AT]GG 232 CCAGG DdeI 340 CTGAG C^T[ACGT]AG 349 CTGAG DpnII 57 GATC ^GATC 214 GATC EcoRII 108 CCAGG ^CC[AT]GG 232 CCAGG HphI 282 GGTGA GGTGA(8/7) 216 TCACC MboI 57 GATC ^GATC 214 GATC MboII 54 GAAGA GAAGA(8/7) 211 GAAGA MvaI 108 CCAGG CC^[AT]GG 232 CCAGG NdeII 57 GATC ^GATC 214 GATC Sau3AI 57 GATC ^GATC 214 GATC Tsp509I 96 AATT ^AATT 275 AATT TspRI 81 CACTG CA[GC]TG[ACGT][ACGT]^ 254 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BsaJI BssKI Fnu4HI ItaI MaeIII MnlI MseI MwoI NlaIII ScrFI Tru9I TseI Tsp45I