(Circular) (Six-base) MAPSORT of: pFN21AE0375.seq Check: 5018 from: 1 to: 6406 pFN21AE0375 6406 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2371 2371 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2208 4216 278 Size: 53 83 186 936 672 2008 2468 Fragments arranged by size: 2468 2008 936 672 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 3749 1937 Size: 1422 390 4594 Fragments arranged by size: 4594 1422 390 Acc65I G'GTAC_C Cuts at: 3540 3634 3727 3540 Size: 94 93 6219 Fragments arranged by size: 6219 94 93 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2521 2681 3456 4469 5853 6121 1632 Size: 889 160 775 1013 1384 268 1917 Fragments arranged by size: 1917 1384 1013 889 775 268 160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 2968 5237 2968 Size: 2269 4137 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3053 3085 3053 Size: 32 6374 AlwNI CAG_nnn'CTG Cuts at: 4149 5721 4149 Size: 1572 4834 ApaI G_GGCC'C Cuts at: 3084 3084 Size: 6406 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2402 2775 3254 3288 3434 3715 3941 4093 Size: 909 373 479 34 146 281 226 152 Cuts at: 4093 6202 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 909 479 373 281 226 152 146 34 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2610 3731 1870 Size: 73 667 1121 4545 Fragments arranged by size: 4545 1121 667 73 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2165 2672 3540 3634 Size: 325 217 627 263 115 507 868 94 Cuts at: 3634 3727 5182 618 Size: 93 1455 1842 Fragments arranged by size: 1842 1455 868 627 507 325 263 217 115 94 93 BanII G_rGCy'C Cuts at: 1391 3084 3597 3725 1391 Size: 1693 513 128 4072 Fragments arranged by size: 4072 1693 513 128 BbeI G_GCGC'C Cuts at: 2169 2169 Size: 6406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 928 Size: 971 301 5134 Fragments arranged by size: 5134 971 301 BbvCI CC'TCA_GC Cuts at: 2303 2303 Size: 6406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 3395 4298 5508 3299 Size: 96 903 1210 4197 Fragments arranged by size: 4197 1210 903 96 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3136 6401 3136 Size: 3265 3141 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2055 3084 4467 5623 1790 Size: 265 1029 1383 1156 2573 Fragments arranged by size: 2573 1383 1156 1029 265 BmgBI CAC'GTC Cuts at: 1318 2317 1318 Size: 999 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2479 2803 4137 Size: 416 53 83 399 24 1062 324 1334 Cuts at: 4137 5095 442 Size: 958 1753 Fragments arranged by size: 1753 1334 1062 958 416 399 324 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2966 2972 5066 1239 Size: 1235 492 6 2094 2579 Fragments arranged by size: 2579 2094 1235 492 6 Bpu10I CC'TnA_GC Cuts at: 2303 2303 Size: 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3375 5069 882 Size: 617 1876 1694 2219 Fragments arranged by size: 2219 1876 1694 617 BsaAI yAC'GTr Cuts at: 493 1204 2745 4197 493 Size: 711 1541 1452 2702 Fragments arranged by size: 2702 1541 1452 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2205 4213 4595 Size: 53 83 186 936 633 39 2008 382 Cuts at: 4595 5230 275 Size: 635 1451 Fragments arranged by size: 2008 1451 936 635 633 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 2968 4839 5237 5511 5658 6069 2968 Size: 1871 398 274 147 411 3305 Fragments arranged by size: 3305 1871 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 2472 2502 3053 Size: 30 165 30 915 30 124 30 551 Cuts at: 3053 3083 3669 3699 1178 Size: 30 586 30 3885 Fragments arranged by size: 3885 915 586 551 165 124 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3677 3709 1186 Size: 2491 32 3883 Fragments arranged by size: 3883 2491 32 BseYI C'CCAG_C Cuts at: 2059 3083 5609 6105 2059 Size: 1024 2526 496 2360 Fragments arranged by size: 2526 2360 1024 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2958 1163 Size: 573 561 661 4611 Fragments arranged by size: 4611 661 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3856 4617 5645 1540 Size: 275 186 1099 756 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 756 275 186 BsiHKAI G_wGCw'C Cuts at: 4467 4552 5623 4467 Size: 85 1071 5250 Fragments arranged by size: 5250 1071 85 BsmI GAATG_Cn' Cuts at: 2728 3917 4010 2728 Size: 1189 93 5124 Fragments arranged by size: 5124 1189 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2240 2630 2240 Size: 390 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 3084 3597 3725 4467 4552 5623 Size: 399 265 1029 513 128 742 85 1071 Cuts at: 5623 1391 Size: 2174 Fragments arranged by size: 2174 1071 1029 742 513 399 265 128 85 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 2910 4295 6025 2910 Size: 1385 1730 3291 Fragments arranged by size: 3291 1730 1385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2371 3761 844 Size: 1527 1390 3489 Fragments arranged by size: 3489 1527 1390 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2622 3498 4901 5075 65 Size: 1183 1374 876 1403 174 1396 Fragments arranged by size: 1403 1396 1374 1183 876 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2675 2891 2968 5050 5237 1700 Size: 110 865 216 77 2082 187 2869 Fragments arranged by size: 2869 2082 865 216 187 110 77 BsrGI T'GTAC_A Cuts at: 96 2682 96 Size: 2586 3820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6406 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 2964 1184 Size: 1780 4626 BstYI r'GATC_y Cuts at: 1972 2473 3136 3736 4103 4488 4505 5957 6043 Size: 501 663 600 367 385 17 1452 86 Cuts at: 6043 6055 6401 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 663 600 501 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3360 3360 Size: 6406 Bsu36I CC'TnA_GG Cuts at: 3692 3692 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2668 3167 513 Size: 552 1603 499 3752 Fragments arranged by size: 3752 1603 552 499 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 3666 3922 4716 4736 3666 Size: 256 794 20 5336 Fragments arranged by size: 5336 794 256 20 ClaI AT'CG_AT Cuts at: 1554 2421 1554 Size: 867 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2485 2520 3510 3545 529 Size: 35 1921 35 990 35 3390 Fragments arranged by size: 3390 1921 990 35 35 35 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DraIII CAC_nnn'GTG Cuts at: 2219 2745 2219 Size: 526 5880 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 3131 3853 Size: 54 1425 50 275 18 696 605 722 Cuts at: 3853 4742 6371 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 722 696 605 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2984 4336 1353 Size: 729 902 1352 3423 Fragments arranged by size: 3423 1352 902 729 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2521 2681 2966 2972 3456 4469 Size: 393 842 47 160 285 6 484 1013 Cuts at: 4469 5066 5853 6121 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1013 842 787 597 484 393 285 268 160 47 6 EcoNI CCTnn'n_nnAGG Cuts at: 2684 2684 Size: 6406 EcoO109I rG'GnC_Cy Cuts at: 2079 2189 2827 3080 2079 Size: 110 638 253 5405 Fragments arranged by size: 5405 638 253 110 EcoRI G'AATT_C Cuts at: 2402 3288 3715 4093 2402 Size: 886 427 378 4715 Fragments arranged by size: 4715 886 427 378 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 2169 5553 1678 Size: 491 3384 2531 Fragments arranged by size: 3384 2531 491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3128 3160 5047 5079 5121 Size: 32 135 32 1556 32 1887 32 42 Cuts at: 5121 5153 1373 Size: 32 2626 Fragments arranged by size: 2626 1887 1556 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 3121 3750 3996 669 Size: 988 281 382 801 629 246 3079 Fragments arranged by size: 3079 988 801 629 382 281 246 HindIII A'AGCT_T Cuts at: 748 3549 748 Size: 2801 3605 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KasI G'GCGC_C Cuts at: 2165 2165 Size: 6406 KpnI G_GTAC'C Cuts at: 3544 3638 3731 3544 Size: 94 93 6219 Fragments arranged by size: 6219 94 93 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3357 5520 5704 1305 Size: 2052 2163 184 2007 Fragments arranged by size: 2163 2052 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 3133 10 Size: 54 1425 343 696 605 3283 Fragments arranged by size: 3283 1425 696 605 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3227 3614 4364 4723 4882 518 Size: 648 2061 387 750 359 159 2042 Fragments arranged by size: 2061 2042 750 648 387 359 159 MspA1I CmG'CkG Cuts at: 1606 2485 2670 3822 4499 5647 5892 1606 Size: 879 185 1152 677 1148 245 2120 Fragments arranged by size: 2120 1152 1148 879 677 245 185 NarI GG'CG_CC Cuts at: 2166 2166 Size: 6406 NcoI C'CATG_G Cuts at: 513 1065 3167 513 Size: 552 2102 3752 Fragments arranged by size: 3752 2102 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3613 3764 5309 1132 Size: 97 2384 151 1545 2229 Fragments arranged by size: 2384 2229 1545 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2724 1108 Size: 1616 4790 PfoI T'CCnGG_A Cuts at: 2283 2982 2283 Size: 699 5707 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PmlI CAC'GTG Cuts at: 2745 2745 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 2133 3053 3085 4475 4507 5988 6020 2101 Size: 32 920 32 1390 32 1481 32 2487 Fragments arranged by size: 2487 1481 1390 920 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2079 2827 2079 Size: 748 5658 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 2736 3976 2736 Size: 1240 5166 PspOMI G'GGCC_C Cuts at: 3080 3080 Size: 6406 PstI C_TGCA'G Cuts at: 830 3592 3758 830 Size: 2762 166 3478 Fragments arranged by size: 3478 2762 166 PvuI CG_AT'CG Cuts at: 2001 3100 2001 Size: 1099 5307 PvuII CAG'CTG Cuts at: 2485 2485 Size: 6406 SacII CC_GC'GG Cuts at: 2671 2671 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SanDI GG'GwC_CC Cuts at: 2079 2079 Size: 6406 SapI GCTCTTCn'nnn_ Cuts at: 2984 2984 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 2407 2852 3588 3754 4889 5570 5761 Size: 219 1362 445 736 166 1135 681 191 Cuts at: 5761 826 Size: 1471 Fragments arranged by size: 1471 1362 1135 736 681 445 219 191 166 SfoI GGC'GCC Cuts at: 2167 2167 Size: 6406 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4510 5411 5673 820 Size: 197 926 2567 901 262 1553 Fragments arranged by size: 2567 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 3482 4330 5 Size: 47 3430 848 2081 Fragments arranged by size: 3430 2081 848 47 StuI AGG'CCT Cuts at: 1517 2093 2567 2949 1517 Size: 576 474 382 4974 Fragments arranged by size: 4974 576 474 382 StyI C'CwwG_G Cuts at: 513 1065 2823 3167 3845 513 Size: 552 1758 344 678 3074 Fragments arranged by size: 3074 1758 678 552 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2496 4433 4450 4603 4788 5233 325 Size: 1255 916 1937 17 153 185 445 1498 Fragments arranged by size: 1937 1498 1255 916 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 3263 4652 Size: 274 80 33 51 1443 705 581 1389 Cuts at: 4652 6364 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1389 705 581 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2472 2504 6366 6398 2472 Size: 32 3862 32 2480 Fragments arranged by size: 3862 2480 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3229 3229 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2206 4214 276 Size: 53 83 186 936 672 2008 2468 Fragments arranged by size: 2468 2008 936 672 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI